CN2394719Y - Static sorting device for plant diploid pollen particles - Google Patents

Static sorting device for plant diploid pollen particles Download PDF

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Publication number
CN2394719Y
CN2394719Y CN 99221312 CN99221312U CN2394719Y CN 2394719 Y CN2394719 Y CN 2394719Y CN 99221312 CN99221312 CN 99221312 CN 99221312 U CN99221312 U CN 99221312U CN 2394719 Y CN2394719 Y CN 2394719Y
Authority
CN
China
Prior art keywords
pollen
pole plate
plant
pollen particles
pollen grain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 99221312
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Chinese (zh)
Inventor
郭清南
张路军
李法德
张晓辉
杨传友
侯存良
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Agricultural University
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Shandong Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong Agricultural University filed Critical Shandong Agricultural University
Priority to CN 99221312 priority Critical patent/CN2394719Y/en
Application granted granted Critical
Publication of CN2394719Y publication Critical patent/CN2394719Y/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The utility model relates to a pollen particle sorting device, which is used for plant polyploid breeding. The utility model is mainly composed of a metal screening pipe which is a feeding mouth, a pole plate, a high-voltage generator, an adjusting knob, a pole plate frame, a slide block, a slide rail, a clamping spring, an upper cover, a box body, etc. The utility model can be used for sorting diploid pollen particles form the pollen particle mixture of the diploid pollen particles and haploid pollen particles, the frequency of the diploid pollen particles is improved, and finally, the diploid pollen particles can be conveniently used on plant polyploid breeding.

Description

Plant dliploid pollen grain electrostatic separation apparatus
The utility model belongs to the pollen grain sorting unit for plant polyploid breeding service.
In the existing plant polyploid breeding process, the sorting of 2n pollen grain (dliploid pollen grain) all is to adopt the wet separation method, operates pretty troublesomely, and the efficiency of separation is low, and be not suitable for and do not allow the plant variety of soaking before the pollen pollination, limited the progress of plant polyploid breeding.
The purpose of this utility model provides a kind of plant dliploid pollen grain electrostatic separation apparatus, adopt dry sorting method, the 2n pollen grain is sorted out from the mixture of n pollen grain (monoploid pollen grain) and 2n pollen grain, improve the frequency of 2n pollen grain, thereby improve the probability that utilizes crossbreeding to produce polyploid.
The utility model links to each other positive and negative pole plate respectively with positive and negative the two poles of the earth of high-voltage DC power supply, produce strong electrostatic field between pole plate.After 2n and n pollen grain enter strong electrostatic field,, thereby drop on the different parts of pole plate and separated opening, can greatly improve breeder's operating efficiency along different orbiting motions.
The utility model can improve the frequency of 2n pollen grain greatly, can with the frequency of 2n pollen grain by 1% under the nature about, bring up to more than 20%.This will very conveniently be used for crossbreeding with 2n pollen, will improve the probability that crossbreeding produces polyploid greatly, be the application developing bright prospect of 2n pollen on polyploid breeding, and the economic benefit of being brought is very huge thus.Polyploid breeding can be bred the new varieties of many good polyploids simultaneously, will improve the people's material well-being greatly, will bring very big social benefit thus.
Description of drawings:
Fig. 1 is a dliploid pollen grain electrostatic separation apparatus profile.
1 is the metallic shield pipe of double pollen feeding mouth among the figure, the 2nd, and loam cake, the 3rd, pole plate, the 4th, slide block, the 5th, adjusting knob, the 6th, pole plate frame, the 7th, fastening spring, the 8th, slide rail, the 9th, casing, the 10th, high pressure generator, the 11st, power-line terminal.
Fig. 2 is a dliploid pollen grain electrostatic separation apparatus front view.
Fig. 3 is a dliploid pollen grain electrostatic separation apparatus vertical view.
The utility model most preferred embodiment:
The metallic shield pipe (1) of double pollen feeding mouth is fixed on the loam cake (2).Loam cake (2) fastening forms the still air space that is not subjected to external interference on casing (9).Pole plate (3) is clamped on the pole plate frame (6) by fastening spring (7), and pole plate frame (6) is connected on the sliding slider (4).Rotate adjusting knob (5) and can spur slide block (4), thereby change the distance between two-plate along slide rail (8) slip.Pole plate (3) is connected with lead with high-voltage D.C. generator (10), thereby forms strong UNIFORM ELECTROSTATIC FIELD between positive/negative plate.
The metallic shield pipe (1) of double pollen feeding mouth adopts conduction good metal material to be made, the cross section is the rectangle that is about 3 * 10cm, highly be about 20-30cm, pole plate makes the rectangle that is shaped as 10 * 20cm of pole plate by very high metal of fineness or transparent non-metal board.Loam cake (2), casing (9), slide block (4), adjusting knob (5) all are to be made by insulation good insulation performance material, jeopardize operator's safety in order to avoid high-tension electricity leaks outside.For the ease of observing the sorting situation of pollen grain, make with transparent insulating materials on the two sides, front and back of loam cake (2).
During use, the end opening of the metallic shield pipe (1) of double pollen feeding mouth is inserted into the inside of positive and negative pole plate, got rid of the interference of inhomogeneous field to the pollen sorting, formed static air space again, make 2n and n pollen grain have different sinking speed in the process of free settling in the still air in this section, obtained initial gross separation.2n and n pollen grain with different sinking speed, drop in the uniform electric field of pole plate (3) after, obtain negative electrical charge, be subjected to the effect of electric field force, fall within the different parts of positive plate with different side velocities and sinking speed.The n pollen grain falls within the top of pole plate, and the 2n pollen grain falls within the below of pole plate.Thereby realized purpose that the 2n pollen grain is sorted out.
The utility model is in sorting pollen process, and pollen does not contact with liquid fully, the below is contained the many pollen grains of 2n pollen grain from the positive plate and sweeps, and can be directly used in pollination.Can improve the probability that crossbreeding produces polyploid so greatly, avoid of the infringement of wet separation method liquid simultaneously the pollen vigor.While the utility model, spacing that can be by adjusting pole plate (3) and the voltage by change high pressure generator (10) change the voltage between pole plate, adjust the electric-field intensity between pole plate (3), thereby guarantee to make pollen to be in the electric-field intensity sorting of high germination rate.Experiment of the present utility model has proved the pollen that adopts after the suitable electric-field intensity sorting, can make germination percentage obtain by a relatively large margin raising.
Use of the present utility model proves, the device of this sorting pollen is simple, and is easy to use, not only pollen do not caused damage, and can improve the germination percentage of pollen, the frequency ratio of 2n pollen grain can both be greatly enhanced with other any method sortings.Therefore be the plant polyploid breeding, a kind of effective operation tool is provided, be with a wide range of applications.

Claims (3)

1. plant dliploid pollen grain electrostatic separation apparatus, mainly form by shielded-plate tube, high pressure generator, adjusting knob, pole plate, slide rail, fastening spring, casing shell and box cover, it is characterized in that shielded-plate tube is installed in box cover central authorities, pole plate frame peace is solid on the slide block of slide rail the inner, the slide rail outer end is fixed on the casing shell, and adjusting knob is installed.
2. plant dliploid pollen grain electrostatic separation apparatus according to claim 1 is characterized in that fastening spring is installed on the pole plate frame, and pole plate is clamped on the spring clip.
3. plant dliploid pollen grain electrostatic separation apparatus according to claim 1 is characterized in that pole plate made by glass plate, is connected with the high-voltage D.C. generator lead, and the high-tension terminal peace is solid on the casing shell.
CN 99221312 1999-06-21 1999-06-21 Static sorting device for plant diploid pollen particles Expired - Fee Related CN2394719Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 99221312 CN2394719Y (en) 1999-06-21 1999-06-21 Static sorting device for plant diploid pollen particles

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 99221312 CN2394719Y (en) 1999-06-21 1999-06-21 Static sorting device for plant diploid pollen particles

Publications (1)

Publication Number Publication Date
CN2394719Y true CN2394719Y (en) 2000-09-06

Family

ID=34011897

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 99221312 Expired - Fee Related CN2394719Y (en) 1999-06-21 1999-06-21 Static sorting device for plant diploid pollen particles

Country Status (1)

Country Link
CN (1) CN2394719Y (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106993447A (en) * 2017-04-05 2017-08-01 重庆光电信息研究院有限公司 A kind of plant pollen processing unit
CN107457082A (en) * 2017-07-03 2017-12-12 中国矿业大学 The adjustable friction electrical selection sorting unit of multisection type high voltage electric field and method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106993447A (en) * 2017-04-05 2017-08-01 重庆光电信息研究院有限公司 A kind of plant pollen processing unit
CN107457082A (en) * 2017-07-03 2017-12-12 中国矿业大学 The adjustable friction electrical selection sorting unit of multisection type high voltage electric field and method

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C14 Grant of patent or utility model
GR01 Patent grant
C19 Lapse of patent right due to non-payment of the annual fee
CF01 Termination of patent right due to non-payment of annual fee