CN221071372U - Silk protein solution production system - Google Patents

Abstract

The utility model discloses a production system of silk fibroin solution, which comprises a constant temperature heating box, wherein the upper end of the constant temperature heating box is provided with a feed port, the discharge port of the constant temperature heating box is connected with the feed port of a pH adjusting tank through a first pipeline, a first pump and a first valve are arranged on the first pipeline, the upper end of the pH adjusting tank is connected with a reagent box through an adjusting pipe, an adjusting valve is arranged on the adjusting pipe, a pH meter is arranged on the pH adjusting tank, the discharge end of the pH adjusting tank is connected with a centrifugal machine through a second pipeline, the second pipeline is provided with a second valve, the first discharge end of the centrifugal machine is connected with a temporary storage tank through a third pipeline, the temporary storage tank is connected with the feed end of an evaporator through a fourth pipeline, the discharge end of the evaporator is connected with a finished product tank through a fifth pipeline. The method is simple to operate, and effectively improves the production efficiency of the silk protein solution with the concentration of 15% -20%.

Description

Silk protein solution production system

Technical Field

The utility model relates to the technical field of silk proteins, in particular to a silk protein solution production system.

Background

Silk proteins are proteins with powerful physical properties and biocompatibility that can be used in a variety of applications. The following are applications of 15-20% silk proteins in different fields:

1. And (3) fiber manufacturing: silk proteins can be used to make high strength and high elastic fibers. The fibers can be applied to the fields of textiles, bandages, sutures and the like.

2. Medical field: silk proteins have good biocompatibility and biodegradability and can be used for preparing absorbable sutures, scaffolds and tissue engineering materials.

3. The food industry: silk protein is a natural food additive, and can improve the texture and stability of food. It can be used for preparing dairy products, frozen foods, pastries, etc.

4. Cosmetic field: the silk proteins can be used for the manufacture of skin care products and cosmetics. It has effects in keeping moisture, resisting oxidation, and protecting skin.

5. Material science: silk proteins have the ability to modulate optical, electrical and thermal properties. Therefore, it can be applied to the fields of optoelectronic devices, sensors, nano materials and the like.

6. Biomedical engineering: the silk protein can be used for manufacturing biomedical engineering applications such as tissue engineering scaffolds, artificial blood vessels, artificial organs and the like.

Note that the specific application of silk proteins in different fields may vary, and that specific use cases may need to be adapted and optimized according to specific needs and studies.

For silk protein concentrations, there is no fixed standard to measure what concentration is high. The concentration ranges applicable may vary from application to application and from need to need.

In general, however, a silk protein concentration of 15-20% can be considered a relatively high concentration. This concentration may provide sufficient silk protein quality to meet the requirements of certain applications. The specific concentration requirements will also depend on the specific application or the objectives of the experiment.

It is important to remember that the properties of silk proteins, solubility, and in particular applications, performance are affected by concentration. Too high or too low a concentration may affect the properties and performance of the material. Thus, in selecting silk protein concentrations, assessment and determination should be made based on specific problems and objectives.

Silk proteins at 15-20% concentration can have some challenges and difficulties in the extraction process, depending primarily on several factors:

1. Solubility: as the concentration of silk proteins increases, their solubility may decrease. Higher concentrations of silk proteins may be more difficult to solubilize and maintain their structural stability. Therefore, proper dissolving agents, pH adjustment and stirring conditions are required to ensure the dissolution and stability of silk proteins.

2. Filtering and purifying: more impurities and insoluble materials may be present in the high concentration silk fibroin solution. This may lead to more difficult filtration and purification processes. Efficient filters and purification methods are required to remove impurities and obtain high purity silk proteins.

3. Protein aggregation: at higher concentrations, silk proteins are prone to aggregation and clotting. This may result in a loss of structure and function of the silk proteins. Thus, measures need to be taken during the extraction to prevent aggregation of the silk proteins, for example using appropriate buffers, temperature control and shear forces.

4. Concentrating and drying: the concentration and drying process of high concentration silk proteins may be more complex. Appropriate techniques and equipment are required to control the rate of concentration, prevent aggregation and maintain stability of the silk proteins.

Thus, the difficulty of extraction at a silk protein concentration of 15-20% is the challenges in handling solubility, filtration and purification, protein aggregation, concentration and drying.

Disclosure of utility model

In view of the above-described drawbacks of the prior art, it is an object of the present utility model to provide a production system for silk protein solutions, which overcomes these difficulties by careful experimental design and tuning of the extraction conditions to obtain silk proteins of the desired concentration.

The technical scheme of the utility model is as follows:

The utility model provides a silk fibroin solution's production system, includes the constant temperature heating cabinet, the upper end of constant temperature heating cabinet sets up the feed port, the feed inlet of pH adjustment tank is connected through first pipeline to the discharge gate of constant temperature heating cabinet, set up first pump and first valve on the first pipeline, the upper end of pH adjustment tank is through adjusting tube connector case, be provided with the governing valve on the adjusting tube, be provided with the pH meter on the pH adjustment tank, the discharge end of pH adjustment tank is through second pipeline connection centrifuge, set up the second valve on the second pipeline, the first discharge end of centrifuge is through third pipeline connection temporary storage tank, the feed end of evaporator is connected through fourth pipeline to the temporary storage tank, set up second pump and third valve on the fourth pipeline, the discharge end of evaporator is through fifth pipeline connection finished product jar.

Preferably, an electric heating assembly and a temperature sensor are arranged on the inner wall of the constant temperature heating box, a signal output end of the temperature sensor is connected with a signal input end of a temperature controller, and a signal output end of the temperature controller is connected with the electric heating assembly.

Preferably, the electric heating component is a thermal resistance wire or an electric heating coil.

Preferably, the lower extreme of constant temperature heating cabinet is provided with the sediment discharge gate, the one end of spiral feeder is connected through first back flow to the sediment discharge gate, the return line is connected to the other end of spiral feeder, be provided with the return valve on the first back flow, the back flow say set up in on the constant temperature heating cabinet.

Preferably, the second discharge end of the centrifuge is connected to the first return pipe through a second return pipe.

Preferably, a sodium hydroxide solution is provided in the constant temperature heating box.

Preferably, the concentration of the sodium hydroxide solution is 2%.

Preferably, hydrochloric acid is provided in the reagent tank.

Preferably, a stirrer is arranged in the pH adjusting tank.

Preferably, the stirrer comprises a stirring motor, a stirring shaft and stirring blades, wherein the stirring motor is arranged at the upper end of the pH adjusting tank, the stirring shaft is arranged inside the pH adjusting tank, the output end of the stirring motor is connected with the stirring shaft, and the stirring blades are arranged on the stirring shaft.

Compared with the prior art, the utility model has the beneficial effects that:

According to the silk protein solution production system, the silk after pupation removal is used as a raw material, the silk is subjected to heat alkali treatment at a constant temperature in a constant temperature heating box in which alkali liquor is stored, the pH value of the treated solution is regulated to a preset pH value range (7-7.5) through a pH regulating tank, and the solution after pH regulation is subjected to negative pressure concentration through an evaporator to obtain the silk protein solution with the concentration of 15% -20%. The system is simple to operate, and improves the production efficiency of the silk protein solution with the concentration of 15% -20%.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to the structures shown in these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a block diagram of a silk fibroin solution production system in accordance with an embodiment of the present utility model.

Fig. 2 is a block diagram of a silk fibroin solution production system provided with a precipitation reflux, in accordance with an embodiment of the present utility model.

FIG. 3 is a flow chart of the operation of a silk fibroin solution production system in accordance with an embodiment of the present utility model.

In the figure, 1 is a constant temperature heating box, 2 is a feed port, 3 is a first pipeline, 4 is a pH adjusting tank, 5 is a first pump, 6 is a first valve, 7 is an adjusting pipe, 8 is a reagent tank, 9 is an adjusting valve, 10 is a pH meter, 11 is a second pipeline, 12 is a centrifugal machine, 13 is a second valve, 14 is a third pipeline, 15 is a temporary storage tank, 16 is a fourth pipeline, 17 is an evaporator, 18 is a second pump, 19 is a third valve, 20 is a fifth pipeline, 21 is a finished product tank, 22 is a bracket, 23 is an electric heating component, 24 is a temperature sensor, 25 is a temperature controller, 26 is a stirrer, 2601 is a stirring motor, 2602 is a stirring shaft, 2603 is a stirring blade, 27 is a precipitation discharge port, 28 is a first reflux pipe, 29 is a second reflux pipe, 30 is a spiral feeder, 31 is a reflux pipeline, and 32 is a reflux valve.

Detailed Description

Other advantages and effects of the present utility model will become apparent to those skilled in the art from the following disclosure, which describes the embodiments of the present utility model with reference to specific examples. The utility model may be practiced or carried out in other embodiments that depart from the specific details, and the details of the present description may be modified or varied from the spirit and scope of the present utility model. It should be noted that the following embodiments and features in the embodiments may be combined with each other without conflict.

In the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front," "rear," "head," "tail," and the like are used as an orientation or positional relationship based on that shown in the drawings, merely to facilitate description of the utility model and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the utility model. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "connected," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

The utility model will now be further described with reference to the accompanying drawings.

The embodiment of the utility model provides a production system of silk fibroin solution, as shown in fig. 1, the production system of silk fibroin solution comprises a constant temperature heating box 1, wherein a feed port 2 (which can be realized as a hopper structure) is arranged at the upper end of the constant temperature heating box 1, a discharge port of the constant temperature heating box 1 is connected with a feed port of a pH regulating tank 4 through a first pipeline 3, a first pump 5 and a first valve 6 are arranged on the first pipeline 3, the upper end of the pH regulating tank 4 is connected with a reagent tank 8 through a regulating pipe 7, a regulating valve 9 is arranged on the regulating pipe 7, a pH meter 10 is arranged on the pH regulating tank 4, a discharge end of the pH regulating tank 4 is connected with a centrifugal machine 12 through a second pipeline 11, a second valve 13 is arranged on the second pipeline 11, a first discharge end of the centrifugal machine 12 is connected with a temporary storage tank 15 through a third pipeline 14, the temporary storage tank 15 is connected with a feed end of an evaporator 17 through a fourth pipeline 16, a second pump 18 and a third valve 19 are arranged on the fourth pipeline 16, and a discharge end of the evaporator 17 is connected with a finished product tank 21 through a fifth pipeline 20.

Please combine fig. 3, fig. 3 is a workflow diagram of the silk protein solution production system, firstly, the silk after pupation is sent into the constant temperature heating box 1 through the feeding port 2, the constant temperature heating box 1 carries out heat alkali treatment to the silk after pupation, the sodium hydroxide solution with concentration of 2% is arranged in the constant temperature heating box 1, the temperature in the constant temperature heating box 1 is controlled to be 100 ℃, the silk is subjected to heat alkali treatment for 2 hours, the supernatant after treatment is pumped to the pH adjusting tank 4 through the first pipeline 3 by opening the first pump 5 and the first valve 6, wherein one end of the first pipeline 3 should be arranged in the constant temperature heating box 1 so as to effectively absorb the supernatant. After the supernatant enters the pH adjusting tank 4, the pH of the solution is adjusted according to the pH value displayed by the pH meter 10, and the acidic solution, preferably hydrochloric acid, is stored in the agent tank 8 due to the heat alkali treatment, the adjusting valve 9 is opened, the hydrochloric acid solution in the agent tank 8 flows into the pH adjusting tank 4 through the adjusting pipe 7, wherein the agent tank 8 can be supported and fixed at the upper end of the pH adjusting tank 4 through the support 22, so that the hydrochloric acid solution in the agent tank 8 flows downwards by gravity, the adjusting valve 9 is opened intermittently, the pH value displayed by the pH meter 10 is read after a proper amount of hydrochloric acid solution is dripped each time, and the adjustment of the pH is completed after the pH value is displayed as 7-7.5. At this time, the second valve 13 is opened to allow the solution with pH of 7-7.5 to enter the centrifuge 12, the centrifuge 12 centrifuging the solution at room temperature and a rotational speed of 4000r/min, most of insoluble precipitate is removed from the centrifuged solution, the centrifuged solution is sent to the temporary storage tank 15 for temporary storage through the third pipeline 14, the temporary storage tank 15 is temporarily stored, the temporary storage tank 15 is pumped to the evaporator 17 for concentration through the fourth pipeline 16 by opening the second pump 18 and the third valve 19, and the silk fibroin solution with concentration of 15% -20% finally obtained by concentration enters the finished product tank 21 for storage through the fifth pipeline 20. Wherein the evaporator 17 is preferably a negative pressure concentrating evaporator.

In some embodiments, a specific structure of the constant temperature heating box is provided, as shown in fig. 1, an electric heating assembly 23 and a temperature sensor 24 are arranged on the inner wall of the constant temperature heating box 1, a signal output end of the temperature sensor 24 is connected with a signal input end of a temperature controller 25, and a signal output end of the temperature controller 25 is connected with the electric heating assembly 23.

The electrothermal component 23 is a heating element capable of generating heat when energized, such as a thermal resistance wire or an electrothermal coil, etc., the electrothermal component 23 is arranged on the inner side wall of the constant temperature heating box 1, the temperature sensor 24 is used for detecting an inner temperature signal of the constant temperature heating box 1 and feeding the temperature signal to the temperature controller 25, and the temperature controller 25 controls the electrothermal component 23 to work when the temperature signal is smaller than a preset temperature threshold value, and controls the electrothermal component 23 to stop working when the temperature signal is larger than or equal to the preset temperature threshold value, so as to control the temperature inside the constant temperature heating box 1 to be always in a constant temperature threshold value range.

In some embodiments, to increase the efficiency of the pH adjustment, an agitator 26 is provided within the pH adjustment tank 4. This agitator includes agitator motor 2601, (mixing) shaft 2602 and stirring vane 2603, agitator motor 2601 set up in the upper end of pH adjustment tank 4, agitator shaft 2602 set up in inside the pH adjustment tank 4, agitator motor 2601's output is connected (mixing) shaft 2602, be provided with on the (mixing) shaft 2602 stirring vane 2603.

When pH is adjusted, the stirring shaft 2602 is driven to rotate by the stirring motor 2601, so that the stirring blade 2603 drives liquid inside the pH adjusting tank 4 to move, so that the acidic reagent is quickly mixed with the solution as much as possible, and alkali in the solution is neutralized, so that the efficiency of pH adjustment is improved.

In some embodiments, considering that there is a different degree of precipitation of undissolved silk proteins in both the thermostated heating tank 1 and the centrifuge 12 after treatment, a reflux structure is designed in order to avoid waste of raw materials. Specifically, referring to fig. 2, a precipitation discharge port 27 is disposed at the lower end of the constant temperature heating box 1, the precipitation discharge port 27 is connected to one end of a screw feeder 30 through a first return pipe 28, the other end of the screw feeder 30 is connected to a return pipe 31, a return valve 32 is disposed on the first return pipe 28, and the return pipe 31 is disposed on the constant temperature heating box 1.

After the constant temperature heating box 1 carries out one round of heat alkali treatment on the pupa-removed silk, the supernatant is pumped to the pH adjusting tank 4 through the first pipeline 3, a certain amount of sediment is remained at the moment, the reflux valve 32 is opened, the sediment enters the screw feeder 30 through the first reflux pipe 28, is transported to the reflux pipeline 31 through the screw feeder 30, finally flows back to the constant temperature heating box 1 through the reflux pipeline 31, and the supernatant and the raw materials of the next round are subjected to heat alkali treatment together or are subjected to secondary heat alkali treatment independently, so that the loss of the raw materials can be avoided.

The centrifuge 12 serves as a centrifuge which likewise separates a precipitate containing undissolved silk proteins and is therefore connected to the first return line 28 at the second discharge end of the centrifuge 12 via a second return line 29. The precipitate produced by the centrifuge 12 during centrifugation is fed into the first return pipe 28 through the second return pipe 29, is transported to the return pipe 31 through the screw feeder 30, and is finally returned to the constant temperature heating tank 1 through the return pipe 31.

It should be noted that a screw feeder may also be provided on the second return pipe 29 to effect the transport of the sediment produced by the centrifuge 12.

The above embodiments are only for illustrating the technical solution of the present utility model, and not for limiting the same; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the utility model, and are intended to be included within the scope of the appended claims and description.

Claims (9)

1. The utility model provides a production system of silk fibroin solution, its characterized in that, includes the constant temperature heating cabinet, the upper end of constant temperature heating cabinet sets up the feed port, the feed inlet of pH adjustment tank is connected through first pipeline to the discharge gate of constant temperature heating cabinet, set up first pump and first valve on the first pipeline, the upper end of pH adjustment tank is through adjusting tube connector case, be provided with the governing valve on the adjusting tube, be provided with the pH meter on the pH adjustment tank, the discharge end of pH adjustment tank is through second pipeline connection centrifuge, set up the second valve on the second pipeline, the first discharge end of centrifuge is through the third pipeline connection temporary storage tank, the feed end of evaporimeter is connected through the fourth pipeline to the temporary storage tank, set up second pump and third valve on the fourth pipeline, the discharge end of evaporimeter is through fifth pipeline connection finished product jar.

2. The silk fibroin solution production system according to claim 1, wherein an electric heating assembly and a temperature sensor are arranged on the inner wall of the constant temperature heating box, a signal output end of the temperature sensor is connected with a signal input end of a temperature controller, and a signal output end of the temperature controller is connected with the electric heating assembly.

3. The system for producing silk fibroin solution according to claim 2, wherein the electric heating component is a thermal resistance wire or an electric heating coil.

4. The production system of silk fibroin solution according to claim 1, wherein a precipitation discharge port is arranged at the lower end of the constant temperature heating box, the precipitation discharge port is connected with one end of the screw feeder through a first return pipe, the other end of the screw feeder is connected with a return pipe, a return valve is arranged on the first return pipe, and the return pipe is arranged on the constant temperature heating box.

5. The silk fibroin solution production system of claim 4, wherein the second discharge end of the centrifuge is connected to the first return tube through a second return tube.

6. The system for producing silk fibroin solution according to claim 1, wherein sodium hydroxide solution is provided in the constant temperature heating box.

7. The silk fibroin solution production system of claim 1, wherein hydrochloric acid is disposed in the agent tank.

8. The silk fibroin solution production system of claim 1, wherein a stirrer is provided in the pH adjustment tank.

9. The silk fibroin solution production system of claim 8, wherein the stirrer comprises a stirring motor, a stirring shaft and stirring blades, the stirring motor is arranged at the upper end of the pH adjusting tank, the stirring shaft is arranged inside the pH adjusting tank, the output end of the stirring motor is connected with the stirring shaft, and the stirring blades are arranged on the stirring shaft.