CS205192B1 - Preparation method of standard solution of thyroxine for radioimmunoanalitical examination unit - Google Patents

Description

(54) spdeob for the preparation of standard thyroxine solutions for radioimmunoassay examination

The invention relates to an epaeob for the preparation of standard thyroxine solutions for the radiolmunoanalytically investigated treatment by the concentration of nenograms in milliliters. and a precisely determined relative deviation from the declared value).

An important component of the thylaroxyanalytical examination preparations on etanovenle thyroxine in the urine is the standard thyroxine solutions and pre-known concentrations. The difficulty of their preparation is given by the fact that the light conontrusion ea moves in the oe by the concentration of thyroxine in the oor, which is in the range from 25 to 300 µmol.m** ³ · This eliminates the possibility of using conventional methods of preparation and control.

The dopoolel processes used to prepare standard thyroxine solutions were based on the smallest possible overload of thyroxine. Depending on its size aa, by dissolving it with a suitable flush, it prepared a solution of thyroxine with a predetermined concentration. From this solution 1 ml was tapped and made up to a volume of 250 ml. By this procedure, a standard solution was obtained with the highest concentration required. The other granulated and lured standard solutions with a low concentration of thyroxine. This procedure was laborious, wavering for the preparation of a small number of standards and, in addition, did not allow ziatonia and elimination of standards deprived of rough random bending. The sloop irreducible -for the preparation of the standards has influenced decisive epdeobom accuracy and self-analysis of the immunoanalysis, thereby significantly destroying the kllnloko-dlagnoetlokč usability of the radioluneanalysis treatment.

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These disadvantages have been eliminated for the preparation of thyroxine solutions for radlolaunoanalytocyclic scavenger treatment, the preparation of which is based on the preparation of 10 to 40, preferably 20, of thyroxine in the range of from 1 to 20 ag per 1000 al of the basic spacing, weight and dissolution in the canoe. ammonia spill in paaara 6 to 100% vol. parts per 1000 vol. parts of the solution, make up and de-fill with water, check and take the thyroxine conjugate epoctrophoteatres in these stock solutions and discard these solutions, which show a deviation of more than 4% from the throat and the weight of the y + bx the most recent quadrices and where x i sxtlnkela, y i am rich thyroxine weighs, oab eu constants, patea and a stock solutions will blend to a prone cononontraion in the range from 0.6 to 60.0 ng.al '\ , preferably a solution satisfying the equilibrium linearized equilibrium equation and having a deviation therefrom of up to 10%.

The dental abilities of the standards (theoretically 1 000 000 po 1 al) by a deviation of five times aonea from the declared value in the pre-treatment of the preparation, and by the work and the height of the reproduction, the operation and the operation of the invention preparation and makes it possible to date standard solutions and accidentally bend it as I declared.

The above procedure is also suitable for the preparation of standard radiolaunoanalytical examinations.

Priklad prevedenia.

It made about 20 weighings (proono) of thyroxine, rewnoorally distributed in the intarvals of 1.00 to 14.00 g - weighing no. lt 1,21 ag, weight 6. 20t 12,71 ag. The weights and quantitative predominations were up to 1000 and alllkoňovanýeh odaornýoh mines. The batches were dissolved and added with 10 [mu] l of an aqueous conjugated aaonlakate solution. After a few minutes, the odorless flasks were made up to 1000 [mu] l with additional water.

It made apoctrophotoaotropic atanovano conoontraelo thyroxine flux, which in UV-eblaatl provided about every solution at 226 ° C. The deceptive formwork oxtlnkcll was made to roll around on haotnaatl landings. As coarse creases aa cured solutions from Salila's preparation process, danoa attaches solutions no. 8 and 6. 15, which is a variation coofl-; olat from folded folded jerking stabbed aatode najaoailoh four-legged yeah eye eye eyebrows relative bends weighed, aaranla oxtlnkolo and relative bends of the beaker, which amounts to weighings over 2.00g and 3.7% and below 2.00g and from 3.7 to 6 , 1%.

Of the solutions, the solutions were flushed with 1 to 1000 l aillkonovanoj odaernoj containers and added to a 1000 to 0.08 M barbital pressure solution (pH and 8.6) and both 0.2% bovine line albual. Tyne aa has obtained a set of standards of 1000 ka (1 al) and concentrate ranging from 1.00 to 14.00 ng.al ** ^ ·

From each set of samples and the same keneentral, and took 10 samples of standards.

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Using a radioimmunoassay treatment, 10 calibration dependences were constructed using selected samples. These dependencies were transformed into legit-log fertile specimens, which statistically significantly deviated from the crack dependence in these sub-axes. As a result of this inspection phase, Siestich label no. 5,4,9,12,14 & 17 ·

Standard solutions with a relative deviation of - 5% from the declared value (relative deviations depend on the weights used, the spectrophotometer and the rubbing glass used) are scared.

Claims (1)

OBJECT OF THE INVENTION Process for the preparation of standard thyroxine solutions for a reduloinunoanalytical screening treatment, characterized in that ea prepares 10 to 40, and preferably 20, the weight of thyroxine in the range of from 1 to 20 ag per 1000 al of the basic solution, the batches dissolved in concentrated aqueous ammonia solution. in the range of 5 to 100 vol., parts to 1000 vol. of solution, make up with distilled water, check the thyroxine concentration in these buffer solutions in the electrolytic tubes and discard the further solutions which they exhibit from further processing; deviation above 4% from the given y y * bx relationship, obtained from the well-worthy t axtinkcle depending on the thyroxine weighing method and where x is the extinction, y is the thyroxine weighting, and, b eu constants, then the stock solutions are fed to The concentration of thyroxine aa is checked by radio-immunoassay and of the aa and solutions, and preferably of the solutions, satisfying the linearized calibrated dependence equation and having a deviation from it of up to 10%.