CS217459B1 - Method of antimicrobial treatment of substances by cold sterilization - Google Patents

Abstract

The invention relates to cold sterilization of substances with peroxyacetic acid, where active oxygen residues are destroyed by reducing agents. Detection of a suitable product is carried out with an iodine starch paper. The problem of antimicrobial treatment of substances in the pharmaceutical and food industries as well as in agriculture or in other sectors of the national economy is increasingly relevant. Peroxyacetic acid, which is a well-known antimicrobial agent, particularly meets these requirements. The disadvantage is the residues of the said acid, which can be harmful to health. The said invention solves this problem by adding a reducing agent to the treated substance - peroxyacetic acid system after an appropriate exposure time. The destruction of peroxyacetic acid is detected with an iodine starch indicator. The decomposition products of peroxyacetic acid and the reducing agent are in minimal concentration and are harmless to health.

Description

2

The invention relates to a method of antimicroibial treatment of substances by cold sterilization.

For the preparation of medicaments, medicinal products, food products and feeds, it is necessary to start from microbially harmless substances.

Permitted amounts of microbial germs are indicated by applicable standards. Preservatives prevent the growth and multiplication of siamicrobes, but ioh effects are limited, especially for the recovery of state-microbial contamination of the conserved system. For this reason, it is preferred to reduce the amount of microbial germs to as low as possible. In contrast to the definition of sterilization, it is preferred to sterilize the materials prior to preservation. According to the author Wiallussussas, sterilization means the killing of all microorganisms and the inactivation of all viruses, with no eligible cells in the sterilized system (Wal'1-ihussusser, KH, Sterilization - Desinfecti - Konsenvierung, 2. Aufl., Thieme Verlag) , Stuttgart 1978, p. 193).

Among the most widely used procedures are thermal sterilization, which has many modifications to its specific design - sterilization by dry heat, damp heat, autoclave, heat-air and flame. Many substances and devices cannot even be exposed to thermal stress for a short period of time, therefore gases and a pair of substances with suitable antimicrobial activity (ethylene oxide, beta-propyl lactome, ozone, peracids) are also used for sterilization. Another sterilization procedure is gamma irradiation with appropriate penetration and ultra-violet irradiation with suitable wavelength (germicidal radiation). A particular sterilization procedure is sterile filtration, which is one of the so-called aseptic work.

In the state of the art, it is possible to sterilize the extractive substances (possibly mono-products) with peroxyacids (peroxygenic acid, peroxybenzoic acid), since peroxyacetic acid is the most commonly used for practical purposes (about 40% is marketed) solution in water under the name Peristil. The substances to be sterilized are not uniformly homogenized by the homogenizer and then the peroxyacetate is added at a concentration of 0.001% -1% by weight, calculated as 100% peracetic acid. The time for which the substances will be sterilized depends on the depletion of the contaminants of the microorganisms from the temperature at which they will be sterilized. It is preferable not to increase the temperature of the thermolubility of many of these sterilized substances, but even to reduce it. For practical reasons, it is possible to operate at ambient temperature, predominantly, t-z-n. asipri 18 ° to 25 ° C.

The antimicrobial spectrum of pen-xyootic acid is broad in comparison to the other substances and, for this reason, peroxyacetic acid has been used in a number of fields. In the food industry, a positive effect has been found on the disinfection of vegetables (Gree-nspam F., Mc Kellar D .: The - application of peracetic acid germicidal washes to mold control of maitites, Food Technology 5, 3, 95, 1951) and (Greespan FP, Margulles PH: Treatment Plant Tissue, US Patent 2, 512640/1950). that the effect is about 10 minutes at a concentration of 0.01 to 0.001% for vegetative microbes and fungi. The spores are destroyed at a concentration of 0.3 to 0.5%. Peroxyacetic acid is the recommended basic agent for the prevention of liabora-toro-thyroid disease (Wedum AC: Control of Boron Air Bome Infection, Rev. Rev., 25). , 3, 210, 1961). Authors Kline and Hulí (1960) - (Kline LB, Hulí RN: The Virucidal Properties of Peracetic Acid, Amer. J. Clin. Path., 33, 30, 1960) have demonstrated virulence activity against a wide range of viruses in several mi-nutách. The effects of formaldehyde, phenol, sodium hypochlorite, ethanol, various phenol derivatives, quaternary ammonium compounds and peracetic acid were studied in spores and cultures of mold. The effect of peracetic acid was such that it was not possible to establish effective exposure (Krus RH, Green TD et al., Disinfection of Aerosolized Sponges on Laboratory Surfaces, Appl. Microbiol., 11, 11). 436, 1963, II., 12, 155, 1964). Author Gremsam (via Tiohacek, B .: Per-ster, Avicenuim, Prague 1972 p. 26) pointed out the difference between peroxide void and peroxyacetic acid in their brazing of the catalysis of catalase. Hydrogen peroxide is rapidly decomposed but peracetic acid remains intact, but the enzyme itself attacks. K-α-alase is peroxyacetic acid-inactivated. This fact is important for the use of peroxyacetic acid in low concentrations where the peroxide would not be sufficiently effective. In medical use, the disinfection of the body surface is dominant (Právaiora A .: The possibility of disinfecting the hands with peroxyacetic acid, Cs. Epidem., 15, 2, 102, 1966) and sterilization of the instrumental and post-imo-coccidium by immersion in 1 / 2% peracetic acid solution. All microorganisms were destroyed within 2 minutes (P-stata H., Podstata J., Jurak J .: Persta-rilem Sterilization of Instruments, Reports of UEM Prague, 12, 4, 40, 1970). at TBC departments (Svoboda K., Sytářová J .: Utilization of Oxoacetic Acid for Disinfection at the Tulberculosis Department, Czechoslovak Epidem., 19, 4, 206, 1970). peroxyacetic acid to foods allowed. But it is widely used for disinfecting machines and tools in the food industry.

Nowadays, there are a number of well-trained workers who are concerned with the stability of peroxyacetic acid solution (Pazdiora E .: Possibility of using a persterile for focal disintegration, UEM reports, 12, 11, 59, 1970) Furtig, W., Box, A. Ein Beatrag zur Stability! was-seriger verdůnnter Peressigsáurelosungen, 3

Pharmazie 32, 59-60, 1977). For the purifying praise, we can without doubt consider the work of the author of Miicke (Mucke, H .: Untersuchungen uber Einfiusse auf die Zersetzung vanverdunnter Peresisigsure, Fharmazie 32, 613-619, 1977).

Although most authors suggest that the peroxyoxyacetic acid has a low chemical stability, especially in dilute distillation, we can say that the residues of the active bucket (from peroxytoic acid and the hydrogen peroxide contained therein) are detectable for a long time. This conclusion is in agreement with the non-permission of peroxyacetic acid for direct incorporation into foods. A similar situation exists when used for antimicrobial treatment of drinking water.

The above uncertainty that even with the use of very dilute pen-xyotic acid breaks, the amount of active oxygen remaining in the sterilized system remains in the sterilized case and an unpleasant peroxyacetic acid persist in some cases. It is avoided that after the sterilization process the peroxyacetic acid breaks down with a small additive reducing agent. Preferred examples include sulfates, sulfates, dithionites, cysteine, glatathione, sulfur dioxide. It is advantageous to use commercially available sodium sulfite for practical purposes. From the determination of the degree of disruption of the peroxyootonic acid, this can be determined absolutely accurately, but this determination is lengthy in practice, and since we do not need to know the exact amount of residual peracetic acid, it is sufficient for a simple and accurate demonstration of the active oxygen by modifying the reagent. iodine paper (ČSN 687 302).

The proposed and used detection with iodine paper is very sensitive, very precise, very fasting and sufficient in all applications where peroxyethanol is treated.

The antimicrobial treatment spore, and the substance by cold sterilization, is as follows. The appropriate homogenizer is introduced and the substances to be sterilized and perfectly homogenized. Pour the amount of peroxyacetic acid into a homogenizer and homogenize with its contents. Based on solidification with microorganisms and the elongated temperature, we determine the time of action and concentration of peroxyacetic acid. After the sterilization is completed, add the calculated amount of reducing agent, e.g., sodium sulfite and re-homogenize again. After re-opening the homogenizer, the iodine starch paper is detached and the surface of its own sterilized material. In the case of perfect disruption of the peroxyacetic acid, the paper does not change its color, i. not blue. Otherwise, add still small amounts of sodium sulfite. In this case, a product is obtained which, in particular, does not contain any germs and the conventional preservatives are sufficient for its further processing: afco are acidic, its salts and its salts, benzoic acid and its salts, esters of piairahydroxibenzoic acid, (methyl) , proipyl, etc.) etamol, propylene glycol and the like. In the following, the present invention is illustrated by four examples without limiting it to the third one. Example 1 Barium sulphate 55,000 wt.

Water distilled 35,000 wt.

Glycerin 4,000 wt.

Citric acid 0.040 wt.

Saccharin soluble 0.008 wt.

Lemon Aroma 0.100 wt.

Propylene glycol alginate 0.600 wt.

Clhelatone 3 0.055 wt.

Mixture of methyl and propylperaben (4: 1) 0.100 wt.

Potassium sorbate 0.210 wt.

Peracetic acid 0.100 wt.

Sodium sulfite heptahydrate 0.330 1

Water distilled to 100,000 Example 1 - Spoosofo preparation

The suitable homogenizer is water and barium sulphate and homogenised perfectly. Thereafter, the calculated amount of peroxyacetic acid is added and, in the case of the cell, is homogenized with the barium sulphate suspension in water. After the required time has elapsed, the homogenizer is opened and the calculated amount of sodium sulfite, wire, and wire is added and the whole content is completely homogenized. With iodine starch paper, we detect the perfection of acid oxoacetic acid, paper blue. Next, add the prepared pyropylene glycol alginate dispersion in glycerine, in which panabens were hotly dissolved. After homogenization, add citric acid saccharin, Ghelaton 3, potassium sonban, lemon flavor and water residue. All of this has been simulated as a homogeneous suspension of citrus-smelling viscous. Example 2

Aluminum Colloidal Phosphate (Mineral Content 24% w / w, Peracetic AcidWater Distilled Sodium Sulfite Heptahydrate 99.50 wt% 0.05 wt% 0.29 wt% 0.16 wt% Example 2 - Preparation of Preparation

After the separation of the aluminum phosphate of the colloid, we homogenize it into a suitable homogenizer and homogenize it perfectly. Then the calculated amount of peracetic acid is added and the mixture is good homogenized. The peroxyacetic acid was first mixed with distilled water in a ratio of 1: 3. An alumina phosphate blend with peroxyethanol was immediately stored in a well-washed polyethylene container 4 which was sealed. The labeled container is stored in the warehouse. If necessary, the peroxyacetic acid is reduced with the calculated amount of sodium sulfite. For example, the gasterin gel preparation is prepared from the thus prepared semi-finished product, which is the subject of the Czechoslovak invention AO204 670. Example 3

Meat Waste Soy Flakes

Dried SulfateCucor Animal Fat

Vitamins, trace elements, emulsifier

Sodium chloride

Propylene glycol

Sorbitol

Potassium Sorbate Sodium Peracetic Acid Sodium Sulphite heptate hydrate 32.0% wt. 2.5 wt.% 20.0 wt. 1.0 wt. 4.5% w / w 0.6% w / w 2.0% w / w.0.0% w / w 0.3% w / w 0.5% w / w 1.6 wt. Example 3 - Method of preparation Peroxytoic acid is perfectly ground to ground meat waste and completely homogenised. The time required for sterilization is a moderate degree of microbial contamination at which sterilization will be carried out. Upon completion of the sterilization, sodium heptate hydrate is added and the active oxygen is detected by iodine phosphate reagent reagent. Thereafter, the other ingredients are gradually added and the whole is completely homogenised. The final treatment is granulation and the product is filled into cans. It serves as a dog breed for dogs.

Water (demineralized, drinkable, from surface sources to 100,000% w / w Peroxyacetic acid 0.001-1% w / w Sodium sulfite 0.0000131% -0.0131% w / w Example 4 - Prepared in a container fitted with a stirrer to the water The amount of peroxyacetic acid that is thoroughly mixed, and the amount of sterilization required is proportional to the degree of microbial pollution of the starting water and the temperature at which the sterilization will take place, by concentrating the peracetic acid to the degree of microbial contamination and to determine the water. After completion of the sterilization, add sodium sulfite, which is good to dissolve in a small amount of water, and use the reagent iodine starch paper to detect the active oxygen.

For example, in the treatment of drinking water, the amount of peroxyacetic acid was sufficient to be 0.001% sodium bisulfite 0.0000131% to give 0.001869% sodium sulfate and 0.0007897% acetic acid. The acetic acid formed gives a pH value and the amount of sodium sulfate is negligible compared to the sulphate in water commonly found. The importance of treating water from surface sources with peracetic acid and storing it in reservoirs where sulphite-sodium is deprived of active oxygen is of utmost importance with respect to animal production difficulties in our agriculture.

The aforementioned method of decontaminating water with peracetic acid in surface sources is of considerable importance, since the continuous de-contamination is very fast and safe. Only two chemicals and one container are added.

Peracetic acid was discovered in 1900 but was not used until after the Second World War. It is a water-soluble aliphatic acid. Concerning manipulation with it, it is comparable with acetic acid, Zádová.Autor Havel (Havel, S .: Preparation and Properties of Acetic Acid Solution, in: Kyselinaperoxyoctová, Ticháček et al., Zdrav, aktua-lita 369, State. Prague, 1966) followed by physico-chemical constants:

Melting point Boiling pointTension pair

Refractive index nD15nD20

Density U420pK 19.5 ° CPdarograph Concentrations

- 8.2 ° C10 ° C

log p = 8.911-23-23 / T 1.3994 1.3974 1.1037 8.2

E1 / z +1.2 V i / C 7.12 D 1.02 In the purposeful documentation of the oxyoxyacetic acid producers, the decompression of a 1% peracetic acid solution at 25 ° C is given. Time in day: Peroxyacetic acid: Hydrogen peroxide: Active oxygen: 0 1.07 0.3 0.36 3 0.8 0.4 0.35 10 0.6 0.53 0.35 20 0.25 0.75 0.35 30 0.1 0.82 0.35

As can be seen from the table, the content of lactose in the course of decomposition of peracetic acid remains unchanged.

The reaction between the peracetic acid and the reducing agents can be shown on the equation: CH 3 COOOH-f-Na 2 SO 3 - * CH 3 COOH + + Na 2 SO 4

The commercial product (e.g., Persteril) also contains hydrogen peroxide, which is also decomposed by sodium sulfite, but some sodium sulfite is required to increase.

Peroxyacetic acid is one of the properties of explosives. The commercial Persteril product has a safe temperature limit of 65 ° C.

Claims (6)

5 Toxicity (peroxyacetic acid - (Becoo) was 100 ml of 0.01% solution of insensitive effect on white mice and guinea pigs. Data in Persteril (Tidhechek, B., 1972, Avicenum Prague) describe inhalation toxicity to white rats , starting with a concentration of 7.2 mg per 100 liters of air, corresponding to the spraying of about 1% of the solution, as well (the fact that none of the laboratory workers, where peracetic acid was working, was described in the book) .to this intoxication or mountain disease - OBJECT A method of antimicrobial treatment with peroxyacid steirilization, wherein after treatment the substances contain active oxygen residues and are sterile, characterized in that the treated substance is homogenized with the peroxyacid and its residues are removed from the system by disruption by addition of a reducing agent until negative result in the detection of active oxygen. 2. The method of claim 1 wherein the peroxyacid is peroxyacetic acid. 3. Method according to items 1 and 2 characterized by respiratory tract. Acute per os toxicity of white rats is 1¾ = 210 mg / 1 kg. The author Veger (Veger, J., Peraral Toxicity of Pensteril, Voj. Health, Sheets, 36, 3, 114, 1967) found in a test run on rats that the data needed to disinfect the drinking water (ie, 1 mg / 1 liter for a 30 minute expo) or 10 mg per liter at 10 min exposure) are harmless. However, direct food incorporation is not recommended. DISCLOSURE OF THE INVENTION in that peracetic acid is used at a concentration of 0.001% to 1% by weight. 4. A method according to claim 1, wherein the temperature of the sterilized system is from 0 ° C to 50 ° C. 5. The method of clause 1, 2, 3, and 4 is characterized by the fact that the residual active oxygen is detected by iodotron paper. 6. The method of clauses 1, 2, 3, 4 and 5 wherein the reducing agents are sulphites, sulphites, dithionites, cysteine, glutathione, sulfur dioxide, preferably alkali metal sulphite.