CO2024000351A2 - Secuenciación de novo del terminal n de proteína mediante dimetilación selectiva de la posición - Google Patents
Secuenciación de novo del terminal n de proteína mediante dimetilación selectiva de la posiciónInfo
- Publication number
- CO2024000351A2 CO2024000351A2 CONC2024/0000351A CO2024000351A CO2024000351A2 CO 2024000351 A2 CO2024000351 A2 CO 2024000351A2 CO 2024000351 A CO2024000351 A CO 2024000351A CO 2024000351 A2 CO2024000351 A2 CO 2024000351A2
- Authority
- CO
- Colombia
- Prior art keywords
- dimethylation
- selective
- protein
- terminus
- novo sequencing
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 238000012163 sequencing technique Methods 0.000 title 1
- 102400000108 N-terminal peptide Human genes 0.000 abstract 2
- 101800000597 N-terminal peptide Proteins 0.000 abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 150000002500 ions Chemical class 0.000 abstract 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6818—Sequencing of polypeptides
- G01N33/6824—Sequencing of polypeptides involving N-terminal degradation, e.g. Edman degradation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Peptides Or Proteins (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención se refiere en general a métodos para determinar la secuencia de aminoácidos de una proteína. En particular, la presente invención se refiere al uso de dimetilación selectiva de la posición y cromatografía de líquidos-espectrometría de masas para mejorar la señal de péptidos del terminal N y cambiar la señal de los péptidos del terminal N y los iones b correspondientes, para así facilitar una determinación de la secuencia de péptidos del terminal N.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163221454P | 2021-07-13 | 2021-07-13 | |
| PCT/US2022/036876 WO2023287828A1 (en) | 2021-07-13 | 2022-07-12 | Protein n-terminal de novo sequencing by position-selective dimethylation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO2024000351A2 true CO2024000351A2 (es) | 2024-02-05 |
Family
ID=82846366
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CONC2024/0000351A CO2024000351A2 (es) | 2021-07-13 | 2024-01-16 | Secuenciación de novo del terminal n de proteína mediante dimetilación selectiva de la posición |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20230032607A1 (es) |
| EP (1) | EP4370928A1 (es) |
| JP (1) | JP2024526736A (es) |
| KR (1) | KR20240032972A (es) |
| CN (1) | CN117859065A (es) |
| AU (1) | AU2022309876A1 (es) |
| CA (1) | CA3225730A1 (es) |
| CO (1) | CO2024000351A2 (es) |
| IL (1) | IL309774A (es) |
| WO (1) | WO2023287828A1 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201715684D0 (en) * | 2017-09-28 | 2017-11-15 | Univ Gent | Means and methods for single molecule peptide sequencing |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US1522343A (en) | 1923-05-02 | 1925-01-06 | Thom Clarence | Magnetic separator |
| WO2003031928A2 (en) * | 2001-07-20 | 2003-04-17 | Diversa Corporation | Cellular engineering, protein expression profiling, differential labeling of peptides, and novel reagents therefor |
| GB0130228D0 (en) | 2001-12-18 | 2002-02-06 | Hansa Medica Ab | Protein |
| AU2020368409A1 (en) * | 2019-10-15 | 2022-05-19 | Regeneron Pharmaceuticals, Inc. | Methods for characterizing host-cell proteins |
-
2022
- 2022-07-12 EP EP22751906.3A patent/EP4370928A1/en active Pending
- 2022-07-12 US US17/863,349 patent/US20230032607A1/en active Pending
- 2022-07-12 CA CA3225730A patent/CA3225730A1/en active Pending
- 2022-07-12 WO PCT/US2022/036876 patent/WO2023287828A1/en active Application Filing
- 2022-07-12 KR KR1020247004479A patent/KR20240032972A/ko unknown
- 2022-07-12 JP JP2024501684A patent/JP2024526736A/ja active Pending
- 2022-07-12 AU AU2022309876A patent/AU2022309876A1/en active Pending
- 2022-07-12 IL IL309774A patent/IL309774A/en unknown
- 2022-07-12 CN CN202280049380.0A patent/CN117859065A/zh active Pending
-
2024
- 2024-01-16 CO CONC2024/0000351A patent/CO2024000351A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN117859065A (zh) | 2024-04-09 |
| IL309774A (en) | 2024-02-01 |
| CA3225730A1 (en) | 2023-01-19 |
| EP4370928A1 (en) | 2024-05-22 |
| KR20240032972A (ko) | 2024-03-12 |
| US20230032607A1 (en) | 2023-02-02 |
| JP2024526736A (ja) | 2024-07-19 |
| AU2022309876A1 (en) | 2024-01-18 |
| WO2023287828A1 (en) | 2023-01-19 |
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