CN86201206U - Device for drawing trace spectrum point sample from thin layer - Google Patents
Device for drawing trace spectrum point sample from thin layer Download PDFInfo
- Publication number
- CN86201206U CN86201206U CN 86201206 CN86201206U CN86201206U CN 86201206 U CN86201206 U CN 86201206U CN 86201206 CN86201206 CN 86201206 CN 86201206 U CN86201206 U CN 86201206U CN 86201206 U CN86201206 U CN 86201206U
- Authority
- CN
- China
- Prior art keywords
- nut
- sample
- extraction chamber
- screw rod
- push plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses the device for drawing trace point samples from thin-layer chromatogram of sample ingredients in the thin-layer chromatogram analysis of the crude drug, Chinese patent drug samples and the similar samples. The device is composed of a screw rod-nut advancing mechanism, an injector, a trace extraction chamber, a capillary point sample head with an air-stream narrow lane and an electrical heating plate. The injector is impelled by the advancing mechanism to make the extraction solvent in the injector enter slowly the trace extraction chamber, and continuously extracts the ingredients of samples in the extraction chamber. The extraction solution is formed into fine solution stream through the capillary of the point sample head and is diverted onto the point sample points of the thin-layer chromatogram plate. The extraction solvent is volatilized to dryness naturally, or is evaporated to dryness by the heating of a heating plate, or is blown to dryness by introducing compressed gas.
Description
The trace that the utility model relates to sample composition in a kind of analytical chemistry, particularly crude drug and Chinese patent drug sample composition extracts the thin-layer chromatography spot sample device.
The quality research of crude drug and Chinese patent drug and control are by the qualitative and quantitative analysis of its some composition of sample is finished.Thin-layered chromatography is a kind of qualitative and quantitative analysis method commonly used.The common operating process of this method is, the peek gram is to the sample of tens of grams through pulverizing, at glass container, or in the composition characteristic is selected per sample the special-purpose extraction apparatus such as Sha Shi extraction apparatus and volatile oil distiller, its composition of extraction solvent extraction that uses tens of milliliters to arrive hundreds of milliliters.Filter extract.Concentrated extracting solution.In case of necessity the extract evaporation is done, converted to the solution of another kind of solvent.With glass capillary get concentrate count microlitre to tens of microlitre points on the chromatographic sheet test agent.Then, launch evaluation and mensuration with thin-layer chromatogram.Above-mentionedly be extracted into this process of chromatographic sheet point sample, exist manual operations loaded down with trivial details, tediously long, inefficiency from sample composition; Sample and extraction solvent load are big; Easily cause during concentrated extracting solution and extract the pollution of solvent laboratory environment; And operating process is difficult for problems such as standardization.
The purpose of this utility model is to provide a kind of trace to extract the thin-layer chromatography spot sample device, and sample composition extraction and one step of chromatographic sheet point sample operating process in crude drug, Chinese patent drug and other similar sample thin-layered chromatography analyses are finished automatically.
The purpose of this utility model is achieved in that the cylindrical stainless steel trace extraction chamber that two ends have into or export, its import is connected with the outlet of thin plastic pipe with the syringe of a thin bore low capacity, and its outlet is connected with an end of a stainless steel capillary with thin plastic pipe.This stainless steel capillary is vertical with the thin-layer chromatography board plane, and its other end is aimed at and pressed close to point of sample on the chromatographic sheet.Sample through pulverizing is seated in the micro-extraction chamber, extracts solvent and places syringe.Use a screw-and-nut propulsive mechanism that is driven by slow speed motor, it is vertical with screw axis that nut is fixed on its plane, and on the push plate that can only move along this axis direction, the push plate planar top the plunger of syringe.When screw rod rotated, nut drove push plate along axis direction, and the pushing syringe plunger makes the extraction solvent enter micro-extraction chamber very lentamente then, and sample composition is extracted continuously.The sample composition extract that comes out from micro-extraction chamber flows to stainless steel capillary.Form thin liquid stream through this kapillary, after transferring on the chromatographic sheet point of sample continuously, extracting solvent can be according to its volatility, allowing it volatilize naturally does, or the heating of heating plate is set under chromatographic sheet its evaporation is done, or the gas channel of a diameter less than point of sample is set around kapillary, and to introduce pressure gas it is dried up, sample composition then is attached on the point of sample.
The utility model makes the staff save the sample extraction in the thin-layer chromatographic analysis, and multistep manual operationss such as the filtration of extract and concentrated and chromatosheet point sample are finished high efficiency automatically from being extracted into whole one step of process of point sample.With conventional method relatively, the utility model also has following advantage: sample and to extract solvent load few only needs 1 to 5 milligram of sample and less than 1 milliliter extraction solvent; The staff is few with extraction solvent touch opportunity; The sample that is suitable for the different physicochemical characteristics of various tools; The good reproducibility of operation, easily standardization.In addition, apparatus cost is low, and is simple to operate.
The concrete structure of the utility model embodiment device is provided by the following drawings:
Fig. 1. device construction figure
Fig. 2. micro-extraction chamber (14) longitudinal sectional view
Fig. 3. sampling head (16) longitudinal sectional view
Fig. 4. electric boiling plate (20) longitudinal sectional view
Fig. 5. push plate (6) view
Referring to Fig. 1.SD-4.5 type servomotor (1) adopts the connection in series-parallel capacitance phase-shifting circuit, and rotating speed is controlled at 1-4 rev/min with the method for directly regulating the control winding voltage.Motor rotary shaft by connector (2) and frame at support plate (3) and the S8 (9-1) * 1 screw rod (4) link, screw rod (4) length is between 10-15 centimetre.Nut (5) is blocked by push plate (6).When motor (1) drove screw rod (4) rotation, nut (5) was done axially-movable, promoted push plate (6), the plunger (7) of pushing syringe (8) then.Injector length is between 5.5-11 centimetre, and capacity is corresponding between the 0.35-0.70 milliliter.Like this, the extraction solvent in the syringe (8) is 0.5 millimeter through internal diameter, and external diameter is that 1.5 millimeters vinyl tube (10) slowly enters stainless steel trace extraction chamber (14), and the interior sample of chamber (23) is extracted continuously.The diameter of chamber (23) is between the 1.5-2.5 millimeter, and length is between the 10-20 millimeter.The extract that come out (14) from the extraction chamber, by 0.5 millimeter of internal diameter, the vinyl tube that external diameter is 1.5 millimeters (11) enters the stainless steel capillary (18) of sampling head (16).The internal diameter of kapillary (18) is between the 0.2-0.4 millimeter, and external diameter is corresponding between the 0.4-0.6 millimeter.Extract forms thin liquid stream by kapillary (18), transfers on chromatographic sheet (19) point of sample pressed close to the kapillary top.Extract solvent waving except that there being three kinds of modes available on point of sample: volatilization is done naturally; Dry up around the pressure gas in the air-flow passageway (28) of kapillary (18) with importing in the sampling head (16).The thickness in air-flow passageway (28) is between the 0.1-0.2 millimeter, and length is not less than 15 millimeters; To the chromatographic sheet heating, its evaporation is done by electric boiling plate (20).The nickel filament (31) of heating plate (20) connects 110 volts of power supplys, and power is 100W, and directly heating surface (area) (HS is 25 square centimeters.The temperature in chromatographic sheet (19) point of sample zone is 75 ℃ of-85 ℃ of scopes.
The concrete structure of trace extraction chamber (14) is seen Fig. 2.Extraction chamber's liquid in-out mouth is connected with vinyon conduit (10) and (11) respectively by nut (22) extruded polyethylene plastic seal ring (21).
The concrete structure of sampling head (16) is seen Fig. 3.Vinyl tube with micro-extraction chamber (14) outlet links to each other is connected with sampling head body (27) and stainless steel capillary (18) by nut (26) extruded polyethylene O-ring seal (25).Stainless steel capillary (18) passes the gas channel and the syringe needle (17) of body (27), and forms one around this gas capillaceous passageway (28) with syringe needle (17).Distance between the outlet of syringe needle (17) and kapillary (18) outlet can be regulated by screwing syringe needle (17).One side of sampling head body has inlet for pressurised gas.
The concrete structure of heating plate is seen Fig. 4.Nichrome wire (31) is clipped in thickness and is between 0.5 millimeter the aluminium sheet (29) and sheet asbestos (34) on mica sheet (32).Insulating effect electrifies for mica sheet (30) and (33).
The operating process of the utility model device is as follows: take by weighing the sample of 1-5 milligram through pulverizing.One little glass wool bulb stopper is extracted solvent outlet one end in micro-extraction chamber (14).The sample of packing into and taking by weighing, vibration makes it to fill out closely a little.One little glass wool bulb stopper is extracted solvent inlet one end in micro-extraction chamber (14).Connect and import and export plastic tube (10) and (11).Draw the extraction solvent with syringe (8), fill with plastic tube (10) and extraction chamber (14).Then, with syringe (8) frame at support plate (9-1) with (9-2).Regulate support (12) and make the point of sample plane of chromatographic sheet (19) and distance to 0.1 millimeter between kapillary (18) outlet.If need heating evaporation to extract solvent, connect the power supply of heating plate (20), allow chromatographic sheet (19) preheating a few minutes.If need to dry up solvent, regulate airshed with pressure gas.Actuating motor (1) chooses rotating speed.Extracting the point sample process promptly begins.Process is closed motor power and heating plate power supply after finishing, or cuts off air-flow, takes off chromatosheet (19).
Use the present embodiment device, can handle two samples simultaneously.
Claims (9)
1, sample composition in the analytical chemistry, particularly crude drug and Chinese patent drug sample composition trace extracts the thin-layer chromatography spot sample device, this device is by servomotor (1), the propulsive mechanism of forming by drive screw (4), nut (5) and push plate (6), syringe (8), stainless steel trace extraction chamber (14), sampling head (16) and electric boiling plate (20) are formed, and it is characterized in that:
1. screw rod (4) is passed to push plate (6) by nut (5) with power, by the plunger (7) of push plate (6) pushing syringe (8), supplies with and extracts solvent;
2. extraction chamber (14) are miniature, and volume is between 17.5～50.0 cubic millimeters;
3. sampling head (16) has stainless steel capillary (18) and around this air-flow capillaceous passageway (28);
4. electric hot plate (20) keeps the temperature of chromatographic sheet (19) between 75 ℃～85 ℃.
2, according to the described device of claim 1, the pitch that it is characterized in that screw rod (4) is 1 millimeter, and rotating speed is that per minute 1～4 changes.
3, according to the described device of claim 1, it is characterized in that push plate (6) have two parallel, the height and the muscle of nut (5) consistency of thickness, in order to clamp nut (5), center parallel lines along two parallel muscle, push plate (6) has a breach down, the width of this breach and height, respectively with the diameter of screw rod (4) and screw rod (4) frame at support plate (3) and the apex height (9-1) equate, like this, push plate (6) can upwards be smoked, and separates with nut (5) with screw rod (4) and takes off, and is convenient to load and unload syringe (8) and allows nut (5) hand-reset.
4, according to the described device of claim 1, the length that it is characterized in that syringe (8) is between 55～110 millimeters, and capacity is corresponding between 0.35～0.7 milliliter.
5, according to the described device of claim 1, it is characterized in that micro-extraction chamber (14) chamber (23) is elongated cylindrical, chamber (23) diameter is between 1.5～2.5 millimeters, and chamber (23) length is between 10～20 millimeters.
6, according to claim 1 or 5 described devices, it is characterized in that the import and export place of micro-extraction chamber (14), link to each other with (11) with vinyl tube (10) respectively by nut (22) extruded polyethylene plastic seal ring (21).
7, according to the described device of claim 1, the internal diameter that it is characterized in that stainless steel capillary (18) is between 0.2～0.4 millimeter, and external diameter is corresponding between 0.4～0.6 millimeter.
8, according to claim 1 or 7 described devices, it is characterized in that thickness around the air-flow passageway (28) of kapillary (18) between 0.1～0.2 millimeter, length is not less than 15 millimeters.
9, according to the described device of claim 1, nichrome wire (31) power that it is characterized in that electric boiling plate (20) is 100W, directly heating surface (area) (HS is 25 square centimeters, connects 110 volts of AC power, and the temperature that keeps chromatographic sheet (19) is between 75 ℃～85 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86201206 CN86201206U (en) | 1986-03-14 | 1986-03-14 | Device for drawing trace spectrum point sample from thin layer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 86201206 CN86201206U (en) | 1986-03-14 | 1986-03-14 | Device for drawing trace spectrum point sample from thin layer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN86201206U true CN86201206U (en) | 1986-10-22 |
Family
ID=4805014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 86201206 Ceased CN86201206U (en) | 1986-03-14 | 1986-03-14 | Device for drawing trace spectrum point sample from thin layer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN86201206U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103712956A (en) * | 2014-01-15 | 2014-04-09 | 中国科学院化学研究所 | Microarray sample application device |
| CN104965047A (en) * | 2014-10-13 | 2015-10-07 | 中国药科大学 | Novel planar chromatography sample application instrument and step repeated sample application method |
| CN106908563A (en) * | 2015-12-23 | 2017-06-30 | 中美华世通生物医药科技（武汉）有限公司 | Spotting system and method |
| CN110160835A (en) * | 2019-05-24 | 2019-08-23 | 深圳市前海微升科学股份有限公司 | A kind of method and device thereof of quantitative microsampling and transmission |
-
1986
- 1986-03-14 CN CN 86201206 patent/CN86201206U/en not_active Ceased
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103712956A (en) * | 2014-01-15 | 2014-04-09 | 中国科学院化学研究所 | Microarray sample application device |
| CN103712956B (en) * | 2014-01-15 | 2016-05-11 | 中国科学院化学研究所 | A kind of microarray spot sample device |
| CN104965047A (en) * | 2014-10-13 | 2015-10-07 | 中国药科大学 | Novel planar chromatography sample application instrument and step repeated sample application method |
| CN106908563A (en) * | 2015-12-23 | 2017-06-30 | 中美华世通生物医药科技（武汉）有限公司 | Spotting system and method |
| CN106908563B (en) * | 2015-12-23 | 2024-01-05 | 中美华世通生物医药科技(武汉)股份有限公司 | Sample application system and method |
| CN110160835A (en) * | 2019-05-24 | 2019-08-23 | 深圳市前海微升科学股份有限公司 | A kind of method and device thereof of quantitative microsampling and transmission |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6297499B1 (en) | Method and apparatus for electrospray ionization | |
| Burger et al. | Headspace gas analysis: Quantitative trapping and thermal desorption of volatiles using fused-silica open tubular capillary traps | |
| Lovins et al. | Liquid chromatography-mass spectrometry. Coupling of a liquid chromatograph to a mass spectrometer | |
| EP1697736B1 (en) | Device for sample preparation | |
| WO2022052855A1 (en) | Combined device of liquid mass spectrometry sampling and electrospray | |
| CN86201206U (en) | Device for drawing trace spectrum point sample from thin layer | |
| CN109709198A (en) | A kind of on-line preconcentration method of Capillary Electrophoresis | |
| CN204989098U (en) | Capillary electrophoresis and electrochromatography plug -in components | |
| US6475437B1 (en) | Method and system for preparing samples for gas chromatography | |
| CN108303484B (en) | Liquid chromatography fraction collector and using method thereof | |
| CN107228911A (en) | Ultrashort chromatogram microtrabeculae for biological sample quick separating and preparation method thereof | |
| JPS6197567A (en) | Sample preprocessor | |
| Dowty et al. | Automated gas chromatographic procedure to analyze volatile organics in water and biological fluids | |
| Morita et al. | Silver nitrate impregnation of preparative silica gel columns for liquid chromatography | |
| EP1010468A1 (en) | Improved method and apparatus for electrospray ionization | |
| DE2044702C3 (en) | Method for expelling the components of a sample mixture from a storage column | |
| CN203842349U (en) | Pressurizing device for nanofiber solid-phase extraction column | |
| CN202083680U (en) | Evaporative light scattering detector | |
| CN102621253A (en) | On-line sample preparation device for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) | |
| CN2700880Y (en) | Solid-phase micro extraction apparatus | |
| CN107303538B (en) | A kind of biology molecule separating equipment and separation method | |
| CN2294472Y (en) | Automatic thin layer sample counter | |
| CN2128385Y (en) | Negative pressure thermal conduction tester for capillary gas chromatography | |
| CN219091165U (en) | Integrated manual solid-phase microextraction device | |
| CN201149564Y (en) | Quantitative adjustable abstraction purifier for pesticide residue |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CE01 | Termination of patent right | ||
| CE01 | Termination of patent right |