CO4370115A1 - METHOD AND APPARATUS FOR POSITIVE OR NEGATIVE SELECTION OF A POPULATION OR SUB-POPULATION OF A SAMPLE USING PARTICLES AND GRAVITY SEDIMENTATION - Google Patents

METHOD AND APPARATUS FOR POSITIVE OR NEGATIVE SELECTION OF A POPULATION OR SUB-POPULATION OF A SAMPLE USING PARTICLES AND GRAVITY SEDIMENTATION

Info

Publication number
CO4370115A1
CO4370115A1 CO95013790A CO95013790A CO4370115A1 CO 4370115 A1 CO4370115 A1 CO 4370115A1 CO 95013790 A CO95013790 A CO 95013790A CO 95013790 A CO95013790 A CO 95013790A CO 4370115 A1 CO4370115 A1 CO 4370115A1
Authority
CO
Colombia
Prior art keywords
population
sub
particles
sample
positive
Prior art date
Application number
CO95013790A
Other languages
Spanish (es)
Inventor
Wallace H Coulter
Robert K Zwerner
Robert J Schmittling
Russell Thomas R
Original Assignee
Coulter Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coulter Corp filed Critical Coulter Corp
Publication of CO4370115A1 publication Critical patent/CO4370115A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/824—Immunological separation techniques

Abstract

1. UN METODO PARA RETIRAR UNA POBLACION O SUBPO- BLACION SELECCIONADA DE UNA MUESTRA DE FLUIDO, QUE COMPRENDE: PROPORCIONAR UNA PLURALIDAD DE PARTI- CULAS QUE TIENEN UNA DENSIDAD SUFICIENTE PARA PRO- PORCIONAR ASENTAMIENTO DIFERENCIAL POR GRAVEDAD, DICHAS PARTICULAS QUE TIENEN UNIDAS ALLI UN REAC- TIVO ESPECIFICO A POR LO MENOS UNA POBLACION O SUBPOBLACION SELECCIONADA; MEZCLAR UNA PORCION DE LA DICHA MUESTRA CON LAS DICHAS PARTICULAS PARA ENLAZAR LAS DICHAS PARTICULAS A LA DICHA POBLACION O SUBPOBLACION PRE-SELECCIONADA SIN ANADIR SUSTAN- CIALMENTE EN FORMA CRITICA LA DICHA POBLACION O SUBPOBLACION SELECCIONADA; ASENTAR POR GRAVEDAD DIFERENCIAL LAS DICHAS PARTICULAS CON LA DICHA PO- BLACION UNIDA O SUBPOBLACION EN LA DICHA PORCION DE MUESTRA; Y SEPARAR POR LO MENOS UNA PORCION DEL SOBRENADANTE RESULTANTE DE LA DICHA PORCION DE MUESTRA INCLUYENDO LA DICHA POBLACION O SUBPOBLA- CION NO SELECCIONADA DE LAS DICHAS PARTICULAS Y LA DICHA POBLACION O SUBPOBLACION ENLAZADA.1. A METHOD FOR REMOVING A SELECTED POPULATION OR SUB-POPULATION FROM A FLUID SAMPLE, WHICH INCLUDES: PROVIDING A PLURALITY OF PARTICLES THAT HAVE A DENSITY SUFFICIENT TO PROVIDE DIFFERENTIAL SETTLEMENT BY GRAVITY, THESE PARTICLES HAVE ONE UNIT SPECIFIC REAGENT TO AT LEAST ONE SELECTED POPULATION OR SUB-POPULATION; MIXING A PORTION OF THE SAID SAMPLE WITH THE SAID PARTICLES TO LINK THE SAID PARTICLES TO THE SUCH PRE-SELECTED POPULATION OR SUB-POPULATION WITHOUT SUBMITTING SUBSTANTIALLY CRITICALLY THE SAID POPULATION OR SUB-POPULATION SELECTED; SETTING THOSE PARTICULARS WITH DIFFERENTIAL GRAVITY WITH THE SAID UNITED POPULATION OR SUB-POPULATION IN THE SUCH SAMPLE PORTION; AND SEPARATE AT LEAST ONE PORTION OF THE OVERSIZE RESULTING FROM THE SAID PORTION OF SAMPLE, INCLUDING THE SUCH UNSELECTED POPULATION OR SUB-POPULATION OF THE SUCH PARTICLES AND THE SUCH LINKED POPULATION OR SUB-POPULATION.

CO95013790A 1994-04-15 1995-04-05 METHOD AND APPARATUS FOR POSITIVE OR NEGATIVE SELECTION OF A POPULATION OR SUB-POPULATION OF A SAMPLE USING PARTICLES AND GRAVITY SEDIMENTATION CO4370115A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/228,791 US5576185A (en) 1994-04-15 1994-04-15 Method of positive or negative selection of a population or subpopulation of a sample utilizing particles and gravity sedimentation

Publications (1)

Publication Number Publication Date
CO4370115A1 true CO4370115A1 (en) 1996-10-07

Family

ID=22858583

Family Applications (1)

Application Number Title Priority Date Filing Date
CO95013790A CO4370115A1 (en) 1994-04-15 1995-04-05 METHOD AND APPARATUS FOR POSITIVE OR NEGATIVE SELECTION OF A POPULATION OR SUB-POPULATION OF A SAMPLE USING PARTICLES AND GRAVITY SEDIMENTATION

Country Status (15)

Country Link
US (2) US5576185A (en)
EP (1) EP0756709B1 (en)
JP (1) JPH10502011A (en)
CN (1) CN1155828C (en)
AU (1) AU700437B2 (en)
BR (1) BR9507373A (en)
CA (1) CA2187543A1 (en)
CO (1) CO4370115A1 (en)
DE (1) DE69529462T2 (en)
IL (1) IL113265A (en)
NO (1) NO964362L (en)
RU (1) RU2141664C1 (en)
TW (1) TW313627B (en)
WO (1) WO1995028643A1 (en)
ZA (1) ZA952723B (en)

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Also Published As

Publication number Publication date
NO964362D0 (en) 1996-10-14
NO964362L (en) 1996-10-14
US6900029B1 (en) 2005-05-31
US5576185A (en) 1996-11-19
DE69529462D1 (en) 2003-02-27
ZA952723B (en) 1996-04-22
WO1995028643A1 (en) 1995-10-26
EP0756709B1 (en) 2003-01-22
AU2479995A (en) 1995-11-10
CN1150841A (en) 1997-05-28
EP0756709A4 (en) 1998-12-02
IL113265A0 (en) 1995-07-31
IL113265A (en) 1999-05-09
BR9507373A (en) 1997-09-30
JPH10502011A (en) 1998-02-24
EP0756709A1 (en) 1997-02-05
TW313627B (en) 1997-08-21
DE69529462T2 (en) 2003-10-02
RU2141664C1 (en) 1999-11-20
AU700437B2 (en) 1999-01-07
CN1155828C (en) 2004-06-30
CA2187543A1 (en) 1995-10-26

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