CN2705787Y - Reader for suspending biological chip - Google Patents
Reader for suspending biological chip Download PDFInfo
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- CN2705787Y CN2705787Y CN 200420045344 CN200420045344U CN2705787Y CN 2705787 Y CN2705787 Y CN 2705787Y CN 200420045344 CN200420045344 CN 200420045344 CN 200420045344 U CN200420045344 U CN 200420045344U CN 2705787 Y CN2705787 Y CN 2705787Y
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- reading apparatus
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- biochip
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Abstract
A kind of reading apparatus of suspension biochip comprises the diluting tank that is used to dilute described biochip; Produce low pressure and be used for the waste liquid bottle of splendid attire waste liquid; Be used to produce the excitation source of high light; Be used to connect the conduit of described diluting tank 1 and waste liquid bottle and the detection case that is used to support described conduit.When reading, only can pick out the kind of probe or antigen, antibody by the size of surveying microballoon, with respect to the method for the marker color of surveying microballoon, not only simple and reliable, and the requirement of equipment greatly lowered, thereby also lowered processing and detected cost.
Description
Technical field
The utility model relates to the biological technical field of a new generation, relates in particular to a kind of reading instrument of novel suspension biochip.
Background technology
Biochip is used for the detection of biological sample, this technology is born in the beginning of the eighties in last century, be integrated with thousands of the informational molecules relevant with life by micro fabrication in chip, it can carry out integrated to the various biochemical reaction process in life science and the medical science.Be compared to traditional biochemistry detection technology, its advantage is can test simultaneously in theory and analyze several even several thousand, several ten thousand kinds of different Biological indicators.The gene diagnosis and the fields such as antigen, antibody test that are widely used in gene sequencing, detection in Gene Mutation and polymorphism analysis, genomic library pattern analysis, disease.Be considered to new century one of the most promising biological technical field.
Biochip is divided into microarray formula and floated (claiming streaming again) two kinds by the carrier difference.Microarray formula biochip, briefly on the silicon chip that poly-D-lysine bag quilt is arranged of a thumbnail, probe biomolecule is arranged with the form of large scale array exactly, formation can interact with molecules of interest, the solid phase surface of Bank of Communications's reaction, exciting down mark fluorescent to present different fluorescence emission spectrums respectively according to the real reaction situation in the order of laser levies, CCD camera or laser confocal microscope are according to its wavelength and wave amplitude feature collection signal, Computer Analysis data result.What the most successful isocyanides formed certain commercial market at present is the DNA chip, is about to countless pre-designed oligonucleotides or cDNA and makes dot matrix on chip, with homologous nucleic acid molecular hyridization [3] in the sample.Allow the biomolecule of almost all kinds to make probe on the chip in fact in theory, such as albumen, sugar, lipid and other micromolecule.Can measure simultaneously in theory and reach several ten thousand indexs, but the preparation more complicated of chip, the preparation cost height, the labeling process of sample is loaded down with trivial details, and signal sensitivity is low, the poor repeatability of test.Make this technology can only be used for scientific research, be difficult in clinical diagnosis, popularize.
The suspension biochip technology is called streaming technology again, is the significant improvement to microarray technology.In the prior art, its technical characterstic is: use the carrier of the microballoon of different colours as different bioprobes, the color of microballoon and the kind of bioprobe are corresponding one by one, microballoon is done the Two Colour Fluorescence mark of varying strength, make microballoon line up a line on the track of determining by liquid flow field during detection, order is passed through detecting area.Two restraint the laser cross-fires at detecting area, a branch of marker color that is used for surveying microballoon, and another bundle is used for measuring the fluorescence intensity of composition to be measured.This technology is compared with microarray formula technology, and advantage is to be easy to produce in enormous quantities, and measuring repeatability is good, and is highly sensitive.Shortcoming is that microballoon preparation is complicated, and reaction system is complicated, and background will be through repeatedly washing, and operation is difficult for, and simultaneously, is used to survey the equipment requirements height of the color of microballoon.
Summary of the invention
In order to overcome the deficiency of existing biochip and reading technique, the purpose of this utility model is: the reading instrument that a kind of biochip is provided, can detect the kind of different bioprobes easily, and judge whether contain certain composition to be measured and concentration thereof in the sample.
The technical scheme that its technical matters that solves the utility model adopts is: a kind of reading apparatus of suspension biochip is characterized in that: described reading apparatus comprises the diluting tank that is used to dilute described biochip; Produce low pressure and be used for the waste liquid bottle of splendid attire waste liquid; Be used to produce the excitation source of high light; Be used to connect the conduit of described diluting tank 1 and waste liquid bottle and the detection case that is used to support described conduit.
The beneficial effects of the utility model are, suspension biochip since the microballoon that adopts different sizes as the carrier of different bioprobes or antigen, antibody, the kind of the size of microballoon and probe or antigen, antibody is corresponding one by one, make reading apparatus when reading, only can pick out the kind of probe or antigen, antibody by the size of surveying microballoon, method with respect to the marker color of surveying microballoon, not only simple and reliable, and the requirement of equipment greatly lowered, thereby also lowered processing and detected cost.Method by abundant diluted mixture liquid has replaced the method for background through repeatedly washing, and makes simple to operateization equally, has improved detection efficiency simultaneously.Therefore, can be widely used in the blood station to blood test, hospital makes a definite diagnosis patient and other various biological technical fields.Use the analyzing biochips human genome, can find out cancer, diabetes and cause the gene that cause a disease of disease, will greatly improve the health of the people and the general level of the health by genetic defective.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further specified.
Fig. 1 is the structural representation of the utility model suspension biochip reading apparatus.
The explanation of reference numeral:
The 1-diluting tank; The 2-electrode; The 3-photomultiplier; The 4-condenser; The 5-excitation source; The 6-waste liquid bottle; The 7-electrode; 8-microballoon to be measured; The 9-detection case; The 10-aperture; The 11-paddle.
Embodiment
As shown in Figure 1, the utility model suspension biochip reading apparatus includes but not limited to structure: the diluting tank 1 that is used to dilute described biochip; Produce low pressure and be used for the waste liquid bottle 6 of splendid attire waste liquid; Be used to produce the excitation source 5 of high light; Be used to connect the conduit 12 of described diluting tank 1 and waste liquid bottle 6; Be used to support the detection case 9 of described conduit 12.
Be placed with paddle 11 and electrode 2 in the described diluting tank 1, the bottom of the logical diluting tank 1 of conduit 12 1 terminations is convenient to liquid and is flowed out, and the other end connects the top of waste liquid bottle 6, is convenient to liquid and flows into waste liquid bottle 6.Described conduit 12 stage casings are provided with aperture 10, the aperture of aperture 10 is greater than the maximum gauge of microballoon, preferably scheme is, the aperture of aperture 10 is about 3: 2 with the ratio of the maximum gauge of microballoon, when flow of liquid during through aperture 10, the microballoon that floats on a liquid can only be to line up a line on the track of determining, order can accurately be carried out thereby be convenient to detect by detecting area.Described conduit 12 back segments are provided with electrode 7, because the electric action of liquid forms path between the electrode 2 and 7.Excitation source 5 is positioned at the upside of detection case 9, and the high light that is sent by excitation source 5 passes through condenser 4, focuses in the conduit 12, forms detecting area.Microballoon 8 to be measured reflexes to light on the photomultiplier 3.
During detection, pour this chip into reaction utensil, add testing sample and fluorescent marker again, react certain hour at a certain temperature.If contain certain composition to be measured in the sample, then can form microballoon---composition to be measured---complex of label.The principle of work of pick-up unit (reading apparatus) is as follows: the size of measuring microballoon can adopt aperture resistance principle (claiming " Coulter principle " again), photoresistance principle or light scattering (diffraction) principle.
Be that example explains with aperture resistance principle below, as shown in Figure 5.Reacted mixed liquor is moved into diluting tank 1, rush in the electrolytic solution of capacity again in the pond, mixed liquor is fully diluted, the concentration of fluorescent marker is just enough low freely in the unit volume dilution like this.Allow paddle 11 keep rotating, make microballoon keep suspended state.Allow waste liquid bottle 6 be in the infrabar state, promptly be lower than atmospheric pressure, then microballoon will flow to waste liquid bottle along with electrolytic solution.When microballoon during through small holes 10, owing to blocked the partially conductive cross section, the resistance between two electrodes 2 and 7 will increase.Electric-resistivity method granulometry principle is told us, and the resistance rise and peak is proportional to the volume of particle.Therefore can judge the kind of the microballoon that flows through aperture by the variation of measuring resistance.When microballoon continued to pass through the fluorescence measurement district forward, microballoon was by the strong illumination from excitation source 5, thus the generation fluorescent radiation.Fluorescence is accepted by photomultiplier 3.Can judge whether contain certain in the sample by fluorescence intensity with composition to be measured and concentration thereof.
Because the quantity of every kind of microballoon has hundreds and thousands of, so the measured value of each project all is the mean values of measuring for hundreds and thousands of times, and the reliability of measurement result is very high.Secondly, every kind of bead all can be mass-produced, and wraps respectively and is mixed behind last a certain probe or antigen, the antibody again, therefore be easy to produce in enormous quantities, and with low cost.The 3rd, after chip and sample and the label reaction, need not wash background, only need dilution just can measure, greatly facilitate use.
Claims (8)
1, a kind of reading apparatus of suspension biochip is characterized in that: described reading apparatus comprises the diluting tank that is used to dilute described biochip; Produce low pressure and be used for the waste liquid bottle of splendid attire waste liquid; Be used to produce the excitation source of high light; Be used to connect the conduit of described diluting tank 1 and waste liquid bottle and the detection case that is used to support described conduit.
2, the reading apparatus of suspension biochip according to claim 1 is characterized in that: be placed with paddle in the described diluting tank.
3, the reading apparatus of suspension biochip according to claim 1 and 2 is characterized in that: be placed with electrode in the described diluting tank, described conduit back segment is provided with electrode equally, and described liquid has electric conductivity, forms path between two electrodes.
4, the reading apparatus of suspension biochip according to claim 3 is characterized in that: the bottom of the logical diluting tank of conduit one termination, and the other end connects the top of waste liquid bottle, and liquid flows into waste liquid bottle from diluting tank by described conduit.
5, the reading apparatus of suspension biochip according to claim 3 is characterized in that: described conduit stage casing is provided with aperture, and the aperture of aperture is about 3: 2 with the ratio of the maximum gauge of microballoon.
6, according to the reading apparatus of claim 4 or 5 described suspension biochips, it is characterized in that: excitation source is positioned at the upside of detection case, and the high light that is sent by excitation source passes through condenser, focuses in the conduit.
7, the reading apparatus of suspension biochip according to claim 3 is characterized in that: described paddle keeps rotating, and makes microballoon keep suspended state.
8, the reading apparatus of suspension biochip according to claim 3 is characterized in that: described waste liquid bottle is in the infrabar state, promptly is lower than atmospheric pressure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420045344 CN2705787Y (en) | 2004-04-29 | 2004-04-29 | Reader for suspending biological chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200420045344 CN2705787Y (en) | 2004-04-29 | 2004-04-29 | Reader for suspending biological chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2705787Y true CN2705787Y (en) | 2005-06-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200420045344 Expired - Fee Related CN2705787Y (en) | 2004-04-29 | 2004-04-29 | Reader for suspending biological chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2705787Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424698A (en) * | 2015-11-25 | 2016-03-23 | 江苏三联生物工程有限公司 | Biochip reading instrument with gripper function |
| CN106645067A (en) * | 2016-12-15 | 2017-05-10 | 天津岳达科技有限公司 | Liquid biochip analyzer |
-
2004
- 2004-04-29 CN CN 200420045344 patent/CN2705787Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424698A (en) * | 2015-11-25 | 2016-03-23 | 江苏三联生物工程有限公司 | Biochip reading instrument with gripper function |
| CN105424698B (en) * | 2015-11-25 | 2018-05-15 | 江苏三联生物工程有限公司 | A kind of biological chip reading apparatus with handgrip function |
| CN106645067A (en) * | 2016-12-15 | 2017-05-10 | 天津岳达科技有限公司 | Liquid biochip analyzer |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |