CN85201392U - Separating funnel-type centrifuge tube - Google Patents

Separating funnel-type centrifuge tube Download PDF

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Publication number
CN85201392U
CN85201392U CN 85201392 CN85201392U CN85201392U CN 85201392 U CN85201392 U CN 85201392U CN 85201392 CN85201392 CN 85201392 CN 85201392 U CN85201392 U CN 85201392U CN 85201392 U CN85201392 U CN 85201392U
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CN
China
Prior art keywords
centrifuge tube
piston
liquid
hole
duct
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Ceased
Application number
CN 85201392
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Chinese (zh)
Inventor
高祖泉
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Shandong University
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Shandong University
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Filing date
Publication date
Application filed by Shandong University filed Critical Shandong University
Priority to CN 85201392 priority Critical patent/CN85201392U/en
Publication of CN85201392U publication Critical patent/CN85201392U/en
Ceased legal-status Critical Current

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Abstract

The utility model discloses a liquid-separating funnel type centrifuge tube whose bottom is provided with a horn-shaped hole, and the hole way is interdicted by a transverse piston. A hole way is arranged on the transverse center of the piston, and the two hole ways are communicated with each other, by rotating the piston appropriately, in order to form a funnel; the liquid can flow out of the centrifuge tube. The former method that the liquid is sucked from the top is improved, the operation of the experiment is simplified, and the quality is improved.

Description

Separating funnel-type centrifuge tube
The utility model is a kind of centrifuge tube.
In the experiment in fields such as chemistry, chemical industry, biology, medicine, agricultural and producing, usually extract certain material with method of extraction repeatedly.Material after the extraction need quicken with centrifuge, makes various different specific weights, mutual exclusive liquid layering.Because used centrifuge tube is smooth circle of bottom lock formula or flat shape at present, the liquid after the layering normally takes out respectively with hydrocone type siphon method.This traditional method not only slowly but also inconvenient.And the liquid of drawing by avoiding is polluted by another layer liquid, often can not exhaust required liquid fully, thereby cause waste, causes yield low.Moreover, centrifugal owing to extracting repeatedly, take out a sample and will repeat unexpectedly more than ten time, it is very bored that the staff generally feels.
In order to overcome these shortcomings, the invention provides a kind of funneling centrifuge tube.This centrifuge tube combines the characteristics of centrifuge tube and two kinds of utensils of separatory funnel, makes the liquid behind the score layer, can directly flow out from the funnel of centrifuge tube bottom, has improved operating efficiency and quality.
Below be that description of drawings of the present invention and specific embodiment: Fig. 1 are the sectional structure charts of the funneling centrifuge tube manufactured by glass or transparent corrosion-resistant duroplasts, the bottom center of centrifuge tube among the figure (1) has a smooth tubaeform duct (3), and duct (3) are blocked by porose lateral taper piston (2).Top land has a square opening, and when with stainless steel bottle opener (5) rotating piston (2), the duct of piston (2) central authorities is connected with the duct (3) of centrifuge tube (1) bottom, forms a funnel.(rubber stopper (4) can be used for blocking the duct (3) of centrifuge tube (1)).
When using funneling centrifuge tube, earlier piston (2) is placed the position in blocking-up centrifuge tube duct (3).Then liquid is poured in the pipe, centrifugal make the liquid layering after, with 90 ° of stainless steel bottle opener (5) rotating pistons, (dotted line indication among the figure) duct in the piston is connected with centrifuge tube duct (3), liquid is slowly outflow just.Liquid has flowed when lower floor, and when middle level liquid began to arrive pipe end aperture, rotating piston was closed the duct immediately.Just obtain lower floor's liquid of the overwhelming majority, and not by the middle level liquid contamination.Continue after also can similarly obtain middle level liquid, supernatant liquid.
For example, when the Biochemistry Experiment chamber prepares DNA (DNA), extract repeatedly, to remove the dawn white matter in the dna solution with chloroform one isoamyl alcohol.When carrying out this work, at first chloroform one isoamyl alcohol and DNA salting liquid are vibrated in triangular flask, then, pour centrifuge tube into, through 3000 rev/mins after centrifugal 15 minutes, lamination appears in liquid.The DNA salting liquid of upper strata for needing, the middle level is a sex change dawn white matter, lower floor is chloroform one isoamyl alcohol.When taking out supernatant liquid,, both be afraid of that agitated liquid caused the sex change dawn to pollute in vain, be afraid of again that suction did not cause damage only, therefore wanted significant care with the careful absorption of siphon pipe according to old law.In fact usually be that you can't have both at the same time, or contaminated or lose more.If with novel separatory funnel formula centrifuge tube, just can overcome this shortcoming fully, mixed liquor can be directly in the mid-shaking table vibration of centrifuge tube, centrifugal in the rearmounted centrifuge, open the duct, bottom with the piston bottle opener then, reclaim earlier chloroform one isoamyl alcohol, it is white to discard the medial degeneration dawn again, the upper strata DNA salting liquid that stays needn't take out, and directly adds chloroform one isoamyl alcohol that reclaims and extracts once more.Through reprocessing several times, just can be the very fast dawn white matter be removed is clean like this.
Obviously, Novel centrifugal tube has many advantages, in experiment, uses it not need solution is transferred in other container, just can directly extract in centrifuge tube, has improved operating efficiency; Owing to can obtain the liquid of getting of the overwhelming majority, reduce the loss of utility, thereby improved yield; And, use it with operator's wish, to select easily, to collect arbitrary layer liquid; In addition, reduced pollution, the quality of purifying substances is improved greatly.
Therefore this is a kind of novel centrifugal pipe that is worthy to be popularized, and for all laboratory and factories that centrifuge is arranged, all will obtain an advantage from the present invention.
Must note several problems during use:
1, in order to prevent the seepage phenomenon, should between piston and piston hole, be coated with last layer vaseline.
2, owing to centrifugal speed increases, and the centrifugal force that produces may cause piston outwards to slide.Therefore, piston hole should be oblique, and when centrifugal, the top of piston is made progress, and like this, the centrifugal component of generation makes piston tightr on the contrary.If the top of piston is downward, then can cause piston to move outward, the phenomenon of leakage appears.
Need at pipe bottom outlet place rubber choker block beyond the Great Wall when 3, centrifugal, because the pressure of centrifuge tube and centrifugal sleeve, the rubber choker block is filled in tighter and tighter, with the unlikely outflow of assurance liquid in pipe.

Claims (3)

1, a kind of separatory funnel formula centrifuge tube, it is a main body 1 with glass or transparent corrosion-resistant duroplasts, it is characterized in that a smooth tubaeform duct (3) is arranged at the bottom, a tapered piston (2) is laterally deposited in centrifuge tube (1) bottom, and hole (3) are blocked.
2, centrifuge tube according to claim 1 is characterized in that the lateral center of tapered piston has a duct, and when with stainless steel bottle opener (5) rotating piston (2), hole (3) can be connected with piston hole.
3, it is characterized in that described centrifuge tube according to claim 1 or 2, duct (3) collar extension can stop up with a rubber choker block (4).
CN 85201392 1985-04-02 1985-04-02 Separating funnel-type centrifuge tube Ceased CN85201392U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 85201392 CN85201392U (en) 1985-04-02 1985-04-02 Separating funnel-type centrifuge tube

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 85201392 CN85201392U (en) 1985-04-02 1985-04-02 Separating funnel-type centrifuge tube

Publications (1)

Publication Number Publication Date
CN85201392U true CN85201392U (en) 1986-01-01

Family

ID=4797472

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 85201392 Ceased CN85201392U (en) 1985-04-02 1985-04-02 Separating funnel-type centrifuge tube

Country Status (1)

Country Link
CN (1) CN85201392U (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102728129A (en) * 2012-06-20 2012-10-17 瓮福(集团)有限责任公司 Solid-liquid separator for electronic chemical production
CN103842001A (en) * 2011-12-29 2014-06-04 阿梅迪卡株式会社 Integrated kit for separating blood and concentrating prp and method for extracting prp using same
CN106861786A (en) * 2017-04-11 2017-06-20 中国农业科学院麻类研究所 A kind of centrifuge tube for dividing liquid

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103842001A (en) * 2011-12-29 2014-06-04 阿梅迪卡株式会社 Integrated kit for separating blood and concentrating prp and method for extracting prp using same
CN102728129A (en) * 2012-06-20 2012-10-17 瓮福(集团)有限责任公司 Solid-liquid separator for electronic chemical production
CN106861786A (en) * 2017-04-11 2017-06-20 中国农业科学院麻类研究所 A kind of centrifuge tube for dividing liquid
CN106861786B (en) * 2017-04-11 2022-05-20 中国农业科学院麻类研究所 Centrifugal tube capable of separating liquid

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