CN2518106Y - Synchronous enzyme immunity analyzer - Google Patents
Synchronous enzyme immunity analyzer Download PDFInfo
- Publication number
- CN2518106Y CN2518106Y CN 00215156 CN00215156U CN2518106Y CN 2518106 Y CN2518106 Y CN 2518106Y CN 00215156 CN00215156 CN 00215156 CN 00215156 U CN00215156 U CN 00215156U CN 2518106 Y CN2518106 Y CN 2518106Y
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- antibody
- antigen
- utility
- model
- enzyme immunoassay
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Abstract
The utility model relates to a synchronous enzyme immunoassay device. The utility model is a polystyrene small rod composed of a handle, a small hook, a rod body and a ball part and ball part covered one or more than one stabilized antigen or antibody. The existing enzyme immunoassay device adopts a polystyrene microtiter plate as a solid-phase carrier, and can only detect one correspondent antibody or antigen or only can be used for the screening test of more than two antibody or antigen. By utilizing the utility model, the one step operation can also be realized without serum extraction or whole blood extraction, the confirming experiment of more than two detection items can be operated at the same time, and the screening test of more than two detection items can also be operated at the same time. The method of the operation is simple, quick and accurate, and the utility model is a technology worthy of developing.
Description
The utility model belongs to field of medical examination, is about a kind of improved enzyme immunoassay (EIA) device, particularly about a kind of synchronous enzyme immunoassay (EIA) device.
Existing enzyme immunoassay (EIA) device adopts polystyrene micropore plate as solid phase carrier usually, is about to antigen or antibody direct coated on polystyrene micropore plate, and back to be dried is standby as the enzyme immunoassay (EIA) device.Normally wrap by a kind of antigen (antibody) or two or more antigen (antibody) in a plate hole, only can detect a kind of corresponding antibody or antigen, or only can be used for the experiment of sieving of two or more antibody or antigen, originally it is any antibody or antigen that the experiment of sieving can not be proved conclusively simultaneously, still needs further confirmatory test.In addition, such device adopts usually and adds corresponding liquid enzyme label (enzyme-labelled antigen or antibody) in plate hole, the colour developing of washing back.Because plate hole is relatively-stationary, require each step operation in plate hole, to carry out, make operation steps loaded down with trivial details.Moreover, a kind of plate hole can only carry out a kind of confirmatory test, can not well carry out the combination experiment of two or more test items, promptly can not carry out two or more test items simultaneously confirms to test really, and each plate hole all need be carried out steps such as above-mentioned enzyme-added label, washing, colour developing, waste man power and material's (reagent), can not satisfy simple and rapid requirement.
Task of the present utility model just provides a kind of movably solid phase carrier to replace polystyrene micropore plate, as synchronous enzyme immunoassay (EIA) device.Be coated with one or more antigens (antibody) of stabilization on this solid phase carrier with physisorption or covalently bound mode, at room temperature long-term (1-2) is stable deposits.Adopt this utility model can carry out combination in any between two or more test items, can carry out two or more test items simultaneously and confirm really to test, also can carry out the experiment of sieving of two or more test items simultaneously.Method of operating is easy can hands-freely get serum or whole blood fast, can also carry out the single stage method operation.Preparation technology is simple, with low cost for this device.
Task of the present utility model is finished like this: there is one handle the upper end at the polystyrene spillikin, and a little hook is arranged on the barred body of handle bottom, and it is the bulb that the lower end of spillikin is spheroidal.With the bulb of polystyrene spillikin solid phase carrier as antigen or antibody, be coated with the two or more antigens (antibody) of stabilization on it with physisorption or covalently bound mode, at room temperature long-term (1-2) is stable deposits.Little hook on the barred body is used for fixing and hangs spillikin.The handle on this little hook top is used to take and indicates synchronous enzyme immunoassay (EIA) device.Because the enzyme immunoassay (EIA) device is movably synchronously, can make similar and different test item arbitrary combination, cooperates similar and different enzyme labeling thing again, thereby reaches the testing goal of carrying out two or more test items simultaneously.Utilize synchronous enzyme immunoassay (EIA) device, can directly the bulb be inserted in serum to be checked or the whole blood, and needn't extract sample, and measure when can realize the two or more test item of same sample.Also can carry out single stepping, mix with corresponding enzyme labeling thing after promptly extracting sample, then the bulb be inserted in this mixed liquor.Utilize little hook, synchronous enzyme immunoassay (EIA) device can be hung on the leaching device and wash, the synchronous enzyme immunoassay (EIA) device of similar and different test item can wash simultaneously.Easy and simple to handle, quick, sensitive, accurate, be applicable to two or more antigens or antibody in the synchronous working sample.
With regard to combination principle and accompanying drawing the utility model is described in further detail down.
Accompanying drawing is a structural representation of the present utility model.
The enzyme immunoassay (EIA) device is a kind of polystyrene spillikin [1] synchronously, be made up of handle [2], little hook [3], barred body [4] and bulb [5] from top to bottom, the lower end of spillikin is spheroidal, i.e. bulb [5], there is one handle [2] upper end of spillikin, and a little hook [3] is arranged on the barred body [4] of handle [2] bottom.With the bulb [5] of polystyrene spillikin [1] solid phase carrier as antigen or antibody, be coated with a kind of antigen (antibody) or more than one antigen (antibody) [6] of stabilization on it with physisorption or covalently bound mode, at room temperature long-term (1-2) is stable deposits.Little hook [3] on the barred body [4] is used for fixing and hangs spillikin.The handle [2] on this little hook [3] top is used to take and indicates synchronous enzyme immunoassay (EIA) device.Because the enzyme immunoassay (EIA) device is movably synchronously, can directly the bulb [5] of two or more synchronous enzyme immunoassay (EIA) devices be inserted in the serum to be checked simultaneously, [5] have been coated with one or more antigens (antibody) [6] of stabilization in the bulb, can with specific antibody or the antigen generation association reaction in the serum to be checked, and then be inserted in the mixed solution that contains two or more corresponding enzyme labeling things, can [5] the sandwich enzyme-linked immune response of corresponding sandwich take place in the bulb, by washing and colour developing, can reach the two or more antigens in the synchronous working sample or the purpose of antibody.At this, if adopting the bulb [5] of the polystyrene spillikin [1] of the two or more a kind of antigens (antibody) that are coated with stabilization inserts in the serum to be checked simultaneously, measure when can realize the two or more test item of same sample, and different test items can arbitrary combination, finishes the two or more test items of same sample synchronously and confirms really to test.If adopt the bulb [5] of the polystyrene spillikin [1] of the two or more two or more antigens (antibody) that are coated with stabilization to insert simultaneously in the serum to be checked, the experiment of sieving when can realize the two or more test item of same sample.Utilize synchronous enzyme immunoassay (EIA) device, needn't extract sample, directly will get final product in bulb [5] insertion serum to be checked or the whole blood.Also can carry out single stepping, mix with corresponding enzyme labeling thing after promptly extracting sample, then bulb [5] be inserted in the mixed liquor of sample and enzyme labeling thing.Cooperate cleansing solution and developer to wash again and develop the color.Utilize little hook [3], this utility model can be hung on the leaching device and wash, the synchronous enzyme immunoassay (EIA) device of similar and different test item can wash simultaneously.The bulb [5] of synchronous enzyme immunoassay (EIA) device can be placed colour developing liquid during colour developing, develop the color respectively (confirmatory test) or colour developing (experiment of sieving) simultaneously.Owing to when bulb [5] envelope antigen (antibody) of synchronous enzyme immunoassay (EIA) device, adopt the stabilization embodiment, can make this utility model at room temperature long-term (1-2) is stable and deposit.
During operation, get the two or more polystyrene spillikins [1] that are coated with the antigen (antibody) [6] of stabilization, its bulb [5] are inserted in the serum to be checked simultaneously, after 5-10 minute, put into the mixed liquor of two or more corresponding enzyme labeling things more simultaneously, after 5-10 minute, two or more polystyrene spillikins [1] are hung on simultaneously with little hook [3] carry out top-down washing on the leaching device, the bulb that to wash [5] place colour developing liquid colour developing (confirmatory test) respectively or place colour developing liquid colour developing (experiment of sieving) simultaneously then, it is positive to develop the color, and it is negative not develop the color.
The utility model is easy and simple to handle fast, and the result is easy to observe accurately and reliably.Its room temperature stability can be marked percolation with gold and compare favourably, be applicable to that sandwich with sandwich is that the enzyme immunoassay of principle is measured two or more antibody or antigen, can hands-freely get serum or whole blood, can also carry out the single stage method operation, can carry out two or more test items simultaneously and confirm really to test, also can carry out the experiment of sieving of two or more test items simultaneously.Preparation technology is simple for this utility model, and is with low cost.
Claims (2)
1, a kind of synchronous enzyme immunoassay (EIA) device, it is a kind of polystyrene spillikin [1], it is characterized in that from top to bottom being made up of handle [2], little hook [3], barred body [4] and bulb [5].
2, according to the synchronous enzyme immunoassay (EIA) device of claim 1 regulation, it is characterized in that the bulb [5] of polystyrene spillikin [1] is coated with the antigen or the antibody [6] of one or more stabilizations.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00215156 CN2518106Y (en) | 2000-06-13 | 2000-06-13 | Synchronous enzyme immunity analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00215156 CN2518106Y (en) | 2000-06-13 | 2000-06-13 | Synchronous enzyme immunity analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2518106Y true CN2518106Y (en) | 2002-10-23 |
Family
ID=33581152
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00215156 Expired - Fee Related CN2518106Y (en) | 2000-06-13 | 2000-06-13 | Synchronous enzyme immunity analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2518106Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106990233A (en) * | 2017-05-27 | 2017-07-28 | 李晓燕 | A kind of convex bubble plate of immune detection |
-
2000
- 2000-06-13 CN CN 00215156 patent/CN2518106Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106990233A (en) * | 2017-05-27 | 2017-07-28 | 李晓燕 | A kind of convex bubble plate of immune detection |
| CN106990233B (en) * | 2017-05-27 | 2019-05-07 | 于宁 | A kind of convex bubble plate of immune detection |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |