CN2803310Y - Biological Reactor for artificial liver - Google Patents
Biological Reactor for artificial liver Download PDFInfo
- Publication number
- CN2803310Y CN2803310Y CN 200420114721 CN200420114721U CN2803310Y CN 2803310 Y CN2803310 Y CN 2803310Y CN 200420114721 CN200420114721 CN 200420114721 CN 200420114721 U CN200420114721 U CN 200420114721U CN 2803310 Y CN2803310 Y CN 2803310Y
- Authority
- CN
- China
- Prior art keywords
- woven fabrics
- bioreactor
- artificial liver
- winding
- woven cloth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000004185 liver Anatomy 0.000 title claims abstract description 19
- 238000004804 winding Methods 0.000 claims description 22
- 239000004745 nonwoven fabric Substances 0.000 claims description 21
- 235000012489 doughnuts Nutrition 0.000 claims description 15
- 230000002706 hydrostatic effect Effects 0.000 claims description 8
- 230000008676 import Effects 0.000 claims description 4
- 239000012510 hollow fiber Substances 0.000 abstract description 9
- 239000004744 fabric Substances 0.000 abstract description 7
- 230000008827 biological function Effects 0.000 abstract description 2
- 210000005229 liver cell Anatomy 0.000 abstract 3
- 208000031481 Pathologic Constriction Diseases 0.000 abstract 1
- 208000037804 stenosis Diseases 0.000 abstract 1
- 230000036262 stenosis Effects 0.000 abstract 1
- 210000003494 hepatocyte Anatomy 0.000 description 17
- 239000007788 liquid Substances 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 9
- 239000008280 blood Substances 0.000 description 9
- 239000000835 fiber Substances 0.000 description 8
- 210000002381 plasma Anatomy 0.000 description 7
- 230000001464 adherent effect Effects 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 206010019663 Hepatic failure Diseases 0.000 description 1
- 238000010824 Kaplan-Meier survival analysis Methods 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 208000007903 liver failure Diseases 0.000 description 1
- 231100000835 liver failure Toxicity 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a bioreactor for an artificial liver. On the basis of an existing bioreactor which uses hollow fiber inner cores, a plurality of hollow fiber inner cores are wrapped and rolled by non-woven cloth, thereby, the hollow fiber inner cores not only are attached to the non-woven cloth but also are kept with gaps. The non-woven cloth are wrapped and rolled so as to be arranged in a cylindrical container side by side. Hollow fiber wires are attached to the non-woven cloth so that convergence and stenosis are not generated easily. The non-woven cloth has good biocompatibility and has strong affinity for liver cells, thereby, liver cells can be easily attached to the non-woven cloth and carry out anchorage growth so as to exert the biological functions of liver cells.
Description
Technical field
This utility model belongs to field of biomedicine technology, relates to a kind of armarium, and a kind of is core apparatus---the bioreactor for artificial liver that biotype and hybrid artificial liver treatment are used.
Background technology
Bioreactor is the core apparatus of biotype and hybrid artificial liver support system, is the place that exogenous hepatocyte and blood samples of patients/blood plasma carry out mass exchange.Will meet following basic demand as bioreactor: middle small-molecule substance transmitted in both directions and macromolecular substances immunity intercept, ensure hepatocellular activity and a function and a constant volume.
Existing application is generally the hollow fiber reactor that cell culture is used in the bioreactor of artificial liver.So-called hollow fiber reactor is a branch of hollow fibre filament to be closed in the container (be generally cylindrical), and hollow fibre filament is divided into inside and outside two chambeies with container, is inner chamber in the hollow fibre filament, and silk is outer to be exocoel.Blood samples of patients and cultivation hepatocyte place two chambeies of hollow fibre filament respectively, and hollow fibre filament is made of special material, play the effect of immunity isolation and mass exchange.But general doughnut shape bioreactor defectiveness, because hepatocyte is the cell of adherent type, general adherent growth could be brought into play its physiological function effectively.But common hollow fiber reactor is unfavorable for managing the cultivation hepatocyte adherent growth of exocoel.Above-mentioned defective is the reason place that present bioartificial liver's treatment can not improve the liver failure curative effect.
Summary of the invention
The bioreactor for artificial liver that the design provided, comprise a hydrostatic column that a plurality of import and export are arranged, the doughnut cylinder core (6) that has a plurality of two ends of making to communicate in the container with doughnut, interior in-core is an inner chamber, inner core is outward an exocoel, be characterized in doughnut cylinder core (6) winding being become to contain the non-woven fabrics winding (5) that the two ends of doughnut cylinder core communicate, non-woven fabrics winding (5) is placed in the hydrostatic column side by side with non-woven fabrics.Doughnut cylinder core (6) in the non-woven fabrics winding (5) is loose shape attached on the non-woven fabrics, and the space between the inner core forms exocoel.
Described non-woven fabrics winding (5) can be a plurality of little windings, also can be that a big winding circle becomes concentric circles.
The inner chamber of hydrostatic column is imported and exported the two ends that (1,2) are arranged on hydrostatic column, and upper and lowerly respectively has exocoel to import and export (4,3).
Description of drawings
Fig. 1 is a bioreactor for artificial liver longitudinal section structural representation;
Fig. 2 is the cross section structural representation at point of contact with preflush outlet/sample tap 4 for bioreactor for artificial liver.
The specific embodiment
The profile of present embodiment bioreactor for artificial liver is a column type, transparent plastic shell.Size dimension is: long 10cm, the diameter of cross section is 20ml for the 2cm. volume.During actual fabrication, can or dwindle, formulate needed size according to the amplification of purposes needs.
As Fig. 1, shown in Figure 2: 1 is the blood/plasma inlet, enter the interior inner chamber of doughnut cylinder core for blood/plasma, 2 are the blood/plasma outlet, it is flow through flow export after the hollow fiber cavity of blood/plasma, 3 for entering the mouth towards liquid/hepatocyte in advance, inject hepatocyte suspension and pour by this inlet in advance towards liquid, 4 are preflush outlet/sample tap, during promptly pre-flushing and hepatocyte when injecting in advance towards the outlet of liquid, sample tap when also being simultaneously the artificial liver treatment, 5 of bioreactor inside is the non-woven fabrics winding, non-woven fabrics claims non-weaving cloth (nonwoven) again, winding shape supporter for hepatocellular attachment area and doughnut inner core, 6 is the doughnut cylinder core, is the path of blood/plasma, by the non-woven fabrics winding, and be attached to non-woven fabrics, 7 is cabinet.
The non-woven fabrics winding of this example is a plurality of little windings, and the diameter of doughnut inner core is about 1mm, and the diameter of non-woven fabrics winding is about 4mm, and the space of formation is for passing in and out towards liquid and hepatocyte suspension in advance.
The opening of doughnut inner core is positioned at columniform both sides, the sealing of the space between hollow fiber hereinto, and the two ends of non-woven fabrics winding are also closed.
The design compares following advantage with hollow type fiber reactor in the past: hollow fibre filament is attached on the non-woven fabrics, be difficult for to produce the mutual winding between the narrow and hollow fibre filament of inner chamber; Nonwoven is furnished with good biocompatibility, and hepatocyte is had very strong affinity, and hepatocyte is easy to be attached on the non-woven fabrics and adherent growth is brought into play its biological function.
In advance towards liquid/hepatocyte inlet be positioned on the cylinder of cylinder blanket towards liquid outlet/sample tap in advance, in advance towards liquid/hepatocyte inlet and in advance towards liquid outlet/sample tap opening that direction is set is opposite, when dashing with the hepatocyte injection in advance, just can at utmost reduce exocoel the volume of dead space like this, help enlarging effective exchange area, increase dischargeable capacity.
The use of the design's bioreactor for artificial liver is below described with the example of model pig.
Use the exocoel and the inner chamber of the normal saline flushing bioreactor that contains heparin earlier, separate in aseptic condition and to obtain former generation porcine hepatocyte, hepatocyte suspension is injected the exocoel of bioreactors by pre-towards liquid/hepatocyte inlet 3, the preflush body of exocoel seals 3 and 4 after hepatocyte suspension substitutes preflush fully from discharging towards liquid outlet/sample tap 4 in advance simultaneously.Subsequently the blood of model pig is introduced from blood/plasma inlet 1, from 2 discharges, failed back again in the body of model pig behind the bioreactor of flowing through then.Keep blood circulation and finish treatment after a few hours.The Kaplan-Meier survival analysis shows the time-to-live that this treatment can the significant prolongation animal subject.
The design's bioreactor of artificial liver is disposable medical apparatus and instruments.
Claims (4)
1, a kind of bioreactor for artificial liver, comprise a hydrostatic column that a plurality of import and export are arranged, the doughnut cylinder core (6) that has a plurality of two ends of making to communicate in the container with doughnut, interior in-core is an inner chamber, inner core is outward an exocoel, it is characterized in that: with non-woven fabrics doughnut cylinder core (6) winding is become to contain the non-woven fabrics winding (5) that the two ends of doughnut cylinder core communicate, non-woven fabrics winding (5) is placed in the hydrostatic column side by side.
2, by the described bioreactor for artificial liver of claim 1, it is characterized in that: the doughnut cylinder core (6) in the described non-woven fabrics winding (5) is loose shape attached on the non-woven fabrics, and the space between the inner core forms exocoel.
3, by claim 1 or 2 described bioreactor for artificial liver, it is characterized in that: described non-woven fabrics winding (5) can be a plurality of little windings, also can be that a big winding circle becomes concentric circles.
4, by the described bioreactor for artificial liver of claim 1, it is characterized in that: the inner chamber of described hydrostatic column is imported and exported the two ends that (1,2) are arranged on hydrostatic column, and upper and lowerly respectively has exocoel to import and export (4,3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420114721 CN2803310Y (en) | 2004-12-24 | 2004-12-24 | Biological Reactor for artificial liver |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420114721 CN2803310Y (en) | 2004-12-24 | 2004-12-24 | Biological Reactor for artificial liver |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2803310Y true CN2803310Y (en) | 2006-08-09 |
Family
ID=36908614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200420114721 Expired - Fee Related CN2803310Y (en) | 2004-12-24 | 2004-12-24 | Biological Reactor for artificial liver |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2803310Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101555453B (en) * | 2008-06-04 | 2012-07-04 | 复旦大学附属华山医院 | Endothelial cell bioreactor as well as preparation method and application thereof |
| CN106267397A (en) * | 2016-07-29 | 2017-01-04 | 武汉仝干医疗科技股份有限公司 | Lint wire type bioreactor bioartificial liver system |
-
2004
- 2004-12-24 CN CN 200420114721 patent/CN2803310Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101555453B (en) * | 2008-06-04 | 2012-07-04 | 复旦大学附属华山医院 | Endothelial cell bioreactor as well as preparation method and application thereof |
| CN106267397A (en) * | 2016-07-29 | 2017-01-04 | 武汉仝干医疗科技股份有限公司 | Lint wire type bioreactor bioartificial liver system |
| CN106267397B (en) * | 2016-07-29 | 2019-02-05 | 武汉仝干医疗科技股份有限公司 | Lint wire type bioreactor bioartificial liver system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| C57 | Notification of unclear or unknown address | ||
| DD01 | Delivery of document by public notice |
Addressee: Bio-system Medical Technology (Shanghai) Co., Ltd. Document name: Decision of the request for invalidation |
|
| C32 | Full or partial invalidation of patent right | ||
| IP01 | Partial invalidation of patent right |
Decision date of declaring invalidation: 20080624 Decision number of declaring invalidation: 11786 |