CN221554522U - Vitrification freezing and storing perfusion device - Google Patents
Vitrification freezing and storing perfusion device Download PDFInfo
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- CN221554522U CN221554522U CN202323287660.6U CN202323287660U CN221554522U CN 221554522 U CN221554522 U CN 221554522U CN 202323287660 U CN202323287660 U CN 202323287660U CN 221554522 U CN221554522 U CN 221554522U
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- 238000004017 vitrification Methods 0.000 title claims abstract description 33
- 230000010412 perfusion Effects 0.000 title claims abstract description 27
- 238000007710 freezing Methods 0.000 title abstract description 8
- 230000008014 freezing Effects 0.000 title abstract description 8
- 238000000926 separation method Methods 0.000 claims abstract description 14
- 238000005138 cryopreservation Methods 0.000 claims description 33
- 239000004065 semiconductor Substances 0.000 claims description 6
- 238000005057 refrigeration Methods 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 36
- 238000002347 injection Methods 0.000 description 9
- 239000007924 injection Substances 0.000 description 9
- 238000001816 cooling Methods 0.000 description 7
- 239000000872 buffer Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 239000007853 buffer solution Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 125000006850 spacer group Chemical group 0.000 description 4
- 238000001802 infusion Methods 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 229910001220 stainless steel Inorganic materials 0.000 description 3
- 239000010935 stainless steel Substances 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000012620 biological material Substances 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000012536 storage buffer Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The utility model discloses a vitrification freezing and storing perfusion device, which comprises a box body, wherein the top wall of the box body is provided with a first through hole and at least two second through holes; a taking and placing space is arranged below the first through hole, a separation net is arranged on the outer side of the taking and placing space, and the separation net is connected with the box body. By arranging the first through hole, the cell filter screen is convenient to take and put; by arranging two second through holes, the liquid can be conveniently added and recovered; the first through hole and the second through hole are arranged on the top wall of the box body, so that liquid cannot overflow during perfusion; through setting up the screen, the position of restriction cell filter screen prevents that the cell filter screen from dropping to the box body and getting the region outside the space of putting, is convenient for follow-up take out the cell filter screen.
Description
Technical Field
The utility model relates to the field of biological material preservation, in particular to a vitrification cryopreservation perfusion device.
Background
Vitrification cryopreservation is a common method of preserving tissues or cells. Vitrification is a process that uses high concentrations of cryoprotectants to induce the transformation of tissue or cells into a glassy solid state and rapidly cools at-196 ℃. In the process of converting tissues or cells into a glassy solid state, the tissues or cells need to be repeatedly soaked in different types of liquids, and the conventional freezing storage device is inconvenient for adding and recovering various liquids.
Disclosure of utility model
The present utility model aims to solve at least one of the technical problems existing in the prior art. Therefore, the utility model provides a vitrification cryopreservation perfusion device.
The vitrification freezing and storing perfusion device comprises a box body, wherein a first through hole and at least two second through holes are formed in the top wall of the box body; the lower part of the first through hole is provided with a picking and placing space, the outer side of the picking and placing space is provided with a separation net, and the separation net is connected with the box body.
The vitrification freezing and storing perfusion device provided by the embodiment of the utility model has at least the following technical effects: when the vitrification frozen stock solution is used, tissues to be frozen can be placed on the cell filter screen, then the cell filter screen is placed in the taking and placing space, and then a liquid injection pipe is inserted into one of the second through holes so as to inject vitrification frozen stock buffer solution; after soaking for a period of time, respectively inserting a liquid injection pipe and a liquid suction pipe into the two second through holes to inject vitrified cryopreservation liquid and draw out vitrified cryopreservation buffer liquid, repeating the process of filling the vitrified cryopreservation buffer liquid for two to three times, gradually replacing the vitrified cryopreservation buffer liquid with vitrified cryopreservation liquid, quantitatively subpackaging the tissues into the cryopreservation pipes, adding the vitrified cryopreservation liquid, and rapidly cooling and cryopreserving at the temperature of minus 196 ℃. By arranging the first through hole, the cell filter screen is convenient to take and put; by arranging two second through holes, the liquid can be conveniently added and recovered; the first through hole and the second through hole are arranged on the top wall of the box body, so that liquid cannot overflow during perfusion; through setting up the screen, the position of restriction cell filter screen prevents that the cell filter screen from dropping to the box body and getting the region outside the space of putting, is convenient for follow-up take out the cell filter screen.
According to some embodiments of the utility model, the vitrification freezing and storing perfusion device further comprises a base, wherein a refrigerating piece is arranged in the base, a groove is arranged on the top surface of the base, and the bottom of the box body is arranged in the groove. When the refrigerator is used, the refrigerating piece can be started to cool the box body, so that the box body is kept at a lower temperature, the metabolism of tissues is reduced, and the preservation of the tissues is facilitated.
According to some embodiments of the utility model, the cooling element is a semiconductor cooling fin. Thus, the structure is simple and the setting is simple.
According to some embodiments of the utility model, a first cover body capable of being opened and closed is arranged on the upper side of the box body, and the first cover body is covered on the upper side of the first through hole. After the tissue is put into the box body, the first cover body can be closed, so that the external heat is reduced from being transmitted into the box body, and the box body is kept at a lower temperature.
According to some embodiments of the utility model, the first cover is hinged to the case. The structure is simple, and the first cover body can be conveniently opened and closed.
According to some embodiments of the utility model, the first cover covers all the second through holes, a third through hole is arranged above each first cover corresponding to each second through hole, and a second cover capable of being opened and closed is arranged on an upper side cover of each third through hole. The second cover body can be closed when the tissue is soaked, and the second cover body is opened when perfusion is needed to connect the liquid injection pipe and the liquid suction pipe, so that external heat is further reduced and transferred into the box body.
According to some embodiments of the utility model, the second cover is hinged to the first cover. Thus, the structure is simple, and the second cover body is convenient to open and close.
According to some embodiments of the utility model, the upper side of the second cover is provided with a handle. Thus, the operator can conveniently open and close the second cover body.
According to some embodiments of the utility model, the number of second through holes is three. Therefore, the two independent liquid injection pipes can be used for injecting vitrification frozen storage buffer solution and vitrification frozen storage solution respectively, and the operation is more convenient.
According to some embodiments of the utility model, the top of the spacer is detachably connected to the edge of the first through hole. Therefore, the separation net can be directly taken out when the tissue needs to be taken out, and the operation is more convenient.
Additional aspects and advantages of the utility model will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the utility model.
Drawings
The foregoing and/or additional aspects and advantages of the utility model will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:
Fig. 1 is a schematic perspective view of a vitrification cryopreservation perfusion apparatus according to an embodiment of the present utility model.
Fig. 2 is a right side cross-sectional schematic view of fig. 1.
Fig. 3 is a schematic perspective view of the case in fig. 1.
In the accompanying drawings:
100-box body; 101-a first through hole; 102-a second via; 110-a screen; 111-cell strainer; 120-a first hinge shaft; 200-base; 210-semiconductor refrigerating sheets; 220-stainless steel sheet; 300-a first cover; 301-a third through hole; 310-a second cover; 311-handle; 312-second hinge shaft.
Detailed Description
Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the utility model.
In the description of the present utility model, it should be understood that references to orientation descriptions such as upper, lower, front, rear, left, right, etc. are based on the orientation or positional relationship shown in the drawings, only for convenience of description and simplification of the description, and are not intended to indicate that the apparatus or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Further, the meaning of a plurality is one or more, and the meaning of a plurality is two or more, and greater than, less than, exceeding, etc. is understood to exclude the present number, and the meaning of above, below, within, etc. is understood to include the present number. The description of the first and second is for the purpose of distinguishing between technical features only and should not be construed as indicating relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.
In the description of the present utility model, unless explicitly defined otherwise, terms such as arrangement, installation, connection, etc. should be construed broadly and the specific meaning of the terms in the present utility model can be reasonably determined by a person skilled in the art in combination with the specific contents of the technical scheme.
A vitrification cryopreservation perfusion apparatus according to an embodiment of the present utility model is described below with reference to fig. 1 to 3.
The vitrification freezing and storing perfusion device comprises a box body 100, wherein a first through hole 101 and at least two second through holes 102 are formed in the top wall of the box body 100; a picking and placing space is arranged below the first through hole 101, a separation net 110 is arranged on the outer side of the picking and placing space, and the separation net 110 is connected with the box body 100.
For example, as shown in fig. 3, the case 100 has a rectangular parallelepiped shape, the first through hole 101 is disposed in the center of the top wall of the case 100, and the plurality of second through holes 102 are sequentially disposed along the circumferential direction of the first through hole 101; the taking and placing space is arranged in the box body 100, the taking and placing space is in a column shape which is vertically arranged, the separation net 110 comprises four first connecting strips which are vertically arranged and two second connecting strips which are horizontally arranged, the two second connecting strips are crossed, the two second connecting strips are positioned at the lower side of the taking and placing space, the four first connecting strips are respectively arranged at the front side, the rear side, the left side and the right side of the taking and placing space, and two end parts of each second connecting strip are connected with the lower end of one first connecting strip; of course, the screen 110 may have other suitable structures for supporting the cell screen 111 in the picking and placing space.
When in use, tissues to be frozen can be placed on the cell filter screen 111, then the cell filter screen 111 is placed in the taking and placing space, and then a liquid injection pipe is inserted into one of the second through holes 102 to inject vitrification frozen buffer solution; after soaking for a period of time, the liquid injection pipe and the liquid extraction pipe are respectively inserted into the two second through holes 102 to inject vitrified cryopreservation liquid and extract vitrified cryopreservation buffer liquid, the process of filling is repeated for two to three times, the vitrified cryopreservation buffer liquid is gradually replaced by vitrified cryopreservation liquid, and then tissues are quantitatively packaged into the cryopreservation pipe, the vitrified cryopreservation liquid is added, and the frozen tissues are rapidly cooled and stored at the temperature of minus 196 ℃. By providing the first through hole 101, the cell filter 111 is conveniently taken and put; by providing two second through holes 102, liquid is convenient to add and recover; by arranging the first through hole 101 and the second through hole 102 on the top wall of the box body 100, liquid cannot overflow during perfusion; by arranging the separation net 110, the position of the cell filter 111 is limited, so that the cell filter 111 is prevented from falling to an area outside the taking-out and placing space in the box body 100, and the cell filter 111 can be taken out conveniently. The liquid injection pipe and the liquid suction pipe can be needle heads of the infusion apparatus, and when the infusion apparatus is used, the liquid in the syringe can be injected into the box body 100 or the liquid in the box body 100 can be pumped into the syringe by connecting the syringe with the infusion apparatus. The utility model is also applicable to resuscitation of frozen tissues.
In some embodiments of the present utility model, the vitrification cryopreservation perfusion apparatus further includes a base 200, a refrigerating element is disposed in the base 200, a groove is disposed on the top surface of the base 200, and the bottom of the box body 100 is disposed in the groove. When in use, the refrigerating piece can be started to cool the box body 100, so that the box body 100 is kept at a lower temperature, the metabolism of tissues is reduced, and the preservation of the tissues is facilitated.
In some embodiments of the utility model, the cooling element is a semiconductor cooling fin 210. Referring to fig. 2, the bottom wall of the groove is a stainless steel sheet 220, and the semiconductor cooling sheet 210 is stacked on the lower side of the stainless steel sheet 220, and the lower side of the semiconductor cooling sheet 210 is opened to facilitate heat dissipation. Thus, the structure is simple and the setting is simple.
In some embodiments of the present utility model, referring to fig. 1, a first cover 300 is provided on an upper side of the case 100 to be openable and closable, and the first cover 300 is provided on an upper side of the first through hole 101. After the tissue is placed into the box body 100, the first cover body 300 can be closed, so that external heat is reduced from being transferred into the box body 100, and the box body 100 is kept at a lower temperature.
In some embodiments of the present utility model, the first cover 300 is hinged with the case 100. The structure is simple, and the first cover 300 can be conveniently opened and closed; wherein, a clamping structure or other suitable structure can be arranged between the first cover 300 and the box 100 for detachable connection. The hinge shaft at the hinge of the first cover 300 and the case 100 is referred to as a first hinge shaft 120, the first hinge shaft 120 is disposed at the rear edge of the first cover 300, and the first hinge shaft 120 is disposed laterally.
In some embodiments of the present utility model, the first cover 300 is disposed on all the second through holes 102, a third through hole 301 is disposed above each of the first through holes 300 corresponding to each of the second through holes 102, and an openable second cover 310 is disposed on an upper side of each of the third through holes 301. When the tissue is soaked, the second cover body 310 can be closed, and when the perfusion is needed, the second cover body 310 is opened to connect the liquid injection pipe and the liquid suction pipe, so that the external heat is further reduced to be transmitted into the box body 100.
In some embodiments of the present utility model, the second cover 310 is hinged with the first cover 300. This is simple in structure, and facilitates the opening and closing of the second cover 310. The hinge shaft at the hinge of the second cover 310 and the first cover 300 is referred to as a second hinge shaft 312, and the second hinge shaft 312 is vertically disposed.
In some embodiments of the present utility model, the upper side of the second cover 310 is provided with a handle 311. This facilitates the operator to operate the opening and closing of the second cover 310.
In some embodiments of the present utility model, the number of second vias 102 is three. Such three second through holes 102 can be used for connecting the vitrification cryopreservation buffer solution pipeline, the vitrification cryopreservation solution pipeline and the waste liquid pipeline respectively, and two independent liquid injection pipes can be used for injecting the vitrification cryopreservation buffer solution and the vitrification cryopreservation solution respectively, so that the operation is more convenient.
In some embodiments of the present utility model, the top of the spacer 110 is removably connected to the edge of the first through hole 101. The top of the separation net 110 can be provided with an annular convex edge, so that the annular convex edge is arranged on the upper side of the edge of the first through hole 101, and the separation net 110 can be detachably connected with the first through hole 101; of course, the removable connection of the spacer 110 to the edge of the first through hole 101 may be achieved by a snap fit, a threaded connection, or other suitable means. This allows for direct removal of the spacer mesh 110 for easier handling when tissue removal is desired.
While the preferred embodiment of the present utility model has been illustrated and described, the present utility model is not limited to the embodiments, and various equivalent modifications and substitutions can be made by those skilled in the art without departing from the spirit of the present utility model, and these equivalent modifications and substitutions are intended to be included in the scope of the present utility model as defined in the appended claims.
Claims (10)
1. The vitrification cryopreservation perfusion device is characterized in that: the box comprises a box body, wherein a first through hole and at least two second through holes are formed in the top wall of the box body; the lower part of the first through hole is provided with a picking and placing space, the outer side of the picking and placing space is provided with a separation net, and the separation net is connected with the box body.
2. The vitrification cryopreservation perfusion apparatus of claim 1, wherein: still include the base, be equipped with the refrigeration piece in the base, the top surface of base is equipped with the recess, the bottom of box body is located in the recess.
3. The vitrification cryopreservation perfusion apparatus of claim 2, wherein: the refrigerating piece is a semiconductor refrigerating piece.
4. The vitrification cryopreservation perfusion apparatus of claim 2, wherein: the upper side of box body is equipped with the first lid that can open and shut, first lid is located the upside of first through-hole.
5. The vitrification cryopreservation perfusion apparatus of claim 4, wherein: the first cover body is hinged with the box body.
6. The vitrification cryopreservation perfusion apparatus of claim 4, wherein: the first cover body covers all the second through holes, third through holes are formed in the first cover body corresponding to the upper sides of the second through holes, and second cover bodies capable of being opened and closed are arranged on upper side covers of the third through holes.
7. The vitrification cryopreservation perfusion apparatus of claim 6, wherein: the second cover body is hinged with the first cover body.
8. The vitrification cryopreservation perfusion apparatus of claim 6, wherein: the upper side of the second cover body is provided with a handle.
9. The vitrification cryopreservation perfusion apparatus of claim 1, wherein: the number of the second through holes is three.
10. The vitrification cryopreservation perfusion apparatus of claim 1, wherein: the top of the separation net is detachably connected with the edge of the first through hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323287660.6U CN221554522U (en) | 2023-12-01 | 2023-12-01 | Vitrification freezing and storing perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323287660.6U CN221554522U (en) | 2023-12-01 | 2023-12-01 | Vitrification freezing and storing perfusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221554522U true CN221554522U (en) | 2024-08-20 |
Family
ID=92293818
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202323287660.6U Active CN221554522U (en) | 2023-12-01 | 2023-12-01 | Vitrification freezing and storing perfusion device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221554522U (en) |
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2023
- 2023-12-01 CN CN202323287660.6U patent/CN221554522U/en active Active
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