CN2565838Y - New type bioreactor of tissue engineering skin in vitro structure - Google Patents
New type bioreactor of tissue engineering skin in vitro structure Download PDFInfo
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- CN2565838Y CN2565838Y CN02266979U CN02266979U CN2565838Y CN 2565838 Y CN2565838 Y CN 2565838Y CN 02266979 U CN02266979 U CN 02266979U CN 02266979 U CN02266979 U CN 02266979U CN 2565838 Y CN2565838 Y CN 2565838Y
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- perfusion
- nutrient solution
- reactor
- loam cake
- skin
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Abstract
The utility model relates to a novel bioreactor of tissue engineering skin in vitro structure. A bioreactor main body and an upper cover form a closed container. The bioreactor main body and the upper cover form a perfusion chamber, wherein, the bottom of which is provided with a supporting circular table which is convex upwards. The middle part of the supporting circular table is provided with an air pressure chamber. Complexes used for building a skin are covered on the air pressure chamber and the supporting circular table, and are fixed through a fastening sealing component, wherein, the periphery of which is provided with a culture liquid channel, wherein, the periphery of which is provided with a liquid level control table, wherein, the circumference of which is provided with a liquid storage tank. A gas space is formed among the upper cover, the supporting circular table and the liquid level control table. The upper cover is provided with an upper perfusion channel communicated with the perfusion chamber. The utility model has the advantages of simple structure and convenient operation. The asepsis micro environment can be kept for a long time. After the utility model is combined, the utility model can be conveniently arranged in a CO#-< 2 > incubator, which provides a relative closing, nontoxic, sterile environment of sufficient oxygen supply and stable temperature for building the skin. A growing environment of a human body can be simulated by the utility model.
Description
Technical field
The utility model relates to a kind of bio-reactor, relates in particular to the bio-reactor of external structure skin in a kind of medical science and the organizational engineering.
Background technology
As everyone knows, skin is the tissue of human body maximum, is the barrier that contacts with external environment.When causing skin injury owing to factors such as extraneous damage or diseases, its harm can be extremely slight, also can be fatal.
Before the history of skin injury reparation can be traced back to hundreds of years, reparation approach at present commonly used has: from body skin-grafting, allograft of skin, xenogenesis skin-grafting, but, seek a kind of ideal Graftskin and be always difficult problem of needing solution badly clinically owing to, immunological rejection not enough and reason such as spread disease for the district.
Organizational engineering is the principle and the method for application project and life science, makes up biological surrogate, to recover, to keep or promote a science of impaired organ or tissue function.Proposing artificial constructed Graftskin according to the principle of organizational project is a kind of efficient ways.According to classification commonly used, tissue engineering skin can be divided into epiderm substitute (epidermal replacement), dermal substitute (dermal replacementS) and composite skin surrogate (composite skinreplacement).Wherein there are deficiency more or less in epiderm substitute, dermal substitute in practical clinical, therefore, seek a kind of Composite Skin surrogate truly in the hope of being further improved on function and profile, will be the targets that people pursue.
Make up above-mentioned Composite Skin surrogate truly, must make the fibroblastic cell complexes that contains in epidermis epithelial cells and the corium place liquid-vapo(u)r interface, so that the nutritive substance in itself and the nutrient solution keeps excellent contact, simulated skin growth in vivo environment, to impel epithelial cells to be differentiated to form stratum corneum, realize the further improvement on function and the profile.Therefore, the bio-reactor of external structure skin will be an important key factor of decision Composite Skin surrogate performance.MINUCELLS AND MINUTISSUE has disclosed a kind of bio-reactor of external structure skin, this reactor is complex structure not only, and can't make cell complexes at simulated skin growth in vivo environment, thereby the Composite Skin surrogate performance that is obtained is lacked perfection.
Summary of the invention
The utility model technical issues that need to address are a kind of bio-reactors that are used for the skin external structure of design, to overcome complex structure that prior art exists, can't make the shortcoming of cell complexes at simulated skin growth in vivo environment.
The technical solution of the utility model:
The closed container of reactor of the present utility model for constituting by reactor body and loam cake, reactor body and loam cake constitute the perfusion cell, perfusion cell bottom is provided with the support round platform to upper process, supporting the round platform middle part is air-pressure chamber, air-pressure chamber and gas go out, inlet is connected, the pellosil and the cell-material composite that are used to make up skin are covered in air-pressure chamber and support round platform, and fix by fit sealing spare, the periphery of fit sealing spare is the nutrient solution passage, the nutrient solution passage links to each other mutually with the nutrient solution inlet, the periphery of nutrient solution passage is the tank level control platform, is reservoir around the tank level control platform, and reservoir is connected with the nutrient solution outlet.Form a gas space between loam cake and support round platform and the tank level control platform.
On be covered with the last perfusion passage that is connected with the perfusion cell, this passage is connected with last perfusion inlet.
According to the utility model, can in the closed container of reactor body and loam cake formation, a plurality of separate perfusion cells be set, the reservoir of each perfusion cell is interconnected.
The utility model is to use like this: the pellosil and the cell-material composite that are used to make up skin are covered in air-pressure chamber and support round platform, and fix by fit sealing spare, with nutrient solution from nutrient solution inlet injecting reactor perfusion cell, for the cell complexes that is covered on the support platform provides nutrient, the height of the tank level control platform may command nutrient solution liquid level of perfusion cell, in the reservoir around unnecessary nutrient solution can flow into, guarantee the formation of liquid-vapo(u)r interface, last perfusion passage can be regular be that cell complexes surface drips nutrient solution, assurance is in airborne cell and can grows normally, breeding, differentiation, nutrient solution is flowed out by the nutrient solution outlet, can be recycled, pressurized air can enter air-pressure chamber by the gas inlet, make to be covered in being used on the air-pressure chamber and to make up the pellosil of skin and cell-material composite and be subjected to tensile and stimulate, with the growing environment of simulation human body.
The utility model is simple in structure, be convenient to operation, can keep aseptic subenvironment for a long time, can conveniently insert after the combination in the CO2 incubator,, and can simulate the growing environment of human body for the structure of skin provides the environment of relative closure, the confession of nontoxic, aseptic, sufficient oxygen, equilibrium temperature.
Description of drawings
Fig. 1 is the utility model skin reaction device one-piece construction synoptic diagram.
Fig. 2 is that A---A among Fig. 1 is to synoptic diagram.
Fig. 3 is that B---B among Fig. 1 is to synoptic diagram.
Embodiment
Referring to Fig. 1, Fig. 2 and Fig. 3, the closed container of reactor of the present utility model for constituting by reactor body 2 and loam cake 3, reactor body 2 constitutes four independently perfusion cells 13 with loam cake 3, perfusion cell 13 bottoms are provided with the support round platform 21 to upper process, supporting round platform 21 middle parts is air-pressure chamber 5, air-pressure chamber 5 is by pressure channel 22 and pneumatic outlet 14, inlet 10 is connected, being used to make up the pellosil 20 of skin and cell-material composite covers 7 and is placed on air-pressure chamber 5 and supports round platform 21, and it is fixing by fit sealing spare 6, the periphery of fit sealing spare 6 is the ring-like passage 23 of nutrient solution, the ring-like passage 23 of nutrient solution links to each other mutually with nutrient solution inlet 1 by nutrient solution access road 24, the periphery of the ring-like passage 23 of nutrient solution is a tank level control platform 17, around the tank level control platform 17 is reservoir 16, and this reservoir 16 is connected with nutrient solution outlet 8.Form a gas space 4 between loam cake 3 and support round platform 21 and the tank level control platform 17.
The fit sealing spare 6 preferred wear rings of being addressed that adopt.
Loam cake 3 is provided with the last perfusion passage 19 that is connected with perfusion cell 13, and this passage 19 is connected with last perfusion inlet 28.
Reactor body (2) cooperates fixing with loam cake (3) by removable pin (9), locking pin (11) and lock cover (12).
Claims (7)
1. the new-type bioreactor of organization engineering skin external structure, it is characterized in that reactor is the closed container that is made of reactor body (2) and loam cake (3), reactor body (2) constitutes perfusion cell (13) with loam cake (3), perfusion cell (13) bottom is provided with the support round platform (21) to upper process, supporting round platform (21) middle part is air-pressure chamber (5), air-pressure chamber (5) is by pressure channel (22) and pneumatic outlet (14), inlet (10) is connected, being used to make up the pellosil (20) of skin and cell-material composite covers (7) and is placed on air-pressure chamber (5) and supports round platform (21), and it is fixing by fit sealing spare (6), the periphery of fit sealing spare (6) is the ring-like passage of nutrient solution (23), the ring-like passage of nutrient solution (23) links to each other mutually with nutrient solution inlet (1) by nutrient solution access road (24), the periphery of the ring-like passage of nutrient solution (23) is tank level control platform (17), around the tank level control platform (17) is reservoir (16), this reservoir (16) is connected with nutrient solution outlet (8), form a gas space (4) between loam cake (3) and support round platform (21) and the tank level control platform (17), loam cake (3) is provided with the last perfusion passage (19) that is connected with perfusion cell (13), this passage (19) and last perfusion enter the mouth (28) be connected.
2. reactor according to claim 1 is characterized in that, in the closed container that reactor body (2) and loam cake (3) constitute a plurality of separate perfusion cells (13) is set, and the reservoir (16) of each perfusion cell (13) is interconnected.
3. reactor according to claim 2 is characterized in that, in the closed container that reactor body (2) and loam cake (3) constitute four separate perfusion cells (13) is set.
4. reactor according to claim 1 is characterized in that the fit sealing spare of being addressed (6) preferably adopts wear ring.
5. reactor according to claim 2 is characterized in that the fit sealing spare of being addressed (6) preferably adopts wear ring.
6. reactor according to claim 3 is characterized in that the fit sealing spare of being addressed (6) preferably adopts wear ring.
7. according to each described reactor of claim 1～6, it is characterized in that reactor body (2) cooperates fixing with loam cake (3) by removable pin (9), locking pin (11) and lock cover (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN02266979U CN2565838Y (en) | 2002-09-09 | 2002-09-09 | New type bioreactor of tissue engineering skin in vitro structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN02266979U CN2565838Y (en) | 2002-09-09 | 2002-09-09 | New type bioreactor of tissue engineering skin in vitro structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2565838Y true CN2565838Y (en) | 2003-08-13 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN02266979U Expired - Fee Related CN2565838Y (en) | 2002-09-09 | 2002-09-09 | New type bioreactor of tissue engineering skin in vitro structure |
Country Status (1)
| Country | Link |
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| CN (1) | CN2565838Y (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985598A (en) * | 2010-11-08 | 2011-03-16 | 中国人民解放军第三军医大学第三附属医院 | Dynamic three-dimensional strain culture device for tissue-engineered skin |
| CN101402917B (en) * | 2008-11-25 | 2011-07-06 | 中国人民解放军第三军医大学第三附属医院 | Bioreactor for producing tissue engineering products |
| CN101333498B (en) * | 2008-06-13 | 2011-11-23 | 东华大学 | Apparatus for culturing tissue engineered blood |
| CN102618442A (en) * | 2012-04-06 | 2012-08-01 | 中国人民解放军第四军医大学 | Artificial skin culture device and using method thereof |
| CN103740591A (en) * | 2014-01-17 | 2014-04-23 | 康珞生物科技（武汉）有限公司 | Three-dimensional perfusion type cell culture device and application thereof |
| CN105176815A (en) * | 2015-10-20 | 2015-12-23 | 哈尔滨工业大学 | Gas-liquid interface perfusion bioreactor for artificial skin tissue culture and preparation method and application method of gas-liquid interface perfusion bioreactor |
| CN105255732A (en) * | 2015-11-10 | 2016-01-20 | 杭州电子科技大学 | Tissue engineered cartilage in-vitro culture device |
-
2002
- 2002-09-09 CN CN02266979U patent/CN2565838Y/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101333498B (en) * | 2008-06-13 | 2011-11-23 | 东华大学 | Apparatus for culturing tissue engineered blood |
| CN101402917B (en) * | 2008-11-25 | 2011-07-06 | 中国人民解放军第三军医大学第三附属医院 | Bioreactor for producing tissue engineering products |
| CN101985598A (en) * | 2010-11-08 | 2011-03-16 | 中国人民解放军第三军医大学第三附属医院 | Dynamic three-dimensional strain culture device for tissue-engineered skin |
| CN102618442A (en) * | 2012-04-06 | 2012-08-01 | 中国人民解放军第四军医大学 | Artificial skin culture device and using method thereof |
| CN103740591A (en) * | 2014-01-17 | 2014-04-23 | 康珞生物科技（武汉）有限公司 | Three-dimensional perfusion type cell culture device and application thereof |
| CN103740591B (en) * | 2014-01-17 | 2015-07-15 | 康珞生物科技（武汉）有限公司 | Three-dimensional perfusion type cell culture device and application thereof |
| CN105176815A (en) * | 2015-10-20 | 2015-12-23 | 哈尔滨工业大学 | Gas-liquid interface perfusion bioreactor for artificial skin tissue culture and preparation method and application method of gas-liquid interface perfusion bioreactor |
| CN105255732A (en) * | 2015-11-10 | 2016-01-20 | 杭州电子科技大学 | Tissue engineered cartilage in-vitro culture device |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |