CS211893B1 - Method of immobilization of the microbial cells in the silicate microporous carrier with defined size of the pores - Google Patents

Description

SUMMARY OF THE INVENTION The present invention provides a method for immobilizing microbial beads into a silicate miciroporous carrier having a defined pore size, comparable to the diameter of the microbial culture.

Heretofore, a porous glass carrier having the desired pore size from an economically disadvantageous import, or a ceramic material manufactured for other purposes, such as tetol, has been used to bind biological material with catalytic activity. The cells limohilized to these carriers can then be used as fillers for heterogeneous biochemical constructors.

According to the present invention, the isibosib immobilization of miciroibular cells into a silicate microporous carrier having a defined pore size comparable to that of a butobutton culture of microorganisms, and that for bacteria of the order of 1 μτη and for yeast 10 μτη, two parts of the suspension of cells for immobilization were added, the carrier being treated by mixing, suctioning and grinding 2 parts by weight (calcium oxide with 8 parts by weight of silicate volcanic rock - dacitic or rock) and a particle size of 90 µm to which 4 to 4 6 parts by weight of water, further heating the mixture for 8 to 9 hours at 190 ° C, crush the icompact block of microporite and stratify on ice and float, and use a 0.5 μια particle size silicate microparticle (used).the cells are mixed, washed with water or buffer from the newly bound cells, decatotoed and used for heterogeneous biochemical irradiation.

The advantages of the present solution are that prior immobilization of the desires does not require prior chemical surface treatment of the carrier or cells, and the desired pore size can be controlled by varying the amount of water added. Cells itmo-mobilized in this manner do not elute from the carrier and exacerbate metabolic activity to facilitate a given bio-chemical reaction. The carrier with immobilized lymphocytes can be used as a filler in a pellet-flowing phenolone heterogeneous biochemical reactor or in a mixed heterogeneous reactor.

The subject matter of the invention is described in the following examples without reference to Siba.

Example 1 and 1

Mix 2 parts by weight of calcium oxide with 8 parts by weight or tartrate, ground to a particle size of 9 .mu.m. After the addition of 4.8 wt. The thus obtained comipalctic block of milkroporite was crushed and fractionated on sieves and floats to prepare a fraction with a grain size of 0.5. mm. After drying, the material was used on too much cell pellet.

Example 2

A culture of benzene-quenching bacteria and a culture capable of quenching cyanobenzoic acid were isolated from the accumulated culture by over-centrifuging the refinery wastewater sample with a culture medium on a mineral soil with ammonium chloride as the nitrogen source and said organic compounds as the source. Bars of 1.0 x 1.5 μτη size were obtained. 1 weight by weight of the microporous carrier prepared according to the procedure of Example 1 was mixed with 2 weight by weight of cell suspension. Excess culture and unstable cells were removed by washing with β-µmr and decantation.

The activity of Vlazanla was monitored from the difference in turbidity of the suspension before ivlazing and after binding to the carrier. For this purpose, a culture labeled with radonucleotide was prepared by culturing on stages with sodium acetate - C ¹⁴ or glucose - C ^{14 addition} . The amount of cells bound to the carrier micropores was judged from the amount of radiolabelled activity.

The results are shown in Table 1: For comparison, the efficacy of cell-bound d-6 microporte prepared by the process of Example 1 is given by the data on the cell-binding ability of other inorganic materials and the binding efficacy of microorganisms as microorganisms as microorganisms. per carrier of the total number of cells in suspension. before adding the carrier.

Table 1

.Keramický carrier ° / o glazed microorganisms on a carrier bacteria yeast before for- wash after washing before for- wash jpoipire- wash fireclay. 43.4 10.2  8.2 1.3 corundum 45.1 21.7 6.4 0.6 alpozíd 47.8 16.5 26.4 2.0 marine Pleis-ok 15.3 2.7 20.6 0.01 sandy earth 58.3 13.7 22.0 1.3 mífcro- polt 75.1. 75.0 38.6 1.7

EXAMPLE 1 a d 3

The SuS-penzla cells deposited on the microporous material prepared according to Example 2 were incubated in ferm-en-fore on mineral pQde with 0.2% benzoic acid as a substrate and the oxygen consumption was recorded by Clark's polarogirafic electrode, Bacterial culture. thio-sensitized to mliproiporiite exhibited a response activity of 160 mg Oz / hr. per kg of immobilized preparation.

Claims (2)

1. S-shift of the microbial cell immobilization into a silicate microporous carrier of defined pore size, characterized in that, per 1 part by weight of a microporous carrier having a grain size of 0.5 mm, prepared by mixing 2 parts by weight of calcium oxide and 8 parts by weight. - daclt tuft ground to a particle size of 90 µm and 4 to 6 parts by weight of water, OF THE INVENTION at a temperature of 190 ° C for 8 to 9 hours, -breaked and fractionated with separation of the appropriate fraction mechanically and by float, were added 2-weight-sized bacteria with a diameter of the order of 1 μία or a quasiinieik with an order of magnitude of 10 tum; Further, glazed bacteria or yeast are removed from the carrier by washing with water or a putter and a detachanthate.