CN2884737Y - Fluorescent imaging scanner - Google Patents
Fluorescent imaging scanner Download PDFInfo
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- CN2884737Y CN2884737Y CN 200620039860 CN200620039860U CN2884737Y CN 2884737 Y CN2884737 Y CN 2884737Y CN 200620039860 CN200620039860 CN 200620039860 CN 200620039860 U CN200620039860 U CN 200620039860U CN 2884737 Y CN2884737 Y CN 2884737Y
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- light source
- fluorescence
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- scanning
- secondary light
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- 238000012632 fluorescent imaging Methods 0.000 title 1
- 238000012634 optical imaging Methods 0.000 claims abstract description 16
- 238000003384 imaging method Methods 0.000 claims abstract description 14
- 238000000799 fluorescence microscopy Methods 0.000 claims abstract description 9
- 230000010365 information processing Effects 0.000 claims abstract description 4
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Images
Abstract
The utility model provides a scanner with the function of fluorescence imaging, which can scan fluorescence marker to images and merge the images with the non-fluorescent letters of the objects and the scanned pictorial images to realize the complete image information. The scanner can not only comprises first light source and optical imaging system, photoelectricity conversion system, information processing system and movable machinery components that can complete general draft scanning and imaging, and it also comprises second light source for exciting the fluorescent marker to send fluorescence. The first light source is used to scan, to obtain the outline of the scanned objects and display the non-fluorescent letters and logo images, and then to activate the second light source so that the fluorescent marker sends fluorescence, which is scanned to obtain fluorescence marker image and merge the two scanned results, thereby achieving a complete image information.
Description
Technical field
The utility model relates to a kind of scanner that has the fluorescence imaging function concurrently, and this scanner can not only scan common manuscript, and can realize fluorescence imaging.This utility model can be used for the biochemical analysis of molecular biology, medicine and other fields, particularly fluoroscopic examination analysis, as: have fluorescently-labeled protein, DNA, RNA in the running gel, the detection of the organic molecule on the thin-layer chromatography TLC plate.
Background technology
Electrophoresis is that biology field is used for one of effective method of large biological molecules such as protein isolate, DNA, RNA, the result who separates makes various big molecules be ribbon in gel to distribute, but these bands dyed after Direct observation or at its fluorescence of irradiating and detecting of specific light.Thin-layered chromatography (TLC) is to detect one of effective method of organic molecule, be widely used in the drug ingredient analysis, it is with the TLC plate each micromolecule to be separated earlier equally, develops the color then and detects, and be the most frequently used coloration method wherein with ultraviolet irradiation generation visible fluorescence.In the biological and chemical technical field, also has multiple detection to film or dull and stereotyped visible material or fluorescent material.A kind of method that realizes these detections is exactly an imaging analysis, promptly obtains the image of the object of observation with light imaging apparatus, then its color, intensity etc. is analyzed.
Along with the development of digital technology, digital camera is integrated in the various imaging devices, as: the TLC board detector of main flow and running gel imaging system in the market.This type systematic is generally a casing, and white light or fluorescence excitation light source are arranged in the casing, adorns a digital camera at the top of casing, is used to take the TLC plate or the gel that lie in bottom half.Since camera need certain focusing from, device height usually about 0.5 meter, so volume is bigger.The another kind of product manuscript scanner of digital technology in fact also is a kind of imaging system owing to adopted the micro lens array, greatly reduce focusing from, therefore can be used for developing small-sized portable imaging system.
Because common manuscript scanner is a product very ripe on the market, this paper is in its operation principle of this simple declaration: with uniform linear white light source scanning contribution, with reverberation (scanning reflection original text, as: picture) or transmitted light (scanning transmission original text, as: (system that market occurs comprises: photoelectrical coupler CCD photographic negative) to be imaged onto photo-translating system through one group of reflection/transmission mirror, contact-type image sensor CIS or LIDE, photomultiplier PMT etc.), again the signal of telecommunication is converted into digital data transmission in computer by analog/digital converter, realizes that by software image shows at last.Manuscript scanner on the market is reflective the most common with platform, Fig. 1 is its structural representation, when on the translation slide bar 2 of moving assembly 3 (it comprises a part and some mechanical components of optical imaging system, photo-translating system, A/D converter, electric-control system) in housing 1 when mobile, white wire light source 4 inswept manuscript planes on the moving assembly, its reverberation is converted to the signal of telecommunication by optical imaging system 5, and this signal is used for computer information processing after digital translation and image shows.
Utilize common manuscript scanner to be mainly used in the scanning imagery of visible material, although can scan those materials that under visible light (as: 635nm visible light) irradiation, can send fluorescence with it, but owing to also contain other a large amount of veiling glares in the light source, serious interference can't be practical.
In biological or chemical is analyzed, be used for the more employing ultraviolet lights of light source (as: 254,306,312,365nm ultraviolet light) of fluorescence excitation, even laser.Therefore the white light source of common manuscript scanner is changed into the light source of specific wavelength, just can realize fluorescent scanning.Patent CN00125286.0 describes, and " ultraviolet scanner " that be used to detect anti-fake mark adopts this method just.The image in fluorescent material zone can only be obtained to contain with this ultraviolet scanner, and literal, mark and the pattern etc. of other regional non-fluorescence can't be shown.As when carrying out the TLC analysis, the analyst marks information such as sample title, numbering, concentration, point sample position with pencil usually on the TLC plate.Imaging system in the market generally is being filled into these information on the image with software behind the fluorescence imaging, but this way has been run counter to the principle of record primitiveness, is easy to generate marking error.As for the point sample position, it is a starting point of determining heterogeneity migration distance in the sample, is the key parameter of differentiating various compositions.These non-fluorescence information can not obtain with above-mentioned ultraviolet scanner.
Moreover reflective common manuscript scanner adopts linear light sorurce scanning, and for finishing large-area scanning at short notice, light source moves very fast, and promptly the time of staying of light source on sweep object is very short.In biochemical analysis, need prolongation to excite light application time sometimes to obtain more distinct image, realize and present scanner is very difficult.
Based on the problem of above existence, this paper proposes a kind of scanner that has the fluorescence imaging function concurrently of practicality.
Summary of the invention
Scanner described in the utility model comprises two parts: (1) common manuscript scanning system: white light source (first light source), optical imaging system, photo-translating system, the information processing system mechanically moving member etc. of unifying; (2) secondary light source.The first of scanner is used to finish the scanning imagery of whole contours of objects and non-fluorescence labelling wherein, secondary light source is used to activate the fluorescent material on the sweep object, after obtaining fluorescence, utilize first's (except white light source) to obtain the image of fluorescent material.
In order to increase the adjustable range of secondary light source light application time, secondary light source is an area source.Area source defines with respect to line source, and this paper refers to form the light source of the above irradiating width of 2mm on sweep object.The working method of secondary light source fluorescence excitation has two kinds: static excite and scanning excites.
When adopting static mode of excitation, secondary light source is independent of common manuscript scanning system, has independently mains switch or time control circuit.This light source can cover whole sweep object zone, behind the irradiation certain hour, starts common manuscript scanning system (first light source keeps closed condition) fluorescence is carried out image scanning.Adopt this mode of excitation, irradiation time can unrestrictedly be regulated.
When adopting the scanning mode of excitation, secondary light source can with optical imaging system with respect to the same moved further of sweep object, when a line had been swept on the overall optical plane, the fluorescence that sends on this line was caught by optical imaging system, is embodied as picture.As mentioned above, light application time increases with the width of area source, and in conjunction with the scanning translational speed, the illumination firing time can reach several minutes again.
In the actual mechanical process, obtain complete image information and will carry out twice scanning at least, start for the first time first light source and obtain object outline and non-fluorescence labelling image, utilize for the second time the secondary light source irradiation to excite and obtain visible fluorescence and imaging, result with twice scanning merges then, obtains complete object information.
Since fluorescence intensity a little less than than exciting light many, in order to obtain preferable imaging effect, tackle following three factors and control.At first, the wave spectrum of excitation source should be controlled at narrower scope, promptly reduces the interference of veiling glare.The second, sweep object and optical imaging system should be in the black box of lucifuge, to reduce the interference of environment light source.The 3rd, minimizing enters the reflection or the transmitted light of the secondary light source of optical imaging system as far as possible.
Secondary light source can be single wavelength light source, also can be the combination of a plurality of light sources, and multiple excitation source can be provided simultaneously.When design, secondary light source also can adopt standalone module or unit, so that change light sources with different wavelengths.Secondary light source also can have independently mains switch or time controller.
Because the ordinary flat scanner uses white light source, objective table is generally simple glass, and this glass is bigger to UV Absorption, and the transmissivity of ultraviolet light is low.In order to save the energy, obtain better scanning effect, simple glass can be changed into ultraviolet and the good material (as: quartz glass) of visible transmission tool; Perhaps remove the simple glass that is used to support sweep object, and use support instead,, enter to prevent dust simultaneously at first light source with increase the cloche or the plastic film of saturating visible light above the optical imagery.
The purpose of this invention is to provide a kind of scanner that has the fluorescence imaging function concurrently, it not only can obtain the image of fluorescent material (or mark), and can the common non-fluorescence labelling of scanning imagery, and both are merged into complete image.
Another object of the present invention provides the scanning imaging system that a kind of fluorescence-activation time can regulate by several minutes in the several seconds on a large scale.
Description of drawings
Fig. 1 is the schematic diagram of platform-type common manuscript scanner
Fig. 2 is one embodiment of the invention (static mode of excitation) schematic diagrames
Fig. 3 is the schematic side view of one embodiment of the invention (static mode of excitation)
Fig. 4 is one embodiment of the invention (scanning mode of excitation) schematic diagrames
1 is housing among the figure, and 2 is slide bar, and 3 is moving assembly, and 4 is first light source, and 5 is optical imaging system, and 6 is secondary light source, and 7 is objective table.
Embodiment
The embodiment schematic diagram that Fig. 2 shows is the scanner of the static mode of excitation of an employing, and Fig. 3 is its end view, and it is improved on the basis of common manuscript scanner, and its main feature is to have increased a secondary light source 6, is the 254nm ultraviolet light in the present embodiment.Below just with the operating process of the imaging process of TLC plate explanation fluorescent scanning instrument.The TLC plate is placed on the objective table 7, and working face down.Open first light source 4 and carry out the scanning first time, obtain the contour images of TLC plate and top visible indicium (as: point sample position, sample title, numbering etc.) thereof.Behind the end of scan, close white light source 4, start secondary light source 6, make the whole TLC plate of this light source irradiation; Observe the TLC plate profile diagram that obtains with white light scanning by computer software simultaneously, from scheming to obtain the point sample position and the mobile frontal line that moves mutually of sample, zone between selected point sample position and the migration forward position, this zone is the working region of fluorescent material.Shine a period of time (several seconds was by several minutes) on request at secondary light source after, carry out the scanning second time, this time the zone of scanning is selected working region, the scanning back first time.White light source keeps closed condition when carrying out scanning the second time, therefore only shows the fluorescent material image.The information that obtains with scanning is for the second time replaced the same area information of scanning for the first time at last, and other zones keep, and then obtain complete TLC plate image, have shown information such as original sample title, numbering on this image.
In the present embodiment, secondary light source 6 adopts and tilts to place, and parallel with white light source, and its purpose mainly is to reduce the volume of scanner housing.In scanning for the second time, the fluorescence that send in edge line (dotted arrow indication among Fig. 3) zone of excitation light irradiation is caught by optical imaging system, and with moving of moving assembly 3, fluorescence imaging can be realized in whole irradiated area.Obtain fluorescent material relative concentration information as need, remove and need guarantee that exciting light is a uniformity, and the TLC plate should be dull and stereotyped state on objective table, therefore the most handy smooth cover plate is pressed in the TLC plate on the objective table.
Because secondary light source places the housing peripheral position, so an advantage of present embodiment is to change secondary light source easily, to meet the different needs.
Fig. 4 is the scanner schematic diagram of another embodiment, and arrow is the scanning moving direction among the figure.Compare with the plain scan instrument, it has increased secondary light source 6, and white light source is placed on the downstream of optical imaging system simultaneously.During practical operation, start 4 scannings of first light source earlier, this moment, secondary light source 6 was closed, and obtained contours of objects and non-fluorescence labelling; Can select the fluorescence labelling zone after the end; Start secondary light source scanning (this moment, first light source was closed) then, as shown in the figure, secondary light source is in the upstream of optical imaging system, and after secondary light source scanning fluorescence labeling inspired fluorescence, the fluorescence that optical imaging system 5 is following closely caught the range of linearity, edge of irradiated region was embodied as picture.Moving assembly 3 can slide on the slide bar in the housing 12 among the figure, realizes scanning.
Among this embodiment, the width of light source scalable of scanning mode of excitation scanner, adjustable range from the millimeter level to several centimetres.A kind of simple implementation is that a plurality of line sources are arranged in parallel, and the quantity that line source is opened is many more, and light source is wide more; Another implementation method is to increase the light path door in the light source outlet, changes irradiating width by the size that changes the door slit.Adopt the less light source of volume during the scanning mode of excitation, therefore can further reduce the volume of scanner.
More than two embodiment all adopted reflective scanning mode, it is the same side that light source and optical imaging system are in sweep object, if with the be placed in both sides of sweep object of light source and optical system, can realize transmission-type scanning, this scan mode can be used for light transmission sweep objects such as gel.Utilize paper feed type manuscript scanner, increase secondary light source, can realize the fluorescent scanning imaging equally by above technical scheme.
Above-mentioned preferred embodiment and design only are structure and the usages that illustrates novel scanner, are not to be used for limited field of the present invention, and the content that this utility model comprised is defined by claims.
Claims (6)
1. scanner that has the fluorescence imaging function concurrently, it comprises first light source, optical imaging system, photo-translating system, the information processing system that can the realize common manuscript imaging mechanically moving member of unifying, and it is characterized in that it also comprises to be used for the secondary light source that the fluorescence excitation material sends fluorescence.
2. scanner according to claim 1, secondary light source wherein are the single wavelength ultraviolet light of wavelength 254nm, 365nm or other wavelength.
3. scanner according to claim 1, secondary light source wherein are the specific wavelength visible light of wave-length coverage at 400nm-800nm.
4. scanner according to claim 1, secondary light source wherein is the multi-wavelength composite light source of two or more single wavelength light source combinations.
5. scanner according to claim 1, secondary light source is wherein fixed with respect to sweep object, and its irradiation area can cover whole prescan object.
6. scanner according to claim 1, secondary light source wherein and optical imaging system are can be synchronization-moving with respect to sweep object.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620039860 CN2884737Y (en) | 2006-03-01 | 2006-03-01 | Fluorescent imaging scanner |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620039860 CN2884737Y (en) | 2006-03-01 | 2006-03-01 | Fluorescent imaging scanner |
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| Publication Number | Publication Date |
|---|---|
| CN2884737Y true CN2884737Y (en) | 2007-03-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200620039860 Expired - Fee Related CN2884737Y (en) | 2006-03-01 | 2006-03-01 | Fluorescent imaging scanner |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101446868B (en) * | 2007-11-28 | 2010-10-06 | 汉王科技股份有限公司 | Scanning tablet |
| CN101964857A (en) * | 2009-07-22 | 2011-02-02 | 威海华菱光电有限公司 | Image reading device |
| WO2016096078A1 (en) * | 2014-12-19 | 2016-06-23 | Merck Patent Gmbh | Method and device for optically sensing a chromatographic specimen |
| CN110826352A (en) * | 2018-08-10 | 2020-02-21 | 维蒂克影像国际无限责任公司 | Long range bar code scanning by coherent light conversion |
-
2006
- 2006-03-01 CN CN 200620039860 patent/CN2884737Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101446868B (en) * | 2007-11-28 | 2010-10-06 | 汉王科技股份有限公司 | Scanning tablet |
| CN101964857A (en) * | 2009-07-22 | 2011-02-02 | 威海华菱光电有限公司 | Image reading device |
| WO2016096078A1 (en) * | 2014-12-19 | 2016-06-23 | Merck Patent Gmbh | Method and device for optically sensing a chromatographic specimen |
| US10578595B2 (en) | 2014-12-19 | 2020-03-03 | Merck Patent Gmbh | Method and device for the optical scanning of a chromatographic sample |
| CN110826352A (en) * | 2018-08-10 | 2020-02-21 | 维蒂克影像国际无限责任公司 | Long range bar code scanning by coherent light conversion |
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| GR01 | Patent grant | ||
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Patentee address after: 200063 room 450, No. 1506 Cao Yang Road, Shanghai, Putuo District Patentee address before: 200333 Shanghai city Putuo District Meichuan Road 255 Lane 9, Room 202 |
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| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |