CN2250810Y - Cell crusher - Google Patents

Abstract

The utility model relates to a high-performance cell crusher, which improves the sealing structure. An irrigated and cooling distributor ring is arranged in the stuffing, and a pressure conduit which is communicated with the middle for water injection is arranged on the distributor ring. Thereby, glass chips with high speed can be prevented from entering the sealing position of a main shaft. The abrasion of the main shaft and shaft seal materials can be avoided, and the service life of the cell crusher is prolonged. The utility model has the advantages of simple structure, easy manufacture, long service life, and low cost. Milling micro powder is prevented from entering a rotary seal component by pressure, and thereby, the reliability of sealing is achieved. A novel high-performance cell crusher is ensured to be continuously and smoothly operated.

Description

Biomixer

The utility model relates to a kind of biomixer, is applicable to bioengineering, engineered breaking-wall cell, in order to the separation and Extraction intracellular organic matter.

In prior art, there are high-pressure homogenization method and pearl to grind method with Mechanical Method is cytoclastic.The high pressure homogenizer that the used equipment of high-pressure homogenization method is made up of high-pressure pump and homogenate valve, need broken cell suspending liquid under tens MPa high pressure, from the annular space of the spool of homogenate valve and valve seat,, shear and cell membrane and film are torn in the collision effect by strong fluid power with the high speed ejection of per second hundreds of rice.The industrial production capacity of this method has reached 9000l/h, but generally need be multistage with this method smudge cells, and each circulation is gone to toward carrying out the inter-stage cooling, and the temperature of reduction slurries is to keep cell activity.But bulk or filamentous fungi and less gram-positive bacteria are unsuitable for the high-pressure homogenization method because of more easily causing valve blockage.

The ball mill that Switzerland China precious grinder Manufacturing Co., Ltd produces is the horizontal ball mill that has agitator, clasmatosis mechanism is that the interior high-speed rotating impeller of cylinder body is with cell suspending liquid and micro glass beads strong agitation, by means of strong shearing between bead and the cell and collision effect, cause cell rupture.This machine can be used for the cell of disruption of microorganisms and biogenetic products, is the double mechanical seal structure but its adopts, and tiny bead and the glass chip that is broken, wearing and tearing micro mist can enter the sealing of spindles place when spindle operation.Because of glass hard (HRC65 is very high, can cause a closure material heavy wear, influence sealing effectiveness and life-span.This machine adopts the pearl liquid separating component of high speed rotating also can not definitely stop the wearing and tearing micro mist to enter an envelope place.

The purpose of this utility model is to improve hermetically-sealed construction, prevents that the glass chip under the high speed from entering the main shaft seal place, avoids main shaft and the wearing and tearing of axle closure material, prolongs the service life of biomixer.

In order to realize this purpose, the utility model adopts non-metal knitted filler, and the distribution ring of band cooling and flushing is arranged in filler, on this distribution ring, pressure pipeline is housed, cool off and wash with middle water filling, avoided main shaft and the wearing and tearing of axle closure material, guaranteed the normal operation of biomixer.

Below in conjunction with detailed description and the description of the drawings thereof of implementing embodiment of the present utility model, structure composition of the present utility model, operation principle and advantage thereof are done a detailed introduction.

Accompanying drawing of the present utility model is

Fig. 1 is the structural representation of present embodiment.

Fig. 2 is the schematic diagram that distributes ring.

As shown in Figure 1 and Figure 2, the biomixer structure shown in the present embodiment is as follows: biomixer mainly is made up of motor [1], cylinder body [17], main shaft [4], impeller [10], charging aperture [13], discharging opening [16], exhaust outlet [9], potted component [18], grinding agent [19], axle sleeve [5], distribution ring [6], pressure channel [7], cooling jacket [20].

The internal diameter Φ 154mm of cylinder body [17], charging aperture [13] is arranged at its end cap top, and filter screen [15] and the discharging opening [16] of holding back grinding agent [19] usefulness is equipped with in the bottom.Exhaust outlet [9] is arranged at the cylinder body top, but when filling with substance emptying.Impeller [10] has 5, equidistant being installed on the main shaft [4], and impeller [10] is made by polyurethane material, polyurethane plastics is wear-resisting especially, avirulence, but machined again, so be good impeller material, hardness should be advisable with Shao Shi A90～95, and this material is better to the function of smudge cells.Impeller [10] also can be made by stainless steel, and this material impeller is better to the material dispersed function.Cylinder body [17] is outer to be with cooling jacket [20], but interior water flowing or freezing liquid, cooling water outlet and inlet [11], in order to the inner slip of cooling cylinder body [17].

Between main shaft [4] and potted component [18] axle sleeve [5] is arranged, axle sleeve [5] and main shaft [4] are fixed by key.Potted component [18] is the annular non-metal knitted filler that is contained on the axle sleeve [5], can use the polytetrafluoroethylene (PTFE) braided packing, also can use carbon fibre braided packing.

In the non-metal knitted filler of annular, be set with the distribution ring [6] of band cooling and flushing, distribute on the ring [6] to have two grooves [21], [22], have some apertures [23] to communicate between two grooves [21] [22].On distribution ring [6], pressure pipeline [7] is housed, logical middle water filling in pressure pipeline [7], pressure is 0.1～0.15MPa.Measure by Pressure gauge [24].

The grinding agent [19] that adds in cylinder body [17] is 0.5～0.8mm crown glass pearl 3.8l, and load factor is 0.8.Also can in cylinder body, add zirconium oxide bead as grinding agent.Add Blakeslea trispora mycelium suspension from charging aperture [13], suspension concentration is 4.5-10%.

Open middle water filling earlier, middle water filling in pressure pipeline [7] from bottom to top, by valve [8] controlled pressure, in order to the filler of cooling axle envelope.Start motor [1] again, drive main shaft [4] by belt [2], belt pulley [3], bearing [25], the rotating speed of main shaft [4] is 2870r/min.With being loaded on the temperature that bimetallic thermometer on the cylinder end caps records cylinder body inner cell suspension is about 30 ℃, is measured by thermometer [14].Cylinder interior pressure is measured by Pressure gauge [12].Under high speed rotating, tiny bead and the glass chip that is broken might enter main shaft shaft packing place.At this moment with the middle water filling of band pressure braided packing is cooled off, the middle water filling that has pressure simultaneously can be got back to the glass chip top in the cylinder body [17], plays flushing action.Avoided main shaft [4] and axle sleeve [5] to damage because of the grinding that is subjected to the hard glass chip.Middle water filling can be a running water, also can be sterilized water or the distilled water that recycles.

The clasmatosis crusher machine that adopts present embodiment after 4 minutes the Blakeslea trispora mycelium interrupted, fragmentation, orange-yellow cell inclusion is released.The performance of smudge cells reaches external advanced level.Noise during clasmatosis is less than the national regulation value.

Present embodiment is owing to there is the distribution ring of band cooling and flushing in non-metal knitted filler, on the distribution ring, pressure pipeline is arranged, in the pressure pipeline logical in water filling, the high speed rotating seal member of Zu Chenging like this, the wearing and tearing micro mist that can block after grinding with pressure effectively enters the rotary seal parts.And the assembly of double mechanical seal that can be complicated.These architectural features are main special cards of the present utility model.

The utility model is owing to adopted the distribution ring that has cooling and flushing in the braided packing shown in the above-mentioned principal character, on the distribution ring, pressure pipeline is housed, logical middle water filling in the pressure pipeline, grinding agent adopts the crown glass pearl, just can make biomixer of the present invention solve the sealing reliability of clasmatosis equipment, improve service life, reduced equipment cost, become new and effective biomixer.

The utlity model has simple in structure, easy to process, block wearing and tearing with pressure Micro mist enters the rotary seal parts, thus reach that sealing is reliable, long service life, The advantage that equipment cost is low has guaranteed that new and effective biomixer is continuously smooth Running has overcome the deficiencies in the prior art part.

Claims (3)

1, a kind of biomixer, comprise motor [1], cylinder body [17], main shaft [4], impeller [10], charging aperture [13], discharging opening [16], grinding agent [19], potted component [18], cooling jacket [20], it is characterized in that: described potted component [18] is the annular non-metal knitted filler that is contained on the axle sleeve [5], in the non-metal knitted filler of some annulars, be set with the distribution ring [6] of band cooling and flushing, on this distribution ring [6] pressure pipeline [7] is housed, described grinding agent [19] is a kind of in crown glass pearl or the zirconium oxide bead.

2, according to the broken machine of the described cell glass of claim 1, it is characterized in that: the non-metal knitted filler of described annular is to be made by a kind of material in polytetrafluoroethylene (PTFE) braided packing, the carbon fibre braided packing.

3, according to the described biomixer of claim 1, it is characterized in that: have two grooves [21], [22] on the described distribution ring [6], have some apertures [23] to communicate between two grooves [21] [22].

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