CS215779B1 - Process for preparing antibiotic and other biosynthetic preparations - Google Patents

Abstract

The invention relates to the preparation of antibiotic and other biosynthetic preparations, in particular antibiotics, by cultivating production microorganisms in liquid live soil of usual composition and under usual conditions, with the addition of so-called ergot oil as an antifoam and/or a partial source of assimilable carbon.

Description

The invention relates to a process for the preparation of antibiotic and other biosynthetic preparations, in particular antibiotics, by culturing production microorganisms in a liquid, Jewish soil containing sources of assimilable carbon nitrogen and mineral living salts, optionally precursors, silencers and biofactors under aerobic conditions.

As is known, in biosynthetic processes, during the cultivation of the production microorganism, depending on the composition of the medium and the intensity of mixing and aeration, foam must be removed, either by mechanical, physicochemical or chemical means, usually by the addition of so-called antifoams. Chemical defoamers are divided into natural and synthetic defoamers and their properties are described in detail by G.L. Solomons / Process Biochem. 2,

10, 1967, p 47. Of the natural chemical antifoams, the most commonly used are vegetable oils and animal fats, which are also partly used as carbon sources by the producing microorganisms. Of the vegetable oils, soybean, corn, peanut, cotton, barley germ oil, coconut oil, etc. are commonly used, and animal fat is lard, bone fat, shea oil, etc.

In an effort to replace soybean oil; An important part of humorous nutrition, another effective antifoam, has been sought and found a suitable source in so-called ergot oil, which is a waste product of ergot alkaloid processing. The ergot oil is not a chemical individual, it is a mixture of triglycerins of higher fatty acids with ergosterol and other plant sterols and triterpenic alcohols whose composition may vary according to the producer's cultivation conditions.

Using this new effective antifoam, a process for the preparation of antibiotic and other biosynthetic preparations, in particular antibiotics, according to the invention has been developed, which comprises cultivating with a single or repeated addition of so-called ergot oil, a mixture of triglycerides of higher fatty acids with ergosterol. plant sterols and triterpenic alcohols, falling off in the treatment of ergot alkaloids as antifoam and / or a partial source of assimilable carbon, in an amount of 0.1-10.0% by volume based on the volume of living soil.

The ergot oil is freed from the extraction residue by thorough evaporation prior to use as antifoam so that handling and application to the fermentation or culture media is permissible from a safety point of view.

In cultivation, ergot oil is added either at the beginning of the process or additionally during the process, if it is necessary to antifoam. In addition to this effect, ergot oil is also used as a partial source of assimilable carbon, with a beneficial effect on the production of the biosynthetic preparation, which is 5-20% higher than the control.

The process according to the invention thus enables the recovery of the waste product and the saving of soybean oil usable for human nutrition.

Further details of the process according to the invention can be seen from the following examples.

Example 1

To the culture medium for biosynthesis of an antifungal antibiotic by the Oude2 production strain

215 779 mansiella mucida was added to the seed tanks in addition to 2 to 10 wt.% Of glucose, 1 to 2 wt. % peptone, 2 to 3 wt. % corn extract, 0.2 to 1.0 wt. The crystalline magnesium sulphate adds an ergot oil in an amount of 0.05 to 0.2% by volume. % glucose, 1-2 wt. % peptone, 2-4 wt. molasses, in an amount of 0.1 to 0.3% by volume. Compared to control under the same conditions, using soybean oil as an antifoam, the increase in antifungal antibiotic production is on average 5% of theory.

Example 2

In addition to 0.5 to 2.0 wt. % sucrose, 0.2 to 2.0 wt. % urea, 4 to 7 wt. The corn extract adds ergot oil in an amount of 0.05 to 2.0 7 vol. equipment after the signal photocells located at the air outlet. Compared to control, the increase in oxytetracycline production is 18-20% of theory.

Example 3

In the culture medium for submerged ergot vaccine preparation, 3 to 5 wt. % sorbitol, 2-4 wt. % corn extract, 1 to 3 wt. citric acid adds 0.1 to 0.3% by volume of ergot oil, also as needed during cultivation. Compared to control, the increase in production is on average 5% of theory.

Example 4

In a culture medium for fungicidin biosynthesis by the production strain Streptomyces nourei, 6 to 10 wt. % fructose syrup, 3 to 5 wt. 0.5 to 2.0 wt. of corn extracts, 0.5-2.0% by weight, of ammonium nitrate add 0.1% by volume of ergot oil.

Example 5

% Of the culture medium for tylosin biosynthesis with Streptomyces fradiae. molasses, 0.2 to 0.8 wt. % ammonium phosphate, 2 to 5 wt. % fish meal and 1 to 3 wt. The corn extract adds 1 to 3% by volume of ergot oil. Compared to control, the increase in tylosin production averages 10% of theory.

Example 6

In the culture medium for penicillin G biosynthesis, the production name Penicillinum chrysogenum with 3 wt. % cotton flour extract, 0.5 wt. % sucrose and 2 wt. 1 to 4% by volume of ergot oil are added to the corn extract. Compared to control, the increase in penicillin production averages 4% of theory.

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Example 7

In a culture medium for chlortetracycline biosynthesis, a production strain of Streptomyces aureofaciens with 3 wt. % starch, 1.5 wt. % sucrose, 3 wt. % soya flour and 0.5 wt. 1 to 10% by volume of ergot oil are added to the corn extract. Compared to the control, an increase in chlorotetracycline production of an average of 3% of theory was achieved.

Claims (1)

A method for the preparation of non-antibiotic and other biosynthetic preparations, in particular antibiotics, by culturing production microorganisms in a liquid living medium containing assimilable carbon and nitrogen sources and mineral living salts or precursors, stimulators and biofactors under aerobic conditions, characterized in that the cultivation is carried out for single or repeated additions of so-called ergot oil consisting of a mixture of triglycerides of higher fatty acids with ergosterol, plant sterols and triterpenic alcohols, falling off during the processing of ergot into alkaloids as antifoam & / or a partial source of assimilable carbon, in an amount of 0.1 to 10.0 i vol based on live soil volume.