CN2673873Y - Equipment for gas phase culturing strain specially for purifying industrial waste gas - Google Patents

Equipment for gas phase culturing strain specially for purifying industrial waste gas Download PDF

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Publication number
CN2673873Y
CN2673873Y CNU2003201047708U CN200320104770U CN2673873Y CN 2673873 Y CN2673873 Y CN 2673873Y CN U2003201047708 U CNU2003201047708 U CN U2003201047708U CN 200320104770 U CN200320104770 U CN 200320104770U CN 2673873 Y CN2673873 Y CN 2673873Y
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container
gas
waste gas
industrial waste
purifying
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CNU2003201047708U
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Chinese (zh)
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孙珮石
黄若华
郑顺生
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Kunming University of Science and Technology
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Kunming University of Science and Technology
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract

The utility model relates to the equipment for gas phase culturing strain specially for purifying industrial waste gas, which belongs to the technical field of culturing strain purifying waste gas equipment, comprising a closed container with an upper cover, a closed disc type electric heater positioned at the surface of the bottom layer in the container, a stainless steel mesh bracket positioned on the electric heater, a plurality of glass culturing dishes for culturing the strain are positioned on the stainless steel mesh bracket, a gas ingress pipe and a gas discharging pipe which are communicated in the container. A reagent additive pipe which is communicated in the container is also arranged on the container, and a thermometer is also in the upper cover. The utility model has the advantages of simple structure, convenient operation, strong purifying ability of microorganism strain cultured by the utility model for objective pollutant in exhaust gas, strong selecting and utilizing property for the objective pollutant, etc.

Description

The vapor phase process breeding apparatus of industrial waste gas purifying special bacteria
Technical field: the utility model relates to a kind of breeding apparatus of industrial waste gas purifying bacterial classification, is exclusively used in as the vapor phase process of industrial waste gas purifying bacterial classification to cultivate, and belongs to waste-gas cleaning breeding strain device technique field.
Technical background: biological method purification low-concentration industrial waste gas is a kind of novel method of industrial waste gas purifying, the history that extensive studies and application were only had an appointment 20~30 years.Because Biodecontamination reactor relates to processes such as gas, liquid/solid phase mass transfer, bacterial classification performance, microbial film internal diffusion and biochemical degradation, influence factor is many and complicated, though people have carried out many researchs to its basic theory and practical application, but deep not enough, extensive, still have many problems to need further to inquire into constantly perfect.
In biological waste gas purification research both domestic and external in the past, people adopt the bacterial classification of having used in the field of waste water treatment for many years more, after liquid phase is cultivated domestication with pending target contaminant, be directly used in the purifying treatment of waste gas, but often be difficult to reach the decontamination effect improving of expection.Technology and the special-purpose breeding apparatus of cultivating domestication waste-gas cleaning special microorganism strain with vapor phase process do not appear in the newspapers as yet.
Summary of the invention: the purpose of this utility model is at above-mentioned situation, a kind of biopurification process mechanism research based on the volatile organic matter VOC that is insoluble in water common in the low-concentration industrial waste gas, sulfide (as hydrogen sulfide, dithiocarbonic anhydride), pollutents such as NOx, SO2 is provided, utilizes present organic matter degradation bacteria commonly used in waste water treatment engineering, denitrifier, thiobacterium etc. that the waste-gas cleaning special microorganism strain is carried out the device that vapor phase process is cultivated.
The technical solution of the utility model is: (it can be made by glass or stainless material the vapor phase process breeding apparatus of this industrial waste gas purifying special bacteria by the airtight cylindrical or square column type container 1 of being with loam cake 7, between loam cake and housing for being tightly connected), be positioned at the surperficial airtight disc type electric heater 4 of container bottom and stainless (steel) wire support 3 placed on it (well heater and spawn culture ware are separated), level places a plurality of conventional microbiology experimental strain on the stainless (steel) wire support 3 to cultivate with glass culture dish 2 (its quantity can be determined according to the actual volume size of container 1, be advisable for general 7~12), and the gas introduction tube 6 and the vapor pipe 5 that feed in the container 1 are formed.
Also can have thermometer 8 on the loam cake 7 in this container, be used to indicate temperature in the container.When being lower than the normal temperature scope, can heat by electric heater 4; Also be provided with another reagent that feeds in it on the container 1 and add pipe 9.When certain performance of the bacterial classification of cultivating in order to increase or to strengthen, can add pipe 9 by this reagent and in this breeding apparatus container, inject necessary additive.For example when cultivating toluene waste gas and purify bacterial classification, can add gases such as homologue benzene that pipe 9 adds toluene, dimethylbenzene by this reagent, with strengthen the bacterial classification of being cultivated to performances such as the adaptability of toluene homologue in the purifying exhaust air, comprehensive purifying abilities.Again for example when cultivating NOx or SO2 waste-gas cleaning bacterial classification, in the time of can adding pipe 9 and add the denitrifier cultivated or desulfurization bacterium growth by this reagent in needed carbon compound (carbon source), other necessary substrate source material and the waste gas with other gaseous pollutant (as itrogenous organic substance or organic sulfide) of NOx or SO2 coexistence.
Be full of gaseous state target contaminant (as toluene gas) and AIR MIXTURES in this cultivating container, do not contain the main element relevant in the culture medium flat plate of glass culture dish (when purifying bacterium as cultivating toluene with target contaminant, carbon elements not in the culture medium flat plate), container does not have liquid water yet and imports and discharge simultaneously.In this environment, the microbial strains of being cultivated (being coated on the culture medium flat plate surface in advance) can only be used the matrix of the gaseous state target contaminant in the gas phase as its growth and breeding.
That the utility model can utilize in the present waste water treatment engineering is commonly used, the different target pollutent is had the microbial strains of degraded cleaning action, cultivates domestication by special vapor phase process and obtains the industrial waste gas purifying special microorganism strain.Have simple in structure easy to operate, obviously be better than adopting in the wastewater treatment conventional liquid phase to cultivate method for domesticating gained bacterial classification, target contaminant is selected to utilize advantages such as performance is strong with the microbial strains of its cultivation to the detergent power of the target contaminant in the waste gas.
Description of drawings: below in conjunction with accompanying drawing, be further described, but the utility model protection domain is not limit embodiment with regard to the utility model structure.
Fig. 1 is the utility model cross-sectional schematic.
Fig. 2 bows for the utility model and cuts open synoptic diagram.
Embodiment: below in conjunction with drawings and Examples essence of the present utility model is further specified.
Embodiment 1: as shown in Figure 1, 2, the vapor phase process breeding apparatus of this industrial waste gas purifying special bacteria is that the airtight cylindrical glass container 1 (being to be tightly connected between its loam cake and housing) of band loam cake 7, the surperficial airtight disc type electric heater 4 that is positioned at the container bottom and stainless (steel) wire support 3, level placed on it place 7 conventional microbiology experimental strains cultivations on the stainless (steel) wire support 3 to form with the gas introduction tube 6 and the vapor pipe 5 of glass culture dish 2 and feeding container 1 by outward appearance.
Also can have thermometer 8 on the loam cake 7 in this container, be used to indicate temperature in the container, when being lower than the normal temperature scope, can heat by electric heater 4; Also be provided with another reagent that feeds in it on the container 1 and add pipe 9, be used to add the homologue of target contaminant, with strengthen the bacterial classification of being cultivated to performances such as the adaptability of target contaminant in the purifying exhaust air and homologue, comprehensive purifying abilities.
Embodiment 2: as shown in Figure 1, 2, the vapor phase process breeding apparatus of this industrial waste gas purifying special bacteria by outward appearance be band loam cake 7 airtight stainless steel square cylindrical container 1 (between its loam cake and housing for be tightly connected), be positioned at the surperficial airtight disc type electric heater 4 of container bottom and stainless (steel) wire support 3 placed on it (well heater and spawn culture ware are separated), level places 12 conventional microbiology experimental strains cultivations on the stainless (steel) wire support 3 to form with the gas introduction tube 6 and the vapor pipe 5 of glass culture dish 2 and feeding container 1.
Also can have thermometer 8 on the loam cake 7 in this container, be used to indicate temperature in the container, when being lower than the normal temperature scope, can heat by electric heater 4.Also be provided with another reagent that feeds in it on the container 1 and add pipe 9.When certain performance of the bacterial classification of cultivating in order to increase or to strengthen, can add pipe 9 by this reagent and in this breeding apparatus container, inject necessary additive.For example when cultivating toluene waste gas and purify bacterial classification, can add gases such as homologue benzene that pipe 9 adds toluene, dimethylbenzene by this reagent, with strengthen the bacterial classification of being cultivated to performances such as the adaptability of toluene homologue in the purifying exhaust air, comprehensive purifying abilities.Again for example when cultivating NOx or SO2 waste-gas cleaning bacterial classification, in the time of can adding pipe 9 and add the denitrifier cultivated or desulfurization bacterium growth by this reagent in needed carbon compound (carbon source), other necessary substrate source material and the waste gas with other gaseous pollutant (as itrogenous organic substance or organic sulfide) of NOx or SO2 coexistence.
The working method of using of the present utility model is (being example to cultivate toluene waste gas purification bacterium):
The first step is selected substratum at target contaminant toluene preparation, also promptly prepares not carbonaceous sources, but contains the selective medium of needed other matrix components of microbial strains growth (as nitrogen phosphorus etc.).And microbiology experimental technique routinely, with agar selective medium is made into the special-purpose no carbon source culture dish 2 that solidifies.
With screening from associated biomolecule method waste disposal plant in advance and through experimental verification target contaminant toluene being had the strain liquid of Degradation, the microbiology experimental technique is coated with inoculation to above-mentioned culture dish flat board 2 routinely.
Second step, several culture dish flat boards 2 that are coated with the preliminary election bacterial classification are put into container 1 and built loam cake 7, feed gaseous state target contaminant toluene from gas introduction tube 6 then and cultivate domestication; When from vapor pipe 5 expellant gas, when the concentration of gaseous state target contaminant (toluene gas) reaches desired certain limit, promptly stop air inlet and exhaust, and close the valve on inlet pipe and the vapor pipe, this moment, the gaseous state target contaminant was full of space in the entire container.
When cultivating toluene waste gas purification special bacteria, be full of gaseous state target contaminant toluene gas and AIR MIXTURES in this breeding apparatus, no liquid water imports and discharges.Vapor phase toluene concentration is 3~5 times of toluene concentration in the general industry waste gas in the apparatus container.Inoculation is coated on special-purpose microbial strains (as pseudomonas) of solidifying no carbon source culture medium flat plate surface and can only utilizes toluene gas in the gas phase as the sole carbon source (substrate source) of its growth.
The cultivation domestication operation of bacterial classification is all carried out under normal temperature and pressure conditions.When microbial strains utilizes toluene in the gas phase, and, also promptly realized the mechanism of the bacterial classification in the microbial film to the absorption-degraded-purification of toluene in the gas phase solidifying substratum 2 planar surfaces with the constantly growth and when progressively forming microbial film of bacterium colony form.By the fraction of coverage of observing and the measuring and calculating different time solidifies culture medium flat plate surface biological film, can judge the upgrowth situation of the bacterial classification on this flat board.
When a thermometer indicates temperature in the container when being lower than the normal temperature scope, with disc type electric heater 4 heating that are arranged on the bottom.Cultivate bacterial classification in order to increase or to strengthen to performances such as the adaptability of the toluene homologue that coexists in the purifying exhaust air, comprehensive purifying abilities, can add pipe 9 by reagent and in breeding apparatus, inject necessary additives (as the homologue benzene of toluene, dimethylbenzene gas etc.).
In the 3rd step, when observing the biomembranous fraction of coverage of dull and stereotyped 2 surface colonys of culture dish when reaching 30~60%, promptly microbiology experimental technique routinely carries out the operation of selective advantage bacterial classification from dominant colony.The selected dominant bacteria that goes out is exactly the microbial strains that gaseous state target contaminant toluene is had special degraded cleaning action.
Purifying property for the gaseous state target contaminant of strengthening bacterial classification, generally to carry out once more the dull and stereotyped coating of special culture media by the dominant bacteria that aforesaid operations obtains, cultivate domestication, dominant bacteria once more with breeding apparatus and operation such as select, and repetitive operation 3 times successively.
The 4th step repeated to cultivate the dominant bacteria that domestication obtains through 3 deuterzooid breeding apparatus, was final toluene waste gas and purified special microorganism strain, can adopt conventional micro-biological process to preserve or use after breeding.
If cultivate other indissoluble (or insoluble) common in the purifying low-concentration industrial gaseous waste when the special bacteria of the volatile organic matter (as benzene, dimethylbenzene, vinylbenzene, formaldehyde etc.) of water, sulfide (as hydrogen sulfide, dithiocarbonic anhydride etc.), pollutents such as NOx, SO2, as long as target contaminant is replaced by the pollutent that other need be handled by toluene.The gas phase target pollutant concentration is 3~5 times of corresponding Pollutant levels in the general industry waste gas in the breeding apparatus.For pollutent nitrogenous, sulphur, then need when the preparation selective medium, note not containing nitrogen or element sulphur.

Claims (3)

1, a kind of vapor phase process breeding apparatus of industrial waste gas purifying special bacteria is characterized in that placing a plurality of conventional microbiology experimental strain cultivation on the stainless (steel) wire support (3) to form with the gas introduction tube (6) and the vapor pipe (5) of glass culture dish (2) and feeding container (1) by airtight cylindrical or square column type container (1), the surperficial airtight disc type electric heater (4) that is positioned at the container bottom and stainless (steel) wire support (3), the level placed on it of band loam cake (7).
2, the vapor phase process breeding apparatus of industrial waste gas purifying special bacteria according to claim 1 is characterized in that also can having thermometer (8) on the loam cake (7) of container.
3, the vapor phase process breeding apparatus of industrial waste gas purifying special bacteria according to claim 1 and 2 is characterized in that also can having on the container (1) another reagent that feeds in it to add pipe (9).
CNU2003201047708U 2003-12-29 2003-12-29 Equipment for gas phase culturing strain specially for purifying industrial waste gas Expired - Fee Related CN2673873Y (en)

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CNU2003201047708U CN2673873Y (en) 2003-12-29 2003-12-29 Equipment for gas phase culturing strain specially for purifying industrial waste gas

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105918261A (en) * 2016-07-15 2016-09-07 云南中烟工业有限责任公司 Determination device and determination method for influence of gas phase substance on motion capability of nematodes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105918261A (en) * 2016-07-15 2016-09-07 云南中烟工业有限责任公司 Determination device and determination method for influence of gas phase substance on motion capability of nematodes

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