CN2293046Y - One-shot temp compensation biosensor - Google Patents

Abstract

The utility model relates to a one-shot temp compensation biosensor, which is characterized in that the utility model can avoid the influence of environmental temperature change to the measuring accuracy of a bioprobe. Each batch of bioprobe is matched with a normal probe which is composed of a thermal resistor, an ordinary resistor, etc., after connected with the biosensor, the response output under a certain temperature is corresponding to the response value of the batch of bioprobe to a measured object with standard concentration under the same temperature condition, before a sample is measured by the bioprobe, the normal probe is used for scaling the biosensor, and measurement error induced by the difference between test environment temperature and bioprobe calibrating temperature before leaving a factory can be avoided.

Description

Disposable temperature compensating type biology sensor

The utility model is disposable temperature compensating type biology sensor, belongs to biological technical field.

Said disposable biological sensor is meant can not or not need reusable biology sensor, as print enzyme electrode, system adopts screen printing technique that enzyme, amboceptor and base electrode are printed on the plastic substrate and forms, energy homogeneous, batch process at low cost, electrode was used promptly and was thrown away, and used very convenient.Britain company patents at first, the fast measuring that can be used for biochemical parameters such as body fluid sugar, fermentable carbohydrate, alcohols, amino acids, do not reuse and mean and can not be on same bioprobe have not only demarcated but also measured the known two kinds of demarcation modes that have: a kind of is the stability that improves production technology as far as possible, make different series-produced bioprobes have good interchangeability, bioprobe was demarcated through sampling before dispatching from the factory, it is qualified that the conformance with standard response is considered as, and this mode be cannot say for sure to demonstrate,prove technically; Another kind is that sampling is demarcated in series-produced bioprobe, select the resistance of a fixed resistance value for use according to calibrating parameters, be packaged into the standard probe and deliver to the user in company with this batch bioprobe, when the user inserts biology sensor instrument socket with this standard probe, instrument is just imported the calibrating parameters of this batch bioprobe, each series-produced bioprobe all is furnished with the standard probe of respective resistance values, can avoid the bioprobe differences between batches thus and the error at measurment that causes.But, because enzyme reaction temperature influence always, the error at measurment that environment temperature causes with the different of producer timing signal temperature when above-mentioned dual mode all can not avoid the user to measure, its temperature difference is big more, and error is big more.

Task of the present invention provides a kind of disposable temperature compensating type biology sensor, makes the disposable biological probe under Various Seasonal, region and variation of ambient temperature round the clock, is not acted upon by temperature changes, and guarantees to measure accuracy.

The scheme of its solution is: bioprobe has certain temperature coefficient, therefore its calibration value (refers under certain specified requirements, bioprobe is to the response of normal concentration measured object) different under different temperatures, after having determined temperature funtion, selected temperature funtion with bioprobe has the thermal sensing element (as thermistor) of corresponding temperature funtion, the conventional, electric-resistance that the resistance of connecting again is fixing, constitute a standard probe, purpose is to make this standard probe after inserting the biology sensor instrument under the different temperature, its output corresponding to bioprobe under the same conditions to the calibration value of normal concentration measured object.Every batch of bioprobe all is furnished with this standard probe, during use, demarcate the biology sensor instrument with this standard probe earlier, make instrument read in the calibration value of bioprobe under the detected temperatures, again with bioprobe to sample determination, just can solve because the error at measurment that the variation of environment temperature causes.

As previously mentioned, existing disposable biological sensor is measured and is acted upon by temperature changes easily.By comparison, the utility model can overcome this influence, guarantees the accuracy of mensuration, thereby has tangible advantage.

Embodiment of the present utility model is described below:

Accompanying drawing 1 is a kind of disposable biological sensor of the present invention (bioprobe is an enzyme electrode), main frame 1 (long 12cm, wide 9cm, high 1cm), and by display screen 2, keyswitch 3 and enzyme electrode 4 (long 3.8cm, wide 0.7cm, thick 0.2cm) are formed.Normal electrode 5 is by fixed resistance 6, thermistor 7, and joint 9 and bus 8 are formed, and will be connected with main frame 1 by joint 9.Enzyme electrode 4 is by electrode 10, and joint 11 and bus 12 are formed, and will be connected with main frame 1 by joint 11.

Accompanying drawing 2 is testing circuit schematic diagrams of a kind of disposable biological sensor instrument of the present invention, by complete machine power supply 1, penetrates grade follower 2, differential amplifier 3, power amplifier 4, V/F converter 5, MCS51 single-chip microcomputer 6, compositions such as LCD 7 and electrode interface 8.

Enzyme electrode is made: be printed on plastic basis material (as PVC) surface with commercially available silver-colored electrically conductive ink with the method for serigraphy and form one deck conduction silver bar, print the commercially available conductive carbon paste of one deck after the drying thereon, the formation basic electrode; Dry back is with containing a certain proportion of glucose oxidase and amboceptor (as ferrocene and derivant thereof, benzoquinones, the potassium ferricyanide, TTF, TCNQ etc., ratio is 5%-10%) suspension, be fixed in the basic electrode surface with instillation or method of printing, can form the organized enzyme electrode.

The temperature compensation mode of enzyme electrode: the temperature funtion of the enzyme electrode of making of said method is: lgI=a/T+b (I: be the response output current value (uA) of enzyme electrode; T: be thermodynamic temperature (K); A, b is respectively constant).With the 5mmol/L glucose solution is specimen, and in the time of 15 degrees centigrade, the calibration value of certain batch enzyme electrode is a 10uA/5mM glucose.Select for use the resistance RI of fixed resistance A to be: 19.5 kilo-ohms, select thermistor B for use, the resistance R2 in the time of its 15 degrees centigrade is: 5.6 kilo-ohms, temperature funtion is: lgR=a ₂/ T+b ₂, A and B are composed in series normal electrode C.The resistance R3 of C in the time of 15 degrees centigrade is: 25.1 kilo-ohms, and output current I '=U/R3=9.96uA (U is the constant voltage of 0.25V).The time being calculated as of glucose solution measured value: measured value=enzyme electrode under certain temperature to response * 5/ calibration value of glucose solution.Compensate as calibration value in the output under the different temperatures with normal electrode, compensation result sees attached list 1, and uncompensated result is to be that calibration value calculates with enzyme electrode response to the 5mmol/L glucose solution 15 degrees centigrade the time in the table.The result shows that this instrument can significantly reduce the error that causes because of temperature effect.

------instrument reads in calibration value automatically, and---the insertion enzyme electrode---drips sample---, and instrument is measured display result to enzyme electrode instrument trace routine: start automatically to insert normal electrode.

Claims (1)

1. A kind of disposable temperature compensating type biology sensor, it is characterized in that: every series-produced bioprobe is furnished with a standard probe, this standard is popped one's head in by thermal sensing element (as thermistor, fixed resistance and bus and joint are formed, one end of fixed resistance is connected with a utmost point of joint by the lead bar, the other end is connected with an end of thermistor by the lead bar, the other end of thermistor is connected with another utmost point of joint by the lead bar, after the start, the standard probe is connected with the socket of biology sensor instrument main frame by its joint, thereby subsequent measurements is demarcated and temperature compensation.

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