CN2931487Y - High pressure cell culture in vitro apparatus - Google Patents
High pressure cell culture in vitro apparatus Download PDFInfo
- Publication number
- CN2931487Y CN2931487Y CN 200620097266 CN200620097266U CN2931487Y CN 2931487 Y CN2931487 Y CN 2931487Y CN 200620097266 CN200620097266 CN 200620097266 CN 200620097266 U CN200620097266 U CN 200620097266U CN 2931487 Y CN2931487 Y CN 2931487Y
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- China
- Prior art keywords
- pressure
- cam
- electric motor
- utility
- pressurized vessel
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- Expired - Fee Related
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Abstract
The utility model discloses a device comprising a pressure container, an electric motor, a corrugated pipe and a cam. A one-way adjustable pressure reducing valve is arranged on the pressure container and the cam is connected with the output shaft of the electric motor through a connecting bar. The cam bottom is provided with the corrugated pipe on which an air inlet valve is arranged. The corrugated pipe is connected with the pressure container through a pipeline and the pressure container is further provided with a pressure gage. The cam is elliptically-shaped. The electric motor is connected with an adjusting device which is used for adjusting the electric motor rotational speed. The attaching portion of the pressure container cap and the container body is provided with a sealing ring. The utility model can simulate the machinery mechanics change which is borne by the cell in the high blood pressure pathological state and simulate how the hydrostatic pressure change affects cell function. The utility model has important significance for researching cell shape and functional changes caused by the change of hydrostatic pressure, and for studying vessel construction and the mechanism of high blood pressure target organ damage caused by high blood pressure. The utility model achieves simple structure, practical property, low cost and good effect.
Description
Technical field
The utility model relates to a kind of cell culture apparatus, relates in particular to a kind of high pressure isolated cells culture apparatus.
Background technology
Vascular endothelial cell, vascular smooth muscle cell, kidney mesangial cell bear the hydrostaticpressure (wall pressure that blood produces vessel wall in vivo, just usually said arteriotony), distraction force (vasoconstriction and diastole make cell be subjected to tractive and produce), vascular endothelial cell also bear shear-stress (frictional force that blood flow and cell surface produce).The influence that different mechanical forces changes cellular function is different.Adopt the pressure kettle of the transformation of the way to cultivate porcine aorta endotheliocyte and Human umbilical vein endothelial cells or adopt pressure assembly to cultivate the kidney of rats mesangial cell both at home and abroad at present, all be the variation pair cell form of simulation hydrostaticpressure and the influence of function, but container inner pressure all is a continuous and constant pressure in these models, and the hydrostaticpressure that produces along with heart contraction and diastole with intra-arterial pressure fluctuates, and this is misfitted in the body situation.
Summary of the invention
The purpose of this utility model is: overcome the above-mentioned deficiency of prior art, but the variation of the mechanics of machinery (hydrostaticpressure) that a kind of analog cell born under hypertension pathology state, simple in structure, convenient and practical, high pressure isolated cells culture apparatus that cost is low, effective are provided.
The utility model is realized by following scheme: be made of pressurized vessel, electric motor, corrugated tube, cam.Pressurized vessel is provided with unidirectional adjustable compression release valve, and cam connects with motor output shaft through a connecting rod, and the cam bottom is provided with corrugated tube, and intake valve is set on the corrugated tube, and corrugated tube links to each other with pressurized vessel by pipeline, also is provided with tensimeter on the pressurized vessel.
Cam described in the utility model is ellipse garden shape.
Electric motor described in the utility model connects a speed regulator, so that regulate motor speed.
The lid of pressurized vessel described in the utility model and container body junction surface are provided with sealing-ring.
The relative normal atmosphere of vessel internal pressure power described in the utility model is 0-200mmHg, and the pressure difference in each period of motion pressurized vessel is 30-40mmHg.
But the variation of the mechanics of machinery that the utility model analog cell is born under hypertension pathology state, the simulation hydrostaticpressure changes the influence of cellular function, electric motor continues to rotate makes corrugated tube correspondingly shrink and diastole by being rotated in of cam in each swing circle, gas is sent in the container when corrugated tube is compressed, the entire container internal volume increases when the corrugated tube diastole, thereby container inner pressure descends, pressure in the container is contracted with corrugated tube easypro and in certain scope, fluctuate, it is poor that adjusting corrugated tube compression degree can be regulated container inner pressure, thereby more approaching at the body state.Owing to adopted intake valve and air outlet valve simultaneously, make that the gas in the container can constantly be replaced, guaranteed in the container gas formation, temperature, humidity, pH value in normal range, keep the cell required environment of growing.The machine-processed significant of vascular remodeling that hypertension causes and hypertension target organ damage inquired in the form that the utility model causes because of hydrostaticpressure changes for the research cell, the research of changes of function.The utility model is simple in structure, practical, cost is low, effective.
Description of drawings
Accompanying drawing is the utility model example structure synoptic diagram.
Embodiment
Below in conjunction with accompanying drawing the utility model is elaborated.
The utility model is made of seal-off pressure container 1, electric motor 2, corrugated tube 3, ellipse garden shape cam 4.Pressurized vessel 1 is provided with unidirectional adjustable compression release valve 6, and its lid is provided with sealing-ring 9 with container body junction surface.Cam 4 connects with electric motor 2 output shafts through a connecting rod, and electric motor 2 connects a speed regulator 8, so that regulate motor speed.Cam 4 bottoms are provided with corrugated tube 3, and intake valve 5 is set on the corrugated tube 3, and corrugated tube 3 links to each other with pressurized vessel 1 by pipeline, also are provided with tensimeter 7 on the pressurized vessel 1.The relative normal atmosphere of pressurized vessel 1 internal pressure is 0-200mmHg, and the pressure difference in each period of motion pressurized vessel 1 is 30-40mmHg.
Principle of work of the present utility model is: the motor drives cam ceaselessly rotates in the original place on the corrugated tube top, because the axle that cam rotates is different to the distance of taking turns the edge at different directions, and distance title major axis farthest, the title minor axis that distance is the shortest.Because axle center and connecting rod connect together, so the position in axle center (being relative height) is constant.When cam from short-axis direction to the long axis direction rotary course, axle center to the length of taking turns the edge increases gradually, simultaneously owing to the shaft core position of cam is fixed, so cam is compressed corrugated tube gradually, the check valve that is positioned at the corrugated tube bottom this moment is in closing condition, and gas is compressed and enters encloses container; Shortening gradually from major axis axle center to distance of cam edge when short-axis direction rotates when cam equally, the degree of cam compress bellows alleviates gradually, the self-dependent elasticity of corrugated tube and diastole, and gas enters corrugated tube.Rotate a circle at a swing circle convexity repeating query major axis-minor axis-long axis direction, make corrugated tube do once to shrink and the diastole campaign in vertical direction.So electric motor rotates a cam and rotates a circle, the corrugated tube above-below direction is done once the vertical motion of contracting of relaxing.Gas enters and is pressed into the pressure that makes in the container in the container and raises when corrugated tube is compressed, and when corrugated tube corresponding increase of volume in the container during in diastole, thereby pressure descends.Corrugated tube container inner pressure difference when compression and diastole fluctuates between 30-40mmHg.When container inner pressure surpassed preset value, gas was discharged by vent valve.Container inner pressure is monitored by tensimeter.Container inner pressure can be regulated by regulating vent valve inner spring compression degree simultaneously, tighten reducing valve on the vent valve, weak spring compression degree is increased, spring stretches that to wash the required pressure of valve open big more, container inner pressure is raise, and pressure parameter can be regulated arbitrarily between 0-200mmHg.Because corrugated tube is compression and diastole constantly, container inner pressure is constantly raise and reduce, the periodical change that simulation intra-arterial hydrostaticpressure produces with heart contraction and diastole, and by regulating motor speed adjustment cam speed, and then the frequency of adjusting pressure change, the variation of simulation heart rate under situation is simultaneously by regulating the gap (cam that replacing varies in size) of corrugated tube compression, diastole degree, regulate container inner pressure fluctuation range up and down, regulate pulse pressure difference.When using, places in whole device CO
2In the incubator, satisfy the needs that cell is grown with the gas that guarantees to enter in the pressurized vessel.
Claims (5)
1, a kind of high pressure isolated cells culture apparatus, it is characterized in that: constitute by pressurized vessel (1), electric motor (2), corrugated tube (3), cam (4), pressurized vessel (1) is provided with unidirectional adjustable compression release valve (6), cam (4) connects with electric motor (2) output shaft through a connecting rod, cam (4) bottom is provided with corrugated tube (3), intake valve (5) is set on the corrugated tube (3), and corrugated tube (3) links to each other with pressurized vessel (1) by pipeline, also is provided with tensimeter (7) on the pressurized vessel (1).
2, high pressure isolated cells culture apparatus according to claim 1, it is characterized in that: cam (4) is ellipse garden shape.
3, high pressure isolated cells culture apparatus according to claim 1 and 2 is characterized in that: electric motor (2) connects a speed regulator (8), so that regulate motor speed.
4, high pressure isolated cells culture apparatus according to claim 1 and 2 is characterized in that: pressurized vessel (1) lid is provided with sealing-ring (9) with container body junction surface.
5, high pressure isolated cells culture apparatus according to claim 2 is characterized in that: the relative normal atmosphere of pressurized vessel (1) internal pressure is 0-200mmHg, and the pressure difference in each period of motion pressurized vessel (1) is 30-40mmHg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620097266 CN2931487Y (en) | 2006-06-16 | 2006-06-16 | High pressure cell culture in vitro apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620097266 CN2931487Y (en) | 2006-06-16 | 2006-06-16 | High pressure cell culture in vitro apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2931487Y true CN2931487Y (en) | 2007-08-08 |
Family
ID=38347987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620097266 Expired - Fee Related CN2931487Y (en) | 2006-06-16 | 2006-06-16 | High pressure cell culture in vitro apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2931487Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329724A (en) * | 2011-09-22 | 2012-01-25 | 哈尔滨医科大学 | System and method for testing influence of pressure on cell growth |
| CN103232937A (en) * | 2013-04-18 | 2013-08-07 | 刘傥 | Cell pressure test system |
| CN113755432A (en) * | 2020-07-17 | 2021-12-07 | 上海我武干细胞科技有限公司 | Stem cell culture method |
-
2006
- 2006-06-16 CN CN 200620097266 patent/CN2931487Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329724A (en) * | 2011-09-22 | 2012-01-25 | 哈尔滨医科大学 | System and method for testing influence of pressure on cell growth |
| CN103232937A (en) * | 2013-04-18 | 2013-08-07 | 刘傥 | Cell pressure test system |
| CN113755432A (en) * | 2020-07-17 | 2021-12-07 | 上海我武干细胞科技有限公司 | Stem cell culture method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |