CN2335972Y - Water-jackted CO2 cell culture box - Google Patents
Water-jackted CO2 cell culture box Download PDFInfo
- Publication number
- CN2335972Y CN2335972Y CN 97239096 CN97239096U CN2335972Y CN 2335972 Y CN2335972 Y CN 2335972Y CN 97239096 CN97239096 CN 97239096 CN 97239096 U CN97239096 U CN 97239096U CN 2335972 Y CN2335972 Y CN 2335972Y
- Authority
- CN
- China
- Prior art keywords
- water
- temperature
- ultrasonic transducer
- wind wheel
- water jacket
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model relates to a novel water-jackted CO< 2 > cell incubator. The top of the incubator body is made into a stepped water jacket; a motor is installed at the outside of the water jacket; a motor shaft and conducting wires of a sensor are connected with a wind wheel and a sensor which are arranged in the cell incubator. The vicinity of the wind wheel is provided with an air heater whose starting is controlled by a door switch; the sensor adopts an ultrasonic transducer and is connected with a control circuit; the output end of the control circuit is connected with a display panel meter and a gas valve switch. The utility model is not affected by environment temperature, having the advantages of easy adjustment, simple circuit, high detection control precision, long service life and good performance.
Description
The utility model relates to a kind of plant and instrument in medical science, the biotechnology, particularly relates to the thermostat container of doing animal cell culture in a kind of medical science, the bioengineering field.
Existing medical science, animal cell culture case in the biotechnology, volume is generally at the 50-200 liter, uniform and stable for guaranteeing culturing room's temperature, usually culturing room's five faces are water jackets that the stainless steel interlayer forms, the front is to seal the glass door that is used to observe, heating unit is in the bottom, cause the constant temperature of culturing room by the convection circulation transmission of water, for guaranteeing not dehydration of top, last thickness of interlayer is about 50-60mm, and for placing puddle blower fan and transmitter, design has near coal-mine formula structure at last interlayer middle part, near coal-mine place does not have the interlayer of water, this near coal-mine formula structure has reduced the room temp control accuracy, and when envrionment temperature was low, near the room temp the near coal-mine place was lower, form low temperature and pressure, when envrionment temperature is higher,, the water temperature of layer interlayer is raise gradually because of electric motor long-term operation heating, high-temperature water constantly accumulates in top, makes culturing room's upper temp too high.Because place electric motor, transmitter, near coal-mine size can not be done too for a short time, therefore when culturing room hour, influence greatlyyer, in the room that does not have conditioning unit, summer, equipment can not use usually.Simultaneously, for the external conditions of zooblast growth and breeding is provided, culturing room will guarantee certain sour environment, promptly guarantees certain CO
2Gas concentration generally about 5%, and detects CO at present
2The transmitter of gas concentration divides two kinds of heat-conducted and infrared rays, has debug difficulties, easily is corroded, complex structure, shortcoming such as work-ing life is short, influenced by ambient temperature, the detection control accuracy is low.
The purpose of the utility model aims to provide and a kind ofly is not subjected to envrionment temperature and the motor feels hot that influence, the open the door compensation of heating rapidly realizes constant temperature and adopts CO in the new sensor detecting controlling box
2Concentration, adjust easily, circuit is simple, long service life, novel water-jacket typ CO that control accuracy is high
2Cell culture incubator.
Technical solution of the present utility model is: this novel water-jacket typ CO
2Five of its casings of cell culture incubator are the intercalated water sleeve structure, the front is sealable glass door, heating unit is installed in the bottom, the utility model changes the casing top into step-like water jacket, electric motor is contained in the water jacket outside, motor reel and sensor conductor respectively with case in wind wheel be connected with transmitter, near the wind wheel airheater is housed in the case, start by door switch control, transmitter adopts ultrasonic transducer, circuit structure is: the oscillation signal of oscillatory circuit output is divided into two-way: the band time-delay late of one road do, by the ultrasonic transducer emission, process contains CO after amplifying on another road
2Accepted to amplify shaping by another part ultrasonic transducer behind the air of gas, the two-way oscillation signal converges the back stack, the variation reflection CO of dutycycle
2The variation of gas concentration, output connects panel table and air valve switch respectively.
The utility model is compared with prior art, because the casing top is step-like water jacket, in upper strata water jacket rear design is the step-like of high 100 millimeter, rest part has only 10 millimeter height, Bao thickness helps electric motor and is contained in water jacket top so especially, motor reel can be by wind wheel puddle in the hollow bolt connecting box, and this step-like jacket structure for water can remain on the long period use and not dehydration, make the casing upper strata be in the encirclement of water jacket samdwich all the time, when envrionment temperature is low, cold zone can not appear in case, when envrionment temperature is higher, though water is subjected to heating of electric motor overheating, but high-temperature water also can rise to the top of step-like water jacket rapidly, can not influence the temperature inside the box and form the high-temperature zone, thereby the temperature inside the box is even, good constant-temperature effect, precision improves greatly, when open when getting thing at the incubator Qianmen, heat scatters and disappears, temperature can descend, but well heater is controlled by door switch near the wind wheel of the utility model design, start the higher and heat of Heating temperature and taken away puddle by wind wheel, when making temperature in the culturing room rise to rapidly near design temperature, by temperature sensor control airheater is cut off the power supply, temperature persistance and precision are further improved, help the growth of cell, the utility model adopts ultrasonic transducer to detect control CO
2Gas concentration has also overcome existing heat-conducted and debug difficulties that two kinds of transmitters of infrared rays exist, easily has been corroded, complex structure, fragile, influenced by ambient temperature, shortcoming such as precision is low.
The utility model is reasonable in design, simple in structure, debugging easily, not influenced by ambient temperature, precision is high, technical feature is advanced, be difficult for the burn into long service life, for the cultivation of field zooblasts such as China's medical science, biotechnology provides a kind of good equipment.
Fig. 1 is the utility model structural representation;
Fig. 2 is the utility model CO
2Concentration ultrasound examination pilot circuit figure.
Below in conjunction with accompanying drawing the utility model is described in further detail:
Referring to Fig. 1, evenly stablize for guaranteeing the incubator room temp, it is sealable glass door (1) that incubator all is designed to the front, all the other five faces of casing (2) are stainless steel water nested structures of interlayer, heating unit (3) is installed in the bottom, convection circulation transmission by water causes culturing room's constant temperature, the utility model is made step-like water jacket (4) with casing (2) top, intercalated water sleeve is thin especially on the front portion, thickness reduces to 10 millimeter, therefore can make electric motor (6) be contained in the top of water jacket (4), motor reel connects wind wheel (5) puddle by hollow bolt (7), and sensor conductor also is connected with case inner sensor (8) by hollow bolt (7).For making not dehydration of interlayer, the step-like structure that high 100 millimeter are arranged in water jacket upper strata rear design, both made the upper strata rest part have only 10 millimeters not dehydrations with using also can be longer the time, and made the upper strata be in water jacket samdwich all the time and surround, and cold zone can not occur.When envrionment temperature was higher, water was subjected to heating of electric motor overheating, and high-temperature water also can rise to the top of step-like water jacket rapidly, and does not influence indoor temperature, and step-like structure has also made full use of the space, and water storage is more, if having a power failure, soaking time is longer.The hollow bolt that motor shaft is passed through is fabricated, and not welding in the middle of steel plate makes the water jacket upper strata indeformable on the technology, and this structure more helps the less incubator of volume.
After homo(io)thermism in the water jacket, culturing room's temperature is also with regard to the temperature unanimity of fundamental sum water.When opening the front glass door and get thing, indoor warm air leaks, temperature can descend, because culturing room's temperature of water temperature and requirement almost is consistent, therefore it is long will leaning on the transmission of water temperature to make culturing room's thermal recovery time, general decline 5 ℃ recovers to want about 15 minutes fully, and pair cell is cultivated like this certain influence.The utility model is equipped with airheater near the puddle wind wheel in culturing room, airheater (9) is made up of thin resistance wire, start by glass door switch (10), after opening the door, touch door switch, airheater just energising is heated, heat is taken away by wind wheel, because of the heater table surface temperature higher, the cultivation room temp is risen rapidly, during near design temperature, by the outage of transmitter control air well heater, because of airheater (9) is made up of the very little resistance wire of quality, thermal content is less, and outage back waste heat can not make culturing room's temperature overshot.Do not have the startup of door switch 10, airheater can not heated, and culturing room keeps original stability state, and the temperature that the back culturing room of opening the door is descended is recovered rapidly, helps the growth of cell.
Utilize ultrasonic transducer to detect control CO
2The gas concentration principle is: the aerial velocity of propagation V=340M/S of ultrasonic wave, and at CO
2Velocity of propagation V=258M/S in the gas has certain density CO in air
2Behind the gas, ultrasonic propagation velocity will descend, and ultrasonic transducer utilizes this phenomenon to detect airborne CO
2Concentration.
Circuit theory is referring to shown in Figure 2:
By U
1(4069) two not gates of A, B are formed the RC vibrator, and frequency is adjusted to 80KH
2, after the C not gate shaping of footpath, by U
3: single d type flip flop (4013) frequency division of B is 40KH
2After, being divided into two-way, the first via is done to promote to amplify by D, E, F not gate, by Q
1, Q
2Push off power magnification, promote ultrasonic transducer work, by ultrasonic transducer S
1The ultrasonic wave that transmits is by ultrasonic transducer S
2Receive footpath U
2(4069) A, B, C, D do three grades of amplifications, do the shaping upset through not gate E again and are passed to single d type flip flop; Another road is through U
4(4528) two monostable circuits of A, B are done twice sluggish time-delay, also are passed to single d type flip flop, adjust RV
2, making output voltage is zero.When adding certain C O
2Behind the gas, cause S
2The signal phase reach that comes, two-way signal are added to behind single d type flip flop, change in duty cycle, and output voltage no longer is zero, reflection CO
2The voltage signal of gas concentration is through R
9, C
6, R
10The T L-type filter that constitutes forms direct current stably, is input to U
6: B (358) makes integration and amplifies.Output signal one road is to panel table MAD demonstration, one road to U
7: A and setting signal relatively trigger zero-crossing comparator work, trigger U
5(3081) photoelectricity coupling silicon controlled rectifier work is with one control air valve switch Q
3
Claims (2)
1, a kind of novel water-jacket typ CO
2Cell culture incubator, have casing (2) and glass door (1), it is characterized in that casing (2) top is step-like water jacket (4), electric motor (6) is contained in water jacket (4) outside, motor reel and sensor conductor respectively with casing (2) in wind wheel (5) be connected with transmitter (8), near the wind wheel (5) airheater (9) is housed in the case, starts by door switch (10) control.
2, novel water-jacket typ CO according to claim 1
2Cell culture incubator is characterized in that transmitter (8) adopts ultrasonic transducer (S
1, S
2), circuit structure is: oscillatory circuit (U
1) oscillation signal of output is divided into two-way, the one tunnel makes sluggish time-delay (U
4), another road is through amplifying (U
1: D, E, F, Q
1, Q
2) back by ultrasonic transducer (S
1) emission, through containing CO
2Behind the air of gas by another part ultrasonic transducer (S
2) receive, amplify shaping (U
2: A, B, C, D, E), the two-way oscillation signal converges back stack (U
3: A), the variation of dutycycle reflection CO
2The variation of gas concentration, output connects panel table (U respectively
6, MAD) and air valve switch (U
7, Q
3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97239096 CN2335972Y (en) | 1997-10-30 | 1997-10-30 | Water-jackted CO2 cell culture box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 97239096 CN2335972Y (en) | 1997-10-30 | 1997-10-30 | Water-jackted CO2 cell culture box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2335972Y true CN2335972Y (en) | 1999-09-01 |
Family
ID=33949210
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 97239096 Expired - Fee Related CN2335972Y (en) | 1997-10-30 | 1997-10-30 | Water-jackted CO2 cell culture box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2335972Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102978112A (en) * | 2012-11-28 | 2013-03-20 | 中国人民解放军海军医学研究所 | High-pressure cell incubator, temperature control method and pressure control method thereof |
| CN107478718A (en) * | 2017-08-09 | 2017-12-15 | 浙江省计量科学研究院 | A kind of automatic sterile detection means and method |
| CN109505472A (en) * | 2019-01-07 | 2019-03-22 | 广东东箭汽车科技股份有限公司 | The electronic lock of automobile side-door and automobile |
| CN112980687A (en) * | 2021-05-19 | 2021-06-18 | 广州维柏生物科技有限公司 | Constant temperature and humidity cell culture box |
-
1997
- 1997-10-30 CN CN 97239096 patent/CN2335972Y/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102978112A (en) * | 2012-11-28 | 2013-03-20 | 中国人民解放军海军医学研究所 | High-pressure cell incubator, temperature control method and pressure control method thereof |
| CN102978112B (en) * | 2012-11-28 | 2014-10-08 | 中国人民解放军海军医学研究所 | High-pressure cell incubator, temperature control method and pressure control method thereof |
| CN107478718A (en) * | 2017-08-09 | 2017-12-15 | 浙江省计量科学研究院 | A kind of automatic sterile detection means and method |
| CN109505472A (en) * | 2019-01-07 | 2019-03-22 | 广东东箭汽车科技股份有限公司 | The electronic lock of automobile side-door and automobile |
| CN112980687A (en) * | 2021-05-19 | 2021-06-18 | 广州维柏生物科技有限公司 | Constant temperature and humidity cell culture box |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |