CN2693770Y - Assembling magnetic separation bracket with base - Google Patents

Assembling magnetic separation bracket with base Download PDF

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Publication number
CN2693770Y
CN2693770Y CNU032316925U CN03231692U CN2693770Y CN 2693770 Y CN2693770 Y CN 2693770Y CN U032316925 U CNU032316925 U CN U032316925U CN 03231692 U CN03231692 U CN 03231692U CN 2693770 Y CN2693770 Y CN 2693770Y
Authority
CN
China
Prior art keywords
pedestal
magnetic separator
separator frame
base
pilot hole
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CNU032316925U
Other languages
Chinese (zh)
Inventor
王刚
杨百全
庄家骐
江林
杨文胜
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Dongcheng Nano Science & Techno ogy Development Co ltd
Original Assignee
Shanghai Dongcheng Nano Science & Techno ogy Development Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Dongcheng Nano Science & Techno ogy Development Co ltd filed Critical Shanghai Dongcheng Nano Science & Techno ogy Development Co ltd
Priority to CNU032316925U priority Critical patent/CN2693770Y/en
Application granted granted Critical
Publication of CN2693770Y publication Critical patent/CN2693770Y/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/025—High gradient magnetic separators
    • B03C1/031—Component parts; Auxiliary operations
    • B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
    • B03C1/0332—Component parts; Auxiliary operations characterised by the magnetic circuit using permanent magnets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/30—Combinations with other devices, not otherwise provided for
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)

Abstract

The utility model discloses a magnetic separation bracket which contains at least a magnetic bracket; the magnetic bracket contains a cylinder part whose inner is provided with a magnet, a first base arranged at one end of the cylinder part, and a second base arranged at the other end of the cylinder part. Wherein the first base is provided with at least one first position hole, the second base is provided with at least one second position hole whose dimension is different from the first position hole. The utility model also contains a socket, and a slideway which can be matched with one or two of the first base and the second base of the magnetic bracket is arranged at the socket. The utility model is convenient to be used, and suitable for various centrifuge tubes.

Description

The magnetic the assembled separator frame of tool base
Technical field
The utility model relates to the DNA separation technology field, or rather, relates to a kind of magnetic separator frame.This magnetic separator frame is a kind of magnetic separating apparatus, is mainly used in enrichment magneticsubstance from solution, thereby realizes separating of magneticsubstance and liquid, is specially adapted to the separation of biomaterial.
Background technology
The application of magnetic separation technique in the separation of materials such as medicine, biomolecules more and more widely.Compare convenient and swift, characteristics such as required equipment is simple, extraction efficiency height that magnetic separation technique has with separation method such as the precipitator method, centrifuging, ion exchange method and various chromatography methods commonly used.
The isolating roughly process of magnetic is, can join in conjunction with the magneticsubstance of sample to be separated in the solution that contains sample to be separated, etc. the magnetic material with after sample to be separated fully combines, by magnetic separating apparatus solution is applied a foreign field, the magneticsubstance enrichment from solution that is combined with sample to be separated under the effect of outside magnetic field is come out, thereby realizes the separation of sample to be separated.
The magnetic separator frame is a kind of magnetic separating apparatus simple in structure, easy to use.The centrifuge tube that sample will be housed during use is put into the magnet stand hole, and the magneticsubstance in the sample can be enriched in the tube wall near magnetic separator frame pillar one side, with pipettor liquid is siphoned away then and can realize the separation of magneticsubstance from solution.
Yet existing magnetic separator frame product in use also exists some problems:
(1) the magnetic separator frame of general a kind of model can only with the supporting use of a kind of centrifuge tube of volume, so in use just restricted, and will be equipped with different magnetic separator frames at different sample volume to the volume of sample.
(2) magneticsubstance also has certain enrichment at the pipe end, thereby magneticsubstance also will be siphoned away the loss that causes material to be separated like this when imbibition except the tube wall enrichment in the sepn process.
(3) the magnetic separator frame is generally double-pore structure, when carrying out the separation of a plurality of samples, will use a plurality of magnet stands simultaneously, and each magnetic separator frame is discrete, and this inconvenience that can cause use is such as obscuring of sample takes place easily.
Therefore, this area presses for the magnetic separator frame of new easy to use, the suitable multiple centrifuge tube of exploitation.
Summary of the invention
The purpose of this utility model is at the problems referred to above, and a kind of magnetic separator frame of easy to use, suitable multiple centrifuge tube is provided.
The utility model provides a kind of assemblnig magnetic separator frame, and it comprises following assembly:
At least one magnet stand, described magnet stand comprises: one is provided with the cylinder part of magnet, second pedestal that first pedestal and that is arranged on described cylinder part one end is arranged on the described cylinder part the other end in it, wherein, described first pedestal is provided with at least one first pilot hole, and described second pedestal is provided with second pilot hole that at least one size is different from described first pilot hole;
One base, its be provided with can with first pedestal of described magnet stand and or two slide rails that match in second pedestal.
In another preference, the diameter of first pilot hole is 7.5 ± 1 millimeters.
In another preference, the diameter of second pilot hole is 10 ± 2 millimeters.
In another preference, the number of described magnet stand is 2-100, and more preferably the number of described magnet stand is 4-25.
In another preference, the pilot hole number on first pedestal and second pedestal is 1-5, more preferably is 2-4.
In another preference, described pilot hole be shaped as circle.
In another preference, the slide rail on the described base all cooperates with first pedestal and second pedestal of described magnet stand.
In another preference, each pilot hole is provided with the V-arrangement baffle plate.
Description of drawings
Fig. 1 is the side-view of magnet stand assembly in the magnetic separator frame of the present utility model.
Fig. 2 a and 2b are the side-looking photos of the utility model magnet stand and overlook photo.
Fig. 3 is the photo of base assembly in the magnetic separator frame of the present utility model.
Fig. 4 is the photo of magnetic separator frame of the present utility model.
Photo of the present utility model when Fig. 5 is to use the 1.5ml centrifuge tube.
Photo of the present utility model when Fig. 6 is to use the 0.5ml centrifuge tube.
Embodiment
Improve the structure of magnetic separator frame by extensive studies through going deep into for the utility model people.Magnetic separator frame after the improvement can be applicable to the centrifuge tube of at least two kinds of sizes simultaneously, can control magneticsubstance fully in the enrichment of tube wall place, has farthest reduced the loss of material to be separated; Can assemble and be formed for a plurality of sample separation ground combined magnetic separator frame.
Magnetic separator frame of the present utility model is made up of base and magnet stand two parts.
Referring to Fig. 1, Fig. 2 a and Fig. 2 b.Shown a kind of magnet stand 10 of the present utility model among the figure.Magnet stand comprises:
First pedestal 14 and one that the one cylinder part 12, that is provided with magnet in it is arranged on described cylinder part one end is arranged on second pedestal 16 of the described cylinder part the other end, wherein, described first pedestal 14 is provided with at least one first pilot hole 24, and described second pedestal 16 is provided with second pilot hole 26 that at least one size is different from described first pilot hole 24.In pilot hole, can place centrifuge tube 50.
In a preference, magnet stand 10 is divided into 2 parts, and the shape of every part is a square body in the middle of all being.One end of square body is a notch, can connect chimeric 2 parts, and chimeric position is a hollow.
Be not particularly limited at cylinder part 12 built-in magnet, be preferably 2000～3500 Gausses' magnet.
In another preference, 2 pilot holes are arranged on the base plane, be hollow recess between the pilot hole, be used for placing labels.Pilot hole is positioned at two ends, the hollow recess left and right sides, and 2 V-arrangement baffle plates are arranged in the hole, plays the fixed container effect.Can place the containers such as centrifuge tube of different size in the hole.
Referring to Fig. 3.Shown a kind of base of the present utility model among the figure.Base is a rectangle, and parallel relative notch is arranged at the top, can chimeric a plurality of magnet stands.
In another preference, pedestal (14,16) and cylinder part (12) is vertical mutually, and the planar of pedestal is big or small conforms to the notch size of base, can embed base.A kind of magnetic separator frame of the formation that magnet stand and base assemble as shown in Figure 4.
During use, after the centrifuge tube that magnetic nano-particle and sample are housed was positioned over positioning hole corresponding, the magnetic particle in 1 minute in the pipe will be adsorbed on the one side near the magnet stand center pillar, will abandon liquid then and inhale and go, repeatedly wash with washings again, repeat above magnetic and put forward operation steps.Fig. 5 and Fig. 6 have shown the situation of using 1.5ml and 0.5ml centrifuge tube respectively.
The utility model is particularly suitable for extracting DNA with magnetic nanometer composite material.In addition, can also be used for other biomolecules such as protein separates.
Major advantage of the present utility model is:
(a) be simple and easy to the characteristics of usefulness.This magnetic separator frame is applicable to the centrifuge tube of two kinds of volumes simultaneously.
Can control magnetic substance when (b) using and be enriched in tube wall fully.
(c) thus a plurality of magnetic separator frame can combine and is used for the separation of a plurality of samples.
Below in conjunction with specific embodiment, further set forth the utility model.Should be understood that these embodiment only to be used to the utility model is described and be not used in the restriction scope of the present utility model.The experimental technique of unreceipted actual conditions in the following example is usually according to the normal condition or the condition of advising according to manufacturer.
Embodiment 1
Use magnet stand shown in Fig. 2 a and the 2b and the magnetic separator frame that base constituted shown in Figure 3 to adsorb magnetic substance in the present embodiment.
Shown in Fig. 2 a and 2b, the magnet stand 10 of magnetic separator frame comprises two pedestals (14 and 16), and the pilot hole (24 and 26) in two kinds of apertures is arranged respectively on the pedestal, and these two kinds of pedestals can be respectively applied for the centrifuge tube of placing two kinds of different sizes.According to sample volume commonly used in the bioseparation, the magnetic separator frame can with the supporting use of centrifuge tube of 1.5ml and two kinds of volumes of 0.5ml, wherein pedestal 14 is used to place the 1.5ml centrifuge tube, pedestal 16 is used to place the 0.5ml centrifuge tube.
Marked the position, magnetic field of magnetic separator frame among Fig. 2 a, when the centrifuge tube that sample is housed placed on the magnetic separator frame, the strength of the field intensity in this magnetic field just in time acted on the tube wall position, can control magneticsubstance like this and be enriched in tube wall fully.
Use the base with slide rail shown in Figure 3,5 magnetic separator frames can be fitted together and form a combined magnetic separator frame, its structure as shown in Figure 4, this combined magnetic separator frame can be used for the separation of 10 samples simultaneously.
The 1.5ml centrifuge tube of sample and the user mode of 0.5ml centrifuge tube are housed respectively see Fig. 5 and Fig. 6.
The result shows that the utility model can adsorb magnetic substance effectively.
Embodiment 2
In the present embodiment, in the 1.5ml centrifuge tube, magnetic nanometer composite material is contacted with the liquid sample that contains DNA, form liquid form mixt, wherein said magnetic nanometer composite material has superparamagnetism kernel, middle coated with silica layer and shell, and its diameter is 50-300nm;
Then with centrifuge tube as on the magnetic separator frame among the embodiment 1, the magnetic particle in 1 minute in the pipe will be adsorbed on the one side near the magnet stand center pillar, will abandon liquid then and inhale and go, again with washings washing three times.Obtain being adsorbed with the magnetic nanometer composite material of DNA.
Should be understood that those skilled in the art can make various changes or modifications the utility model after having read above-mentioned teachings of the present utility model, these equivalent form of values fall within the application's appended claims institute restricted portion equally.

Claims (9)

1. assemblnig magnetic separator frame, it comprises following assembly:
At least one magnet stand, described magnet stand comprises: one is provided with the cylinder part of magnet, second pedestal that first pedestal and that is arranged on described cylinder part one end is arranged on the described cylinder part the other end in it, wherein, described first pedestal is provided with at least one first pilot hole, and described second pedestal is provided with second pilot hole that at least one size is different from described first pilot hole;
One base, its be provided with can with first pedestal of described magnet stand and or two slide rails that match in second pedestal.
2. magnetic separator frame as claimed in claim 1 is characterized in that, the diameter of first pilot hole is 7.5 ± 1 millimeters.
3. magnetic separator frame as claimed in claim 1 is characterized in that, the diameter of second pilot hole is 10 ± 2 millimeters.
4. magnetic separator frame as claimed in claim 1 is characterized in that, the number of described magnet stand is 2-100.
5. magnetic separator frame as claimed in claim 1 is characterized in that, the number of described magnet stand is 4-25.
6. magnetic separator frame as claimed in claim 1 is characterized in that, the pilot hole number on first pedestal and second pedestal is 1-5.
7. magnetic separator frame as claimed in claim 1 is characterized in that, described pilot hole be shaped as circle.
8. magnetic separator frame as claimed in claim 1 is characterized in that, the slide rail on the described base all cooperates with first pedestal and second pedestal of described magnet stand.
9. magnetic separator frame as claimed in claim 1 is characterized in that each pilot hole is provided with the V-arrangement baffle plate.
CNU032316925U 2003-05-30 2003-05-30 Assembling magnetic separation bracket with base Expired - Lifetime CN2693770Y (en)

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Application Number Priority Date Filing Date Title
CNU032316925U CN2693770Y (en) 2003-05-30 2003-05-30 Assembling magnetic separation bracket with base

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CN2693770Y true CN2693770Y (en) 2005-04-20

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102174389A (en) * 2011-03-03 2011-09-07 苏州照康生物技术有限公司 Portable multi-functional magnetic separation device
CN102274790A (en) * 2011-05-09 2011-12-14 广州华峰生物科技有限公司 Clamping type magnetic separation frame
CN105665132A (en) * 2014-11-20 2016-06-15 瀚吉康生物科技（北京）有限公司 Combined type magnetic frame
CN105916972A (en) * 2013-12-09 2016-08-31 株式会社百奥尼 Magnetic particle separating device, and method of separating and purifying nucleic acid or protein using same

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102174389A (en) * 2011-03-03 2011-09-07 苏州照康生物技术有限公司 Portable multi-functional magnetic separation device
CN102274790A (en) * 2011-05-09 2011-12-14 广州华峰生物科技有限公司 Clamping type magnetic separation frame
CN102274790B (en) * 2011-05-09 2013-04-24 广州华峰生物科技有限公司 Clamping type magnetic separation frame
CN105916972A (en) * 2013-12-09 2016-08-31 株式会社百奥尼 Magnetic particle separating device, and method of separating and purifying nucleic acid or protein using same
CN105916972B (en) * 2013-12-09 2018-12-21 株式会社百奥尼 The separation and method of purification of magnetic particle separator and nucleic acid or protein using the device
CN105665132A (en) * 2014-11-20 2016-06-15 瀚吉康生物科技（北京）有限公司 Combined type magnetic frame

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C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CX01 Expiry of patent term

Expiration termination date: 20130530

Granted publication date: 20050420