CS207104B1 - Method of production of the protein biomass from the hydrolyzators of the beech-tree bark - Google Patents

Description

CZECHOSLOVAKCASOCIALISTREPUBLIC (19)

DESCRIPTION OF THE INVENTION

TO COPYRIGHT CERTIFICATE (61) (23) Show Priority (22) Enrolled 10 12 79 (21) PV 8575-79 207 104 (Π) (Bl) (51) Int. Cl.3 A 23 K 1/12

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AND DISCOVERIES (40) Published 15 09 80 (45) Published 01 11 83

Author of the invention KRAMÁR ALOIZ ing. CSc., EBRINGEROVÁ ANNA Ing. CSc., KARÁCSONYI STEFAN ing. CSc., BRATISLAVA, BÁRTOVÁ MARTA ing., BARTA Jlfií ing. A KRUMPHANZL VLADIMÍR prof. DrSc., PRAGUE * 1 (54) Method of protein biomass production from hydrolysates of beechwood 1

BACKGROUND OF THE INVENTION The present invention relates to a method for producing proteinaceous biomass from beech hydrolysates to microcapsules.

It is known that about 10% of the horseradish (F. Kozmál: Paper and Pulp 18, 2. 9, 175/1963) is eliminated from the tree species during its industrial processing. hThe point of industrial exploitation of bole is insignificant. At present, however, it is subject to considerable attention, since in the timber production process, wood-logging is predominantly carried out in the timber-processing plants where wood waste is concentrated, which requires its efficient disposal with effective and practical use for many purposes in our national economy ( K. Kurschner: Drevárskyvýskům 2, No. 1, page 5 (1957).

In general, the chemical composition of the crack varies considerably from the chemical composition of the wood. The bark has a high content of ash, lignin, extractive substances and pectic substances, but a lesser total polysaccharide fraction (N.I. Nikitin: Wood Chemistry, page 338, SNTL, Prague1956). For example, the analysis of the beechwood was found to contain about 20% cellulase and 26% non-celluloid polysaccharides (A. Kramář, L. Zákutná, A. Ebringer: Paper and Cellulose 32.11, V 57 (1977). The polysaccharide component of the wood species is in comparison with the cellulose-hydrocarbonate (AP Zakoščikov: Gidrolizn. p. 1, 5 (1952)), depending on the reaction conditions of the hydrolysis, providing hydrolysates containing a mixture of e-carbohydrates in the range of 3, 9 to 18.5%, referring to absolutely thick beech horsetail, with a total of about 104% of the pentaches embedded therein, of about 62%, of hexoses of 8% and of aldouronic acid of 30%. some of the wastewater g of cellulose, e.g., sulphite leachate, which has a high kyeloet and high temperature. The compositions of the present invention provide an increased amount of utilizable substances. The atrium is enhanced in the practice of the production of biomaoy productivity of fermentation units and separates.

Hydrolyzates of the nostril represent a potential substrate for the production of microbial proteins, which represent an important component of the feed mix. It turned out to be a good producer! bio-masses are selected strains of some yeast microorganisms, namely Candide arborea MDUS-35, which is deposited in the collection of Microbiological Institute of the Czechoslovak Academy of Sciences, Prague, Candide utilie vararborea C-164, Cryptocooeue albidus var diffluens C-207 and their mix, which are stored collection of the Department of Fermentation Chemistry and Technology at the Institute of Chemical Technology, Prague.

SUMMARY OF THE INVENTION The subject of the present invention is that the hydrolysis of ea is carried out with dilute acid solutions, preferably 1 to 2% sulfuric acid solution, optionally sulphite leachate pH 1.2 to 2.5, and 500 to 1500 mg of free sulfur dioxide; 110 ° C for 4 to 6 hours, and the pH of the obtained hytfrolysate e adjusted to pH 4.2 to 5.5 with potassium hydroxide and ammonia, and with phosphorus and magnesium exidative fermentation with Candide utilized var arborea C-164, Candide arborea MDU S- 35, Cryptocooeue albidusvar diffluens C-207, or mixtures thereof, in a quantity of 1.0 to 1.5 g of biomass solids of 1 l hydrolyzate for 24 to 48 hours. Oxidative fermentation process e and in addition to one to three stages.

Under these conditions, an abeolute biomass increment of 4 to 5.8 g / l is obtained, corresponding to 4.35 to 6.3 kg per 100 kg of absolute dry kCra. The utilization of each of the aridic components of the hydrolysis was high, indicating a decrease in the content of reducing beads from 24.5 g / l and 20 g / l to 4.5 g / l to 4.35 g / l. The biomass obtained showed a good nutritional value from the chemical point of view and the crude protein content in the washed biomass was around 55 $ 8 with a digestibility coefficient of 85%. The content of the popols was around 7% of ribonucleic acids + desoxyribonucleic acids was about 6%. that: the biomass production by yeast-microorganism represents one of the cissts used by the wastes of beech, which is produced by the accepted mechanical and chemical treatment, the preparation of the hydrolyzate from the beechwood is technologically undemanding, dilute kyeelins can be used for hydrolysis, eventually the acidic waste water, e.g. as the starting material for the hydrolyzate preparation may be used the bush of other deciduous trees, possibly coniferous trees, selected fermented microorganisms Candide arborea MDU S-35, Candide utilie var arborea C-164 207 104

Cryptococcus albidua var diffluene C-207 and mixtures thereof are distinguished by their good utilization of sugar components of the hydrolyzate, the biomaeae produced and the good nutritional value.

For the purpose of illustration, the following examples are not intended to limit the scope of the present invention. Example 1

Beech co-hydrolyzate was prepared under catalytic action of 1% sulfuric acid at 100 ° C for 4 hours. 2.4 kg of ammonium sulfate, 0.4%, are added to 100 1 of the hydrolyzate containing 20 g / l of reducing agents. kg of ammonium phosphate, 0.21 kg of potassium hydroxide, 0.1? magnesium chloride hexahydrate, and the pH is adjusted to 5.0 with 25% ammonia water. After cooling to 32 ° C, the inoculum of the propagated Candida arborea MDU S-35 culture is added in an amount corresponding to 1.5 g of absolutely dry biomass per liter of medium, i.e. 1.5 kg of absolutely dry biomass. Poinoculation is fermented by blowing air at 500 L / min. After 24 hours fermentation, the fermenter contents are centrifuged and the fermentation milk is washed twice with water on the separator. Drying in a spray dryer yields 6.68 kg of 90% dry product, i.e. 6.08 kg of absolutely dry biomass. This corresponds to an increase of 4.58 kg of absolutely dry biomasys containing 55% crude protein, 7% ash, 6% ribonucleic acids ♦ deoxyribonucleic acids, with a coefficient of vitality of 0.85 · Example 2

The buffalo hydrolyzate was prepared at a catalytic effect of 2% sulfuric acid at 100 ° C for 4 hours. For 1000 l of hydrolyzate containing 24.5 g / l of reducing agents, add: 3.0 kg of ammonium sulphate, 0.56 kg of ammonium phosphate, 0.27 kg of potassium hydroxide, 0.18 kg of magnesium chloride hexahydrate and adjust the pH to 35% ammonia water to 5.0. After cooling to 34 [deg.] C., the cultured cultured Candida utilis var arborea C-164 culture was added to an amount corresponding to 1 g of biomass dry matter per 1 l of hydrolyzate, i.e. 1 kg of dry matter. After inoculation, 500 ml / min was charged at the indicated temperature and pH for 18 hours. After completion of the fermentation, the fermenter contents are centrifuged and the yeast milk obtained is processed in Example 1. 7.48 kg of 91% dry matter product is obtained, i.e. 6.8 kg of absolutely dry biomass. This corresponds to an increase of 5.8 kg of absolutely dry biomass containing 55% crude protein, 7% of

Claims (2)

207,104 poles, 6% ribonucleic acids + deoxyribonucleic acids, with a tele-coefficient of 0.65. EXAMPLE 3 A beech stool hydrolyzate was prepared under the catalytic effect of a parent calcium bi-sulfite leachate with a pH of 1.5 and containing 1000 mg of free sulfur dioxide and 38 g / l of reducing agents at 110 DEG C. for 4 hours. To 1000 l of hydrolyzate and 62 g / l of reducing agents, add: 10 kg of ammonium sulphate, 3 kg of ammonium phosphate, 1.45 kg of potassium hydroxide, 1 kg of magnesium chloride hexahydrate and adjust the pH to 25 with 25% ammonia water. . After cooling to 34 ° C, the cultured cultured Cryptococcus albidus var diffluens C-207 culture was added in an amount of 1.2 g of biomass dry matter per 1 l of hydrolyzate, i.e. 1.2 kg of yeast solids. After inoculation, air was blown for one hour at the indicated temperature and pH for 16 hours. At the end of the fermentation, the fermenter content is processed as in Example 1. 28.25 kg of a 92% dry matter product, i.e. 26 kg of absolutely dry biomass, are obtained. This corresponds to an increase of 24.8 kg yeast dry matter with a crude protein content of 52%, an ash of 9%, 6.4% of ribonucleic acids + deoxyribonucleic acids, with a coefficient of spending 0.83% of PBEDlBETH. 1. A method for producing protein biomass from beech herring hydrolysates, characterized in that the hydrolysis is carried out with dilute acid solutions, preferably 1 to 2% sulfuric acid solution, optionally with sulfite leachate of pH 1.2 to 2.5 and 500 to 1500 mg of free sulfur dioxide, at a temperature of 100 to 110 ° C for 4 to 6 hours, and the obtained hydrolyzate is adjusted to pH 4.2 to 5.5 with potassium hydroxide and ammonia, fed phosphorus and magnesium nitrogen and fermented by cultures Candida utilis vararborea C-164, Candida arborea MDU S-35 »Cryptococcus albidus var diffluens C-207 or mixtures thereof amounting to 1.0 to 1.5 g of biomass dry matter per liter of hydrolyzate in the form of 24 to 48 hours. 2. Process according to claim 1, characterized in that the oxidative fermentation process is carried out continuously in one to three steps. Printed by Moravské tiskařské závody, provoz 12, Leninova 21, Olomouc Price: 2,40 Kčs