CN87102613A - Microculture device for plant - Google Patents

Abstract

A kind of device of minimum plant breeding comprises sterilized space and sterilization cultivation vessel.This device is applicable to and carries out the biotechnology operation in specialized industry and research laboratory factory.

Description

The present invention relates to comprise the Microculture device for plant of a sterilized space and disinfectant cultivation vessel.

Known these methods are listed biological technical field usually in, are the forward positions that is in biological study work and the research of relevant scientific development thereof.Biotechnological means is particularly important in the tissue of plant and the training of cell unit are grown, and this method also can be applicable to study botanical system.

At the general characteristic of the microculture of tissue and cell unit breeding, in the cultivation vessel that under aseptic condition, carry out, sterilizing, in glassware, carry out, to reach the aseptic degree of cleaning of biology.

The importance of the method for the microculture in glassware was generally acknowledged by the different department in the agricultural already, and they have been used in many kinds of cultures and global experience and have promoted their development.

The scientific research of the microculture aspect biological is showing the development of height recently, but this technology and technical background still keep the state that falls behind simultaneously.Fine basis is arranged on the biological knowledge, yet the technological method that adopts but needs exploitation.Microculture method is in actual applications operated a large amount of plant varieties and is suitable difficulty, need careful because adopting in advance technology to compare with present phytological technological method to obtaining data, need with a kind of be very careful and effective means really.Plant and microculture are being produced and are being kept a large amount of (until 10 ⁶) be crucial in FFI heredity-homologous plant.

The plant microculture comprises the steps:

-preparation developing medium

-sterilization

-under gnotobasis, transplant-this is that manual operations-sealing has plant unit

Vessel.

-cultivate

The above-mentioned step of mentioning usually need identical equipment and aspect microorganism a series of instrument and equipment, for example: dress is cultivated component and is solidified the glassware of component developing medium, and described developing medium is warm.Breathe but can not allow spore enter vessel owing to will guarantee nutrient solution, these vessel adopt cotton one wool stopper and through foil sealing commonly used.Transplanting ties up in the inoculation tank and finishes, and the product of operating under aseptic condition is transferred to the local of cultivation thus and cultivated.

Can see having in the operation and severally carry out under aseptic condition, and wherein some operation such as: the preparation of developing medium, join in the vessel and the finishing of sealing vessel, be in contrast, do not carry out under aseptic condition.Transplanting needs to sterilize before and later cultivation also can be carried out under non-sterile condition.

This sterilization and non-disinfectant means can be used in laboratory study and exploitation, but all will run into other requirement in mass production.So will change many strength for the validity that improves microculture.

Walked an important step in this respect by preparation developing medium in special factory.Below one to further develop be to prepare the solidification component of developing medium and do not need the component of warm developing medium again with the practice of other component mixings-this scheme of medium before application with the step of separating, important is the heat solid component.

Glassware is replaced by the container of lightweight transparent plastics system in some institutes, is replaced by foam plug, plastic coating unit, parafilm (Para film) or PVC paper tinsel with cotton-woolen sealing.

Step above-mentioned itself be very important, but every operation needs very carefully to finish by hand and must one have well-educated Knowledge Base.This means the very high requirement of the member that is engaged in these work and limited mass-produced application possibility in factory.

The object of the present invention is to provide a kind of method, can satisfy the purification requirement with the similar strong biology of known arrangement whereby, to improve the efficient of microculture.

The present invention is based on following understanding, when traditional working order of microculture will obtain one when being used for high-efficiency appliance that the minimum plant training grows and being changed to following manner, promptly prepare after the developing medium, operating under the aseptic condition under connecing carried out, this medium of sterilizing, required component pack into vessel, transplant this plant and seal vessel.This cultivation does not need aseptic condition.

So the microculture device of plant is a purpose of the present invention, it comprises a sterilized space, the cultivation vessel of sterilizing, hold charging mechanism to advance the storage unit of cultivating vessel, in storage unit, guarantee for example to cultivate the vessel disinfectant: ultraviolet lamp device, be arranged on below the charging mechanism or the transport unit around it, the storage unit of the cultivation component that dress was sterilized, the storage facility of the curing component that dress was sterilized, at least one intake machine connects mutually with stowage arrangement and storage unit, a mixing head connects mutually with an intake machine that comprises an outlet of opening at least, thermostat is used to the temperature that guarantees that storage unit is required, and this storage unit is preferably in first assembling that is included in a supporter above the transport unit and goes up the sealing paillon foil, and the sealed cylindrical puopulsion unit of the cultivation vessel that are used to sterilize is used for advancing the sealing paillon foil, heater strip is placed near the cylinder puopulsion unit and a thermal treatment unit and/or second group of storage unit are used to adorn sealing plug, dosage unit is used to advance the sealing plug, be used to guarantee sealing plug disinfectant device, and the tightness system of fixing seal plug on the cultivation vessel that are used for if desired.

The device that requires according to the present invention preferably adopts the transport unit that has convex surfaces, a tube unit to be used to link mixing head and at least one charging head and to be equiped with slide plate below feeding mechanism.

In preferred embodiments of the present invention, multidirectional liner and transport unit are set in the transport unit exit connect with liner further with preferable convex surfaces.

The present invention will give more detailed description in conjunction with indicated some preferred embodiments with reference to the accompanying drawings, and embodiment is as an illustration purpose only.In the accompanying drawing that is disclosed:

The assembling side elevation of Fig. 1, expression apparatus of the present invention, and

The assembling top view of Fig. 2, presentation graphs 1 device.

Device according to the object of the invention is a laboratory equipment that is used to guarantee a sterilized space with airtight outside surface.Outside surface for example can adopt: veneer case, thin-sheet barrel road or employing thin plate chamber are built up.

The cultivation vessel that are used for the sterilization of this device purpose are made with light transparent or plastic opaque, and for example: polyethylene, polypropylene, polycarbonate etc. are made.They are preferably the vessel of other shape that rectangular box can side by side place.Cultivating vessel is arranged at and is in side by side in the storage unit [1] or on an axis.

Charging mechanism [2] connects mutually with the outlet of (Fig. 1) storage unit [1], and device [3] guarantees that the sterilizing unit [3] of cultivating vessel can adopt a kind of ultraviolet lamp herein.

The outlet of slide plate [4] binding charging mechanism [2] and transport unit [5] are used for transmitting the cultivation vessel and enter next operation steps.If desired, multidirectional liner [10] can be arranged on the end of transport unit [5], is used for selecting to cultivate vessel according to different working direction.Can predict in this case and further need transport unit [11].

Before being transmitted before transport unit [5] go up to be cultivated vessel, at least one charging [71] on corresponding storage unit [61] and the storage unit [62] of mixing head [8], [72] are pack into cultivation component of sterilizing and the curing component of sterilizing (Fig. 2).Storage unit [61] and storage unit [62] are arranged on and guarantee their required temperature in corresponding thermostat [91], [92].

The cultivation vessel that are full of developing medium are pushed to their device of sterilization sealing.This realizes by rights: for example adopt PVC paillon foil sterilize to be cut into the sheet of desired length with heater strip [14], leave cylinder puopulsion unit [13] then or adopt the device [17] that is arranged on storage unit [15] be used for guaranteeing sealing with stopper sterilization and employing propelling sealing plug to seal the vessel feeder.If desired, on the cultivation vessel, press sealing plug by tightness system [18].

In the aseptic binding of cultivating between vessel and the PVC paillon foil is to adopt heating unit [19] to guarantee with blowing warm air.Sealing PVC paillon foil device is on supporting element [12].Certainly, if desired, paillon foil can adopt other suitable material to make.

According to the device of the object of the invention, can use according to following method:

Should using appts [3] before starting working to guarantee sterilization, if necessary, sterilized space that should finishing device.Device [3] and [17] be made up of ultraviolet lamp are helpful in this operation.A subsequent step be with cultivate vessel be placed into storage unit [1] and with the storage unit [61] that the cultivation component of sterilize is housed add stub bar [71] binding.The curing component that will be contained in the sterilization of storage unit [62] is heated to liquid state, and in sterilized space, add then stub bar [72] can and storage unit [62] link.Adding stub bar [71], [72] should be with such as connecting by hose element and mixing head [8].Storage unit is made the form that can be placed in thermostat [91], [92] in order to guarantee their required temperature.Supportive device [12] if desired, is equipped with PVC paillon foil volume, and a unsettled end of paillon foil is fixed on the cylinder puopulsion unit [13], near the surrounding environment of heater strip [14].If use, storage unit [15] should be full of the sealing plug that the suitable plastic of arrangement is side by side made.Be connected with a control unit again in order to regulate this device of operational condition.

The running of this device is as follows: charging mechanism [2] moves to cultivation vessel the mixing head [8] of opening outlet.At this operation middle slide plate [4] is helpful.Mixing head [8] adds the necessary component that stub bar [71], [72] add the control requirement on storage unit [61] and the storage unit [62].In cultivating vessel, solidify component and make the developing medium solidification.Transport unit [5] transmission is cultivated vessel at the most to guiding device [10], finishes transplanting herein.Transport unit under connecing [11] transmission is cultivated vessel and is entered following operation.

Can short stay when cultivating vessel and be delivered to transport unit [5], (short stop) transplants at its surface seeding is possible.In this case, do not need multidirectional liner [10].

Transport unit [5] or [11] advance these cultivation vessel to their device of sterilization sealing after transplanting.According to cultivating the beneath possibility that can pause of vessel, so from installing [13] with on the PVC paillon foil depressor ware at cylinder puopulsion unit [13].Heater strip [14] cuts the book sheet and under the influence of thermal treatment unit [19] relatively-high temperature, the paillon foil of this culture vessel is tightened up on top then.Conditions for sterilization is realized in this case fully.

Another preferred version of the stopper of sealed plastic system, in this case, dosage unit [16] promotes the top of stopper to container, and they are pressed onto on the container by tightness system [18] herein on the surface.

Take out the culture vessel of sealing bottom from device in sterilized space, and tightness system adopts plastics stopper and paillon foil too herein.The sealing stopper has porous materials or other gas-pervious tuft material is made in this situation, and fibrous root is according to the requirement of following operation steps.

Claims (9)

1, a kind of Microculture device for plant, comprise a sterilized space and the cultivation vessel of sterilizing, it is characterized in that being included in the storage unit [1] that is used to advance the charging mechanism [2] of cultivating vessel is housed in the sterilized space, be used for cultivating vessel and guarantee disinfectant device [3] at storage unit [1], be assemblied in below the charging mechanism [2] or the transport unit [5] around it, be used to adorn the storage unit [61] of the cultivation component of sterilizing, be used to adorn the storage unit [62] of the curing component of sterilizing, add stub bar [71,72] at least one and storage unit [61] and storage unit [62] are connected, what mixing head [8] and band were opened outlet adds stub bar [71,72] at least one is connected, be used to guarantee storage unit [61] and the temperature required thermostat [91 of storage unit [62], 92] and the device that is used to cultivate vessel sterilization sealing.

2, device according to claim 1, the device that it is characterized in that cultivating vessel sterilization sealing comprise be equipped in being used on the transport unit [5] adorn the sealing paillon foil supporter [12], be used to advance the cylinder puopulsion unit [13] of sealing paillon foil and be in and be used for the heater strip [14] of cutting sealing paillon foil and preferably be a thermal treatment unit [19] near cylinder puopulsion unit [13].

3, device according to claim 1 and 2, the device that it is characterized in that cultivating vessel sterilization sealing comprises storage unit [15], the dosage unit [16] that is used to advance sealing plug that is used to adorn sealing plug and is used to guarantee sealing plug disinfectant device [17] and if desired, is used for sealing plug is fixed on the tightness system of cultivating on the vessel [18].

4,, it is characterized in that being used to guarantee that disinfectant device [3,17] comprises a ultraviolet lamp according to any described device in the claim 1 to 3.

5,, it is characterized in that thermostat [91,92] surrounds at least one unit that comprises the group that adds in the stub bar [71,72] at least one and mixing head [8] according to any described device in the claim 1 to 4.

6, device according to claim 1 is characterized in that the transport unit [5] that adopts has the protrusion surface.

7, device according to claim 1 is characterized in that adopting tube unit to be used for mixing head [8] and adds stub bar [71,72] at least one is connected.

8, according to any described device in the claim 1 to 7, its feature is included in the slide plate [4] below the charging mechanism [2].

9, according to any described device in the claim 1 to 8, its feature is included in the multidirectional liner [10] of transport unit [5] outlet and transport unit of following below [11] and the liner [10] that is preferably with the protrusion surface connect.

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