CO6710901A2 - Método de vitrificación de superficie con nitrógeno líquido - Google Patents

Método de vitrificación de superficie con nitrógeno líquido

Info

Publication number
CO6710901A2
CO6710901A2 CO13051612A CO13051612A CO6710901A2 CO 6710901 A2 CO6710901 A2 CO 6710901A2 CO 13051612 A CO13051612 A CO 13051612A CO 13051612 A CO13051612 A CO 13051612A CO 6710901 A2 CO6710901 A2 CO 6710901A2
Authority
CO
Colombia
Prior art keywords
droplet
vitrified
medium
vitrification
liquid nitrogen
Prior art date
Application number
CO13051612A
Other languages
English (en)
Inventor
Fuliang Du
Jie Xu
Juan Moreno
Original Assignee
Inguran Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inguran Llc filed Critical Inguran Llc
Publication of CO6710901A2 publication Critical patent/CO6710901A2/es

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0236Mechanical aspects
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0205Chemical aspects
    • A01N1/021Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0205Chemical aspects
    • A01N1/021Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0278Physical preservation processes
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0278Physical preservation processes
    • A01N1/0284Temperature processes, i.e. using a designated change in temperature over time
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0604Whole embryos; Culture medium therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0608Germ cells
    • C12N5/0609Oocytes, oogonia

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Mechanical Engineering (AREA)
  • Physiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Un método de vitrificación de superficie con nitrógeno líquido que requiere un embrión lavado en un medio de enjuague, luego incubado en un medio base e incubado en un medio de mantenimiento antes de ser lavado en un medio de vitrificación y producido dentro de una gota de vitrificación. La gota de vitrificación puede ser producida de un instrumento con un canal y dejado caer directamente dentro de nitrógeno en fase liquida produciendo una gota vitrificada. La gota vitrificada puede ser almacenada en recipientes criogénicos, y calentada para revitalización de la función biológica de masas de células biológicas vitrificadas o tejidos, como ovocitos y/o embriones.
CO13051612A 2010-08-20 2013-03-14 Método de vitrificación de superficie con nitrógeno líquido CO6710901A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37571610P 2010-08-20 2010-08-20

Publications (1)

Publication Number Publication Date
CO6710901A2 true CO6710901A2 (es) 2013-07-15

Family

ID=44678026

Family Applications (1)

Application Number Title Priority Date Filing Date
CO13051612A CO6710901A2 (es) 2010-08-20 2013-03-14 Método de vitrificación de superficie con nitrógeno líquido

Country Status (7)

Country Link
US (1) US8633023B2 (es)
EP (1) EP2605644B1 (es)
AU (1) AU2011291993B2 (es)
BR (1) BR112013003929A2 (es)
CA (1) CA2808101C (es)
CO (1) CO6710901A2 (es)
WO (1) WO2012024408A2 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0120645D0 (en) 2001-08-24 2001-10-17 Smiths Group Plc Medico-surgical devices
GB0307350D0 (en) 2003-03-29 2003-05-07 Smiths Group Plc Catheters
DE102012013267A1 (de) 2012-07-04 2014-01-09 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Substrateinrichtung, Konservierungsgerät und Verfahren zur Kryokonservierung einer biologischen Probe
US9723831B2 (en) 2014-07-30 2017-08-08 The Governing Council Of The University Of Toronto System and methods for automated vitrification of biological materials
US10531657B2 (en) 2015-12-07 2020-01-14 Coopersurgical, Inc. Low temperature specimen carriers and related methods
AU2018374778B2 (en) 2017-11-28 2023-03-02 Coopersurgical, Inc. Specimen containers and related methods
NZ770478A (en) * 2018-05-31 2023-05-26 Vitalant Methods and systems for cryopreservation and resuspension of body fluids
US11116206B2 (en) 2018-10-01 2021-09-14 Cook Medical Technologies Llc Cryocontainer
CA3133252A1 (en) * 2019-03-13 2020-09-17 Membrane Protective Technologies, Inc. Methods and systems for protective supplementation during temperature depression
WO2021040870A1 (en) * 2019-08-30 2021-03-04 Coopersurgical, Inc. Reproductive specimen processing systems and methods

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6982172B2 (en) 2000-01-04 2006-01-03 University Of Connecticut Oocyte vitrification technique
EP1131998A1 (en) 2000-03-08 2001-09-12 Director-General Of National Institute Of Animal Industry, Ministry Of Agriculture, Forestry And Fisheries Cell-cryopreservation method
US20070037271A1 (en) * 2005-08-15 2007-02-15 Jang-Chi Huang Vitrification apparatus for microdrop vitrification of cells and a method of microdrop vitrification of cells using the apparatus
WO2007123720A2 (en) * 2006-03-30 2007-11-01 Cornell Research Foundation, Inc. System and method for increased cooling rates in rapid cooling of small biological samples
US20100216230A1 (en) * 2006-09-28 2010-08-26 Cornell Research Foundation, Inc. Systems for Increased Cooling and Thawing Rates of Protein Solutions and Cells for Optimized Cryopreservation and Recovery
EP2156735A1 (en) 2008-08-13 2010-02-24 Dr. H. Zech GmbH Method and instrument for vitrification and storing of biological specimen

Also Published As

Publication number Publication date
US8633023B2 (en) 2014-01-21
EP2605644B1 (en) 2014-06-25
WO2012024408A2 (en) 2012-02-23
AU2011291993A1 (en) 2013-02-21
EP2605644A2 (en) 2013-06-26
AU2011291993B2 (en) 2013-12-19
WO2012024408A3 (en) 2012-10-04
US20130157362A1 (en) 2013-06-20
CA2808101A1 (en) 2012-02-23
BR112013003929A2 (pt) 2017-11-07
CA2808101C (en) 2018-01-23

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