CS205643B1 - Method of inactivation of the microorgasms - Google Patents
Method of inactivation of the microorgasms Download PDFInfo
- Publication number
- CS205643B1 CS205643B1 CS890578A CS890578A CS205643B1 CS 205643 B1 CS205643 B1 CS 205643B1 CS 890578 A CS890578 A CS 890578A CS 890578 A CS890578 A CS 890578A CS 205643 B1 CS205643 B1 CS 205643B1
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- dimethyl
- oxide
- microorganisms
- cells
- methyldodecylamine
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 16
- 230000002779 inactivation Effects 0.000 title description 11
- 244000005700 microbiome Species 0.000 claims description 24
- LSTLKVDCBYTHPD-UHFFFAOYSA-N n,n-dimethyltridecan-2-amine oxide Chemical compound CCCCCCCCCCCC(C)[N+](C)(C)[O-] LSTLKVDCBYTHPD-UHFFFAOYSA-N 0.000 claims description 16
- 238000004659 sterilization and disinfection Methods 0.000 claims description 11
- 230000000415 inactivating effect Effects 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 239000000945 filler Substances 0.000 claims description 2
- 238000004806 packaging method and process Methods 0.000 claims description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 1
- 229910019142 PO4 Inorganic materials 0.000 claims 1
- 229940071106 ethylenediaminetetraacetate Drugs 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 239000010452 phosphate Substances 0.000 claims 1
- 150000001412 amines Chemical class 0.000 description 8
- 230000003115 biocidal effect Effects 0.000 description 7
- 239000000126 substance Substances 0.000 description 6
- 239000000645 desinfectant Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000004094 surface-active agent Substances 0.000 description 5
- 235000013305 food Nutrition 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 241000235070 Saccharomyces Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 235000013365 dairy product Nutrition 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000009331 sowing Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000002599 biostatic effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 229910052736 halogen Inorganic materials 0.000 description 2
- 150000002367 halogens Chemical class 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 230000003381 solubilizing effect Effects 0.000 description 2
- 241000228245 Aspergillus niger Species 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000123650 Botrytis cinerea Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000192130 Leuconostoc mesenteroides Species 0.000 description 1
- 241001149951 Mucor mucedo Species 0.000 description 1
- 241000235526 Mucor racemosus Species 0.000 description 1
- 241000221961 Neurospora crassa Species 0.000 description 1
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 description 1
- 241001507683 Penicillium aurantiogriseum Species 0.000 description 1
- 241000228145 Penicillium brevicompactum Species 0.000 description 1
- 240000000064 Penicillium roqueforti Species 0.000 description 1
- 235000002233 Penicillium roqueforti Nutrition 0.000 description 1
- 241000588767 Proteus vulgaris Species 0.000 description 1
- 240000005384 Rhizopus oryzae Species 0.000 description 1
- 235000013752 Rhizopus oryzae Nutrition 0.000 description 1
- 241000235546 Rhizopus stolonifer Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- -1 aniline oxide Chemical compound 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 239000003945 anionic surfactant Substances 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 238000009924 canning Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 239000003093 cationic surfactant Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 230000003641 microbiacidal effect Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000017066 negative regulation of growth Effects 0.000 description 1
- 231100000344 non-irritating Toxicity 0.000 description 1
- 239000012454 non-polar solvent Substances 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 150000002902 organometallic compounds Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229940098377 penicillium brevicompactum Drugs 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000003495 polar organic solvent Substances 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 229940007042 proteus vulgaris Drugs 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
Predmetom vynálezu je spflsob inaktivácie mikroorganizmov v sanitačných a dezinfekčných procesoch N,N-dimetyl-l-metyldodecylamínoxidom za podmienok jeho optimálnej biocídnej aktivity.It is an object of the present invention to provide a method for inactivating microorganisms in sanitation and disinfection processes with N, N-dimethyl-1-methyldodecylamine oxide under conditions of optimal biocidal activity.
V dezinfekčných procesoch inaktivácia mikroorganizmov sa dosahuje použitím rSznych fyzikálních alebo chemických prostriedkov. PodTa chemickej povahy možno antimikróbne látky používané v dezinfekci! rozdělit do viacerých skupin. Tieto skupiny tvoria látky uvoTnujúce kyslík, halogány a látky uvolňujúce halogén, kyseliny a lúhy, tenzidy, organokovové zlúčeniny a rozličné organické zlúčeniny typu fenolov, alkoholov, aldehydov, éterov a farbív.In disinfection processes, the inactivation of microorganisms is achieved by using various physical or chemical means. Depending on the chemical nature, antimicrobial substances used in disinfection may be used! divided into multiple groups. These groups include oxygen releasing agents, halogens and halogen releasing agents, acids and lyes, surfactants, organometallic compounds and various organic compounds such as phenols, alcohols, aldehydes, ethers and colorants.
V technologickéj praxi, obzvlášť pri sanitácii v potravinárskom priemysle sa na chemické dezinfekčně prostriedky inaktivujúce nežiadúce mikroorganizmy kladů mimoriadne náročné požiadavky. Mnohé z doteraz používaných prostriedkov sú však korozívne, toxické, odolávajú biologickéj degradácii, čím znehodnocujú toky a životné prostredie, sú nerozpustné v nepolárných rozpúšťadlách, sú inkompatibilné s tenzidmi a podobné.In technological practice, especially in sanitation in the food industry, chemical disinfectants inactivating unwanted microorganisms are subject to extremely demanding requirements. However, many of the compositions used so far are corrosive, toxic, resist biological degradation, thereby degrading fluxes and the environment, insoluble in nonpolar solvents, incompatible with surfactants, and the like.
Tieto nevýhody odstraňuje rlešeníe podTa vynálezu, ktoré sa týká spSsobu inaktivácie mikroorganizmov Ν,Ν-dimetyl-l-metyldodecylamíhoxidom, ktorého schopnost inaktivovat /ničit, usmrcovať/ mikroorganizmy zatial* popísaná nebola. VzhTadom na závislost biocídneho efektu biologicky aktívnych látok na vonkajších faktoroch /teplota, čas pflsobenfa, koncentráoia, pH a pod./, vynález Specifikuje aj podmlenky optimálnej biocídnej aktivity N,N-dimetyl-l-metyldodecylamínoxidu, ktoré sú podobné ako u iných biologicky aktívnych látok používanýchThese disadvantages are overcome by the present invention which relates to a method for inactivating microorganisms with Ν, dim-dimethyl-1-methyldodecylamine oxide whose ability to inactivate / kill, kill / microorganisms has not been described. Given the dependence of the biocidal effect of biologically active substances on external factors (temperature, adaptation time, concentration, pH, etc.), the invention also specifies conditions for optimal biocidal activity of N, N-dimethyl-1-methyldodecylamine oxide similar to other biologically active compounds. substances used
205 6*3205 6 * 3
20S 643 v aanftačných a dezinfekčných procesoch z hlediska efektívnej dezinfekcie nepostrádateTné.20S 643 in sanitation and disinfection processes is essential for effective disinfection.
Účinnou látkou, ktorá spdsobuje 1'naktivéciu mikroorganizmov je podl’a vynálezu N,N~dimetyl-l-metyldodecylamínoxid. Je to biela, krystalické, hygroskopická zlúčenina a teplotou topanfa 91 až 93 °ύ, o molekulovej hmotnosti 243,44. Je rozpustná vo vodě i v polárných organických rozpúšťaďlách /etanol, metanol, chloroform, dichlórmetan a pod./. Je kompatibilně a katiónaktívnymi, aniónaktívnymi i neionogénnymi tenzidmi. Mé povrchovo aktivně a solubilizačné vlastnosti a je zaraditelná k detergentom neiSnového typu.The active ingredient which causes the inactivation of the microorganisms is according to the invention N, N-dimethyl-1-methyldodecylamine oxide. It is a white, crystalline, hygroscopic compound having a melting point of 91-93 ° ύ, a molecular weight of 243.44. It is soluble in water and in polar organic solvents (ethanol, methanol, chloroform, dichloromethane and the like). It is compatible and cationic, anionic and nonionic surfactants. It has surfactant and solubilizing properties and is classifiable as nonionic detergents.
Toxicita Ν,Ν-dimatyl-l-metyldodeeylamínoxidu Je nízká, LDjjq při podaní par os činí 960 mg/kg. V koncentrácii 0,73 % aplikovaná na kožu je ešte nedráždivá.Toxicity of Ν, Ν-dimatyl-1-methyldodeeylamine oxide is low, the LD50 when administered with vapor is 960 mg / kg. At a concentration of 0.73% applied to the skin is still non-irritating.
N,N-dimetyl-l-metyldodecylamfnoxid inhibuje raat vegetatívnych bunfek prokaryotických a eukaryotických mikroorganizmov. Je vysoko účinný proti baktériám, kvasinkám i vláknitý^ hubám. Qrampozitívne baktérie sú citlivejšie k účinku amfnoxidu ako gramnegatívne. Pdsobí i na aparulujúee mikroorganizmy. Pri vyšších koncentráciách inhibuje germináciu apór, pri nižgích dovolí ešte sporem vyklíčit, ale inhibuje už ich rast. Tab. 1 a tab. 2 udávájú zóny inhibícle rastu prokaryotických a eukaryotických mikroorganizmov na povrchu agerových médií.N, N-dimethyl-1-methyldodecylamphnoxide inhibits vegetative cells of prokaryotic and eukaryotic microorganisms. It is highly effective against bacteria, yeast and filamentous fungi. Q-positive bacteria are more sensitive to the effect of amphnoxide than gram-negative. It also affects microorganisms. At higher concentrations it inhibits germination of apores, at lower levels it still permits germination, but inhibits their growth. Tab. 1 and tab. 2 show zones of growth inhibition of prokaryotic and eukaryotic microorganisms on the surface of ager media.
V tekutých médiách 0,005 % Ν,Ν-dimetyl-l-metyldodecylamínoxid inhiboval rast všetkých testovaných mikroorganizmov /tab. 1 a tab. 2/, včítane kvaainiek Saccharomyces cerevieiae a Candida albicans.In liquid media, 0.005% Ν, Ν-dimethyl-1-methyldodecylamine oxide inhibited the growth of all tested microorganisms / tab. 1 and tab. 2), including Saccharomyces cerevieiae and Candida albicans.
Spdsob účinku Ν,Ν-dimetyl-l-matyldodecylamínoxidu spočívá v dezorganizácií membránových štruktúr mikroorganizmov. Amínoxid má výrazný cytolytický účinok, ktorý je zrejme zodpovědný za jeho biocídnu aktivitu.The mode of action of Ν, Ν-dimethyl-1-methyldodecylamine oxide consists in the disorganization of membrane structures of microorganisms. The amine oxide has a potent cytolytic effect, which is apparently responsible for its biocidal activity.
Ν,Ν-dimetyl-l-metyldodecylamínoxfd nie je mutagénny. Neindukoval tvorbu cytoplazmatických reapiračne deficitných mutantov u kvaainiek Saccharomyces cerevieiae ani revarziu k prototrofu u baktérií Salmonella typhimurium TA 100.Ν, Ν-dimethyl-1-methyldodecylaminoxfd is not mutagenic. It did not induce the production of cytoplasmic respiratory deficient mutants in Saccharomyces cerevieiae cvaaines, nor reversal to prototroph in Salmonella typhimurium TA 100.
Od biostatického je kvalitativně odlišný biocídny účinok N,N-dimetyl-l-metyldodecylamínoxidu. N,N-dimetyl-l-metyldodecylamínexid efektívne inaktivuje vegetativně formy patogénnych a saprofýtických mikroorganizmov. Minimálně mikrobicídne koncentrácia N,N-dimetyl-l-metyldodecylamínoxldu aú relativné vyššie ako odpovedajúce biostatické koncentrácia, obe však ešte ležia v premícelárnych koncentráciách amínoxidu. Biocldna aktivita Ν,Ν-dimatyl-l-metyldodecylamínoxidu stúpa s koncentráciou amínoxidu s teplotou a časom expozície.The biocidal effect of N, N-dimethyl-1-methyldodecylamine oxide is qualitatively different from the biostatic one. N, N-dimethyl-1-methyldodecylamineexide effectively inactivates vegetatively forms of pathogenic and saprophytic microorganisms. At least the microbicidal concentrations of N, N-dimethyl-1-methyldodecylamino oxide are relatively higher than the corresponding biostatic concentrations, but both still lie at pre-cellular concentrations of the amine oxide. The biocidal activity of Ν, Ν-dimatyl-1-methyldodecylamine oxide increases with the concentration of amine oxide with temperature and time of exposure.
Rozsiahlym výakumom sa zlatilo: Minimálna blocídna koncentrácia amínoxidu je 0,02 %; optimálně podmienky dezinfekčněj účinnosti N,N-dimetyl-l-metyldodecylamínoxidu aú v kyslej oblasti pH /3 až 5/ a vyšších teplotách prostredie /50 až 70 °C/. Za optimélpych podmienok při 0,02 % koncentrácii N,N-dimetyl-l-metyldodecylam£noxidu a době pdsobenia kratšej ako 5 minút, dosiahnutý stupeň inaktivácia mikroorganizmov je vyšší ako 99,999 % mrtvých buniek. Vysoký stupen dezinfekčněJ účinnosti možno dosiahnúť aj v alkalickom prostředí, reap. pri nižšej teplete vhodným zvýšením koncentrácie amínoxidu, resp. doby jeho pdsobenia.The major bloody gold concentration was: The minimum blocking amine oxide concentration was 0.02%; optimally the conditions of disinfecting activity of N, N-dimethyl-1-methyldodecylamine oxide in an acidic pH range (3 to 5) and higher ambient temperatures (50 to 70 ° C). Under optimum conditions at a concentration of 0.02% N, N-dimethyl-1-methyldodecylamine oxide and an exposure time of less than 5 minutes, the degree of inactivation of the microorganisms achieved is greater than 99.999% of dead cells. A high degree of disinfection efficiency can also be achieved in an alkaline environment, e.g. at lower heat by suitable increase of amine oxide concentration, resp. the time of its adaptation.
To isté platí i pri použití Ν,Ν-dimetyl-l-metyldodecylaijiínoxidu v znečiatenejšom prostředí, pričom prítomnoať organických látok v 1 % koncentrácii alebo iónov Ca2+, resp. Mg2+ The same applies to the use of Ν, Ν-dimethyl-1-methyldodecyl aniline oxide in a more polluted environment, whereby the presence of organic substances in 1% concentration or ions of Ca 2+ , resp. Mg 2+
205 043 v 50 mM koncentrácii nie je eSte na závadu. Zvýšením koncentrácie amínoxidu nad kritická micelárnu koncentráciu /0,05 95/ sa ustanovuje v roztokoch rovnováha medzi aktívnymf monomérmi a micelami /rezervoár aktívnych molekúl/, pričom súčasne sa dosahuje maximálně zníženie povrchového napfitia dezinfekčných roztokov, spojené so vzrastom ich eolubillzačných schopností.205 043 at 50 mM concentration is not a defect. By increasing the amine oxide concentration above the critical micellar concentration (0.05 95), the solutions establish an equilibrium between the active monomers and the micelles (reservoir of active molecules), while at the same time achieving a maximum reduction in surface tension of the disinfectant solutions associated with increasing their eolubility properties.
Táb. 1 Inhibícia rastu baktérií N,N-dimetyl-l-metyldodecylamínoxidom /300 /Ug/ po 72 hodinách rastu pri 37 °C.Tab. 1 Inhibition of N, N-dimethyl-1-methyldodecylamine oxide (300) Ug growth of bacteria after 72 hours of growth at 37 ° C.
Tab. 2 Inhibícia rastu vláknitých húb N,N-dimetyI-l-metyldodecylamínoxidom /300 yug/Tab. 2 Inhibition of growth of filamentous fungi with N, N-dimethyl-1-methyldodecylamine oxide (300 yug)
205 043205 043
Prakticky možno uskutočniť vynález podl’a nasledovných príkladov bez toho, že by sa len výlučné na ne obmedzoval.In practice, the invention may be practiced according to the following examples without being limited thereto.
Příklad 1 •7Example 1 • 7
Κ 1 ml suspenzie kvaainiek Saccharomyces cerevieiae /10' buniek/ v tekutorn kultivačnom médiu o pH 5, pri 35 °C sa přidal 0,1 ml 0,5 % N,N-dimetyl-l-metyldodecylamínoxid. Po dvoch minútach expozície při 35 °C počet přežitých buniek sa stanovil po zriedení výsevom na agarové platné. Log % přežitých buniek, charakterizujúci účinnosť inaktivačného postupu po expozícii bol menši ako -3,0.Ml 1 ml of suspension of Saccharomyces cerevieiae cells (10 'cells) in liquid culture medium, pH 5, at 35 ° C was added 0.1 ml of 0.5% N, N-dimethyl-1-methyldodecylamine oxide. After two minutes of exposure at 35 ° C, the number of survived cells was determined after dilution by sowing on agar plates. Log% survived cells characterizing the efficacy of the inactivation procedure after challenge was less than -3.0.
Příklad 2 ř Q 0Example 2 Q Q 0
K 0,1 ml tekutého živného másopeptonového média obsahujúceho zmes 5x10 aerobne vyrastených vegetatívnyoh buniek Bacillus cereus, Escherichia coll, Salmonella typhimurium, Staphylococcus aureus a Streptooocous faecalfa sa přidal 1 ml na 50 °C zahriateho 0,1 % N,N-dimetyl-l-metyldodeeylamínoxidu v 20 mM. Sorensenovom pufre o pH 7,0. Po platich minútach lnkubácie počet přežitých zárodkav sa stanovil po zriedení výsevom na agarové platné. Log % přežitých buniek, charakterizujúci účinnosť inaktivačného procesu po expozícii bol menší ako -4,7.To 0.1 ml of liquid nutritional masopepton medium containing a mixture of 5x10 aerobically grown vegetative cells of Bacillus cereus, Escherichia coll, Salmonella typhimurium, Staphylococcus aureus and Streptooocous faecalfa was added 1 ml at 50 ° C heated with 0.1% N, N-dimethyl-1. -methyldodeeylamine oxide in 20 mM. Sorensen buffer pH 7.0. After plating the minutes of incubation, the number of germ survivors was determined after dilution by sowing on agar plates. The log% survival of the cells, characterizing the efficiency of the inactivation process after exposure was less than -4.7.
Příklad 3-7Example 3-7
Κ 1 ml inaktivačnej zmesi obsahujúcej 0,02 % N,N-dimetyl-l-metyldodecylamínoxid, mM citrát-fosfátový pufor o pHT 4,0 pri tepláte 50 °C sa přidal 0,1 ml zmesnej mlkroflory /10® až 10® buniek/ vzniknutej pomnožením buniek odobratých sterovou metodou z rdznych prevádzok masokombinátu. Konoentráoia exponovaných a po inaktivácil přežitých buniek sa stanovila po zriedení výsevom na agarové platné· POvod mikroflóry, exponovaná konoentráoia mikroorganizmov a log % přežitých buniek pre jednotlivé případy inaktivačného postupu sú uvedené nižšle.Κ 1 ml of inactivation mixture containing 0.02% N, N-dimethyl-1-methyldodecylamine oxide, mM citrate-phosphate buffer pHT 4.0 at 50 ° C was added 0.1 ml of mixed microflora / 10® to 10® cells / resulting from the multiplication of cells harvested by the ster method from various meat processing plants. Concentration of exposed and after inactivated survival cells was determined after dilution by sowing on agar-validated microbial flora, exposed conjugation of microorganisms and log% survival cells for each case of inactivation procedure are shown below.
Příklad 8Example 8
Κ 1 ml inaktivačnej zmesi obsahujúcej 0,02 % N,N-dimetyl-l-metyldodecylamínoxid, mM sodnú sol’ kyseliny etyléndiamfnotetraoctovej pri pH 4,0 a teplote 50 °C sa přidal 0,1 ml zmesnej kultúry mikroorganizmov obsahujúcej 1,39x10? buniek, vzniknutej pomnožením a zmiešaním buniek odobratých sterovou metodou z kontájnerov, sušičky krve, podlahy a stien masokombinátu. Po dvoch minútach expozície log % přežitých buniek bol menší ako -3,14.Ml 1 ml of an inactivation mixture containing 0.02% N, N-dimethyl-1-methyldodecylamine oxide, mM sodium ethylenediamphnotetraacetic acid at pH 4.0 and 50 ° C was added 0.1 ml of a mixed microorganism culture containing 1.39x10? cells produced by the multiplication and mixing of the cells collected by the steric method from the containers, the blood dryer, the floor and the walls of the meat processing plant. After two minutes exposure to log% survived cells was less than -3.14.
205 643 t205 643 t
Příklad 9Example 9
Povrch kovověj přepravky v masokombináte; sa bez předběžného čistenla vystavil za stálého miešania štetcom, pri 50 °C na dobu 5 minút účinku 0,02 % N,N-dimetyl-l-metyldodecylamínoxidtt v 10 mM citrát-fosfátovom pufre o pH 4,0, aplikovaného v množstve 1 liter/m .The surface of the metal crate in the meat combine; was exposed to 0.02% N, N-dimethyl-1-methyldodecylamine oxide in 10 mM citrate-phosphate buffer, pH 4.0, applied at 1 liter at 50 ° C for 5 minutes without pre-cleaning with a brush. / m.
Po oplachu s rovnakým množstvom sterilnej vody sa mikroorganizmy z exponovaných pldch odobrali sterovou metodou a ich počet po vytrepaní do roztoku sa stanovil kultiváciou na pevných agarových mésdiách. Pri stanovení mikrobiálnej kontaminácie kontrolných pldch sa postupovalo obdobným spdsobom. Počet životaschopných záradkov v porovnaní s kontrolou poklesol 0,8x10^ krát.After rinsing with the same amount of sterile water, the microorganisms from the exposed plaques were collected by the sterile method and counted after shaking into solution by cultivation on solid agar media. Microbial contamination of the control pldch was determined in a similar manner. The number of viable pleats decreased 0.8x10-fold compared to control.
Příklad 10Example 10
Postupovalo ea ako v příklade 9. Inaktivovali sa mikroorganizmy na povrchu kovověj stolovej došky v masokombináte. Počet životaschopných záradkov po expozícii poklesol 1,5x10^ krát v porovnaní s kontrolou.The procedure was as in Example 9. Microorganisms were inactivated on the surface of a metal table thatched in a meat-aggregate. The number of viable plugs after exposure decreased 1.5x10-fold compared to control.
Přiklad 11Example 11
Inaktivačný postup ako v příklade 9. Inaktivovali sa mikroorganizmy na povrchu keramickej dlážky v prevádzkach mliekárenského priemyslu. Počet životaschopných zárodkov po expozícii poklesol 10^ krát v porovnaní s kontrolou.Inactivation procedure as in Example 9. Microorganisms on the surface of the ceramic floor were inactivated in dairy plants. The number of viable germs after exposure decreased 10-fold compared to control.
Přiklad 12Example 12
Inaktivačný postup ako v příklade 9. Inaktivovali ea mikroorganizmy na povrchu přepravky na mlieko. Počet životaschopných zárodkov po expozícii bol 7,4x10* krát nižší ako před expozíciou.Inactivation procedure as in Example 9. They inactivated ea microorganisms on the surface of the milk container. The number of viable germs after exposure was 7.4x10 * times lower than before exposure.
N,N-dimetyl-l-metyldodecylamínoKÍd má výraznú biocídnu aktivitu, povrchovo aktivně a solubilizačná vlastnosti, nízku toxicitu, kompatibilitu s tenzidmi a dobrú rozpustnost vo vodě.N, N-dimethyl-1-methyldodecylaminoKid has significant biocidal activity, surfactant and solubilizing properties, low toxicity, surfactant compatibility and good water solubility.
N,N-dimetyl-l-metyldodecylamínoxfd /0,02 - 2,0 %/ je ako biologicky účinná látka použitelný k přípravě dezinfekčných prostriedkov, ako aj konzervačných a šolubilizačných činidiel /obzvlášť v kozmetike/. Dezinfekčně prostriedky na báze amínoxidov sú aplikovatelné v potravinárakom priemysle, v zdravotnictvo a všade tam, kde sa udržuje hygiena. Dezinfekciu možno uskutočnovať v uzavretom alebo otvoronom systéme, a to bud až po dSkladnom čistění, alebo; vykonávat čistenie a dezinfekciu v kyslom prostředí v jedinom stupni, avšak pri relativné vyšších koncentráciách amínoxidu a vhodných ingpedientov sanitačných zmesi.N, N-dimethyl-1-methyldodecylamino oxide (0.02-2.0%) is useful as a biologically active substance for the preparation of disinfectants, as well as preservatives and solubilizers (especially in cosmetics). Amine oxide-based disinfectants are applicable in the food industry, healthcare and wherever hygiene is maintained. Disinfection can be carried out in a closed or open system, either after thorough cleaning, or; carry out the cleaning and disinfection in an acidic environment in a single stage, but at relatively higher concentrations of amine oxide and suitable sanitary mix ingents.
V potravinářstve možno dezinfekčně prostriedky na báze N,N-dimetyl-l-metyldodecylamínoxidu použit najmě v mfisovom, mliekárenskom, hydinárskom, konzervárenskom a fermentačnom priemysle, rovnako ako v pivovaroch alebo vinéfrskych závodoch. V koncentráciách 0,02 až 0,2 % pri dodržiavsní podmienok optimálnej aktivity možno N,N-dimetyl-l-metyIdodecylamínoxid použiť k dezinfekci! výrobných priestorov, zariadení a nástrojov v závodoch potravinářskéhoIn the food industry, N, N-dimethyl-1-methyldodecylamine oxide disinfectants can be used, in particular, in the dairy, dairy, poultry, canning and fermentation industries, as well as in breweries or wineries. N, N-dimethyl-1-methyl dodecyl amine oxide can be used for disinfection at concentrations of 0.02 to 0.2% under optimal activity conditions. production facilities, equipment and tools in food factories
205 843 priemyslu. So úvahy prichádza povrchová dezinfekcia rúk, tankov, kaniev, potrubí, púmp, plniδlek, ohrievačov, chladičov, separétorov, homogenizátorov, stlán, podláh a stropov, výrobných miestnosti, dletribučných vozidlel, prepraviek, obalov, použitelný je ej pri atabilizáoii surovin, cirkulujúoich vdd e pod.205 843 industry. Surface disinfection of hands, tanks, cannulae, pipes, pumps, fillers, heaters, coolers, separators, homogenizers, tables, floors and ceilings, production rooms, detrribution vehicles, crates, packaging, for use in the circulation of raw materials, e pod.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS890578A CS205643B1 (en) | 1978-12-27 | 1978-12-27 | Method of inactivation of the microorgasms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS890578A CS205643B1 (en) | 1978-12-27 | 1978-12-27 | Method of inactivation of the microorgasms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CS205643B1 true CS205643B1 (en) | 1981-05-29 |
Family
ID=5440249
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS890578A CS205643B1 (en) | 1978-12-27 | 1978-12-27 | Method of inactivation of the microorgasms |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS205643B1 (en) |
-
1978
- 1978-12-27 CS CS890578A patent/CS205643B1/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4715980A (en) | Antimicrobial sanitizing composition containing n-alkyl and n-alkenyl succinic acid and methods for use | |
| Fredell | Biological properties and applications of cationic surfactants | |
| EP3136861B1 (en) | Composition containing peroxide and an antimicrobial agent and process of killing spores | |
| US6793846B2 (en) | Microbicide compositions | |
| US5593681A (en) | Glycine anhydride dimethylol as a biocide and preservative | |
| US9351488B2 (en) | Peracetic acid composition | |
| Lopes | Evaluation of dairy and food plant sanitizers against Salmonella typhimurium and Listeria monocytogenes | |
| US20030235623A1 (en) | Aqueous disinfecting compositions with rapid bactericidal effect | |
| US6054139A (en) | Cleaning and/or disinfecting composition | |
| CN103562368A (en) | Broad spectrum disinfectant | |
| EP1382666A1 (en) | Aqueous disinfecting compositions with rapid bactericidal effect | |
| KR100196460B1 (en) | Antibacterial agent containing quaternary ammonium salt | |
| WO2021005897A1 (en) | Biofilm treatment agent and biofilm treatment method | |
| US6610248B1 (en) | Disinfectants | |
| CN102302033A (en) | Preparation and application of compounded disinfection atomization agent | |
| JP3607652B2 (en) | Disinfectant composition | |
| RU2194072C2 (en) | Disinfectant-detergent | |
| EP0799570A1 (en) | Preparation and uses of microbicidal formulations | |
| CS205643B1 (en) | Method of inactivation of the microorgasms | |
| US4753749A (en) | Microbiocidal cleaning agent and preparation thereof | |
| EP1375634A1 (en) | Aqueous disinfecting compositions with rapid bactericidal effect | |
| EP1393629A1 (en) | Aqueous disinfecting compositions based on quaternary ammonium monomers | |
| NZ225589A (en) | Control of microorganisms in aqueous systems using 1-hydroxymethylpyrazoles | |
| JP3607601B2 (en) | Disinfectant composition | |
| CN106118905A (en) | A kind of high-effective disinfecting liquid detergent |