CN221296907U - Real-time cell culture solution providing device - Google Patents
Real-time cell culture solution providing device Download PDFInfo
- Publication number
- CN221296907U CN221296907U CN202322820157.6U CN202322820157U CN221296907U CN 221296907 U CN221296907 U CN 221296907U CN 202322820157 U CN202322820157 U CN 202322820157U CN 221296907 U CN221296907 U CN 221296907U
- Authority
- CN
- China
- Prior art keywords
- temperature storage
- cylindrical sealing
- culture solution
- cell culture
- low temperature
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000004113 cell culture Methods 0.000 title claims abstract description 15
- 235000015097 nutrients Nutrition 0.000 claims abstract description 28
- 238000002156 mixing Methods 0.000 claims abstract description 20
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052802 copper Inorganic materials 0.000 claims abstract description 18
- 239000010949 copper Substances 0.000 claims abstract description 18
- 239000000654 additive Substances 0.000 claims abstract description 15
- 230000000996 additive effect Effects 0.000 claims abstract description 15
- 238000007789 sealing Methods 0.000 claims description 41
- 239000007788 liquid Substances 0.000 claims description 7
- 239000012930 cell culture fluid Substances 0.000 claims description 4
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 abstract description 5
- 210000002966 serum Anatomy 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000003115 biocidal effect Effects 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 229940088710 antibiotic agent Drugs 0.000 description 4
- 230000000694 effects Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000428 dust Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a cell culture solution real-time supply device which comprises a culture solution low-temperature storage tank, wherein a low-temperature storage sleeve is arranged on the outer side of the culture solution low-temperature storage tank, a nutrient solution discharge valve is fixed on a lower bolt of the culture solution low-temperature storage tank, a nutrient solution mixing tank is arranged on the right side of the lower part of the culture solution low-temperature storage tank, and a supply electric control valve is arranged on a lower bolt of the nutrient solution mixing tank. The beneficial effects of the utility model are as follows: through the setting of cylinder seal shell, servo motor, fixed end cover, sealed arch and fixed conductive copper sheet, be favorable to controlling the external feed pump power supply when connecting the additive pipeline, make serum, glutamine and antibiotic and base solution respectively intensive mixing be in the same place to the staff need not to control the external feed pump supply alone during the ratio.
Description
Technical Field
The utility model belongs to the technical field of real-time supply of cell culture fluid, and particularly relates to a real-time supply device of cell culture fluid.
Background
The culture solution is used for carrying out the collective term of the medium of tissue or cell culture, and the culture solution needs to be used after mixing with serum, glutamine and antibiotics etc. before using, and current cell culture solution provides the device in real time, including cell culture seat, culture tank, nutrient solution groove, support threaded rod, U type lift pole, observable real-time bottle structure that supports, rotatable level detects seat structure, adjustable support fixing base structure, dust cover, first sealing strip, second sealing strip, locking through-hole, rubber circle and wing nut, the culture tank from left to right offer in proper order in the upper end of cell culture seat, this kind of equipment needs the staff to add frequently in the use to the interpolation in-process still need to control the additive amount, the step is loaded down with trivial details in the interpolation process, and efficiency is lower.
Disclosure of utility model
Aiming at the technical problems, the utility model provides a cell culture solution real-time providing device, which is used for storing culture solution base solution, serum, glutamine and antibiotics separately, automatically controlling additive amount by utilizing an electric control valve and rapidly mixing the additive amount with a mixing structure.
The technical scheme is as follows: the utility model provides a cell culture solution provides device in real time, includes culture solution low temperature storage jar, the outside of culture solution low temperature storage jar install the low temperature storage cover, the lower part bolt fastening of culture solution low temperature storage jar has the nutrient solution discharge valve, the lower part right side of culture solution low temperature storage jar is provided with the nutrient solution blending tank, the lower part bolt installation of nutrient solution blending tank supplies with automatically controlled valve, its characterized in that, the right side lower part of low temperature storage cover install outside feed pump cooperation controller, the rotation control case is installed to the inboard of outside feed pump cooperation controller, the upper portion of outside feed pump cooperation controller is provided with the additive pipeline.
Preferably, the external supply pump cooperation controller comprises a cylindrical sealing shell, a servo motor is fixed at the rear end of the cylindrical sealing shell through bolts, a fixed end cover is mounted at the front end of the cylindrical sealing shell through bolts, a sealing bulge is integrally arranged at the rear end of the fixed end cover, and a fixed conductive copper sheet is embedded in the upper portion of the rear end of the sealing bulge.
Preferably, the rotary control valve core comprises a cylindrical sealing valve core, the front end and the rear end of the cylindrical sealing valve core are respectively connected with a driving shaft rod in a key way, a through guide hole is formed in the middle position of the inner side of the cylindrical sealing valve core, and a conductive trigger copper sheet is inlaid in the middle position of the upper part of the front end of the cylindrical sealing valve core.
Preferably, the cylindrical sealing shell is fixed on the lower right side of the low-temperature storage sleeve by bolts, and the lower part of the cylindrical sealing shell is communicated with the upper right part of the nutrient solution mixing tank by a pipeline.
Preferably, a plurality of fixed conductive copper sheets are arranged, two fixed conductive copper sheets are respectively arranged in a group, and each group of fixed conductive copper sheets is positioned at the lower part of the additive pipeline.
Preferably, the additive pipelines are respectively welded at the left side and the right side of the upper part of the cylindrical sealing shell and at the middle position of the upper part of the cylindrical sealing shell.
Compared with the prior art, the utility model has the beneficial effects that:
1. In the utility model, the arrangement of the cylindrical sealing shell, the servo motor, the fixed end cover, the sealing bulge and the fixed conductive copper sheet is beneficial to controlling the power supply of the external supply pump while communicating the additive pipeline, so that serum, glutamine, antibiotics and base liquid are respectively and fully mixed together, and no staff is required to independently control the supply of the external supply pump during proportioning.
2. According to the utility model, the cylindrical sealing valve core, the driving shaft lever, the through guide hole and the conductive trigger copper sheet are arranged, so that independent communication of a plurality of arranged additive pipelines is facilitated, and linkage control of an external supply pump is realized by matching with the fixed conductive copper sheet.
Drawings
Fig. 1 is a schematic structural view of the present utility model.
Fig. 2 is a rear view of the external feed pump engagement controller of the present utility model.
Fig. 3 is a schematic structural view of the rotary control valve cartridge of the present utility model.
In the figure:
1. A low-temperature storage tank for the culture solution; 2. a low-temperature storage sleeve; 3. a nutrient solution discharge valve; 4. a nutrient solution mixing tank; 5. supplying an electric control valve; 6. an external feed pump is matched with the controller; 61. a cylindrical seal housing; 62. a servo motor; 63. fixing the end cover; 64. a sealing protrusion; 65. fixing a conductive copper sheet; 7. rotating the control valve core; 71. a cylindrical sealing valve core; 72. a drive shaft; 73. a through guide hole; 74. a conductive trigger copper sheet; 8. additive pipes.
Detailed Description
The utility model is further described below with reference to the accompanying drawings:
Examples:
As shown in fig. 1, a cell culture solution real-time providing device comprises a culture solution low-temperature storage tank 1, wherein a low-temperature storage sleeve 2 is installed on the outer side of the culture solution low-temperature storage tank 1, a nutrient solution discharge valve 3 is fixed on the lower portion bolt of the culture solution low-temperature storage tank 1, a nutrient solution mixing tank 4 is arranged on the right side of the lower portion of the culture solution low-temperature storage tank 1, a supply electric control valve 5 is installed on the lower portion bolt of the nutrient solution mixing tank 4, and the cell culture solution real-time providing device is characterized in that an external supply pump matching controller 6 is installed on the lower portion of the right side of the low-temperature storage sleeve 2, a rotary control valve core 7 is installed on the inner side of the external supply pump matching controller 6, and an additive pipeline 8 is arranged on the upper portion of the external supply pump matching controller 6.
In the above embodiment, specifically, the lower part of the nutrient solution discharge valve 3 is communicated with the upper left part of the nutrient solution mixing pot 4 through a pipe.
In the above embodiment, specifically, the cylindrical sealing spool 71 is placed inside the cylindrical sealing shell 61, and the outer side of the cylindrical sealing spool 71 is glued with a rubber gasket.
In the above embodiment, specifically, the driving shaft 72 is journaled inside the cylindrical sealing case 61, and the rear end of the driving shaft 72 is coupled with the servo motor 62.
Principle of operation
The working principle of the utility model is as follows: the low-temperature medium enters the inner side of the low-temperature storage sleeve 2 to store the medium liquid in the inner side of the medium low-temperature storage tank 1 at a low temperature, the opening time of the nutrient solution discharge valve 3 is controlled by the external controller, the medium liquid in the inner side of the medium low-temperature storage tank 1 is quantitatively discharged to the inner side of the nutrient solution mixing tank 4, the servo motor 62 is controlled by the external controller to electrify, the servo motor 62 drives the cylindrical sealing valve core 71 to rotate in the inner side of the cylindrical sealing shell 61 through the driving shaft rod 72, different additive pipelines 8 at the upper part of the cylindrical sealing shell 61 are communicated through the orientation of the through guide hole 73, the communicating time of the inner side of the cylindrical sealing valve core 71 is controlled by the servo motor 62, the conductive triggering copper sheet 74 contacts with the fixed conductive copper sheet 65 at a proper position to play a role of a switch, the communicating of the external supply pump is controlled, the external supply pump is enabled to enable external serum, glutamine and antibiotics to enter the inner side of the nutrient solution mixing tank 4 respectively, the rotating motor at the upper part of the nutrient solution mixing tank drives the inner side to rotate, the mixing stirring rod is fully mixed, finally, the supply electric control valve 5 is opened, the nutrient solution is supplied to the proper position, and the nutrient solution can be separated from the nutrient solution before being supplied to the nutrient solution and the nutrient solution can be stored.
By using the technical scheme of the utility model or under the inspired by the technical scheme of the utility model, a similar technical scheme is designed by a person skilled in the art, so that the technical effects are achieved, and the technical effects fall into the protection scope of the utility model.
Claims (6)
1. The utility model provides a cell culture solution provides device in real time, includes culture solution low temperature storage jar (1), the outside of culture solution low temperature storage jar (1) install low temperature storage cover (2), the lower part bolt fastening of culture solution low temperature storage jar (1) has nutrient solution discharge valve (3), the lower part right side of culture solution low temperature storage jar (1) is provided with nutrient solution blending tank (4), feed electric control valve (5) are installed to the lower part bolt of nutrient solution blending tank (4), a serial communication port, the right side lower part of low temperature storage cover (2) install outside feed pump cooperation controller (6), rotation control case (7) are installed to the inboard of outside feed pump cooperation controller (6), the upper portion of outside feed pump cooperation controller (6) is provided with additive pipeline (8).
2. The cell culture fluid real-time supply device according to claim 1, wherein the external supply pump cooperation controller (6) comprises a cylindrical sealing shell (61), a servo motor (62) is fixed at the rear end of the cylindrical sealing shell (61) through bolts, a fixed end cover (63) is mounted at the front end of the cylindrical sealing shell (61) through bolts, a sealing bulge (64) is integrally arranged at the rear end of the fixed end cover (63), and a fixed conductive copper sheet (65) is embedded at the upper part of the rear end of the sealing bulge (64).
3. The cell culture fluid real-time supply device according to claim 1, wherein the rotary control valve core (7) comprises a cylindrical sealing valve core (71), the front end and the rear end of the cylindrical sealing valve core (71) are respectively connected with a driving shaft lever (72) in a key way, a through guide hole (73) is formed in the middle position of the inner side of the cylindrical sealing valve core (71), and a conductive trigger copper sheet (74) is inlaid in the middle position of the upper part of the front end of the cylindrical sealing valve core (71).
4. The cell culture liquid real-time supply device according to claim 2, wherein the cylindrical sealing case (61) is bolted to the right lower part of the low temperature storage jacket (2), and the lower part of the cylindrical sealing case (61) is communicated with the right upper part of the nutrient liquid mixing tank (4) through a pipe.
5. The cell culture liquid real-time supply device according to claim 2, wherein a plurality of fixed conductive copper sheets (65) are provided, and the fixed conductive copper sheets (65) are respectively arranged in a group, and each group of fixed conductive copper sheets (65) is arranged at the lower part of the additive pipeline (8).
6. The apparatus for supplying a cell culture liquid according to claim 2, wherein the additive pipe (8) is welded to both left and right sides of the upper portion of the cylindrical sealing case (61) and to an upper intermediate position of the cylindrical sealing case (61), respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322820157.6U CN221296907U (en) | 2023-10-20 | 2023-10-20 | Real-time cell culture solution providing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322820157.6U CN221296907U (en) | 2023-10-20 | 2023-10-20 | Real-time cell culture solution providing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221296907U true CN221296907U (en) | 2024-07-09 |
Family
ID=91733991
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322820157.6U Active CN221296907U (en) | 2023-10-20 | 2023-10-20 | Real-time cell culture solution providing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221296907U (en) |
-
2023
- 2023-10-20 CN CN202322820157.6U patent/CN221296907U/en active Active
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