CN2421652Y - Multinomial synchronous enzyme immune percolator - Google Patents
Multinomial synchronous enzyme immune percolator Download PDFInfo
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- CN2421652Y CN2421652Y CN 00214037 CN00214037U CN2421652Y CN 2421652 Y CN2421652 Y CN 2421652Y CN 00214037 CN00214037 CN 00214037 CN 00214037 U CN00214037 U CN 00214037U CN 2421652 Y CN2421652 Y CN 2421652Y
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Abstract
The utility model relates to a multinomial synchronous enzyme immune percolator, which is suitable for synchronously detecting more than two antibodies or antigens by utilizing an enzyme immune percolating method which adopts the principle of sandwich cores. The utility model is a multilayer box type device which is composed of a filtering extension layer, an enzyme labeling object layer, a percolation layer and a waste liquid absorbing layer. The existing enzyme immune percolator usually spots directly the antigen or the antibody on a nitrocellulose membrane; then the nitrocellulose membrane is used as a percolation membrane after dried, and thus one percolation membrane can only detect one original antibody or antigen. When the utility model is used, more than two items of detection results can be obtained by adding serum samples at one time and washing coloration. Therefore, the utility model is technology which is worthy of development, and besides, the utility model has the advantages of convenient operation and high accuracy.
Description
The utility model belongs to field of medical examination, is about a kind of improved enzyme immunity percolation device, particularly about a kind of multinomial synchronous enzyme immunity percolation device.
Existing enzyme immunity percolation device adopts usually with antigen or the direct point sample of antibody on cellulose nitrate (NC) film, and back to be dried is standby as diafiltration membrane.Normally bag only can be detected a kind of corresponding antibody or antigen by a kind of antigen or antibody on a kind of diafiltration membrane of percolating device.In addition, these class methods adopt liquid enzyme-labelled antigen or antibody as the enzyme labeling thing usually, have so not only influenced the stability and the storage life of this method and apparatus, and operation steps is increased.Can not well reach quick, accurate, fast, the simple requirement of current immunologic assay.
Task of the present utility model just provides a kind of solid phase specific antigen or the diafiltration membrane of antibody and multinomial synchronous enzyme immunity percolation device of two or more immobilized enzyme labels that contains two or more stabilizations, at room temperature long-term (1-2) is stable deposits, have easy method of operating and sensitive, advantage accurately, once add and only need wash colour developing behind the blood serum sample and can obtain two above testing results simultaneously, and preparation technology is simple, and is with low cost.
Task of the present utility model is finished like this: comprise from top to bottom in box body and filter extension layer, enzyme labeling thing layer, filter layer and waste liquid absorption layer.Each layer, wherein filters extension layer, enzyme labeling thing layer, waste liquid absorption layer and all adopts qualitative or quantitative filter paper as holder with filter paper and cellulose membrane, and filter layer adopts nitrocellulose filter.The layer with layer between according to above-mentioned top-down order overlapping and close the contact.Adopt the specific antigen of two or more stabilizations or the antibody-solutions zones of different of point sample on nitrocellulose filter respectively, it is air-dry standby to be placed on room temperature through the bovine serum albumin solution sealing then.The nitrocellulose filter of point sample is cut into prescribed level according to selected zone promptly become filter layer.The same zones of different of enzyme labeling thing solution difference point sample on filter paper that adopts two or more stabilizations, the mixed solution point sample of two or more enzyme labeling things that also can adopt stabilization places room temperature air-dry and cut into prescribed level and promptly become enzyme labeling thing layer on filter paper then.The making vacuum that not only can save nitrocellulose filter is drained process like this, and in dry run, because the enforcement of static stabilization, can make the activity of antigen or antibody and enzyme labeling thing keep stable, activity that the more important thing is the two or more antigens that can make on nitrocellulose filter or the filter paper or antibody and enzyme labeling thing keeps stable in drying regime, can stablize 1-2 under room temperature.Cooperate cleansing solution and reagent composition simultaneously.Adopt this multinomial synchronous enzyme immunity percolation device only to need an application of sample can wash colour developing, omitted the step that adds two or more liquid enzyme labels, in easy quick synchronous operation, reach the purpose that primary sample can obtain two above testing results.This multinomial synchronous enzyme immunity percolation device can at room temperature be stablized and deposits, and its detection method is easy, quick, sensitive, accurate, is applicable to fast two or more antigens or antibody in the working sample synchronously.
Just in conjunction with the accompanying drawings the utility model is described in further detail down.
Accompanying drawing is a structural representation of the present utility model.
The multilayer box structure that multinomial synchronous enzyme immunity percolation device is made up of multilayer filter paper and cellulose membrane, extension layer [1] filters in the superiors, is qualitative or quantitative filter paper, is used to expand serum and filtration.The second layer is an enzyme labeling thing layer [2], is qualitative or quantitative filter paper, and the two or more immobilized enzyme labels [6] of stabilization are arranged on the filter paper, lays respectively at a zones of different on the filter paper, is round point shape or other shapes.The 3rd layer is filter layer [3], be nitrocellulose filter, the solid phase specific antigen or the antibody [7] that contain two or more stabilizations on the film, lay respectively at a zones of different on the nitrocellulose filter, be round point shape or other shapes, the nitrocellulose filter of point sample puts that room temperature is air-dry to get final product after bovine serum albumin solution sealing.The 4th layer is waste liquid absorption layer [4], mainly contains multi-layer absorbent filter paper or other absorbent materials [8] and forms.More than each layer be circle, square or other shapes that heartcut becomes prescribed level with the point sample zone.Layer with layer between according to the point of sample of above-mentioned top-down order and same test event principle one to one, overlapped and close contact, then each layer put into the little box body [9] of respective shapes and size, the top of capsule respectively has a zone to be sample application zone [5] directly over each point of sample, there is a joint portion [10] at the middle part of capsule between enzyme labeling thing layer [2] and cellulose membrane filter layer [3], there is opening device [11] at this position, can open, can and filter extension layer [1] and open together enzyme labeling thing layer [2] during unlatching, all expose filter layer [3], be beneficial to observations, observe the colour developing situation of spot on the nitrocellulose filter.This joint portion [10] has sign on every side, is beneficial to the difference of projects.
The utility model is easy and simple to handle fast, and the result is easy to observe accurately and reliably.Its room temperature stability can be marked percolation with gold and compare favourably, be applicable to that sandwich with sandwich is that the enzyme immunity percolation method of principle is measured two or more antibody or antigen, once add and only need wash colour developing behind the blood serum sample and can obtain two above testing results simultaneously, and make easily, and be fit to produce in batches.
During operation, get an amount of serum to be checked and splash into respectively in the sample application zone [5], treat diafiltration, get an amount of cleansing solution again and splash into respectively in each sample application zone [5], treat diafiltration, splash into an amount of cleansing solution after 5-10 minute more respectively, treat diafiltration, give a baby a bath on the third day after its birth altogether time, in each sample application zone [5], splash into developer respectively subsequently, treat diafiltration, add cleansing solution at last again, treat diafiltration, give a baby a bath on the third day after its birth altogether time, open joint portion [10] then and make filter layer [3] all expose observations, the spot colour developing is positive, and spot is colourless negative.(also can after opening joint portion [10], splash into developer, cleansing solution and observations more respectively).
Claims (4)
1, a kind of multinomial synchronous enzyme immunity percolation device, it is characterized in that being added with one deck enzyme labeling thing layer [2] between box body [9] inner filtration extension layer [1] and filter layer [3] by filtering the multilayer cassette arrangement that extension layer [1], filter layer [3] and waste liquid absorption layer [4] are formed.
2,, it is characterized in that containing in the enzyme labeling thing layer [2] the immobilized enzyme label [6] of two or more stabilizations according to the multinomial synchronous enzyme immunity percolation device of claim 1 regulation.
3,, it is characterized in that containing in the filter layer [3] the solid phase specific antigen or the antibody [7] of two or more stabilizations according to the multinomial synchronous enzyme immunity percolation device of claim 1 regulation.
4, according to the multinomial synchronous enzyme immunity percolation device of claim 1 regulation, it is characterized in that there is a joint portion [10] at the middle part in box body [9] between enzyme labeling thing layer [2] and filter layer [3], there is an opening device [11] at this position.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN 00214037 CN2421652Y (en) | 2000-04-03 | 2000-04-03 | Multinomial synchronous enzyme immune percolator |
Applications Claiming Priority (1)
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CN 00214037 CN2421652Y (en) | 2000-04-03 | 2000-04-03 | Multinomial synchronous enzyme immune percolator |
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CN2421652Y true CN2421652Y (en) | 2001-02-28 |
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CN 00214037 Expired - Fee Related CN2421652Y (en) | 2000-04-03 | 2000-04-03 | Multinomial synchronous enzyme immune percolator |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104569432A (en) * | 2015-01-06 | 2015-04-29 | 通标标准技术服务有限公司 | Method and device for detecting transgenic ingredients in food |
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2000
- 2000-04-03 CN CN 00214037 patent/CN2421652Y/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104569432A (en) * | 2015-01-06 | 2015-04-29 | 通标标准技术服务有限公司 | Method and device for detecting transgenic ingredients in food |
CN104569432B (en) * | 2015-01-06 | 2016-10-05 | 通标标准技术服务有限公司 | The detection method of a kind of transgene component in food and device |
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GR01 | Patent grant | ||
C19 | Lapse of patent right due to non-payment of the annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |