CN2657002Y - Checking device for organic phosphorus pesticide - Google Patents
Checking device for organic phosphorus pesticideInfo
- Publication number
- CN2657002Y CN2657002Y CN 03271481 CN03271481U CN2657002Y CN 2657002 Y CN2657002 Y CN 2657002Y CN 03271481 CN03271481 CN 03271481 CN 03271481 U CN03271481 U CN 03271481U CN 2657002 Y CN2657002 Y CN 2657002Y
- Authority
- CN
- China
- Prior art keywords
- enzyme sensor
- substrate
- pick
- injection device
- water sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A detecting device of organophosphorus pesticides comprises sampling devices of cleaner water and water samples and an enzyme sensor computer automatic control device formed by a recognition element and a PH electrode. The utility model is characterized in that the detecting device also comprises a substrate injection device and an enzyme sensor activity recovery device that are respectively communicated with the recognition element and a peristaltic pump. The sampling devices are a cleaner water vessel and a water sample vessel that are respectively trigged by correspondent electromagnetic valves and communicated with the peristaltic pump by a three way. The substrate injection device and the activity recovery device respectively consist of a substrate vessel for containing substrate solution, an energy rehabilitation agent vessel for containing energy rehabilitation agents and correspondent metering pumps and are connected with the recognition element, that is, the enzyme sensor. The sampling devices, the substrate injection device, the activity recovery device and the enzyme sensor are communicated with each other and controlled by the computer automatic control device to form a controlled flow-injection device. Through the utility model, organophosphorus pesticides in water can be directly and continuously detected.
Description
Technical field
The utility model relates to a kind of pick-up unit to organophosphorus pesticides such as the parathion-methyl in the water body, malathion, DDVP.
Background technology
At present, the monitoring technology of organophosphorus pesticide comprises the chromatography that the observation of watching reagent or test paper change color and exact instrument are measured.The former is directly perceived, quick, but owes quantitative or digitizing; Latter's measurement result accuracy height, but used instrument is bigger complicated pretreatment such as need extract, concentrate and operate, and be not easy to on-the-spot express-analysis, and price is higher, is unfavorable for the analysis of a large amount of samples.For this reason, existing patented claim discloses " a kind of on-the-spot method and instrumentation thereof that detects organophosphorus pesticide " (number of patent application: 99126615.3), for utilizing responsive biology sensor and comprising that the automaton of hardware and softwares such as data acquisition, cpu central processing unit carries out the scene detection, has stepped a gratifying step.But its sensitivity is low, for improving the sensitivity of instrument, needs to add solid oxidizer to strengthen the toxicity of organophosphorus pesticide in sample, with solid reductant excess oxidant is reduced again.In addition, also need manually to add sample, this has limited the automatic working level of instrument widely.Do not see at present the report that this instrument is used for detecting the micro-organophosphorus pesticide of seawater yet.
Summary of the invention
The purpose of this utility model is the deficiency that overcomes prior art, and a kind of the water body particularly pick-up unit of organophosphorus pesticide and its critical component---enzyme sensor in the seawater of detecting is provided.
The utility model comprises enzyme sensor that sampling device, recognition component and the pH electrode of cleaning water sample and water sample to be measured constitute and the computer controlled automatic device that comprises software program, it is characterized in that it also comprises substrate injection device and the enzyme sensor activation recovering device that communicates with recognition component and peristaltic pump respectively.Above-mentioned sampling device is respectively the cleaning water sample container and the water sample container to be measured of corresponding solenoid valve braking, and communicates with peristaltic pump via a threeway.Substrate injection device and activation recovering device then are made up of the substrate container of splendid attire substrate solution, the reactivation agent container and the corresponding measuring pump of splendid attire reactivation agent respectively, and with recognition component be that enzyme sensor joins.Above-mentioned sampling device, substrate injection device, activation recovering device are connected with enzyme sensor, and are subjected to the control of computer controlled automatic device and constitute controlled flowing injection device.Described enzyme sensor can and have the integral structure that the cell of electrode is formed by recognition component.
The utility model is combined with enzyme sensor and peristaltic pump, volume pump, solenoid valve and computer controlled automatic device, by the switching of the existing procedure auto-control peristaltic pump of setting, solenoid valve, volume pump and calculating, demonstration pesticide concentration, reach auto injection and substrate and inject, make continuous detecting or online detection become possibility.After a sample determination finished, the activity of the immobilised enzymes that is suppressed can very fast recovery under the situation of not changing recognition component, can be carried out the mensuration of next sample by injecting the reactivation agent, and the analysis quality of subsequent sample is guaranteed.
After earlier will clean water sample and pump into the enzyme sensor certain hour when promptly measuring, inject the substrate solution of tens μ L more simultaneously by the volume pump pulse, generation control signal peak by peristaltic pump; Pump into water sample to be measured then equally and produce the sample signal peak, all calculate organophosphorus pesticide concentration and also show by computer stored and the existing calculation procedure of basis; Before measuring next water sample to be measured, after cleaning water sample certain hour re-entered by peristaltic pump in elder generation, the reactivation agent of carrying another volume pump injection more simultaneously entered enzyme sensor, continues the mensuration of next water sample to be measured after the activity of recovery immobilised enzymes again.
Description of drawings
Fig. 1 is a general structure synoptic diagram of the present utility model
Fig. 2 is an enzyme sensor structural representation of the present utility model
Wherein, 1, cleaning water sample container 2, water sample container 3 to be measured, substrate container 4, reactivation agent container 5,6, solenoid valve 7, threeway 8, peristaltic pump 9,10, volume pump 11, enzyme sensor 12, computer controlled automatic device 13, tubing string 14, immobilised enzymes 15, enzyme-blocking layer 16, recognition component 17, electrode 18, cell D, injection port E, waste liquid outlet
Embodiment
The sampling device, recognition component 16 that the utility model comprises cleaning water sample and water sample to be measured and the enzyme sensor 11 of pH electrode 17 formations and the computer controlled automatic device 12 that comprises software program (are seen Fig. 1, solid line is represented sample or flow of solution pipeline, dotted line represents to control automatically route), it is characterized in that it also comprises substrate injection device and the enzyme sensor activation recovering device that communicates with recognition component 16 and peristaltic pump 8 respectively.Above-mentioned sampling device is respectively the cleaning water sample container 1 and the water sample container 2 to be measured of corresponding solenoid valve 5,6 brakings, and communicates with peristaltic pump 8 via a threeway 7.Substrate injection device and activation recovering device then are made up of the substrate container 3 of splendid attire substrate solution, the reactivation agent container 4 and the corresponding measuring pump 9,10 of splendid attire reactivation agent respectively, and with recognition component 16 be that enzyme sensor 11 joins.Above-mentioned sampling device, substrate injection device, activation recovering device are connected with enzyme sensor, and are subjected to the control of computer controlled automatic device 12 and constitute controlled flowing injection device.
Substrate solution in the substrate container 3 is an acetylthiocholine iodide solution, adds flowing injection device by computer controlled automatic device 12 control volume pumps 9.Substrate solution is advisable with the acetylthiocholine iodide solution of 25-50g/L.
After testing sample was measured and finished, 10 work of computer controlled automatic device 12 control volume pumps added 20-100 μ L reactivation agent solution from reactivation agent container 4 to the tubing string 13 of recognition component 16.The reactivation agent solution is advisable with the acetylthiocholine iodide aqueous solution of 70-100g/L.
The integral structure that enzyme sensor 11 is made up of recognition component 16 and the cell 18 that has electrode 17, promptly recognition component 16 and electrode 17 place cell 18, and both parallel distances are less than 1.5cm.Electrode 17 is advisable with E-201-C type pH combination electrode.
The recognition component 16 of enzyme sensor 11 is structures that are marked with immobilised enzymes 14 in tubing string 13.For preventing the loss of immobilised enzymes 14 in tubing string 13, enzyme-blocking layer 15 is arranged below immobilised enzymes 14, enzyme-blocking layer 15 should be selected 300-400 purpose bolting silk or glass fibre for use.
Immobilised enzymes 14 in the above-mentioned tubing string 13 is to be the acetylcholinesterase of carrier with the gel.Can just select the addition of immobilised enzymes 14 according to pesticide concentration, if measure the water sample of organophosphorus pesticide concentration less than 10 μ g/L, the addition of immobilised enzymes is 0.5-1U; If measure the water sample of organophosphorus pesticide concentration greater than 10 μ g/L, then the addition of immobilised enzymes is 2-5U.
The micro-organophosphorus pesticide of measuring in the seawater with this device is an example.Its determination step is as follows:
(1) will clean water sample by peristaltic pump earlier and be continuously pumped into enzyme sensor, continue 20min.Under the situation that the water sample that keeps clean is continuously pumped into, inject 40 μ L substrate solutions by the volume pump pulse, enter enzyme sensor with the cleaning water sample, produce the control signal peak, by computer recording.
(2) import testing sample 20min by peristaltic pump continuously to enzyme sensor, same then step (1) is injected 40 μ L substrate solutions by the volume pump pulse, enters enzyme sensor with testing sample, produces the sample signal peak, by computer stored.
(3) computing machine calculates the inhibiting rate of testing sample to the enzyme sensor activity according to existing program, according to the sample salinity and the temperature of input in advance, the concentration that is calculated organophosphorus pesticide in the sample by corresponding organophosphorus pesticide concentration and enzymatic activity inhibiting rate relation equation in the existing program also shows again.
(4) peristaltic pump re-enters cleaning water sample 20min, when 10min, adds the reactivation agent solution by another volume pump, enters enzyme sensor with the cleaning water sample, can recover the activity of immobilised enzymes.
(5) set by step the mensuration of next testing sample is carried out in (2), (3).
The condition that the utility model is suitable for is that concentration of suspension should be lower than 30mg/L in institute's test sample product.
Use the utility model, can continuously, directly measure, concentrate or add oxygenant and improve preprocessing process such as organophosphorus pesticide toxicity and need not to carry out enrichment to organophosphorus pesticides such as the parathion-methyl in the water body, malathion, DDVP.Finding speed is about the 30min/ sample.
Claims (8)
1, the pick-up unit of organophosphorus pesticide, it comprises the sampling device of cleaning water sample and water sample to be measured, recognition component (16) and the enzyme sensor (11) of pH electrode (17) formation and the computer controlled automatic device (12) that comprises software program, it is characterized in that it also comprises substrate injection device and the enzyme sensor activation recovering device that communicates with recognition component (16) and peristaltic pump (8) respectively, above-mentioned sampling device is respectively corresponding solenoid valve (5), (6) Zhi Dong cleaning water sample container (1) and water sample container to be measured (2), and communicate with peristaltic pump (8) via a threeway (7), substrate injection device and activation recovering device are then respectively by the substrate container (3) of splendid attire substrate solution, the reactivation agent container (4) of splendid attire reactivation agent and corresponding measuring pump (9), (10) form, and with recognition component (16) be that enzyme sensor (11) joins; Above-mentioned sampling device, substrate injection device, activation recovering device are connected with enzyme sensor, and are subjected to the control of computer controlled automatic device (12) and constitute controlled flowing injection device.
2, pick-up unit as claimed in claim 1 is characterized in that the reactivation agent solution in the reactivation agent container (4) is an acetylthiocholine iodide solution.
3, pick-up unit as claimed in claim 1 is characterized in that enzyme sensor (11) is by recognition component (16) with have the integral structure that the cell (18) of electrode (17) is formed.
4, as claim 1 or 3 described pick-up units, the recognition component (16) that it is characterized in that enzyme sensor (11) is a structure that is marked with immobilised enzymes (14) in tubing string (13).
5, pick-up unit as claimed in claim 4 is characterized in that the immobilised enzymes (14) in the above-mentioned tubing string (13) is to be the acetylcholinesterase of carrier with the gel.
6, pick-up unit as claimed in claim 4 is characterized in that in the below of immobilised enzymes (14) enzyme-blocking layer (15) being arranged.
7, pick-up unit as claimed in claim 6 is characterized in that enzyme-blocking layer (15) should select 300-400 purpose bolting silk or glass fibre for use.
8,, it is characterized in that electrode (17) is an E-201-C type pH combination electrode as claim 1 or 3 described pick-up units.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03271481 CN2657002Y (en) | 2003-08-18 | 2003-08-18 | Checking device for organic phosphorus pesticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03271481 CN2657002Y (en) | 2003-08-18 | 2003-08-18 | Checking device for organic phosphorus pesticide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2657002Y true CN2657002Y (en) | 2004-11-17 |
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ID=34330743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03271481 Expired - Fee Related CN2657002Y (en) | 2003-08-18 | 2003-08-18 | Checking device for organic phosphorus pesticide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2657002Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100376890C (en) * | 2003-08-18 | 2008-03-26 | 中国海洋大学 | Detecting method of organic phosphorus pesticide and its device |
| CN100380152C (en) * | 2003-11-07 | 2008-04-09 | 长飞光纤光缆有限公司 | Optical cable laid in sewer line and laying method thereof |
-
2003
- 2003-08-18 CN CN 03271481 patent/CN2657002Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100376890C (en) * | 2003-08-18 | 2008-03-26 | 中国海洋大学 | Detecting method of organic phosphorus pesticide and its device |
| CN100380152C (en) * | 2003-11-07 | 2008-04-09 | 长飞光纤光缆有限公司 | Optical cable laid in sewer line and laying method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |