CN112980687A - Constant temperature and humidity cell culture box - Google Patents
Constant temperature and humidity cell culture box Download PDFInfo
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- CN112980687A CN112980687A CN202110543812.0A CN202110543812A CN112980687A CN 112980687 A CN112980687 A CN 112980687A CN 202110543812 A CN202110543812 A CN 202110543812A CN 112980687 A CN112980687 A CN 112980687A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
The invention discloses a constant temperature and humidity cell culture box. The incubator comprises a box body, a box door, chambers, a water heating mechanism, a humidifying disc, a supporting rod, a humidity maintaining mechanism, a CO2 generator, a first exhaust pipe and a CO2 concentration maintaining mechanism, wherein one side of the box body is movably connected with the box door, three chambers with equal volumes are arranged inside the box body, the water heating mechanism is arranged in the side wall of the box body, each chamber is provided with a humidifying disc, the middle part of each chamber is fixedly connected with one end of the supporting rod, the other end of the supporting rod is fixedly connected with the humidifying disc, the humidifying disc is a cube with an opening on one side, the humidity maintaining mechanism is arranged inside the box body, the CO2 generator is arranged inside the box body, one end of the first exhaust pipe is fixedly connected with the CO2 generator, one end of the first exhaust pipe, far away from the CO2 generator, is arranged at the upper end of the box body, the CO2 concentration maintaining mechanism, is economical and practical.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a constant-temperature and constant-humidity cell culture box.
Background
In the cell culture technology, the survival of cells needs to be simulated, and the temperature, the humidity and the CO2 concentration are required to be constant, so that the survival rate of the cells is ensured. In biological and medical research, cell culture chambers are common tools for in vitro cell culture, and are used for researching biological properties and molecular biological mechanisms of cells. In a living body, some cells have photosensitivity, that is, light with different wavelengths and different illumination intensities can induce and influence physiological or pathophysiological behaviors of the cells. Need mend in the culture process and change primary cell culture medium in the specified time, just so can accomplish cell culture, current mode is to take out the manual work and change primary cell culture medium with the household utensils that are equipped with the cell, and in the in-process that we need open the chamber door and get the inside household utensils of box, the inside temperature, humidity, CO2 concentration of box and the illumination condition all changed after opening the door, and then influenced the survival rate of cell.
Disclosure of Invention
The invention aims to provide a constant-temperature and constant-humidity cell culture box to solve the problems in the background technology.
In order to solve the technical problems, the invention provides the following technical scheme: a constant temperature and humidity cell culture box. The incubator comprises a incubator body, a chamber door, chambers, a water heating mechanism, a humidifying disc, a supporting rod, a humidity maintaining mechanism, a CO2 generator, a first exhaust pipe and a CO2 concentration maintaining mechanism, wherein one side of the incubator body is movably connected with the chamber door, three chambers with equal volume are arranged in the incubator body, the water heating mechanism is arranged in the side wall of the incubator body, each chamber is provided with the humidifying disc, the middle part of each chamber is fixedly connected with one end of the supporting rod, the other end of the supporting rod is fixedly connected with the humidifying disc, the humidifying disc is a cube with an open side, the humidity maintaining mechanism is arranged in the incubator body, the CO2 generator is arranged in the incubator body, one end of the first exhaust pipe is fixedly connected with the CO2 generator, one end of the first exhaust pipe, far away from the CO2 generator, is arranged at the upper end of the incubator body, the CO2 concentration maintaining mechanism, and the box door is coated with a shading material.
The inside cavity that is equipped with three volume and equals of box, can cultivate three kinds of cells in different periods simultaneously as required, the inside hot-water heating mechanism that is equipped with of box can keep warm three cavity simultaneously, the dish that humidifies is one side open-ended cube, be equipped with the pure water in the dish that humidifies, the evaporation through moisture reaches the purpose of keeping humidity in the box, CO2 generator is through an blast pipe to the internal CO2 of transmission box and keeps the indoor CO2 concentration, the chamber door is transparent material, scribble the illumination condition that light-shielding material reaches the box internal cell simultaneously on the chamber door. When the door needs to be opened to operate the inside of the box body, the humidity maintaining mechanism can automatically maintain the humidity inside the box body, and the CO2 concentration maintaining mechanism is used for maintaining the CO2 concentration inside the box body so as to ensure that cells inside the box body are not influenced.
Furthermore, the water heating mechanism comprises a power supply, a lead, a resistance wire, a first water pipe and a constant temperature mechanism, wherein the first water pipe is embedded into the inner wall of the box body, the resistance wire is arranged on the first water pipe, the two ends of the resistance wire are electrically connected with the power supply through the lead, and the constant temperature mechanism is arranged on the resistance wire.
The battery, resistance wire and wire form closed loop, and the resistance wire winding is on a water pipe, so flow through the winding have water in the water pipe of resistance wire can be heated, the water of heating can flow in a water pipe, and then hot water is with the inside heating of box. Inside heating the box through the temperature, unexpected circumstances such as outage appear in the incubator takes place, and the radiating rate of water is relatively slow, so cell survival rate in can the most probable protection incubator, when needs open the chamber door and operate, constant temperature mechanism can make the inside temperature of box rise, and then guarantees that the cultivation condition of cell does not receive the change.
Further, the constant temperature mechanism comprises a motor, a rotating shaft, a rotating mechanism, a connecting rod, an oil chamber, a spring, an oil pipe, an oil chamber, a connecting rod, a spring and a sliding rheostat, wherein the oil chamber is embedded in the inner wall of the box body, one end of the connecting rod is in sliding connection with the inside of the oil chamber inside the oil chamber, one end of the connecting rod in the oil chamber is fixedly connected with one end of the spring, the spring is far away from one end of the connecting rod and is fixedly connected with the bottom of the oil chamber, one end of the connecting rod is far away from one end of the oil chamber and is fixedly connected with the box door, one end of the oil pipe is communicated with the bottom of the oil chamber, the oil pipe is far away from one end of the oil chamber and is communicated with the oil chamber, the connecting rod in the oil chamber is arranged inside, and is in sliding contact with the inner wall of the oil chamber inside the oil chamber, no. two springs of one end fixed connection of connecting rod in No. two oil rooms, No. two grease chamber bottoms of spring other end fixed connection No. two, No. two grease chamber one end fixed connection slide rheostat are kept away from to No. two connecting rods, slide rheostat establishes ties in resistance wire place circuit, a pivot of motor output shaft fixed connection, motor one end is kept away from in a pivot and is passed a water pipe lateral wall, a pivot is at the inside fixed connection rotary mechanism of a water pipe.
When the box door is opened, the first connecting rod slides in the first oil chamber under the driving of the box door, so that hydraulic oil in the second oil chamber enters the first oil chamber through the first oil pipe, the second connecting rod moves towards the second oil chamber, one end of the second connecting rod is fixedly connected with the sliding rheostat, and the resistance of a circuit connected with the sliding rheostat is reduced, so that the current in the resistance wire is increased, the power of the resistance wire is increased, the temperature of water in the first water pipe is increased, meanwhile, the motor drives the first rotating shaft to rotate, the first rotating shaft drives the rotating mechanism to rotate in the first water pipe, water in the first water pipe is driven by the rotating mechanism to rapidly flow, and further heated water is rapidly flowed to each place of the first water pipe, so that the temperature in the box body is increased, namely, when the box door is opened, the living temperature of cells in the incubator is not changed, and the survival conditions of the cells, the survival rate is improved.
Further, rotary mechanism includes No. three grease chambers, No. two oil pipes, goes up the rotor plate, lower rotor plate and No. three springs, No. three grease chambers are the ring form, No. three grease chamber fixed connection pivot, be equipped with No. two oil pipe intercommunications between No. three grease chambers and No. two grease chambers, No. three last fixed connection lower rotor plate of grease chamber, it is equipped with the recess to keep away from No. three grease chamber one end down on the rotor plate, go up rotor plate one end in the recess and lower rotor plate inner wall sliding contact, go up rotor plate one end fixed connection No. three springs of recess, rotor plate under No. three spring other end fixed connection.
After the chamber door is opened, No. two springs are compressed in No. two grease chambers, under the effect of No. two connecting rods, hydraulic oil passes through No. two oil pipes and gets into No. three grease chambers, lower rotor plate and No. three grease chambers intercommunication, and then hydraulic oil gets into rotor plate extrusion upper rotor plate down, so upper rotor plate receives thrust and removes to the direction of keeping away from No. three grease chambers, and then rotary mechanism's blade area increases, area of contact with water in a water pipe increases, so rotary mechanism can drive more rivers and flow under the drive of motor, the water of heating is the increase of flow rate in a water pipe, so the hydroenergy of heating is faster flow everywhere to a water pipe, so when the chamber door is opened, the cell temperature survival condition inside the incubator does not receive the change, make the cell survival condition stable, improve the survival rate.
Further, humidity holding mechanism includes the water tank, a slider, No. four springs, No. three oil pipe and No. two water pipes, the water tank is fixed on the box inner wall, the inside slider that is equipped with of water tank, No. four springs of slider one end fixed connection, No. four spring other end fixed connection water tank inner wall upper ends, No. three oil pipe of water tank upper end fixed connection, No. three oil pipe keep away from the grease chamber of water tank one end fixed connection, No. two water pipes of water tank lower extreme fixed connection, No. two water pipes keep away from water tank one end fixed connection humidification dish side.
When the box door is opened, the amount of hydraulic oil in the third oil chamber is increased, so that a part of hydraulic oil enters the upper end of the water tank through the third oil pipe, the first sliding block is extruded in the water tank, the first sliding block moves downwards to enable water in the water tank to enter the humidifying disc through the second water pipe, the water content in the humidifying disc is increased, the upper surface area of the humidifying disc is unchanged due to the fact that the humidifying disc is a cube with one open side, the thickness of the water in the humidifying disc is increased, light outside the box door enters the culture dish through the humidifying disc after the box door is opened, the height of the water in the humidifying disc is increased at the moment, the refraction amount of the light is changed, the light entering the culture dish is effectively shielded, the height and the area of the humidifying disc can be controlled to keep the illumination condition of cells unchanged all the time, the growth environment of the cells cannot be changed due to the opening of the box door, the survival condition of the cells is stable, and the survival rate, and when the water in the humidifying disc increases, the increased water can drive the water in the humidifying disc to fluctuate, so that the contact area with air is increased, and the humidity in the box body is increased to a certain degree.
Furthermore, CO2 concentration holding mechanism includes No. two blast pipes and valve mechanism, No. two blast pipes one end fixed connection CO2 generators, No. two blast pipes keep away from CO2 generator one end and are located the humidification dish top, be equipped with valve mechanism on No. two blast pipes.
The air displacement of the second exhaust pipe is controlled by the valve mechanism, so that when the box door is opened, the opening size of the valve mechanism is automatically controlled, the inner diameter of the second exhaust pipe can be increased by the valve mechanism, the discharge amount of CO2 is increased, the concentration of CO2 in the environment where the cells are located is kept consistent, the survival conditions of the cells are stable, and the survival rate of the cells is improved. Because the end of the second exhaust pipe, which is far away from the CO2 generator, is positioned above the humidifying disc, when the valve mechanism increases the emission of CO2, the air flow rate above the humidifying disc is increased, the evaporation speed of water in the humidifying disc is increased, and the humidity in the chamber is increased.
Further, the valve mechanism comprises a valve body, a fourth oil chamber, a fourth oil pipe, a third connecting rod, a rack, a gear, a valve rod, a limiting rod, a first sleeve and a baffle plate, wherein the side face of the fourth oil chamber is fixedly connected with the outer wall of the valve body, one end of the third connecting rod is in sliding contact with the inside of the fourth oil chamber in the fourth oil chamber, one end of the third connecting rod, which is far away from the fourth oil chamber, is fixedly connected with the rack, the upper end of the valve body is provided with a first hole, the valve rod extends into the valve body from the first hole, one end of the valve rod in the valve body is provided with a thread, the first sleeve is provided with a groove, an internal thread is arranged in the groove, one end of the valve rod, which is inside the valve body, extends into the groove to be in threaded connection with the first sleeve, the bottom of the first sleeve is provided with the baffle plate, the side, no. four oil pipe one end fixed connection grease chamber No. four, No. four oil pipe keeps away from No. four grease chamber one end fixed connection grease chamber No. one.
When the box door is opened, the volume of the hydraulic oil on one side in the first oil chamber is reduced, and the volume of the hydraulic oil on the other side is increased, so that the hydraulic oil far away from the first oil pipe in the first oil chamber enters the fourth oil chamber from the fourth oil pipe, the volume of the hydraulic oil in the fourth oil chamber is increased, the third connecting rod is pushed to slide in the fourth oil chamber, the third connecting rod pushes the rack to move, the rack is meshed with the gear, the gear rotates, the gear drives the valve rod to rotate, one end of the valve rod far away from the gear is fixedly connected with the first sleeve in a threaded manner, the first sleeve moves towards the gear, the baffle is driven to approach towards the gear, at the moment, CO2 in the second exhaust pipe is discharged into the chamber from a gap between the valve body and the baffle, when the opening angle of the box door is larger, the gap between the baffle and the valve body is larger, and, the concentration of CO2 in the chamber is not changed along with the opening of the box door, the stability of the growth environment of the cells is ensured, and the survival rate of the cells is improved.
Compared with the prior art, the invention has the following beneficial effects: the constant temperature inside the box body is ensured by arranging the water heating mechanism in the side wall of the box body, and meanwhile, the constant temperature mechanism is arranged in the water heating mechanism, so that when the door of the box body is opened, the temperature inside the box body is not influenced; the humidifying disc is arranged in the box body to ensure constant humidity in the box body, the humidity maintaining mechanism is arranged on one side of the humidifying disc, so that the constant humidity in the box body can be ensured when the box door is opened, the illumination environment of cells in the box body is changed while the humidity is changed, and the cells are prevented from being damaged by natural light after the box door is opened; a CO2 generator is arranged in the box body, which can meet the supply of CO2 for the survival of cells in the box body, a CO2 concentration keeping mechanism is arranged above the humidifying plate to keep the concentration of CO2 in the box body unchanged, and the CO2 concentration keeping mechanism plays a role in promoting the humidity in the box body while working. The invention is scientific and reasonable, simple in structure, economical and practical.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a perspective view of the overall mechanism of the present invention;
FIG. 2 is a schematic front view of the case of the present invention;
FIG. 3 is a structural diagram of the position of the first oil chamber in the box body;
FIG. 4 is a schematic view of the water heating mechanism of the present invention;
FIG. 5 is a schematic view of the humidity maintaining mechanism of the present invention;
FIG. 6 is a schematic view of a rotary mechanism of the present invention;
FIG. 7 is a schematic view of the valve mechanism of the present invention;
in the figure: 1-a box body; 2-a box door; 3-a chamber; 4-a water heating mechanism; 41-power supply; 42-a wire; 43-resistance wire; 44-a first water pipe; 451-electric motor; 452-a rotating shaft; 453-a rotation mechanism; 454-link No. one; 455-oil chamber one; 456-spring number one; 457-oil pipe number one; 458-second oil chamber; 459-link No. two; 460-spring two; 461-sliding rheostat; 462-oil chamber III; 463-oil line II; 464-upper rotating sheet; 465-lower rotary piece; 466-three spring; 5-a humidifying disc; 6-supporting rods; 7-a humidity maintaining mechanism; 71-a water tank; 72-first slide block; 73-four spring; 74-third tubing; 75-second water pipe; an 8-CO2 generator; 9-exhaust pipe I; 101-exhaust pipe II; 102-a valve mechanism; 103-a valve body; number 104-four oil chamber; 105-four tubing; 106-connecting rod III; 107-rack; 108-gear; 109-a valve stem; 110-a limiting rod; sleeve No. 111-one; 112-baffle.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 7, the present invention provides a technical solution: a constant temperature and humidity cell culture box. The incubator comprises an incubator body 1, an incubator door 2, chambers 3, a water heating mechanism 4, a humidifying disc 5, a support rod 6, a humidity maintaining mechanism 7, a CO2 generator 8, a first exhaust pipe 9 and a CO2 concentration maintaining mechanism, wherein one side of the incubator body 1 is movably connected with the incubator door 2, three chambers 3 with equal volume are arranged inside the incubator body 1, the water heating mechanism 4 is arranged in the side wall of the incubator body 1, each chamber 3 is provided with a humidifying disc 5, the middle part of each chamber 3 is fixedly connected with one end of the support rod 6, the other end of the support rod 6 is fixedly connected with the humidifying disc 5, the humidifying disc 5 is a cube with an opening on one side, the humidity maintaining mechanism 7 is arranged inside the incubator body 1, the CO2 generator 8 is arranged inside the incubator body 1, one end of the first exhaust pipe 9 is fixedly connected with the CO2 generator 8, one end of the first exhaust pipe 9, which is far away from the CO 539, the cabinet body 1 is internally provided with a CO2 concentration maintaining mechanism, the cabinet door 2 is made of transparent materials, and the cabinet door 2 is coated with shading materials. The inside cavity 3 that is equipped with three volume and equals of box 1, can cultivate three kinds of cells simultaneously as required, 1 inside hot-water heating mechanism 4 that is equipped with of box can keep warm three cavity 3 simultaneously, humidification dish 5 is one side open-ended cube, be equipped with the pure water in the humidification dish 5, reach the purpose of keeping humidity in the box 1 through the evaporation of moisture, CO2 generator 8 keeps the CO2 concentration in the cavity 3 through an exhaust pipe 9 to the box 1 internal emission CO2, chamber door 2 is transparent material, scribble the light-shielding material on chamber door 2 simultaneously and reach the illumination condition of cell in the box 1. When the door 2 needs to be opened to operate the inside of the cabinet 1, the humidity maintaining mechanism 7 can automatically maintain the humidity inside the cabinet 1, and the CO2 concentration maintaining mechanism is used for maintaining the CO2 concentration inside the cabinet 1 to ensure that the cells inside the cabinet are not affected.
The water heating mechanism 4 comprises a power supply 41, a lead 42, a resistance wire 43, a first water pipe 44 and a constant temperature mechanism, wherein the first water pipe 44 is embedded into the inner wall of the box body 1, the resistance wire 43 is arranged on the first water pipe 44, two ends of the resistance wire 43 are electrically connected with the power supply 41 through the lead 42, and the constant temperature mechanism is arranged on the resistance wire 43. The battery, the resistance wire 43 and the lead 42 form a closed loop, the resistance wire 43 is wound on the first water pipe 44, so that water flowing through the first water pipe 44 wound with the resistance wire 43 can be heated, the heated water can flow in the first water pipe 44, and then the interior of the box body 1 is heated by the hot water. Through temperature heating box 1 inside, unexpected circumstances such as outage appear in the incubator takes place, and the radiating rate of water is slower, so cell survival rate in can the most probable protection incubator, when needs open chamber door 2 and operate, constant temperature mechanism can make 1 inside temperature of box rise, and then guarantees that the cultivation condition of cell does not receive the change.
The constant temperature mechanism comprises a motor 451, a first rotating shaft 452, a rotating mechanism 453, a first connecting rod 454, a first oil chamber 455, a first spring 456, a first oil pipe 457, a second oil chamber 458, a second connecting rod 459, a second spring 460 and a sliding rheostat 461, wherein the first oil chamber 455 is embedded in the inner wall of the box body 1, one end of the first connecting rod 454 is connected with the inside of the first oil chamber 455 in a sliding manner inside the first oil chamber 455, one end of the first connecting rod 454 in the first oil chamber 455 is fixedly connected with one end of the first spring 456, which is far away from the first connecting rod 454, is fixedly connected with the bottom of the first oil chamber 455, one end of the first oil pipe 457, which is far away from the first oil chamber 455, is connected with the second oil chamber 458, and the second connecting rod 459 is arranged inside the second oil chamber 458, the second connecting rod 459 is in sliding contact with the inner wall of the second oil chamber 458 inside the second oil chamber 458, one end of the second connecting rod 459 inside the second oil chamber 458 is fixedly connected with a second spring 460, the other end of the second spring 460 is fixedly connected with the bottom of the second oil chamber 458, one end, far away from the second oil chamber 458, of the second connecting rod 459 is fixedly connected with a sliding rheostat 461, the sliding rheostat 461 is connected in series in a circuit where the resistance wire 43 is located, an output shaft of the motor 451 is fixedly connected with a first rotating shaft 452, one end, far away from the motor 451, of the first rotating shaft 452 penetrates through the side wall of the first water pipe 44, and the first rotating shaft 452 is fixedly connected with a rotating mechanism 453 inside the. When the box door 2 is opened, the first connecting rod 454 slides in the first oil chamber 455 under the driving of the box door 2, so that the hydraulic oil in the second oil chamber 458 enters the first oil chamber 455 through the first oil pipe 457, the second connecting rod 459 moves towards the second oil chamber 458, one end of the second connecting rod 459 is fixedly connected with the sliding rheostat 461, and the resistance of the sliding rheostat 461 connected to the circuit is reduced, so that the current in the resistance wire 43 is increased, the power of the resistance wire 43 is increased, the temperature of the water in the first water pipe 44 is increased, meanwhile, the motor 451 drives the first rotating shaft 452 to rotate, the first rotating shaft 452 drives the rotating mechanism 453 to rotate in the first water pipe 44, the water in the first water pipe 44 is driven by the rotating mechanism 453 to rapidly flow, so that the heated water rapidly flows to all places of the first water pipe 44, and the temperature inside the box body 1 can be increased, namely, when the box door 2 is opened, the survival temperature of the cells in the incubator is not changed, so that the survival conditions of the cells are stable, and the survival rate is improved.
The rotating mechanism 453 comprises a third oil chamber 462, a second oil pipe 463, an upper rotating sheet 464, a lower rotating sheet 465 and a third spring 466, the third oil chamber 462 is annular, the third oil chamber 462 is fixedly connected with a first rotating shaft 452, the second oil pipe 463 is arranged between the third oil chamber 462 and the second oil chamber 458 and communicated with each other, the third oil chamber 462 is fixedly connected with the lower rotating sheet 465, a groove is formed in one end, far away from the third oil chamber 462, of the lower rotating sheet 465, one end of the upper rotating sheet 464 is in sliding contact with the inner wall of the lower rotating sheet 465 in the groove, one end of the upper rotating sheet 464 is fixedly connected with the third spring 466 in the groove, and the other end of the third spring 466 is fixedly connected with the lower rotating sheet 465. After the chamber door 2 is opened, the second spring 460 in the second oil chamber 458 is compressed, under the action of the second connecting rod 459, hydraulic oil enters the third oil chamber 462 through the second oil pipe 463, the lower rotating sheet 465 is communicated with the third oil chamber 462, and then the hydraulic oil enters the lower rotating sheet 465 to extrude the upper rotating sheet 464, so that the upper rotating sheet 464 moves in the direction away from the third oil chamber 462 under the thrust, the blade area of the rotating mechanism 453 is increased, the contact area with water in the first water pipe 44 is increased, so that the rotating mechanism 453 drives more water to flow under the driving of the motor 451, the flow speed of the heated water in the first water pipe 44 is increased, the heated water can flow to all parts of the first water pipe 44 more quickly, when the chamber door 2 is opened, the survival temperature of cells in the incubator is not changed, the survival condition of the cells is stable, and the survival rate of the cells is improved.
The humidity maintaining mechanism 7 comprises a water tank 71, a slider 72, a spring 73, a third oil pipe 74 and a second water pipe 75, wherein the water tank 71 is fixed on the inner wall of the box body 1, the slider 72 is arranged in the water tank 71, one end of the slider 72 is fixedly connected with the spring 73, the other end of the spring 73 is fixedly connected with the upper end of the inner wall of the water tank 71, the upper end of the water tank 71 is fixedly connected with the third oil pipe 74, the third oil pipe 74 is far away from one end of the water tank 71 and is fixedly connected with an oil chamber 455, the lower end of the water tank 71 is fixedly connected with the second water pipe 75, and the second water pipe 75 is far away from one end of the water tank 71 and is fixedly connected. When the chamber door 2 is opened, the amount of hydraulic oil in the third oil chamber 462 is increased, so that a part of hydraulic oil enters the upper end of the water tank 71 through the third oil pipe 74, the first slide block 72 is extruded in the water tank 71, the first slide block 72 moves downwards to enable water in the water tank 71 to enter the humidifying disc 5 through the second water pipe 75, the water content in the humidifying disc 5 is increased, the humidifying disc 5 is a cube with one open side, the upper surface area of the humidifying disc 5 is unchanged, the thickness of the water in the humidifying disc 5 is increased, therefore, after the chamber door is opened, light outside the chamber door enters a culture dish through the humidifying disc 5, the height of the water in the humidifying disc 5 is increased, the refraction amount of the light is changed, the light entering the culture dish is effectively shielded, the height and the area of the humidifying disc 5 are controlled to keep the illumination condition of cells constantly unchanged, and the growth environment of the cells cannot be changed due to the opening of the chamber door 2, and then make the survival condition of cell stable, improved the survival rate of cell, and when the water in humidification dish 5 increased, the water that increases can drive the water in humidification dish 5 and produce undulant, increased the area of contact with the air, so increased the inside humidity of box 1 on a certain stratification degree.
The CO2 concentration maintaining mechanism comprises a second exhaust pipe 101 and a valve mechanism 102, one end of the second exhaust pipe 101 is fixedly connected with the CO2 generator 8, one end, far away from the CO2 generator 8, of the second exhaust pipe 101 is located above the humidifying disc 5, and the valve mechanism 102 is arranged on the second exhaust pipe 101. The valve mechanism 102 controls the exhaust volume of the second exhaust pipe 101, so when the box door 2 is opened, the valve mechanism 102 automatically controls the opening size, and the valve mechanism 102 increases the inner diameter of the second exhaust pipe 101, so that the discharge volume of CO2 is increased, the concentration of CO2 in the environment where the cells are located is kept consistent, the survival conditions of the cells are stable, and the survival rate of the cells is improved. Since the end of the second exhaust pipe 101 away from the CO2 generator 8 is located above the humidifying disc 5, when the valve mechanism 102 increases the emission of CO2, the air flow rate above the humidifying disc 5 is increased, the evaporation rate of moisture in the humidifying disc 5 is increased, and the humidity in the chamber 3 is increased.
The valve mechanism 102 comprises a valve body 103, a fourth oil chamber 104, a fourth oil pipe 105, a third connecting rod 106, a rack 107, a gear 108, a valve rod 109, a limiting rod 110, a first sleeve 111 and a baffle 112, wherein the side surface of the fourth oil chamber 104 is fixedly connected with the outer wall of the valve body 103, one end of the third connecting rod 106 is in sliding contact with the inside of the fourth oil chamber 104 in the fourth oil chamber 104, the third connecting rod 106 is far away from one end of the fourth oil chamber 104 and is fixedly connected with the rack 107, the upper end of the valve body 103 is provided with a first hole, the valve rod 109 extends into the valve body 103 from the first hole, one end of the valve rod 109 in the valve body 103 is provided with a thread, the first sleeve 111 is provided with a groove, an internal thread is arranged in the groove, one end of the valve rod 109 in the valve body 103 extends into the groove to be in threaded connection with the first sleeve 111, the bottom of the first sleeve, the valve rod 109 is kept away from one end of the valve body 103 and is sleeved with a gear 108, the rack 107 is meshed with the gear 108, the fourth oil pipe 105 one end is fixedly connected with the fourth oil chamber 104, and the fourth oil pipe 105 is kept away from the first oil chamber 455 of the fourth oil chamber 104 one end fixed connection. When the box door 2 is opened, the volume of the hydraulic oil on one side in the first oil chamber 455 is reduced, and the volume of the hydraulic oil on the other side is increased, so that the hydraulic oil in the first oil chamber 455, which is far away from the first oil pipe 457, enters the fourth oil chamber 104 from the fourth oil pipe 105, the volume of the hydraulic oil in the fourth oil chamber 104 is increased, the third connecting rod 106 is pushed to slide in the fourth oil chamber 104, the third connecting rod 106 pushes the rack 107 to move, the rack 107 is meshed with the gear 108, the gear 108 rotates, the gear 108 drives the valve rod 109 to rotate, one end of the valve rod 109, which is far away from the gear 108, is fixedly connected with the first sleeve 111 in a threaded manner, the first sleeve 111 moves in a direction close to the gear 108, the baffle 112 is driven to approach the gear 108, at this time, the CO2 in the second exhaust pipe 101 is discharged into the chamber 3 from a gap between the valve body, the larger the gap between the baffle 112 and the valve body 103 is, the concentration of CO2 in the chamber 3 can be supplemented, so that the concentration of CO2 in the chamber 3 is not changed along with the opening of the box door 2, the stability of the growth environment of cells is ensured, and the survival rate of the cells is improved.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. The utility model provides a constant temperature and humidity cell culture case which characterized in that: the incubator comprises a box body (1), a box door (2), chambers (3), a water heating mechanism (4), a humidifying disc (5), a support rod (6), a humidity maintaining mechanism (7), a CO2 generator (8), a first exhaust pipe (9) and a CO2 concentration maintaining mechanism, wherein the box door (2) is movably connected to one side of the box body (1), three chambers (3) with equal volume are arranged inside the box body (1), the water heating mechanism (4) is arranged in the side wall of the box body (1), each chamber (3) is provided with the humidifying disc (5), the middle of each chamber (3) is fixedly connected with one end of the support rod (6), the other end of the support rod (6) is fixedly connected with the humidifying disc (5), the humidifying disc (5) is a cube with an opening on one side, the humidity maintaining mechanism (7) is arranged inside the box body (1), and the CO2 generator (8) is arranged inside the box body (1), one end of the first exhaust pipe (9) is fixedly connected with a CO2 generator (8), one end, far away from the CO2 generator (8), of the first exhaust pipe (9) is arranged at the upper end of the box body (1), a CO2 concentration maintaining mechanism is arranged inside the box body (1), the box door (2) is made of transparent materials, and light shielding materials are coated on the box door (2);
the water heating mechanism (4) comprises a power supply (41), a lead (42), a resistance wire (43), a first water pipe (44) and a constant temperature mechanism, wherein the first water pipe (44) is embedded into the inner wall of the box body (1), the resistance wire (43) is arranged on the first water pipe (44), two ends of the resistance wire (43) are electrically connected with the power supply (41) through the lead (42), and the constant temperature mechanism is arranged on the resistance wire (43);
the constant temperature mechanism comprises a motor (451), a first rotating shaft (452), a rotating mechanism (453), a first connecting rod (454), a first oil chamber (455), a first spring (456), a first oil pipe (457), a second oil chamber (458), a second connecting rod (459), a second spring (460) and a sliding rheostat (461), wherein the first oil chamber (455) is embedded in the inner wall of the box body (1), one end of the first connecting rod (454) is in sliding connection with the inside of the first oil chamber (455) inside the first oil chamber (455), one end of the first connecting rod (454) in the first oil chamber (455) is fixedly connected with one end of the first spring (456), one end of the first spring (456) far away from one end of the first connecting rod (454) is fixedly connected with the bottom of the first oil chamber (455), one end of the first connecting rod (454) far away from one end of the first oil chamber (455) is fixedly connected with the box door (2), one end of the first oil pipe (457) is communicated with the bottom of the first, one end of the first oil pipe (457) far away from the first oil chamber (455) is communicated with a second oil chamber (458), a second connecting rod (459) is arranged in the second oil chamber (458), the second connecting rod (459) is in sliding contact with the inner wall of the second oil chamber (458) in the second oil chamber (458), one end of the second connecting rod (459) in the second oil chamber (458) is fixedly connected with a second spring (460), the other end of the second spring (460) is fixedly connected with the bottom of the second oil chamber (458), one end of the second connecting rod (459) far away from the second oil chamber (458) is fixedly connected with a sliding rheostat (461), the slide rheostat (461) is connected in series in a circuit where the resistance wire (43) is positioned, an output shaft of the motor (451) is fixedly connected with a first rotating shaft (452), one end of the first rotating shaft (452) far away from the motor (451) penetrates through the side wall of the first water pipe (44), the first rotating shaft (452) is fixedly connected with the rotating mechanism (453) in the first water pipe (44).
2. The constant temperature and humidity cell culture box according to claim 1, wherein: the rotating mechanism (453) comprises a third oil chamber (462), a second oil pipe (463), an upper rotating sheet (464), a lower rotating sheet (465) and a third spring (466), the third oil chamber (462) is annular, the third oil chamber (462) is fixedly connected with a first rotating shaft (452), a second oil pipe (463) is arranged between the third oil chamber (462) and the second oil chamber (458) to be communicated, the third oil chamber (462) is fixedly connected with the lower rotating sheet (465), one end, far away from the third oil chamber (462), of the lower rotating sheet (465) is provided with a groove, one end of the upper rotating sheet (464) is in sliding contact with the inner wall of the lower rotating sheet (465) in the groove, one end of the upper rotating sheet (464) in the groove is fixedly connected with the third spring (466), and the other end of the third spring (466) is fixedly connected with the lower rotating sheet (465).
3. The constant temperature and humidity cell culture box according to claim 1, wherein: humidity hold mechanism (7) are including water tank (71), slider (72), No. four spring (73), No. three oil pipe (74) and No. two water pipe (75), water tank (71) are fixed on box (1) inner wall, inside slider (72) that is equipped with of water tank (71), No. four spring (73) of slider (72) one end fixed connection, No. four spring (73) other end fixed connection water tank (71) inner wall upper end, No. three oil pipe (74) of water tank (71) upper end fixed connection, No. three oil pipe (74) keep away from water tank (71) one end fixed connection grease chamber (455), No. two water pipe (75) of water tank (71) lower extreme fixed connection, No. two water pipe (75) keep away from water tank (71) one end fixed connection humidification dish (5) side.
4. The constant temperature and humidity cell culture box according to claim 1, wherein: the CO2 concentration keeping mechanism comprises a second exhaust pipe (101) and a valve mechanism (102), one end of the second exhaust pipe (101) is fixedly connected with a CO2 generator (8), one end, far away from the CO2 generator (8), of the second exhaust pipe (101) is located above the humidifying disc (5), and the valve mechanism (102) is arranged on the second exhaust pipe (101).
5. The constant temperature and humidity cell culture box according to claim 4, wherein: valve mechanism (102) includes valve body (103), No. four grease chambers (104), No. four oil pipe (105), No. three connecting rod (106), rack (107), gear (108), valve rod (109), gag lever post (110), sleeve (111) and baffle (112), No. four grease chambers (104) side fixed connection valve body (103) outer wall, No. three connecting rod (106) one end in No. four grease chambers (104) and the inside sliding contact of No. four grease chambers (104), No. three connecting rod (106) are kept away from No. four grease chambers (104) one end fixed connection rack (107), valve body (103) upper end is equipped with a hole, valve rod (109) are inside from a downthehole stretching into valve body (103), valve rod (109) are equipped with the screw thread in valve body (103), be equipped with the recess on sleeve (111), the inside internal thread that is equipped with of recess, valve rod (109) stretch into recess and sleeve (111) threaded connection in No. one end of valve body (103) inside The utility model discloses a sleeve (111) bottom is equipped with baffle (112), be equipped with gag lever post (110) on sleeve (111) lateral wall, valve body (103) one end is kept away from in valve rod (109) and one is served the cover and is equipped with gear (108), rack (107) and gear (108) meshing, No. four oil pipe (105) one end fixed connection No. four grease chambers (104), No. four oil pipe (105) keep away from No. four grease chambers (104) one end fixed connection grease chamber (455).
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| CN202110543812.0A CN112980687B (en) | 2021-05-19 | 2021-05-19 | Constant temperature and humidity cell culture box |
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Cited By (1)
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| CN113999813A (en) * | 2021-10-12 | 2022-02-01 | 云南和泽西南生物科技有限公司 | Primary culture method of umbilical cord mesenchymal stem cells |
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