CN2844899Y - Experiment device for generation of simulated microorgan/macromolecular membrane - Google Patents
Experiment device for generation of simulated microorgan/macromolecular membrane Download PDFInfo
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- CN2844899Y CN2844899Y CN 200520124862 CN200520124862U CN2844899Y CN 2844899 Y CN2844899 Y CN 2844899Y CN 200520124862 CN200520124862 CN 200520124862 CN 200520124862 U CN200520124862 U CN 200520124862U CN 2844899 Y CN2844899 Y CN 2844899Y
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Abstract
The utility model discloses an experiment device for simulating the generation of microorganism / macromolecule membranes. The utility model is characterized in that both ends of a flow pool bed under a simulating microbiological corrosion environment are provided with a circulating experiment liquid outlet and a circulating experiment liquid inlet; the pool bed surface between the outlet and the inlet is provided with at least one installation slot hole which is used for a research electrode; the electrode surface of the research electrode has the same height or is slightly lower than the pool bed surface; the bed body is provided with a sealing bin and a bin cover of the circulating experiment liquid flow pool bed. The utility model can simultaneously simulate the preparation of multiple microbial membranes in parallel, and obtain the regularity data of generating the microorganism / macromolecule membranes. The utility model can simulate the flow rate of surface current liquid of various metal facilities, the microorganism can attach on the electrode surface of the research electrode, and generates microorganism / macromolecule membranes which are formed by initial condition membranes, and single layer membranes and ripe membranes at different stages. The utility model can carry out microscopic observation and scanning Kelvin measurement in an online, and can be taken out to carry out the characterization analysis and observation, such as SEM, ESEM, etc.
Description
Technical field
The utility model relates to the improvement of microbiological corrosion monitoring equipment, specifically is a kind of experimental provision that microorganism/big molecular film generates of simulating.It is to generate microbial film at the experiment lab simulation, and carries out the experimental provision of microbiological corrosion research, and it belongs to microbiology and corrosion science crossed technical
Technical background
The microbiological corrosion of prior art mainly occurs in the various liquid environments, as the various metallic facilities surface in the industrial condensing water conduit, in sewage treatment plant, rivers and lakes and the briny environment.Exist a large amount of microorganisms in these environment, they at first adhere at material surface, if environmental baseline is fit to, these microorganisms just begin continuous metabolism breeding, secrete a large amount of exocytosis things (EPS) simultaneously.Material surface forms with EPS these exocytosis things is skeleton with firm the sticking to of microorganism, various microorganisms and metabolic product distribution thereof microorganism/big molecular film wherein, though wherein be water more than 90%, but this layer microorganism/big molecular film can change the physics and the chemical environment of microbial film/metal surface in the part, thereby brings out the generation of metal surface local corrosion.Microbiological corrosion is a very complicated process, so want its mechanism is studied, must in the laboratory, the process to microbiological corrosion simulate, wherein the simulation of microorganism/big molecular film is a very important step, only simulate the microorganism/big molecular film of different stages of development, could in the laboratory, detail knowledge be arranged the microbiological corrosion process.So the foundation of microorganism/big molecular simulation film has significance for the generation development of explaining microbiological corrosion.
Summary of the invention
The utility model purpose is: design a kind of equipment that can simulate the microorganism/big molecular film of different phase growth in the laboratory, promptly simulate the experimental provision that microorganism/big molecular film generates.This device will have " the condition film " at simulation generation initial stage, monofilm, the function of the microorganism that different phases such as ripe film form/big molecular film; Have simple in structurely, easy to install, can take out and carry out that phenetic analysis is observed and online function of carrying out microexamination and scanning survey.
The purpose of this utility model is realized by following technical scheme, has developed a kind of experimental provision that microorganism/big molecular film generates of simulating, and it comprises: circulation experiment liquid, thermopnore pond and research electrode.Flow cell bed two ends at simulation microbiological corrosion environment, circulation experiment liquid entry and exit are set, on the pond bed bed surface between the Liang Kou, be provided with the installation slotted eye of one piece of research electrode at least, electrode surface Shunping County of this research electrode in or a little less than this pond bed bed surface, place, lathe bed space above this pond bed bed surface is provided with the gas-tight silo and the Cang Gai thereof of circulation experiment liquid flow cell bed.
The installation slotted eye of described research electrode, its aperture equal or are slightly larger than the aperture of research electrode, in this slotted eye sealing study of installing electrode by the screw thread cock with its sealing and fixing in this slotted eye; The hole count that this slotted eye is provided with on the bed bed surface of pond is; 1-8 piece.
Described gas-tight silo, its lathe bed cell wall and gas-tight silo lid by this bed bed surface top, pond is formed; The gas-tight silo that on this lathe bed cell wall, has been threaded lid, this Cang Gai or make for transparent organic glass, or make for stainless steel.
Described gas-tight silo lid, it also is equipped with the mechanism that carries out microexamination probe and scanning measurement system real-time photography, online.
Described circulation experiment liquid entry and exit, it is interconnected by conduit and peristaltic pump between this outer end, entry and exit except with the gas-tight silo two ends of flow cell bed communicate.
Described circulation experiment liquid, itself or contain bacterium culture medium solution for the aseptic seawater dilution of simulation microbiological corrosion environment; Itself or for including polysaccharide, the biological macromolecule solns of nucleic acid and protein; Itself or be the solution of the industrial condensate water environment of simulation, itself or be the solution of simulation sewage environment, itself or be simulation river, the solution of lake or briny environment.
Advantage of the present utility model is: owing at the flow cell bed two ends of simulation microbiological corrosion environment, circulation experiment liquid entry and exit are set, be provided with the installation slotted eye of one piece of research electrode at least on the pond bed bed surface between the Liang Kou.Promptly slotted eye should be installed, its aperture equals or is slightly larger than the aperture of research electrode, in this slotted eye sealing study of installing electrode by the screw thread cock with its sealing and fixing in this slotted eye; The hole count that this slotted eye is provided with on the bed bed surface of pond is; 1---8 pieces.Therefore the preparation of a plurality of microbial films of parallel simulation simultaneously is so that obtain the regular data that microorganism/big molecular film generates.Since this research electrode electrode surface Shunping County in or a little less than this pond bed bed surface, again because the conduit and the peristaltic pump that between outer end, circulation experiment liquid entry and exit, are provided with, can simulate in industrial condensing water conduit, the flow rate of the various metallic facility surface flow liquid in sewage treatment plant, rivers and lakes and the briny environment, make microorganism can generate " the condition film " at initial stage in adhering to of electrode surface of research, monofilm, the microorganism that different phases such as ripe film form/big molecular film.Because place, the lathe bed space above this pond bed bed surface is provided with the gas-tight silo and the Cang Gai thereof of circulation experiment liquid flow cell bed.This Cang Gai or make for transparent organic glass, or make for stainless steel.This Cang Gai also is equipped with the mechanism that carries out microexamination probe and scanning measurement system real-time photography, online, and this device can onlinely carry out microexamination and scanning Kelvin measures, and also can take out and carry out the phenetic analysis observation, as SEM, ESEM etc.
Accompanying drawing and embodiment thereof
Fig. 1 is the experimental provision structure cut-open view of simulation microorganism/big molecular film generation.
Fig. 2 is the experimental provision vertical view of simulation microorganism/big molecular film generation.
Referring to Fig. 1,2 a kind of experimental provisions of simulating microorganism/big molecular film generation of making, it comprises: circulation experiment liquid 7, thermopnore pond 8 and research electrode 3.Flow cell bed 8 two ends at simulation microbiological corrosion environment, circulation experiment liquid 7 entry and exit 1,6 are set, on pond bed 8 bed surfaces between the Liang Kou, be provided with the installation slotted eye 4 of one piece of research electrode 3 at least, electrode surface Shunping County of this research electrode 3 in or a little less than these pond bed 8 bed surfaces, place, lathe bed space above these pond bed 8 bed surfaces is provided with the gas-tight silo 9 and the storehouse lid 5 thereof of circulation experiment liquid 7 flow cell beds.
The installation slotted eye 4 of described research electrode, its aperture equal or are slightly larger than the aperture of research electrode 3, in this slotted eye 4 sealing study of installing electrodes 3 by screw thread cock 4 ' with its sealing and fixing in this slotted eye 4; The hole count that this slotted eye 4 is provided with on pond bed 8 bed surfaces is; 1---8 pieces.
Described gas-tight silo 9, its lathe bed cell wall and gas-tight silo lid 5 by this pond bed 8 bed surfaces top is formed; The gas-tight silo that on this lathe bed cell wall, has been threaded lid 5, this storehouse lid 5 or make for transparent organic glass, or make for stainless steel.
Described gas-tight silo lid 5, it also is equipped with the mechanism's (not drawing among the figure) that carries out microexamination probe and scanning measurement system real-time photography, online.
Described circulation experiment liquid entry and exit 1,6, it is interconnected by conduit and peristaltic pump (not drawing among the figure) between these 1,6 outer ends, entry and exit except with gas-tight silo 9 two ends of flow cell bed 8 communicate.
Described circulation experiment liquid 7, itself or contain bacterium culture medium solution for the aseptic seawater dilution of simulation microbiological corrosion environment; Itself or for including polysaccharide, the biological macromolecule solns of nucleic acid and protein; Itself or be the solution of the industrial condensate water environment of simulation, itself or be the solution of simulation sewage environment, itself or be simulation river, the solution of lake or briny environment.
Specific embodiment:
The state that sulphate reducing bacteria (SRB) adheres at stainless steel surfaces in the research seawater.SRB extracts from the ooze of coastal waters, Qingdao, and anaerobism is preserved after the purifies and separates.Get 10 milliliters and contain bacterium culture medium injection one liter of aseptic seawater (filtration of 0.2 micron membranes), aseptic seawater is connected with the entry and exit 1,6 of this experimental provision by conduit.Seawater cycles through peristaltic pump to carry out.This experimental provision comprises the installation slotted eye 4 of six research electrodes.The circulation of circulation experiment seawater in the time of 3,5,7,10 hours, is taken out an electrode respectively 1,2 successively, carries out microscopic examination after formaldehyde fixed, coloring agent dyeing.Change in time by observing comparison quantity of SRB in this seawater.In addition, can also scan Kelvin probe scanning to electrode.By the contact of surperficial SRB position feature and micro zone corrosion electric potential feature, the variation characteristic of metal surface micro zone corrosion electric potential in the research microbial adhesion process.
Those of ordinary skill in the art can understand, and in protection domain of the present utility model, makes amendment for the foregoing description, and it all is possible adding and replacing, and it does not all exceed protection domain of the present utility model.
Claims (6)
1, a kind of experimental provision of simulating microorganism/big molecular film generation, it comprises: circulation experiment liquid, thermopnore pond and research electrode, it is characterized in that: at the flow cell bed two ends of simulation microbiological corrosion environment, circulation experiment liquid entry and exit are set, on the pond bed bed surface between the Liang Kou, be provided with the installation slotted eye of one piece of research electrode at least, electrode surface Shunping County of this research electrode in or a little less than this pond bed bed surface, place, lathe bed space above this pond bed bed surface is provided with the gas-tight silo and the Cang Gai thereof of circulation experiment liquid flow cell bed.
2, according to the described experimental provision of simulating microorganism/big molecular film generation of claim 1, it is characterized in that: the installation slotted eye of described research electrode, its aperture equals or is slightly larger than the aperture of research electrode, in this slotted eye sealing study of installing electrode by the screw thread cock with its sealing and fixing in this slotted eye; The hole count that this slotted eye is provided with on the bed bed surface of pond is; 1--8 piece.
3, according to the experimental provision that the described simulation of claim 1 microorganism/big molecular film generates, it is characterized in that: described gas-tight silo, its lathe bed cell wall and gas-tight silo lid by this bed bed surface top, pond is formed; The gas-tight silo that on this lathe bed cell wall, has been threaded lid, this Cang Gai or make for transparent organic glass, or make for stainless steel.
4, according to the experimental provision that the described simulation of claim 3 microorganism/big molecular film generates, it is characterized in that: described gas-tight silo lid, it also is equipped with the mechanism that carries out microexamination probe and scanning measurement system real-time photography, online.
5, according to the described experimental provision of simulating microorganism/big molecular film generation of claim 1, it is characterized in that: described circulation experiment liquid entry and exit, it is interconnected by conduit and peristaltic pump between this outer end, entry and exit except with the gas-tight silo two ends of flow cell bed communicate.
6, according to the experimental provision that the described simulation of claim 1 microorganism/big molecular film generates, it is characterized in that: described circulation experiment liquid, itself or contain bacterium culture medium solution for the aseptic seawater dilution of simulation microbiological corrosion environment; Itself or for including polysaccharide, the biological macromolecule solns of nucleic acid and protein; Itself or be the solution of the industrial condensate water environment of simulation, itself or be the solution of simulation sewage environment, itself or be simulation river, the solution of lake or briny environment.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200520124862 CN2844899Y (en) | 2005-11-18 | 2005-11-18 | Experiment device for generation of simulated microorgan/macromolecular membrane |
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| CN 200520124862 CN2844899Y (en) | 2005-11-18 | 2005-11-18 | Experiment device for generation of simulated microorgan/macromolecular membrane |
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| CN2844899Y true CN2844899Y (en) | 2006-12-06 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101226136B (en) * | 2008-01-30 | 2011-02-09 | 吉林大学 | Magnesium alloy microbial corrosion method based on solid medium |
| CN107132227A (en) * | 2017-05-11 | 2017-09-05 | 南开大学 | A kind of biomembrane film forming procedure online monitoring system and monitoring method |
| CN107561132A (en) * | 2017-08-24 | 2018-01-09 | 河海大学 | A kind of reaction unit for being used to study river course microbial film Factors affecting growth |
| CN110132905A (en) * | 2019-05-29 | 2019-08-16 | 清华大学 | Liquid pool device and its dynamic measurement system for Peace Park instrument |
| CN115078234A (en) * | 2022-05-06 | 2022-09-20 | 北京科技大学 | Microbial corrosion test device capable of simulating hydraulic conditions of gravity flow sewage pipeline |
| CN116042383A (en) * | 2023-01-06 | 2023-05-02 | 江苏海魄酒业股份有限公司 | Culture system and culture method for marine microorganisms for brewing wine |
-
2005
- 2005-11-18 CN CN 200520124862 patent/CN2844899Y/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101226136B (en) * | 2008-01-30 | 2011-02-09 | 吉林大学 | Magnesium alloy microbial corrosion method based on solid medium |
| CN107132227A (en) * | 2017-05-11 | 2017-09-05 | 南开大学 | A kind of biomembrane film forming procedure online monitoring system and monitoring method |
| CN107561132A (en) * | 2017-08-24 | 2018-01-09 | 河海大学 | A kind of reaction unit for being used to study river course microbial film Factors affecting growth |
| CN107561132B (en) * | 2017-08-24 | 2019-12-10 | 河海大学 | Reaction device for researching growth influence factors of riverway microbial film |
| CN110132905A (en) * | 2019-05-29 | 2019-08-16 | 清华大学 | Liquid pool device and its dynamic measurement system for Peace Park instrument |
| CN115078234A (en) * | 2022-05-06 | 2022-09-20 | 北京科技大学 | Microbial corrosion test device capable of simulating hydraulic conditions of gravity flow sewage pipeline |
| CN116042383A (en) * | 2023-01-06 | 2023-05-02 | 江苏海魄酒业股份有限公司 | Culture system and culture method for marine microorganisms for brewing wine |
| CN116042383B (en) * | 2023-01-06 | 2023-10-10 | 江苏海魄酒业股份有限公司 | Culture system and culture method for marine microorganisms for brewing wine |
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