CN2736759Y - Micro flow controlled chip laser induction fluorescent analyzer - Google Patents
Micro flow controlled chip laser induction fluorescent analyzer Download PDFInfo
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- CN2736759Y CN2736759Y CN 200420069911 CN200420069911U CN2736759Y CN 2736759 Y CN2736759 Y CN 2736759Y CN 200420069911 CN200420069911 CN 200420069911 CN 200420069911 U CN200420069911 U CN 200420069911U CN 2736759 Y CN2736759 Y CN 2736759Y
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- optical filtering
- color filter
- laser
- transparent semi
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model provides a micro flow controlled chip laser induction fluorescent analyzer. The utility model is composed of an illuminating source (1), a color filter I (2), an object lens (4), a semi-transparent semi-reflection mirror (5), a color filter II (6), a pin hole (8) and a photomultiplier tube (9). The illuminating source and the color filter are sequentially arranged on the cross light axial line of the semi-transparent semi-reflection mirror. The object lens is arranged on the upper part of the lengthwise light axial line of the semi-transparent semi-reflection mirror. The color filter, the collecting mirror, the pin hole and the photomultiplier tube are sequentially arranged at the bottom of the lengthwise light axial line of the semi-transparent semi-reflection mirror. The data acquisition AD transmission of the photomultiplier tube is communicated with the computer operating platform. The illuminating source is a laser, and the wave length of the laser is 520nm-640nm. The color filter I and the color filter II are all the changeable color filters, and the wave length of the changeable color filter is 520nm-640nm. The utility model is characterized in that: the selection range of the detecting used dye is widened; the direct data is provided, and the use is convenient and reliable.
Description
Technical field:
The utility model relates to a kind of optical instrument in the analytical chemistry field, computer-controlled optical instrument.
Background technology:
By the micro-fluidic chip analyzer that Canadian Albert company produces, its ultimate principle is that the 530nm laser that utilizes semiconductor laser to produce becomes the comparison monochrome through an optical filter light shines in the pipeline of micro-fluidic chip through object lens then through a semi-transparent semi-reflecting lens; If there is fluorescent material to exist in the pipeline, its () can be emitted the fluorescence of certain wavelength after obtaining energy under the irradiation of laser, then, this fluorescence enters optical circuit through object lens, filters out through the light of an optical filter with non-wavelength of fluorescence again, fluorescence enters on the photomultiplier through pin hole again, through amplifying, light signal is transformed into electric signal, again through signal gathering unit arrangement input computing machine, through software processes, on computer screen, show its time-voltage curve.Its instrument formation comprises three parts: computing machine, circuit and high voltage control module, light path and operating platform.Each several part is member alone, connects by lead.Its weak point:
1. be focused to manually, extremely inconvenient, and to seek out the better repeatability difficulty;
2. emission wavelength has only wavelength of 570 nanometers, has limited the selection of dyestuff like this, and bigger limitation is arranged;
3. software no current-time curve detects and shows, then analyzes difficulty as breaking down when causing operation;
4. this kind instrument does not have the direct result of calculation of data such as curve peak area, peak height, half-peak breadth;
2100 its principles of type biological analyser of being produced by Anji human relations company are basic identical with aforesaid machine principle, are its optical maser wavelength difference, and the latter is 630 nanometers, cause emission wavelength also different.Its apparatus structure divides two parts: computer control part and operating platform and the integrated part of optical detection.
Operating platform is made up of 16 fixing electrodes.Its weak point:
1. adopt manufacturer's special chip.Because electrode is fixing size dimension, so, special chip can only be used, so the chip of not opening to the outside world uses;
2. instrument is strong, limited flexibility system;
3. excitation wavelength is 630 nanometers, and the transmitted wave wavelength is single, does not have and selects;
4. not having time current curve shows.
Summary of the invention:
The micro-fluidic chip laser-induced fluorescence analyzer that provides a kind of usable range wider is provided the purpose of this utility model, and has the CCD monitoring, and computer screen shows and focus on that content displayed is more.
The utility model provides a kind of micro-fluidic chip laser-induced fluorescence analyzer, is made of light source (1), optical filtering I (2), object lens (4), semi-transparent semi-reflecting lens (5), optical filtering II (6), condenser (7), pin hole (8), photomultiplier (9); Light source (1) and optical filtering I (2) place on the horizontal smooth axistyle of semi-transparent semi-reflecting lens (5) successively, object lens (4) place on semi-transparent semi-reflecting lens (5) vertical light axistyle up, optical filtering II (6), condenser (7), pin hole (8) and photomultiplier (9) place successively on semi-transparent semi-reflecting lens (5) vertical light axistyle below; The data acquisition A D conversion of photomultiplier (9) communicates to connect through high-voltage relay control circuit, high pressure output control circuit and high pressure output detection circuit and computing machine, operating platform; Light source (1) is that wavelength is the laser instrument of 520nm～640nm, and optical filtering I (2) and optical filtering II (6) are that wavelength is the interchangeable optical filtering of 520nm～640nm, to reach the optical filtering accuracy and to enlarge range of application.
The pin hole (8) of the micro-fluidic chip laser-induced fluorescence analyzer that provides at the utility model locates to be provided with ccd video camera (10), and the information that ccd video camera (10) obtains is delivered to computing machine, to detect.
The light source (1) of the micro-fluidic chip laser-induced fluorescence analyzer that the utility model provides, optical filtering I (2) and optical filtering II's (6) all is that wavelength is the device of 532nm.
The micro-fluidic chip laser-induced fluorescence analyzer that the utility model provides can use software output spectrogram, and spectrogram comprises omnidistance four figure of image that detect of voltage-time curve, electric current-time curve, PMT-time curve and some check point CCD.
The operation system of software of the micro-fluidic chip fluorescence analyser that the utility model provides can be made up of three parts:
1. separating controlling program comprises that selection of time and voltage-regulation that CCD detects light path click focusing down and detachment process control;
2. data collection section, by four output voltage-time curves of screen display, electric current-time curve, the omnidistance image that detects of PMT-time curve and/or some check point CCD is formed;
3. data processing section.
The characteristics of the micro-fluidic chip fluorescence analyser that the utility model provides are as follows:
1. optical filter is replaceable, has widened the range of choice that detects with dyestuff greatly;
2. adopt the CCD monitoring, computer screen shows focusing, and is easy to use and reliable;
3. except that demonstration time-voltage curve, also show time-current curve simultaneously; Time-current curve is very favourable to the situations such as obstruction that monitoring takes place in the split tunnel, and the calculating to electroosmotic flow simultaneously provides direct data, and data computation becomes simply, fast so for this reason.
Description of drawings:
Fig. 1 is a structural representation of the present utility model;
Fig. 2 is a principle index path of the present utility model;
Fig. 3 is the communication link map interlinking of computer software of the present utility model.
Embodiment:
The formation of this micro-fluidic chip fluorescence analyser comprises light path, is used for the module and the operating platform of circuit and high voltage control, implements its every function by computer software.
The structural representation of this instrument is seen Fig. 1.
The principle index path of this instrument is seen Fig. 2.
Fig. 3 is seen in the communication link map interlinking of the computer software of this instrument.
Utilize 532nm laser that semiconductor laser produces after filtration light microscopic I (2) light that becomes the comparison monochrome shine in the pipeline of micro-fluidic chip (3) through object lens (4) then through a semi-transparent semi-reflecting lens (5); If there is fluorescent material to exist in the pipeline, its () can be emitted the fluorescence of certain wavelength after obtaining energy under the irradiation of laser, then, this fluorescence enters optical circuit through object lens (4), through optical filtering II (6) light of non-wavelength of fluorescence is filtered out, fluorescence enters on the photomultiplier (9) through condenser (7) and pin hole (8) more again, through amplifying, light signal is transformed into electric signal, again through photomultiplier (9) data acquisition A D conversion portion input computing machine.
This instrument adopts the CCD monitoring, and computer screen shows focusing; Optical filter is replaceable, to have widened the range of choice of experiment with dyestuff.
This instrument comprises omnidistance four in the image that detects of voltage-time curve, electric current-time curve, PMT-time curve and some check point CCD with known software approach output spectrogram, spectrogram.
This instrument software operating system is made up of three parts:
1. separating controlling program comprises that selection of time and voltage-regulation that CCD detects light path click focusing down and detachment process control;
2. data collection section, by the output voltage-time curve of screen display, electric current-time curve, the omnidistance image that detects of PMT-time curve and some check point CCD is formed;
3. data processing section.Carry out rhodamine (Rhodamine) 6G and DNA sample analysis with the PMMA chip on this instrument, its detection sensitivity reaches 6.67 * 10 when detecting rhodamine 6G
-13Mol/L.
Claims (3)
1, micro-fluidic chip laser-induced fluorescence analyzer is characterized in that: be made of light source (1), optical filtering I (2), object lens (4), semi-transparent semi-reflecting lens (5), optical filtering II (6), condenser (7), pin hole (8), photomultiplier (9); Light source (1) and optical filtering I (2) place on the horizontal smooth axistyle of semi-transparent semi-reflecting lens (5) successively, object lens (4) place on semi-transparent semi-reflecting lens (5) vertical light axistyle up, optical filtering II (6), condenser (7), pin hole (8) and photomultiplier (9) place successively on semi-transparent semi-reflecting lens (5) vertical light axistyle below; The AD conversion of photomultiplier (9) is gathered, and communicates to connect with computing machine and operating platform; Light source (1) is that wavelength is the laser instrument of 520nm～640nm, and optical filtering I (2) and optical filtering II (6) are that wavelength is the interchangeable optical filtering of 520nm～640nm.
2, according to the described micro-fluidic chip laser-induced fluorescence analyzer of claim 1, it is characterized in that: locate to be provided with ccd video camera (10) at described pin hole (8).
3, according to claim 1 or 2 described micro-fluidic chip laser-induced fluorescence analyzers, it is characterized in that: described light source (1), optical filtering I (2) and optical filtering II (6) are that wavelength is the device of 532nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420069911 CN2736759Y (en) | 2004-07-22 | 2004-07-22 | Micro flow controlled chip laser induction fluorescent analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420069911 CN2736759Y (en) | 2004-07-22 | 2004-07-22 | Micro flow controlled chip laser induction fluorescent analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2736759Y true CN2736759Y (en) | 2005-10-26 |
Family
ID=35307494
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200420069911 Expired - Fee Related CN2736759Y (en) | 2004-07-22 | 2004-07-22 | Micro flow controlled chip laser induction fluorescent analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2736759Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770078A (en) * | 2016-11-14 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of LIF food detector |
-
2004
- 2004-07-22 CN CN 200420069911 patent/CN2736759Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770078A (en) * | 2016-11-14 | 2017-05-31 | 无锡艾科瑞思产品设计与研究有限公司 | A kind of LIF food detector |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |