CS211166B1 - Closure medium for cytological microscopy technique - Google Patents

Abstract

The invention belongs to the field of histological microscopic technique. It relates to a mounting medium for cytological microscopic technique, which allows to simplify and at least accelerate the time for mounting the preparation. For this purpose, alkylphenols with a number of carbon atoms in the alkyl of 8 to 12, preferably nonylphenol, are used.

Description

1 211166

BACKGROUND OF THE INVENTION The present invention relates to a closure medium for a cytological microscope technique which makes it easier and more expedient to close the closure time.

Up to now, water-miscible media such as gelatin medium, lactophenol, colorate medium, Apathy syrup, Jones, Downos or Andean sealing medium and others are used to prepare preparations that need to be evaluated in a short time. They are mixtures of chemical substances with some toxic components, such as arsenic oxide, acid-capric or phenol. When used in the course of microscopy, they are rapidly removed by the microscope, thus destroying the quality microscopic image after only a few hours. and then supersaturate with a substance that dissolves the sealing medium. Organic solvents such as xylene, benzene, n-butanol, chloroform, carbon tetrachloride, which rapidly evaporate and deteriorate the working environment, are used for this preparation-prolonging preparation time.

The above mentioned drawbacks have been eliminated in the invention of the closure medium by a precytological microscopic technique which is based on a monoalkylphenol having a carbon number of 8-12, preferably a nonylphenol.

A closure medium of this type can be used in microscopic techniques to prepare sustained-release preparations by rapid depressurization, with the drainage operation and the use of harmful solvents being substantially eliminated. . Another important feature is that after documenting the preparation, it is possible to separate the backing and cover slip from it without using a solvent such as xylene and using it again after washing. The preparation can be stored as a permanent preparation for a long period of time (up to 12 months), without the use of a border technique and further preservation. The closure medium of the invention does not evaporate, has the desired refractive index, does not adversely affect the microscope optics.

The subject matter of the invention is used in the following examples, without being bound by the following examples

The biological preparation, such as cell cultures or cells in vivo, was fixed, macerated, by a conventional method. The staining was performed as follows. by a rapid method, and toacetocarmine, acetoorcein, acetonigroin, or other dye, with color dyeing. After the dye was kneaded at an oblique position, a nonylphenol capping medium was immediately dropped onto the stained cells in the monolayer. The slide was covered with a coverslip, which was pressed with a preparatory needle to remove excess dye and clarify the image. The instantaneous specimen had an observation object brightened with the desired refractive index and evaluated after retention for a long time (12 months) at observation at regular time intervals, the specimen image unchanged compared to immediate evaluation. Over time there was no deterioration of the preparation or a decrease in the quality of the image observed, nor the evaporation of the sealing medium used. Example 2

The biological prepalate was processed after staining as described in Example 1. Staining was carried out with the coloring mixtures used in the preparation. permanent preparations such as Feulgen staining. A closing medium, nonylphenol, was used. The effects of the closing medium are also as in Example 1. *

Claims (3)

OBJECT OF THE INVENTION The use of alkylphenols 8 by the number of carbons in the alkyl of 8 to 12, preferably nonylphenol, as a locking medium for the cytological microscopy technique.