CN2454382Y - Rolling cultivation apparatus for cell engineering and tissue engineering - Google Patents
Rolling cultivation apparatus for cell engineering and tissue engineering Download PDFInfo
- Publication number
- CN2454382Y CN2454382Y CN 00252933 CN00252933U CN2454382Y CN 2454382 Y CN2454382 Y CN 2454382Y CN 00252933 CN00252933 CN 00252933 CN 00252933 U CN00252933 U CN 00252933U CN 2454382 Y CN2454382 Y CN 2454382Y
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- rolling
- culture
- incubator
- bed
- cell
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- 238000005096 rolling process Methods 0.000 title claims abstract description 75
- 238000012258 culturing Methods 0.000 claims description 27
- 238000010438 heat treatment Methods 0.000 claims description 6
- 229910001120 nichrome Inorganic materials 0.000 claims 1
- 238000004113 cell culture Methods 0.000 abstract description 11
- 230000010261 cell growth Effects 0.000 abstract description 6
- 230000001105 regulatory effect Effects 0.000 abstract description 3
- 238000009423 ventilation Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 24
- 230000009286 beneficial effect Effects 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 210000004408 hybridoma Anatomy 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000007769 metal material Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000011368 organic material Substances 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000008467 tissue growth Effects 0.000 description 2
- 238000005273 aeration Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
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Abstract
The utility model relates to a rolling culture apparatus for cell culture and tissue culture, which comprises a culture box, wherein a temperature regulating mechanism, an air flow regulating mechanism, a heater and a sensor are arranged in the culture box, and the heater and the sensor are connected with a temperature control regulator. The upper part of the culture box is provided with an air inlet, an air pipeline of which one end is inserted into the culture box is fixed on the air inlet, and the other end of the air pipeline is communicated with an air bottle through an air flow meter. The utility model also comprises a rolling bed, and more than two rolling culture bottles of which the both ends are provided with openings are arranged on the rolling bed. The lower parts of the rolling culture bottles are provided with a driving mechanism which is connected with a motor. Because the culture bottles with double openings can be used by the utility model, the ventilation is good. The rolling culture is carried out on the rolling bed, and thus, the utility model is suitable for the 3D growth of cells and tissue.
Description
The utility model relates to a kind of cell cultures and tissue culture device, especially for the rolling culture apparatus of cell engineering and organizational project.
The device that is used for cell cultures and tissue culture at present is a lot, except general CO2gas incubator, also has the culture apparatus of various special uses, as is used for the shaking table incubator of microorganism and culture plant cell, but yet there are no the rolling incubator.Existing rolling culture apparatus such as document 1 Freshhey R I., Cell culture of animal cells, A manual of basic technique, Second edition, 1987, Alan R.Liss, Inc., New York, pp309-317, what described is that bottle stand is cultivated in a kind of rolling, is not incubator.Though the culturing bottle of Gun Donging has not of the same racely simultaneously, all is the single port bottle, promptly the garden tubular rolls an only end opening of bottle.In order to be applicable to the dimensional culture of the cell in the organizational project, in recent years invented the rotation cell culture apparatus again, as document 2 Schwarz et al, Horizontally rotated cell culture system with a coaxial tubular oxygenator United States Patent No.5,026,650 is described.Above-mentioned two kinds of devices are commonly used and up-to-date culture apparatuses in cell engineering and the organizational project, but its limitation is arranged respectively, bottle stand is cultivated in present existing rolling can not be for being provided required temperature by cultured cells, environment such as gas, therefore it must with other culture apparatus, use jointly as common CO2gas incubator.Both in another incubator of packing into rolling bottle stand integral body.In addition, when carrying out cell cultures and tissue culture with this device, because the rolling culturing bottle of this device is a single port, single port rolling culturing bottle has following two shortcomings: the first, ventilate bad thus cell and tissue growth to be not so good as the twoport culturing bottle good.The second, because culturing bottle is the single port structure, it produces in imbalance in the axle rolling process and moves and vibration, and then influences the secretion of cell growth and cellular products, influences the 3 D stereo cultivation of cell especially.It equally is not culture apparatus that existing rotation cell culture apparatus is also cultivated bottle stand with rolling, and is swivel arrangement.Need to use jointly with other incubator, in other incubators of promptly packing into, in addition, because this apparatus structure complexity, the accuracy requirement height costs an arm and a leg in the manufacturing, complicated operation and difficult popularizing.
The purpose of this utility model is to overcome the shortcoming of above-mentioned culture apparatus, in order in cell cultures and tissue culture, to create the good aeration condition, with in order to guarantee that culturing bottle is keeping balance when cell cultures and the tissue culture in the axle rolling process, the 3 D stereo that is beneficial to cell and tissue is cultivated, and improves the output of cell stand density and emiocytosis thing; And in order further to improve the 3 D stereo incubation growth environment of cell and tissue, thereby provide a kind of cell engineering and organizational project rolling culture apparatus.
The purpose of this utility model is achieved in that
A kind of rolling culture apparatus that is used for cell engineering and organizational project that the utility model provides comprises incubator, control power supply, regulates and cultivates the temperature inside the box and CO
2The mechanism of airshed, control knob and indicating meter are installed on the control panel of incubator; It is interior or outer that the control power supply is contained in incubator; The temperature heater heating is installed in the incubator, is installed in to have on the sidewall of cultivating casing and carries out thermometric temperature sensor, well heater, temperature sensing are connected with the temperature control damper of outside; Cultivate casing top and be equipped with the gas inlet, fixing-gas pipeline on it, gas pipeline one end insert in the incubator, and the other end is communicated with gas cylinder by an adjustments of gas under meter.It is characterized in that: also comprise roll bed, the rolling culturing bottle of both ends open, driving mechanism; The described bed that rolls is installed in the bottom in the incubator, and the axis of rolling more than 2 is parallel to each other to be installed on the bed that rolls; The bed that rolls installs one down by controlling the driving mechanism that power supply, belt and motor are formed, and described driving mechanism comprises that the axis of rolling is connected with motor by belt; Motor is electrically connected with the control power supply; The cylindrical rolling culturing bottle of both ends open is placed on the axis of rolling.
The rolling culturing bottle is the cylindrical bottle of both ends open, roll bottle diameter and variable-length, and the rolling culturing bottle is placed on the axis of rolling.The rolling bottle rolls with stable rotating speed on the machinery rolling bed of controllable rotating speed, on the rolling bed a plurality of roller bearings is arranged, and a plurality of rolling culturing bottles can upward be done original position around the cylinder major axis in the bed that rolls and roll the while.The foraminous culturing bottle is opened at described two ends, and the hole is rotational symmetry or asymmetric opening on the cylinder both ends of the surface with the cylinder center; Wherein the edge of institute's perforate is an outward flanging mouth, spiral mouth or flat mouth.
At first open total power switch, utilize the temperature regulation button on the panel that temperature is transferred to needed temperature when cultivating, utilize CO
2Adjusting knob is with CO
2Amount is adjusted to required ratio (zooblast is generally 5%), makes stable of incubator lack a week, and whether the stability of observing temperature reaches standard, when guaranteeing temperature and CO
2Regulate in the time of all reaching standard, open chamber door, the twoport rolling bottle that inserts culture is placed on the rolling bed of culture apparatus, utilize the speed setting button on the panel to select required rolling speed.Shut in the case cultivation of rolling.And the attention monitor temperature, CO
2Amount and rotating speed.
A kind of rolling culture apparatus that is used for cell engineering and organizational project that provides of the present utility model has following advantage: (1) this device has overcome the unicity of existing incubator function, the rolling culture apparatus that provides of the present utility model had both had the 3 D stereo of cell and tissue and had cultivated required rolling cultivation function, had the function of general incubator again;
(2) since the use of this device be the twoport culturing bottle, ventilate, thereby be beneficial to cell and tissue growth; By adopting splendid attire culture in the twoport rolling culturing bottle, the culture in the culturing bottle is easy to keep balance in the axle rolling process on the bed that rolls, and be beneficial to the secretion of cell growth and cellular products again, and the 3 D stereo that is beneficial to cell and tissue is especially cultivated.The 3 D stereo that utilizes the rolling culture apparatus to carry out cell and tissue is cultivated and is significantly increased the hybridoma stand density, makes antibody production increase more than 30%; This rolling culture apparatus can replace knownly uniquely can carrying out three-dimensionally to external human cell, can break up, and has the mixed containers for culturing organisms of cultivating of different cells of tissue density;
(3) the utility model rolling culture apparatus is simple in structure, and manufacturing cost is low; And have advantages such as equipment easy handling.
Below in conjunction with accompanying drawing and example the utility model is described in detail:
Used twoport culturing bottle structural representation in Fig. 1 a-b the utility model rolling culture apparatus
Fig. 2 is the body structure synoptic diagram of the utility model rolling culture apparatus
Fig. 3 is the utility model rolling culture apparatus structural representation
Fig. 4 is the result schematic diagram of carrying out cell cultures in rolling culture apparatus of the present utility model
Drawing is described as follows:
1-incubator 2-adjustments of gas flow button 3-attemperation button
4-axis of rolling speed setting button 5-incubator external door 6-incubator inside door
The transparent glass window 8-rolling bed 9-culturing bottle of 7-external door
10-motor 11-temperature heating member (not shown)
12-illumination lamp 13-gas sensor 14-CO
2The gas inlet
The edge in 15-temperature sensor 16-hole is that the edge in flat 17-hole is the spiral mouth
18-axis of rolling 19-control power supply (not shown)
The rolling culture apparatus of present embodiment is assembled shown in Fig. 1-3, concrete structure is as follows: temperature heating rod 11 is installed in the heat insulating culture case 1 heats, the temperature sensor 15 that is installed on the sidewall of cultivating casing uses thermopair to measure, and heating rod 11, thermopair 15 are connected with the temperature control damper of outside.Cultivate casing top and be equipped with gas inlet 14, and fix a gas pipeline, gas pipeline one end inserts in the incubator, and an end is by adjustments of gas under meter and CO
2Gas cylinder is communicated with.On the sidewall of cultivation casing 1 illumination lamp 12 is housed; All control knobs and indicating meter are installed on the control panel of incubator 1 front (as shown in Figure 2), and be easy to operate.The bed 8 that perhaps will roll is put into the logical incubator of Pu, and control power supply 19 is put outside this incubator.The bed 8 that rolls is housed in heat insulating culture case 1 bottom, the bed that rolls is fixed a motor 10 8 times, on the bed 4 axis of rolling 18 is housed, and by belt motor 10 is connected with the axis of rolling 18, motor 10 is electrically connected with control power supply 19 output ports, and motor 10 velocities of rotation also can be regulated and control automatically.Drive the axis of rolling 18 by belt and roll when motor 10 rotates, 4 axis of rolling 18 carry out equidirectional rolling with identical speed simultaneously, and 3 culturing bottles 9 are placed between 4 axis of rolling 18, and it also carries out equidirectional rolling with identical speed.
The twoport culturing bottle 9 of present embodiment adopts nontoxic glass and organic materials and metallic substance to make, diameter is 50 millimeters, and length is 50 millimeters cylinder, is that respectively to open a diameter on the cylinder both ends of the surface be 10 millimeters hole 16 to rotational symmetry with the cylinder center, the edge in its hole is for flat mouthful, shown in Fig. 1 b.
Its culture apparatus is identical with embodiment 1, just the culturing bottle difference.The culturing bottle of present embodiment is that the edge by hole 17 shown in Fig. 1 a is the spiral shape of the mouth as one speaks.The twoport culturing bottle can adopt nontoxic glass and organic materials and metallic substance to make, it is 40 millimeters that present embodiment is made a diameter, length is 40 millimeters cylinder, on cylinder one end face be that to open a diameter be 10 millimeters hole to axle with the cylinder center, on the cylinder other end be that axle departs from 6 millimeters to open a diameter be 10 millimeters hole 17 with the cylinder center, the edge in its hole is the spiral mouth.
On the device of embodiment 1 and 2, carry out the cultivation of secrete monoclonal antibody hybridoma, and compare with a control group, its result as shown in Figure 3, among the figure-expression rotation group; Among the figure-the expression control group
Cultivation with secretion HCG monoclonal antibody hybridoma cell is an example.Used substratum is low sugar DMEM nutrient solution (contains 10% serum, contain 10,000 unit mycillins, pH value 7.2-7.4), 1.5 to 2.0 * 10
5/ ml viable cell is inoculated in the rolling bottle of 60ml, adds the DMEM nutrient solution to 10ml, puts on the bed that rolls and rolls.1.5 * 10
5/ ml viable cell is inoculated in 50ml, is static cultivation contrast in the T type bottle of 25cm2, adds the DMEM nutrient solution to 10ml.Sampling in per 24 hours from roll bottle, control bottle and T bottle is expected blue dyeing for 0.2%, determines viable count with Hematocyte Counter, and dead cell and total cell count are surveyed cell growth curve.The result as shown.Bottle and T bottle 4 days viable cell of cultured continuously and total cell count change curve in the nutrient solution that contains 10% serum respectively roll.
Claims (6)
1, a kind of rolling culture apparatus that is used for cell engineering and organizational project comprises incubator, control power supply, regulates the mechanism of cultivating the temperature inside the box and gas flow, and control knob and indicating meter are installed on the control panel of incubator; It is interior or outer that the control power supply is contained in incubator; The temperature heater heating is installed in the incubator, is installed in to have on the sidewall of cultivating casing and carries out thermometric temperature sensor, well heater, temperature sensing are connected with the temperature control damper of outside; Cultivate casing top and be equipped with the gas inlet, fix a gas pipeline on it, gas pipeline one end inserts in the incubator, and the other end is communicated with gas cylinder by an adjustments of gas under meter; It is characterized in that: also comprise roll bed, the rolling culturing bottle of both ends open, driving mechanism; The described bed that rolls is installed in the bottom in the incubator, and the axis of rolling more than 2 is parallel to each other to be installed on the bed that rolls; The bed that rolls installs one down by controlling the driving mechanism that power supply, belt and motor are formed, and described driving mechanism comprises that the axis of rolling is connected with motor by belt; Motor is electrically connected with the control power supply; The cylindrical rolling culturing bottle of both ends open is placed on the axis of rolling.
2. by the described a kind of rolling culture apparatus that is used for cell engineering and organizational project of claim 1, it is characterized in that: the culturing bottle that described two ends have mouth is to be rotational symmetry or asymmetric opening on the cylinder both ends of the surface with the cylinder center.
3. by claim 1,2 described a kind of rolling culture apparatuses that are used for cell engineering and organizational project, it is characterized in that: the edge of described culturing bottle both ends open is a flange mouth, spiral mouth or flat mouth.
4. by the described a kind of rolling culture apparatus that is used for cell engineering and organizational project of claim 1, it is characterized in that: described rolling bed is outer rim rolling bed.
5. by the described a kind of rolling culture apparatus that is used for cell engineering and organizational project of claim 1, it is characterized in that: also be included on the inner side-wall of cultivating casing the head light of using for culture plant cell is housed.
6. by the described a kind of rolling culture apparatus that is used for cell engineering and organizational project of claim 1, it is characterized in that: described temperature heater comprises heating rod, nichrome wire.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00252933 CN2454382Y (en) | 2000-11-15 | 2000-11-15 | Rolling cultivation apparatus for cell engineering and tissue engineering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00252933 CN2454382Y (en) | 2000-11-15 | 2000-11-15 | Rolling cultivation apparatus for cell engineering and tissue engineering |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2454382Y true CN2454382Y (en) | 2001-10-17 |
Family
ID=33610488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00252933 Expired - Fee Related CN2454382Y (en) | 2000-11-15 | 2000-11-15 | Rolling cultivation apparatus for cell engineering and tissue engineering |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2454382Y (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101058791B (en) * | 2007-05-14 | 2010-09-15 | 天津理工大学 | Method of culturing culture by roll load and bioreactor |
| CN101130752B (en) * | 2007-08-03 | 2011-05-04 | 天津理工大学 | Culture method of applying load evenly and bioreactor |
| CN101397539B (en) * | 2008-10-14 | 2011-10-05 | 中国人民解放军第三军医大学 | Force application apparatus simulating human physiological stress of tissue bionic culture for tissue engineering |
| CN101151361B (en) * | 2004-09-22 | 2012-02-01 | 米里坡有限公司 | Bioreactor assembly comprising at least one tray-like rocking platform |
| CN103614295A (en) * | 2013-11-27 | 2014-03-05 | 绍兴振德医用敷料有限公司 | Epidermal cell enriching instrument and dynamic enriching method thereof |
| CN103623001A (en) * | 2013-11-27 | 2014-03-12 | 绍兴振德医用敷料有限公司 | Enrichment and application of epidermis cells |
| WO2015078137A1 (en) * | 2013-11-27 | 2015-06-04 | 鲁建国 | Epidermal cell collecting instrument and dynamic collecting method thereof |
-
2000
- 2000-11-15 CN CN 00252933 patent/CN2454382Y/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101151361B (en) * | 2004-09-22 | 2012-02-01 | 米里坡有限公司 | Bioreactor assembly comprising at least one tray-like rocking platform |
| CN101058791B (en) * | 2007-05-14 | 2010-09-15 | 天津理工大学 | Method of culturing culture by roll load and bioreactor |
| CN101130752B (en) * | 2007-08-03 | 2011-05-04 | 天津理工大学 | Culture method of applying load evenly and bioreactor |
| CN101397539B (en) * | 2008-10-14 | 2011-10-05 | 中国人民解放军第三军医大学 | Force application apparatus simulating human physiological stress of tissue bionic culture for tissue engineering |
| CN103614295A (en) * | 2013-11-27 | 2014-03-05 | 绍兴振德医用敷料有限公司 | Epidermal cell enriching instrument and dynamic enriching method thereof |
| CN103623001A (en) * | 2013-11-27 | 2014-03-12 | 绍兴振德医用敷料有限公司 | Enrichment and application of epidermis cells |
| CN103614295B (en) * | 2013-11-27 | 2014-07-16 | 绍兴振德医用敷料有限公司 | Epidermal cell enriching instrument and dynamic enriching method thereof |
| WO2015078137A1 (en) * | 2013-11-27 | 2015-06-04 | 鲁建国 | Epidermal cell collecting instrument and dynamic collecting method thereof |
| CN103623001B (en) * | 2013-11-27 | 2016-06-29 | 绍兴振德医用敷料有限公司 | The enrichment application of epidermis cell |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |