CN221203871U - Regulatory T cell preservation device - Google Patents
Regulatory T cell preservation device Download PDFInfo
- Publication number
- CN221203871U CN221203871U CN202322686816.1U CN202322686816U CN221203871U CN 221203871 U CN221203871 U CN 221203871U CN 202322686816 U CN202322686816 U CN 202322686816U CN 221203871 U CN221203871 U CN 221203871U
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- China
- Prior art keywords
- fixedly connected
- shell
- cell preservation
- regulatory
- liquid nitrogen
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- 238000004321 preservation Methods 0.000 title claims abstract description 24
- 210000003289 regulatory T cell Anatomy 0.000 title claims description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 56
- 230000007246 mechanism Effects 0.000 claims abstract description 37
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 28
- 238000012360 testing method Methods 0.000 claims abstract description 25
- 229910000831 Steel Inorganic materials 0.000 claims description 8
- 239000010959 steel Substances 0.000 claims description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 239000000741 silica gel Substances 0.000 claims description 5
- 229910002027 silica gel Inorganic materials 0.000 claims description 5
- 210000001744 T-lymphocyte Anatomy 0.000 abstract description 30
- 210000004027 cell Anatomy 0.000 abstract description 12
- 230000009471 action Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The utility model discloses an adjustable T cell preservation device, and particularly relates to the technical field of cell preservation. The adjustable T cell preservation device provided by the utility model has the advantages that the pushing mechanism, the rotating cover and the like are arranged, the influence on other T cells can be reduced when the T cells are accessed, the rotating mechanism and the driven mechanism are arranged, the preserved T cells can slowly rotate in the device, the T cells in different test tubes can be fully contacted with liquid nitrogen under adjustment, the success rate of T cell preservation is ensured, and the practicability of the device is improved.
Description
Technical Field
The utility model relates to the technical field of cell preservation, in particular to a regulatory T cell preservation device.
Background
Cell cryopreservation is one of the main methods of cell preservation. The freezing technology is used to store the cells in liquid nitrogen at-196 deg.c to separate the cells from growth state temporarily and store their cell characteristics, so that the cells are recovered for experiment when needed. And a certain amount of cells are moderately preserved, so that the cells can be prevented from being lost due to pollution or other unexpected events of the cells being cultured, and the effect of preserving the cells is achieved.
Chinese patent document CN212464678U discloses an adjustable T cell preservation device, including box and low temperature protection cotton, the box upside is connected with the case lid, and box upper end mid-mounting has down the lock mounting panel, down lock mounting panel lower extreme is concavely inlayed and is had the fixed orifices, and down lock mounting panel mid-mounting has the tool to lock, tool to lock outside middle part swing joint has the ejector pad, and the ejector pad inboard is fixed with the tapered end. The adjustable T cell preservation device is provided with the box body, the fixedly connected box body and the lower lock mounting plate increase the mounting stability, so that the lower lock mounting plate can be used for a long time, and the preservation bottle stored in the interior is protected in a sealing way, so that the preservation bottle is not easily damaged by the outside, and the safety degree of the preservation bottle is ensured; but the following drawbacks also exist in the implementation:
When the above patent document stores T cells, if the T cells need to be taken out for use, the device can only be opened completely, and then the T cells can be taken out, which may affect other T cells in the device, and there is a problem that access is inconvenient.
Disclosure of utility model
The utility model mainly aims to provide a regulatory T cell preservation device which can effectively solve the problem of inconvenient access.
In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:
The utility model provides an adjustable T cell preservation device, includes the shell, shell upper end fixedly connected with slewing mechanism, inside swing joint of slewing mechanism has a plurality of test tubes, shell upper end screw thread connection has the rotation lid, four cushion of shell lower extreme fixedly connected with, two wall common fixedly connected with transparent plates about the shell inner chamber, shell left end fixedly connected with places the board, place board upper end swing joint has the liquid nitrogen bottle, liquid nitrogen bottle surface swing joint has fixed hoop, liquid nitrogen bottle upper end fixedly connected with valve, valve right-hand member fixedly connected with connecting pipe, shell right-hand member sliding connection pushing mechanism.
Preferably, the rotating mechanism comprises a fixed plate, the lower end of the fixed plate is fixedly connected with the upper end of the shell, the upper end of the fixed plate is fixedly connected with a motor, the output end of the motor is fixedly connected with a first bevel gear through a coupling, the outer surface of the first bevel gear is in meshed connection with a second bevel gear, the lower end of the second bevel gear is fixedly connected with a first rotating rod, the outer surface of the first rotating rod is fixedly connected with a rotating disc, and the lower end of the rotating rod is fixedly connected with a driven mechanism.
Preferably, the driven mechanism comprises a rotating rod II, two placing plates are fixedly connected to the outer surface of the rotating rod, a plurality of placing holes vertically penetrating through the placing plates are formed in the upper ends of the placing plates, a shaft core is fixedly connected to the lower end of the rotating rod II, a plurality of steel balls are movably connected to the outer surface of the shaft core, two outer shafts are movably connected to the outer surfaces of the steel balls, a shell is fixedly connected to the outer surfaces of the outer shafts, and the lower end of the shell is fixedly connected with the bottom wall of the inner cavity of the shell.
Preferably, the pushing mechanism comprises a push rod, the left end of the push rod is fixedly connected with a connecting sheet, the right end of the connecting sheet is fixedly connected with a spring, the left end of the connecting sheet is fixedly connected with a pushing block, and the lower end of the pushing block is slidably connected with a sliding groove block.
Preferably, the fixing hoop is provided with three arc plates, three the inner surface of each arc plate is fixedly connected with a silica gel pad, one end, close to each other, of each arc plate is fixedly connected with an arc telescopic rod, the outer surface of the arc plate positioned at the leftmost side of each arc plate is fixedly connected with a connector, and the right end of the connector is fixedly connected with the left end of the shell.
Preferably, the outer surfaces of the upper sides of the test tubes are fixedly connected with limiting sheets.
Compared with the prior art, the utility model has the following beneficial effects:
The T cell storage device is provided with the pushing mechanism, the rotating cover and the like, T cells can be taken out or stored under the condition of minimal influence on the inside of the device under the mutual cooperation, the influence on other T cells is reduced, the stored T cells can slowly rotate in the device due to the arrangement of the rotating mechanism and the driven mechanism, the T cells in different test tubes are covered by liquid nitrogen approximately the same under the adjustment, the success rate of T cell storage is ensured, and the practicability of the device is improved.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present utility model;
FIG. 2 is a schematic view of another view of the overall structure of the present utility model;
FIG. 3 is a schematic view of a rotating mechanism according to the present utility model;
FIG. 4 is a schematic view of a driven mechanism according to the present utility model;
FIG. 5 is a schematic view of a pushing mechanism according to the present utility model;
Fig. 6 is a schematic view of a fastening cuff of the present utility model.
In the figure: 1. a housing; 2. a pushing mechanism; 21. a push rod; 22. a connecting sheet; 23. a pushing block; 24. a spring; 25. a chute block; 3. a rotating mechanism; 31. a fixing plate; 32. a motor; 33. a first bevel gear; 34. a bevel gear II; 35. rotating the first rod; 36. a rotating disc; 37. a driven mechanism; 371. a second rotating rod; 372. placing a tray; 373. placing the hole; 374. a shaft core; 375. steel balls; 376. an outer collar; 377. a housing; 4. a rotary cover; 5. a test tube; 6. placing a plate; 7. a liquid nitrogen bottle; 8. a fixing hoop; 81. a joint; 82. an arc-shaped plate; 83. a silica gel pad; 84. an arc-shaped telescopic rod; 9. a valve; 10. a connecting pipe; 11. a transparent plate; 12. and (5) cushion blocks.
Detailed Description
The utility model is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the utility model easy to understand.
As shown in fig. 1-2, an adjustable T cell preservation device comprises a housing 1, wherein the upper end of the housing 1 is fixedly connected with a rotating mechanism 3, the rotating mechanism 3 can enable a plurality of test tubes 5 to rotate inside the device, the rotating mechanism 3 is movably connected with a plurality of test tubes 5 inside, the test tubes 5 are used for placing T cells, the upper end of the housing 1 is in threaded connection with a rotating cover 4, the rotating cover 4 is used for being matched with the access of the test tubes 5, the lower end of the housing 1 is fixedly connected with four cushion blocks 12, the upper wall and the lower wall of an inner cavity of the housing 1 are fixedly connected with transparent plates 11 together, and the transparent plates 11 are convenient for viewing the internal conditions of the device.
The shell 1 left end fixedly connected with places board 6, places board 6 and is used for placing liquid nitrogen bottle 7, places board 6 upper end swing joint and has liquid nitrogen bottle 7, and liquid nitrogen bottle 7 is used for providing liquid nitrogen and preserves T cell, and liquid nitrogen bottle 7 surface swing joint has fixed hoop 8, and the effect of fixed hoop 8 is fixed liquid nitrogen bottle 7, and liquid nitrogen bottle 7 upper end fixedly connected with valve 9, the output of valve 9 control liquid nitrogen, valve 9 right-hand member fixedly connected with connecting pipe 10, shell 1 right-hand member sliding connection has pushing mechanism 2, and pushing mechanism 2 is used for taking out test tube 5.
In order to improve the preservation success rate of the T cells, the T cells in different test tubes can be fully covered by liquid nitrogen at any time, referring to fig. 1 and 3-4, the rotating mechanism 3 comprises a fixed plate 31, the lower end of the fixed plate 31 is fixedly connected with the upper end of the shell 1, the upper end of the fixed plate 31 is fixedly connected with a motor 32, the output end of the motor 32 is fixedly connected with a first bevel gear 33 through a coupling, the outer surface of the first bevel gear 33 is in meshed connection with a second bevel gear 34, the lower end of the second bevel gear 34 is fixedly connected with a first rotating rod 35, the outer surface of the first rotating rod 35 is fixedly connected with a rotating disc 36, and the lower end of the first rotating rod 35 is fixedly connected with a driven mechanism 37.
The driven mechanism 37 comprises a second rotating rod 371, two placing discs 372 are fixedly connected to the outer surface of the second rotating rod 371, a plurality of placing holes 373 which vertically penetrate through the placing discs 372 are formed in the upper ends of the two placing discs 372, a shaft core 374 is fixedly connected to the lower end of the second rotating rod 371, a plurality of steel balls 375 are movably connected to the outer surface of the shaft core 374, two outer shafts 376 are movably connected to the outer surfaces of the plurality of steel balls 375, a shell 377 is fixedly connected to the outer surfaces of the two outer shafts 376 together, and the lower end of the shell 377 is fixedly connected with the bottom wall of an inner cavity of the shell 1.
The rotating cover 4 is screwed off, a plurality of test tubes 5 are filled in the holes to the plurality of placing holes 373, because the outer surfaces of the upper sides of the plurality of test tubes 5 are fixedly connected with limiting plates, the test tubes 5 cannot fall off, after the placing is finished, the rotating cover 4 is screwed on, the valve 9 is unscrewed to enable liquid nitrogen to enter the device, the motor 32 is started, the first bevel gear 33 drives the second bevel gear 34 meshed with the first bevel gear 33 to rotate under the action of the motor 32, the second bevel gear 34 drives the driven mechanism 37 to rotate through the first rotating rod 35, and because the second rotating rod 371 is driven by the first rotating rod 35, the shaft core 374 rotates under the action of the plurality of steel balls 375, and further the two placing discs 372 positioned on the outer surfaces of the shaft core 374 are driven to rotate, finally, the effect of rotating the plurality of test tubes 5 is achieved, and the T cells in each test tube 5 can be approximately the same in intensity of being covered by the liquid nitrogen through the adjusting device.
In order to minimize the influence of the device on the internal environment of the device when the device can access T cells, and to realize that only one tube can be stored or taken in at a time, referring to fig. 1 and 3-5, the pushing mechanism 2 comprises a push rod 21, the left end of the push rod 21 is fixedly connected with a connecting sheet 22, the right end of the connecting sheet 22 is fixedly connected with a spring 24, the left end of the connecting sheet 22 is fixedly connected with a pushing block 23, and the lower end of the pushing block 23 is slidably connected with a chute block 25.
When the T cells need to be taken out, the motor 32 is stopped, the rotating disc 36 is observed through the transparent plate 11 and manually adjusted, the test tube 5 to be taken out is adjusted to the upper part of the pushing mechanism 2, then the rotating cover 4 is unscrewed, the push rod 21 is pushed, the push block 23 slides forwards in the sliding groove block 25, the test tube 5 is jacked up, the hole blocked by the rotating cover 4 is jacked out, and because the right end of the spring 24 is fixedly connected with the left end of the shell 1, when the push rod 21 pushes, the spring 24 deforms, when the pushing force disappears, the push block 23 returns to the initial position under the action of the spring 24, and the rotating cover 4 is screwed up after the test tube 5 is taken out.
In order to ensure the stability of the liquid nitrogen bottle during use, referring to fig. 1 and 6, the fixing hoop 8 is provided with three arc plates 82, the inner surfaces of the three arc plates 82 are fixedly connected with a silica gel pad 83, one ends of the three arc plates 82, which are close to each other in pairs, are fixedly connected with arc telescopic rods 84, the outer surfaces of the arc plates 82 positioned at the leftmost side of the three arc plates 82 are fixedly connected with connectors 81, and the right ends of the connectors 81 are fixedly connected with the left end of the shell 1.
After the liquid nitrogen bottle 7 passes through the fixing hoop 8 and is placed above the placing plate 6, under the action of the three arc-shaped telescopic rods 84, the two arc-shaped plates 82 which are not connected with the shell 1 are inwards extruded until the silica gel pads 83 are tightly attached to the outer surface of the liquid nitrogen bottle 7, and the liquid nitrogen bottle 7 is fixed.
It should be noted that, the specific installation method of the motor 32, the liquid nitrogen bottle 7, the valve 9, the connection method of the circuit and the control method in the present utility model are all conventional designs, and the present utility model is not described in detail.
The working principle of the utility model is as follows: when the device is needed to be used, the rotary cover 4 is unscrewed firstly, a plurality of test tubes 5 are filled into the plurality of placing holes 373 through the opening at the lower part, the rotary cover 4 is screwed on, the motor 32 is started, the valve 9 is opened, and each test tube 5 is regulated to be in full contact with liquid nitrogen, so that T cells are stored.
When the T cells are needed, the motor 32 stops working, the position of the taken-out test tube 5 is adjusted below the rotary cover 4 by using the rotary disc 36 through the transparent plate 11, the rotary cover 4 is unscrewed, the push rod 21 is pushed, the taken-out test tube 5 is pushed out by using the push block 23, the rotary cover 4 is quickly screwed on after the upper part is taken away manually, and the motor 32 is started again.
The foregoing has shown and described the basic principles and main features of the present utility model and the advantages of the present utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made without departing from the spirit and scope of the utility model, which is defined in the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (6)
1. A regulatory T cell preservation device comprising a housing (1), characterized in that: the utility model discloses a liquid nitrogen bottle, including shell (1), fixed plate (6), fixed hoop (8) are fixed to shell (1), fixed plate (6) are placed to shell (1) upper end fixedly connected with slewing mechanism (3), inside swing joint of slewing mechanism (3) has a plurality of test tubes (5), shell (1) upper end threaded connection has rotation lid (4), four cushion (12) of shell (1) lower extreme fixedly connected with, two common fixedly connected with transparent plate (11) of wall about shell (1) inner chamber, shell (1) left end fixedly connected with places board (6), place board (6) upper end swing joint liquid nitrogen bottle (7), liquid nitrogen bottle (7) surface swing joint has fixed hoop (8), liquid nitrogen bottle (7) upper end fixedly connected with valve (9), valve (9) right-hand member fixedly connected with connecting pipe (10), shell (1) right-hand member sliding connection pushing mechanism (2).
2. A regulatory T cell preservation apparatus according to claim 1, characterized in that: the rotating mechanism (3) comprises a fixed plate (31), the lower end of the fixed plate (31) is fixedly connected with the upper end of the shell (1), the upper end of the fixed plate (31) is fixedly connected with a motor (32), the output end of the motor (32) is fixedly connected with a first bevel gear (33) through a coupler, the outer surface of the first bevel gear (33) is in meshed connection with a second bevel gear (34), the lower end of the second bevel gear (34) is fixedly connected with a first rotating rod (35), the outer surface of the first rotating rod (35) is fixedly connected with a rotating disc (36), and the lower end of the first rotating rod (35) is fixedly connected with a driven mechanism (37).
3. A regulatory T cell preservation apparatus according to claim 2, characterized in that: the driven mechanism (37) comprises a rotating rod II (371), two placing discs (372) are fixedly connected to the outer surface of the rotating rod II (371), a plurality of placing holes (373) which vertically penetrate through the placing discs (372) are formed in the upper ends of the placing discs (372), a shaft core (374) is fixedly connected to the lower end of the rotating rod II (371), a plurality of steel balls (375) are movably connected to the outer surface of the shaft core (374), two outer shafts (376) are movably connected to the outer surface of the steel balls (375), a shell (377) is fixedly connected to the outer surface of the outer shaft, and the lower end of the shell (377) is fixedly connected with the bottom wall of an inner cavity of the shell (1).
4. A regulatory T cell preservation apparatus according to claim 1, characterized in that: the pushing mechanism (2) comprises a pushing rod (21), the left end of the pushing rod (21) is fixedly connected with a connecting sheet (22), the right end of the connecting sheet (22) is fixedly connected with a spring (24), the left end of the connecting sheet (22) is fixedly connected with a pushing block (23), and the lower end of the pushing block (23) is slidably connected with a sliding groove block (25).
5. A regulatory T cell preservation apparatus according to claim 1, characterized in that: the fixing hoop (8) is provided with three arc plates (82), three the fixed surface of arc plate (82) is connected with silica gel pad (83), three the equal fixedly connected with arc telescopic link (84) of one end that is close to each other between arc plate (82) two by two, three arc plate (82) are located arc plate (82) surface fixedly connected with joint (81) in leftmost, joint (81) right-hand member and shell (1) left end fixed connection.
6. A regulatory T cell preservation apparatus according to claim 1, characterized in that: and a plurality of limiting sheets are fixedly connected to the outer surfaces of the upper sides of the test tubes (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322686816.1U CN221203871U (en) | 2023-10-08 | 2023-10-08 | Regulatory T cell preservation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322686816.1U CN221203871U (en) | 2023-10-08 | 2023-10-08 | Regulatory T cell preservation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221203871U true CN221203871U (en) | 2024-06-25 |
Family
ID=91540962
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322686816.1U Active CN221203871U (en) | 2023-10-08 | 2023-10-08 | Regulatory T cell preservation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221203871U (en) |
-
2023
- 2023-10-08 CN CN202322686816.1U patent/CN221203871U/en active Active
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