CN2909243Y - Quick investigating device of flavatin B - Google Patents
Quick investigating device of flavatin B Download PDFInfo
- Publication number
- CN2909243Y CN2909243Y CN 200620097322 CN200620097322U CN2909243Y CN 2909243 Y CN2909243 Y CN 2909243Y CN 200620097322 CN200620097322 CN 200620097322 CN 200620097322 U CN200620097322 U CN 200620097322U CN 2909243 Y CN2909243 Y CN 2909243Y
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- afb
- aflatoxin
- color filter
- utility
- model
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The utility model relates to a rapid measurement device for aflatoxin B 1, including an optical measuring device A, a signal amplification device B, a data processing device C and a measurement output device D. the optical measuring device A consists of a light source (1), a color filter (2), a focus (3), a slit (4), a sample pool (5), a color filter (6) and a detector (8). Therein, the data processing device has the standard curve data about the quantitative determination of the aflatoxin B 1. The effectiveness of the utility model is that: 1. The utility model is specialized in rapid measurement of aflatoxin B 1 with good performance and accurate result and could realize rapid quantitative measurement of aflatoxin B 1 in various food and agricultural products; 2. The utility model has a small size, is easy to carry and could be read directly to facilitate the use at the scene and enhance the practicality and easy operability combined with the property of optical design optimization and systematic integration.
Description
Technical field
The utility model relates to a kind of AFB
1Rapid measurement device is especially for aflatoxin B1 rapid measurement device in agricultural product and the food.
Background technology
Since nineteen sixty, turkey aflatoxin (Aflatoxin) nosotoxicosis took place in Britain, the human knowledge is extremely strong to the toxicity of aflatoxin, pollution range to food, agricultural product and goods thereof etc. is wide, and is very big to the potential threat of human life's health, causes the great attention of countries in the world.Guarantee peanut, soybean, corn, rice and edible oil consumption safety, promote the production and processing and the foreign exchange earning of high quality agricultural products, improve agricultural products in China quality safety level and international and domestic competitive power, enlarge the export of farm produce, the guarantee public's consumption safety and healthy all has great importance.
Aflatoxin is a class is infected the toxicity secondary metabolite of oil plant, grain or the generation of other food by aspergillus flavus (Aspergillus flavus Link) and aspergillus parasiticus (Aspergillus parasiticusSpeare) a general name, basic structure is formed (cumarin) by one two furan nucleus and a coumarin, principal ingredient comprises six kinds, i.e. B1, B2, G1, G2, M1.Because the chemical stability of aflatoxin B1 and the popularity that exists in agricultural product and in the food thereof will thoroughly be removed very difficulty or even impossible with aflatoxin B1 fully from agricultural product and food.Pollution control along with science and technology development and aflatoxin should be reduced in the content in agricultural product and the food as far as possible.Therefore the detection for aflatoxin B1 in food and the agricultural product seems very important, and existing examination of aflatoxin method mainly comprises thin layer chromatography (TLC), biological detection method, instrumental method and immunoassay etc.Instrument and equipment by means of large-scale costliness is measured aflatoxin, mainly comprises high performance liquid chromatography (HPLC) method, fluorescence opacimeter method, ultraviolet spectrophotometry, micro-column fluorescence reaction method, oscilloscopic polarography, mass spectroscopy, nuclear magnetic resonance method, X ray derivatization method etc.As publication number in 2005 was CN1673746 fluorescence opacimeter method, and the outstanding feature of these class methods is that accuracy of measurement is higher, good reproducibility, but need Large-Scale Precision Instrument and Equipment, numerous, the consuming time length of program, expense height.
Summary of the invention
The utility model is the deficiency that exists at above-mentioned prior art, and provide a kind ofly can realize the aflatoxin B1 rapid measurement device.
The utility model is that the technical scheme that problem adopted of the above-mentioned proposition of solution is: it includes optical measuring device A, the signal amplifying apparatus B, data processing equipment C and the measurement output unit D that are made up of light source 1, color filter 2, focusing body 3, slit 4, testing sample pond 5, color filter 6, detector 8, wherein, in the described data processing equipment AFB is housed
1The typical curve data of quantitative measurement.
According to technique scheme, described data processing equipment comprises an A/D analog to digital converter and microprocessor, wherein an AFB
1The bent data line of the standard of quantitative measurement is contained in the microprocessor.
According to technique scheme, described measurement output unit comprises printer and display.
According to technique scheme, described microprocessor also is connected with display, touch keyboard, printer, serial ports, clock, ROM, RAM.
According to technique scheme, also be connected with shadow shield 7 between described color filter 6 and the detector 8.
Design concept of the present utility model is according to fluorescence spectrophotometry ultimate principle and AFB
1The characteristic of molecular fluorescence adopts technological means such as modern light, mechanical, electrical, computing machine, agricultural product to be measured is carried out pre-service, fast purifying, fluorescence strengthen the reactant that the back generates, and quantizes fast and directly expresses, and improves AFB
1The test duration is shortened in the specificity and the sensitivity that detect, is suitable for AFB
1Quantitatively fast detecting realizes AFB
1AFB is convenient in quantitative fast survey technology materialization
1The practical application of speed survey technology.
The beneficial effect that the utility model is obtained is: 1, the utility model only is exclusively used in AFB
1Fast measuring, instrument performance is good, measurement result is accurate, can realize the quantitative determination of aflatoxin B1 content in various agricultural product and the food; 2, the utility model is by optimizing light path design, and systematization is integrated, and volume is less, is convenient for carrying also directly reading, is convenient to use at the scene, has strengthened practicality and ease for operation.
Description of drawings
Fig. 1 is an AFB
1The principle sketch of rapid measurement device;
Fig. 2 is the schematic diagram of aflatoxin B1 rapid measurement device.
Embodiment
Below in conjunction with accompanying drawing embodiment of the present utility model is described further.
As Fig. 1, as Fig. 2, the utility model comprises optical measuring device A, signal amplifying apparatus B, and data processing equipment C measures output unit D.Measuring AFB
1The time, testing sample is packed in the testing sample pond 5, the light beam that light source 1 sends filters through color filter 2, after focusing on body 3 optically focused, passes slit 4, pass the testing sample pond again, testing sample in the testing sample pond absorbs light and produces fluorescence, and the fluorescence of generation filters through color filter 6 and arrives detector 8, and detector is according to the corresponding electric signal of the strong and weak generation of fluorescence, electric signal after amplifier B amplifies again among the Input Data Process C, its after treatment with AFB
1After the typical curve contrast of quantitative measurement, just exportable AFB
1As a result, just can directly read the result by display then, can also it be printed with printer.
Described optical measuring device A includes light source 1, color filter 2, focuses on body 3, slit 4, testing sample pond 5, color filter 6, detector 8.Can be connected with shadow shield 7 between color filter 6 and detector 8, shadow shield is extracted out when measuring, when time spent not inserts shadow shield and covers light.Light source 1 adopts the 200W xenon lamp, and high-precision integrated stable voltage circuit is used in power supply, can guarantee the stability of instrument itself.Structurally, light source 1 can guarantee that three-dimensional coordinate is regulated and locking; Color filter 2 adopts 365nm ± 5nm color filter, and its effect is in order to obtain high-quality monochromatic light; Focus on body 3 and adopt convex lens, effect is in order to obtain high-quality parallel beam; Parallel beam is eliminated the parasitic light of part by slit then; The testing sample pond is used for placing the testing sample of various agricultural product and food; For reducing noise and improving sensitivity, color filter 6 adopts 440nm ± 5nm color filter; Detector adopts end window photomultiplier (head-on PMT), light signal can be changed into electric signal.Taked in the utility model optical measuring device heat insulation, ventilate and reduce the heat radiation measure to keep the relatively stable of temperature; For reducing reflection of light and spuious, whole light paths are handled the aluminium manufacturing with special surface in the utility model simultaneously, and the surperficial blackness of light path element is very high.
What described signal amplifying apparatus B adopted is the TLC4502 integrated operational amplifier with characteristics such as self calibration, low noise, high precision.
Described data processing equipment C comprises: an A/D analog to digital converter and a microprocessor, be equipped with AFB in the microprocessor
1The typical curve of quantitative measurement can carry out that necessary processing comprises and AFB to all measurement data
1Typical curve compares.Wherein analog to digital converter can adopt at a high speed 12 comparison A/D converter AD574A one by one of monolithic, microprocessor can adopt the AT89C55WD microprocessor, and the microprocessor rear end also is connected with display, touch keyboard, printer, serial ports, clock, ROM, RAM.
Described measurement output unit D comprises display and printer.Display can use dot matrix graph lcd module LCM12864ZK, can directly show aflatoxin B1 content (μ g/Kg) by Chinese character; Printer adopts SP-A40 Chinese character mini-printer, and measurement result is directly exported by printer prints.
Claims (5)
1, a kind of AFB
1Rapid measurement device, it is characterized in that it comprises optical measuring device A, signal amplifying apparatus B, data processing equipment C and a measurement output unit D who is made up of light source (1), color filter (2), focusing body (3), slit (4), testing sample pond (5), color filter (6), detector (8), is equipped with AFB in the described data processing equipment
1The typical curve data of quantitative measurement.
2, by the described AFB of claim 1
1Rapid measurement device is characterized in that described data processing equipment comprises an A/D analog to digital converter and microprocessor, wherein an AFB
1The typical curve data of quantitative measurement are contained in the microprocessor.
3, by the described AFB of claim 1
1Rapid measurement device is characterized in that described measurement output unit comprises printer and display.
4, by the described AFB of claim 2
1Rapid measurement device is characterized in that described microprocessor also is connected with display, touch keyboard, printer, serial ports, clock, ROM, RAM.
5, by the described AFB of claim 1
1Rapid measurement device is characterized in that also being connected with shadow shield (7) between color filter (6) and detector (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620097322 CN2909243Y (en) | 2006-06-20 | 2006-06-20 | Quick investigating device of flavatin B |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620097322 CN2909243Y (en) | 2006-06-20 | 2006-06-20 | Quick investigating device of flavatin B |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2909243Y true CN2909243Y (en) | 2007-06-06 |
Family
ID=38128460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620097322 Expired - Lifetime CN2909243Y (en) | 2006-06-20 | 2006-06-20 | Quick investigating device of flavatin B |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2909243Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183570A (en) * | 2011-02-14 | 2011-09-14 | 北京软测科技有限公司 | Realization method for improving blood detection accuracy and detector |
| CN103116024A (en) * | 2012-11-30 | 2013-05-22 | 中国农业科学院油料作物研究所 | Application and method of anti-aflatoxin universal monoclonal antibody 1C11 in aflatoxin B1 fluorescence quenching |
| CN103217528A (en) * | 2012-11-30 | 2013-07-24 | 中国农业科学院油料作物研究所 | Non-labeled immunization analysis method for detecting content of aflatoxin B1 |
-
2006
- 2006-06-20 CN CN 200620097322 patent/CN2909243Y/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183570A (en) * | 2011-02-14 | 2011-09-14 | 北京软测科技有限公司 | Realization method for improving blood detection accuracy and detector |
| CN103116024A (en) * | 2012-11-30 | 2013-05-22 | 中国农业科学院油料作物研究所 | Application and method of anti-aflatoxin universal monoclonal antibody 1C11 in aflatoxin B1 fluorescence quenching |
| CN103217528A (en) * | 2012-11-30 | 2013-07-24 | 中国农业科学院油料作物研究所 | Non-labeled immunization analysis method for detecting content of aflatoxin B1 |
| CN103116024B (en) * | 2012-11-30 | 2014-04-09 | 中国农业科学院油料作物研究所 | Application and method of anti-aflatoxin universal monoclonal antibody 1C11 in aflatoxin B1 fluorescence quenching |
| CN103217528B (en) * | 2012-11-30 | 2014-04-09 | 中国农业科学院油料作物研究所 | Non-labeled immunization analysis method for detecting content of aflatoxin B1 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20070606 |
|
| EXPY | Termination of patent right or utility model |