CN220912984U - Optical imaging device and gene sequencing equipment - Google Patents

Abstract

The utility model discloses an optical imaging device and a gene sequencing device. The optical imaging device comprises a bearing table, an objective lens arranged on the bearing table, a light splitting mechanism arranged on the bearing table, four cameras and a first supporting seat, wherein the light splitting mechanism is used for splitting light rays from the objective lens into different first light beams, second light beams, third light beams and fourth light beams, the four cameras are respectively positioned on light paths of the four light beams and used for receiving corresponding light beams and forming images, the first camera, the second camera, the third camera and the first supporting seat are arranged on the bearing table, the fourth camera is arranged on the first supporting seat, the distance between any one of the first camera, the second camera and the third camera and the bearing table is a first distance, the distance between the fourth camera and the bearing table is a second distance, and the second distance is larger than the first distance. The optical imaging device of the embodiment of the utility model is horizontally arranged, and adopts a double-layer structure, so that the space utilization rate in the horizontal direction is high, the structure is compact, and the sequencing efficiency is high.

Description

Optical imaging device and gene sequencing equipment

Technical Field

The utility model relates to the technical field of gene sequencing, in particular to an optical imaging device and gene sequencing equipment.

Background

Gene sequencing technology means a technical means for obtaining DNA or RNA base sequences by detection. The current dominant sequencing technology is a high-throughput sequencing technology, in which the general process of gene sequencing includes fixing a nucleic acid sample to be tested on a biochip in a hybridization manner, amplifying by PCR to form nucleic acid molecular clusters on the nucleic acid sample to be tested, adding a base with a fluorescent group, polymerase, primer and the like, combining the base with the fluorescent group with the base on the nucleic acid sample to be tested by a base complementary pairing principle, exciting the fluorescent group by an optical path imaging system to generate fluorescence, collecting the fluorescence to form an image, and performing base identification by the image, thereby realizing the base sequence determination of the nucleic acid sample to be tested.

At present, two layouts of an optical imaging system structure of the gene sequencer are provided, one is that the whole optical system is vertically placed, the other is that the whole optical system is horizontally placed, and generally, the multi-channel imaging systems are all placed on the same datum plane, so that the optical imaging systems need more space on the same horizontal plane.

Disclosure of utility model

The utility model provides an optical imaging device and a gene sequencing device.

The optical imaging device comprises a bearing table, an objective lens arranged on the bearing table, a light splitting mechanism, a first camera, a second camera, a third camera, a fourth camera and a first supporting seat, wherein the light splitting mechanism is used for splitting light rays from the objective lens into different first light beams, second light beams, third light beams and fourth light beams, the first camera is positioned on an optical path of the first light beams and used for receiving the first light beams and forming images, the second camera is positioned on an optical path of the second light beams and used for receiving the second light beams and forming images, the third camera is positioned on an optical path of the third light beams and used for receiving the third light beams and forming images, the fourth camera is positioned on an optical path of the fourth light beams and used for receiving the fourth light beams and forming images, the first camera, the second camera and the third camera are arranged on the bearing table, the first supporting seat is arranged on the bearing table, the distance between any one of the first camera, the second camera and the third camera is a first distance from the bearing table, and the fourth camera is a second distance from the bearing table, and the second distance between the fourth camera is a second distance from the bearing table is larger than the first distance.

According to the optical imaging device, the first camera, the second camera and the third camera are arranged in the horizontal direction of the bearing table, and the fourth camera is arranged in the vertical direction of the bearing table, so that the space utilization rate of the optical imaging device in the horizontal direction is high, the volume of the optical imaging device is reduced, the structure is compact, fluorescent signals corresponding to various alkali groups can be collected at the same time, and the information collection efficiency in the sequencing process is high.

In some embodiments, the table top of the stage is perpendicular to the optical axis of the objective lens.

Therefore, when the sample to be measured is positioned below the bearing table and is arranged in parallel with the table top of the bearing table, the optical axis of the objective lens is perpendicular to the sample to be measured, so that the objective lens can receive light from the sample to be measured, and fluorescence information of the sample to be measured can be collected.

In some embodiments, the first camera and the second camera are respectively located at two opposite sides of the first support base.

Thus, by reducing the distance between the first camera and the second camera and the first supporting seat, the volume of the optical imaging device can be reduced, and the optical imaging device is compact.

In some embodiments, the optical imaging device includes a first mount secured to the carrier, the first camera being secured to the first mount.

Therefore, the first camera can be fixed on the bearing table through the first bracket, shake of the first camera is reduced, and imaging stability of the optical imaging device is improved.

In some embodiments, the optical imaging device includes a second mount secured to the carrier, and the second camera is secured to the second mount.

Therefore, the second camera can be fixed on the bearing table through the second support, shake of the second camera is reduced, and imaging stability of the optical imaging device is improved.

In some embodiments, the optical imaging device includes a third mount secured to the carrier, and the third camera is secured to the third mount.

Therefore, the third camera can be fixed on the bearing table through the third support, shake of the third camera is reduced, and imaging stability of the optical imaging device is improved.

In some embodiments, the optical imaging apparatus includes a fourth bracket fixed to the first support, and the fourth camera is fixed to the fourth bracket.

Therefore, the fourth camera can be fixed on the first supporting seat through the fourth bracket, shake of the fourth camera is reduced, and imaging stability of the optical imaging device is improved.

In some embodiments, the light splitting mechanism includes a first light splitting mechanism, a second light splitting mechanism, a third light splitting mechanism and a fourth light splitting mechanism, the first light splitting mechanism splits light from the objective lens to form a first mixed beam and a fourth light beam, the first light splitting mechanism is further configured to split a part of the first mixed beam to form a first beam and to converge the first beam to the first camera, another part of the first mixed beam is transmitted as a second mixed beam to the second light splitting mechanism, the second light splitting mechanism is configured to receive the second mixed beam, split the second mixed beam to form a second beam and a third beam and to converge the second beam to the second camera, the third light splitting mechanism is configured to converge the third beam to the third camera, and the fourth light splitting mechanism is configured to receive the fourth light beam formed by the first light splitting mechanism and to converge the fourth beam to the fourth camera.

The light from the objective lens is split by the light splitting mechanism to form a plurality of light beams, and the light beams are converged to the corresponding cameras for imaging, so that different light rays emitted by the sample to be detected are detected.

In some embodiments, the optical imaging device includes a second support mounted on the stage, the second support being juxtaposed with the first support, the first light splitting mechanism including a first dichroic mirror disposed on the optical axis of the objective lens and mounted on the second support and disposed obliquely with respect to the optical axis of the objective lens, the first dichroic mirror for reflecting light from the objective lens to form a first mixed light beam and transmitting light from the objective lens to form a fourth light beam, and a second dichroic mirror mounted on the stage spaced from the first dichroic mirror for reflecting a portion of the first mixed light beam to form a first light beam, the first light beam being directed to the first camera; and transmits another portion of the first light beam to form a second mixed light beam.

In this way, the first dichroic mirror can be fixed by being mounted on the second supporting seat, so that the first dichroic mirror can stably split light from the objective lens to form a first mixed beam and a fourth beam, and meanwhile, the second dichroic mirror can be fixed by being mounted on the bearing table, so that the second dichroic mirror can stably split the first mixed beam to form the first beam and the second mixed beam, the loss of the beam in the first splitting mechanism is reduced, the utilization rate of the beam is increased, and the imaging effect of the optical imaging device is improved. In addition, the first dichroic mirror and the second dichroic mirror are mounted on the bearing table, so that light can be turned and spread in the horizontal and vertical directions, and the first camera and the fourth camera can be arranged at different positions of the bearing table, thereby improving the space utilization rate of the optical imaging device and enabling the structure of the optical imaging device to be more compact.

In some embodiments, the first light splitting mechanism further comprises a first mirror mounted on the carrier, the first mirror being located on one side of the first support and spaced apart from the second dichroic mirror, the first mirror for reflecting a portion of the first light beam from the second dichroic mirror toward the first camera.

Therefore, the first reflecting mirror can be fixed on the bearing table by installing the first reflecting mirror, so that the first reflecting mirror can stably reflect the first light beam to the first camera, and the imaging stability of the first camera is improved. Meanwhile, the first reflecting mirror is arranged on the bearing table, so that the first light beam can be turned and spread on the table top, the space utilization rate of the optical imaging device in the horizontal direction is improved, and the structure of the optical imaging device is more compact.

In some embodiments, the second dichroic mirror is at least partially disposed within a first support formed with a first light channel between the second dichroic mirror and the first mirror.

Thus, the first light beam formed by reflecting the first mixed light beam by the second dichroic mirror can be transmitted to the first reflecting mirror through the first light channel, and then reflected to the first camera by the first reflecting mirror for imaging.

In some embodiments, the second dichroic mechanism includes a third dichroic mirror mounted on the stage, the third dichroic mirror being disposed at a distance from the second dichroic mirror, the third dichroic mirror being disposed on a side of the second dichroic mirror facing away from the first dichroic mirror, the third dichroic mirror being configured to reflect a portion of the second mixed light beam to form a second light beam, direct the second light beam to the second camera, and transmit another portion of the second mixed light beam to form a third light beam.

Therefore, the third dichroic mirror can be fixed by being arranged on the bearing table, so that the third dichroic mirror can stably split the second mixed light beam to form the second light beam and the third light beam, meanwhile, the third dichroic mirror is arranged on the bearing table, the light splitting of the first mixed light beam can be realized, the split light beam can be transmitted on the table top, the second camera and the third camera can be further arranged at different positions of the bearing table, the space utilization rate of the optical imaging device in the horizontal direction is improved, and the structure of the optical imaging device is more compact.

In some embodiments, the third dichroic mirror is disposed within a first support formed with a second light channel between the third dichroic mirror and the second camera.

In this way, a second light beam formed by the third dichroic mirror reflecting the second mixed light beam may propagate through the second light channel to the second camera for imaging.

In some embodiments, the third light splitting mechanism includes a second mirror mounted on the carrier, the second mirror being spaced apart from the third dichroic mirror, the second mirror being disposed on a side of the third dichroic mirror facing away from the second dichroic mirror, the second mirror being configured to reflect the third light beam toward the third camera.

Therefore, the second reflecting mirror can be fixed by being arranged on the bearing table, so that the second reflecting mirror can stably reflect the third light beam to the third camera, and the imaging stability of the third camera is improved. Meanwhile, the second reflecting mirror is arranged on the bearing table, so that the steering of the third light beam can be realized, the third light beam can be transmitted on the table top, the space utilization rate of the optical imaging device in the horizontal direction is improved, and the structure of the optical imaging device is more compact.

In some embodiments, the second mirror is disposed within a first mount that forms a third optical path between the second mirror and a third camera.

Therefore, the third light beam can be transmitted to the third camera for imaging through the third light channel after being reflected by the second reflecting mirror.

In some embodiments, the fourth light splitting mechanism includes a third mirror mounted on the second support, the third mirror being disposed on and inclined with respect to the optical axis of the objective lens, the third mirror being located on a side of the first dichroic mirror away from the objective lens, the third mirror being for reflecting the fourth light beam towards the fourth camera.

Therefore, the third reflector can be fixed on the second supporting seat by installing the third reflector, so that the third reflector can stably reflect the fourth light beam to the fourth camera, and the imaging stability of the fourth camera is improved. Meanwhile, the third reflecting mirror is arranged on the second supporting seat, so that the propagation of the fourth light beam in the vertical direction can be changed into the propagation in the horizontal direction, the fourth camera can be distributed in the vertical direction relative to the bearing table, the space required by the optical imaging device in the horizontal direction is reduced, the space utilization rate of the optical imaging device in the horizontal direction is improved, and the structure of the optical imaging device is more compact.

In some embodiments, the optical imaging apparatus includes a first barrel, a second barrel, a third barrel, and a fourth barrel, each of the first barrel, the second barrel, and the third barrel being mounted on the stage, the first barrel being disposed on an object side of the first camera and configured to converge the first light beam onto the first camera, the second barrel being disposed on an object side of the second camera and configured to converge the second light beam onto the second camera, the third barrel being disposed on an object side of the third camera and configured to converge the third light beam onto the third camera, the fourth barrel being mounted on the first mount, the fourth barrel being disposed on an object side of the fourth camera and configured to converge the fourth light beam onto the fourth camera.

Thus, the first barrel lens can be matched with the objective lens to converge the first light beam to the first camera positioned on the bearing table for imaging, the second barrel lens can be matched with the objective lens to converge the second light beam to the second camera positioned on the bearing table for imaging, the third barrel lens can be matched with the objective lens to converge the third light beam to the third camera positioned on the bearing table for imaging, and the fourth barrel lens can be matched with the objective lens to converge the fourth light beam to the fourth camera positioned on the first supporting seat.

In some embodiments, the optical imaging device includes a second support base and a light source assembly, the second support base is mounted on the carrying platform, the light source assembly is mounted on the second support base and disposed on one side of an optical axis of the objective lens, and the light source assembly is used for emitting an excitation light beam to a sample to be tested through the objective lens.

Therefore, the light source assembly can be fixed on one side of the optical axis of the objective lens through the second supporting seat, so that the light source assembly can stably emit the excitation light beam, and meanwhile, the distance between the light source assembly and the objective lens is reduced, and the optical imaging device is compact in structure.

In some embodiments, the optical imaging device further comprises a fifth bracket and an automatic focusing device mounted on the fifth bracket, the fifth bracket is mounted on the bearing table, the automatic focusing device comprises a second light source and a focusing sensor, and the second light source is used for projecting a light beam emitted by the second light source onto the sample to be measured through the objective lens; the focusing sensor is used for receiving the light beam reflected from the sample to be detected and collimated by the objective lens, and converting the light signal into an electric signal so that the objective lens moves according to the electric signal, and the plane of the sample to be detected is located at the focal plane of the objective lens.

The automatic focusing device is mainly used for marking the object distance after the objective lens finishes focusing, monitoring the change quantity of the object distance in real time in the gene sequencing process, correcting the change quantity, and fixing the automatic focusing device through a fifth bracket so as to ensure that the objective lens is always focused clearly, ensure the accuracy of the image acquired by the detection camera and finally obtain a clear image within the focal depth range.

The genetic sequencing apparatus of the embodiments of the present utility model includes an optical imaging device.

Therefore, the optical imaging device is horizontally distributed and is of a double-layer structure, so that the space utilization rate of the optical imaging device in the horizontal direction is higher, the structure of the optical imaging device is more compact, and the structure of the gene sequencing equipment is more compact.

Additional aspects and advantages of the utility model will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the utility model.

Drawings

The foregoing and/or additional aspects and advantages of the present utility model will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings, in which:

Fig. 1 is a schematic structural view of an optical imaging apparatus according to an embodiment of the present utility model;

FIG. 2 is a schematic view of a stage and objective lens connection according to an embodiment of the present utility model;

FIG. 3 is a side view of an optical imaging apparatus according to an embodiment of the present utility model;

FIG. 4 is a top view of an optical imaging apparatus according to an embodiment of the present utility model;

FIG. 5 is a schematic view of the structure of an optical imaging apparatus according to an embodiment of the present utility model;

FIG. 6 is a schematic diagram of an optical imaging apparatus according to an embodiment of the present utility model;

Fig. 7 is a schematic structural view of an optical imaging apparatus according to an embodiment of the present utility model;

Fig. 8 is a schematic structural view of a second support base according to an embodiment of the present utility model;

fig. 9 is a schematic structural view of an optical imaging apparatus according to an embodiment of the present utility model;

fig. 10 is a schematic structural view of a first support base according to an embodiment of the present utility model;

fig. 11 is a schematic structural view of a first support base according to an embodiment of the present utility model;

Fig. 12 is a schematic structural view of a second support base according to an embodiment of the present utility model;

Reference numerals illustrate: 100. an optical imaging device; 10. an objective lens; 15. an optical axis; 20. a light splitting mechanism; 21. a first camera; 22. a second camera; 23. a third camera; 24. a fourth camera; 31. a first barrel mirror; 32. a second barrel mirror; 33. a third barrel mirror; 34. a fourth barrel mirror; 41. a first optical filter; 42. a second optical filter; 43. a third filter; 44. a fourth optical filter; 50. a light source assembly; 51. a first light source; 52. a first lens; 53. a bandpass filter; 60. an automatic focusing device; 61. a second lens; 62. a second light source; 63. a focus sensor; 64. a fourth dichroic mirror; 70. a carrying platform; 701. a table top; 702. a first mounting member; 703. a second mounting member; 704. a third mount; 705. a fourth mount; 706. a mounting hole; 71. a first support base; 711. a first optical channel; 712. a first through hole; 713. a second optical channel; 714. a second through hole; 715. a third optical channel; 716. a third through hole; 72. a first bracket; 73. a second bracket; 74. a third bracket; 75. a fourth bracket; 76. a second support base; 77. a fifth bracket; 211. a first light beam; 212. a second light beam; 213. a third light beam; 214. a fourth light beam; 221. a first spectroscopic mechanism; 222. a second spectroscopic mechanism; 223. a third light splitting mechanism; 224. a fourth spectroscopic mechanism; 225. a first dichroic mirror; 226. a second dichroic mirror; 227. a first mirror; 228. a third dichroic mirror; 229. a second mirror; 230. a third mirror; 231. a first mixed beam; 232. and a second mixed beam.

Detailed Description

Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only for explaining the present utility model and are not to be construed as limiting the present utility model.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more of the described features. In the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically connected, electrically connected or can be communicated with each other; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present utility model, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.

The following disclosure provides many different embodiments, or examples, for implementing different features of the utility model. In order to simplify the present disclosure, components and arrangements of specific examples are described below. They are, of course, merely examples and are not intended to limit the utility model. Furthermore, the present utility model may repeat reference numerals and/or letters in the various examples, which are for the purpose of brevity and clarity, and which do not themselves indicate the relationship between the various embodiments and/or arrangements discussed. In addition, the present utility model provides examples of various specific processes and materials, but one of ordinary skill in the art will recognize the application of other processes and/or the use of other materials.

Referring to fig. 1 and 2, an optical imaging apparatus 100 according to an embodiment of the present utility model includes a stage 70, an objective lens 10 mounted on the stage 70, a spectroscopic mechanism 20 mounted on the stage 70, a first camera 21, a second camera 22, a third camera 23, and a fourth camera 24, and a first support 71, wherein the spectroscopic mechanism 20 is configured to divide light from the objective lens 10 into different first light beams 211, second light beams 212, third light beams 213, and fourth light beams 214, the first camera 21 is located on an optical path of the first light beams 211 and is configured to receive the first light beams 211 and form images, the second camera 22 is located on an optical path of the second light beams 212 and is configured to receive the second light beams 212 and form images, the third camera 23 is located on an optical path of the third light beams 213 and is configured to receive the fourth light beams 214, the first camera 21, the second camera 22, and the third camera 23 are mounted on the stage 70, the first support 71 is mounted on the stage 70 and is located on any one of the first and second cameras 24, and the second camera 24 are located at a large distance from the stage 70.

In the optical imaging device 100 of the embodiment of the application, the first camera 21, the second camera 22 and the third camera 23 are arranged in the horizontal direction of the bearing table 70, and the fourth camera 24 is arranged in the vertical direction of the bearing table 70, so that the space utilization rate of the optical imaging device 100 in the horizontal direction is high, the volume of the optical imaging device 100 is reduced, the structure is compact, fluorescent signals corresponding to various base groups can be acquired at the same time, and the information acquisition efficiency in the sequencing process is high.

Specifically, in some embodiments, the carrying platform 70 is in a square plate structure, four corners of the carrying platform 70 may adopt a rounded corner design, so as to avoid injury to a human body caused by the rounded corners, a sample platform is disposed below the carrying platform 70, and the sample platform is used for placing a sample to be tested, which may be a nucleic acid sample, for example, a DNA or RNA sample. The bearing table 70 is provided with a mounting hole 706 opposite to the sample platform, the mounting hole 706 penetrates through the upper surface and the lower surface of the bearing table 70, the objective lens 10 penetrates through the mounting hole 706 to be mounted on the bearing table 70 so as to collect fluorescence information of a sample to be measured placed on the sample platform, and the objective lens 10 can be fixed in the mounting hole 706 through a connecting piece. The objective lens 10 is fixed on the bearing table 70 through an objective lens mounting piece, the light splitting mechanism 20, the first camera 21, the second camera 22 and the third camera 23 can be fixed on the bearing table 70 through fasteners such as bolts, the objective lens 10 is used for receiving light rays from a sample to be detected and transmitting the light rays to the light splitting mechanism 20, the light splitting mechanism 20 is used for receiving the light rays from the objective lens 10 and carrying out light splitting treatment on the light rays to form different light beams, the different light beams respectively correspond to the different cameras, and the cameras are used for converting the different light beams into two-dimensional images and serve as raw data of subsequent data processing and analysis links of a gene sequencing technology.

The first supporting seat 71 may be composed of two opposite side plates and a flat plate, the side plates and the flat plate are in a rectangular plate-shaped structure, the flat plate is located at the top of the two side plates, the two side plates are arranged along the direction of the fourth light beam 214, the bottom surfaces of the side plates extend outwards, so that the two side plates can be fixed on the surface of the carrying platform 70 through fasteners such as bolts, and the flat plate is fixed on the surface of the two side plates through fasteners such as bolts, so that the fourth camera 24 can be arranged on the flat plate.

Referring to fig. 3, in some embodiments, a table 701 of the stage 70 is perpendicular to the optical axis 15 of the objective lens 10.

Thus, when the sample to be measured is located below the carrying table 70 and is disposed parallel to the table surface 701 of the carrying table 70, the optical axis 15 of the objective lens 10 is perpendicular to the sample to be measured, so that the objective lens 10 receives the light from the sample to be measured, thereby collecting the fluorescence information of the sample to be measured.

Specifically, the table 701 of the stage 70 may be an upper surface of the stage 70, and the objective lens 10 is fixed to the stage 70 perpendicularly to the table 701 of the stage 70.

Referring to fig. 4, in some embodiments, the first camera 21 and the second camera 22 are respectively located on two opposite sides of the first support 71.

In this way, by reducing the distance between the first camera 21 and the second camera 22 and the first support 71, the volume of the optical imaging apparatus 100 can be reduced, making the optical imaging apparatus 100 compact.

Specifically, the first camera 21 and the second camera 22 are respectively located at two opposite sides of the two side plates of the first support 71.

Further, the second camera 22 and the third camera 23 are located on the same side of the first support 71, that is, the third camera 23 and the first camera 21 are also located on two opposite sides of the first support 71, so as to further improve the compactness of the optical imaging device 100.

Referring to fig. 1, in some embodiments, the optical imaging apparatus 100 includes a first bracket 72 fixed on the carrying platform 70, and the first camera 21 is fixed on the first bracket 72.

In this way, the first camera 21 can be fixed on the carrying platform 70 by the first bracket 72, so as to reduce the shake of the first camera 21 and improve the imaging stability of the optical imaging device 100.

Specifically, the first bracket 72 may be composed of two flat plates, two side plates and a back plate, the side plates, the flat plates and the back plate are in a rectangular plate-shaped structure, the first bracket 72 is disposed towards the opening of the first camera 21, the two flat plates form the upper surface and the lower surface of the first bracket 72, the two side plates are respectively located at two sides of the first camera 21, the back plate is provided with a circular hole, the bottom surface of the first bracket 72 is fixed on the carrying table 70 through fasteners such as bolts, the first camera 21 is fixed on the first bracket 72 through fasteners such as bolts, and the first light beam 211 is converged to the first camera 21 through the circular hole.

Referring again to fig. 1, in some embodiments, the optical imaging apparatus 100 includes a second bracket 73 fixed to the stage 70, and the second camera 22 is fixed to the second bracket 73.

In this way, the second camera 22 can be fixed on the carrying platform 70 by the second bracket 73, so that the shake of the second camera 22 is reduced, and the imaging stability of the optical imaging device 100 is improved.

Specifically, the second bracket 73 may be composed of two flat plates, two side plates and a back plate, where the side plates, the flat plates and the back plate are in a rectangular plate structure, the second bracket 73 is disposed towards the opening of the second camera 22, the two flat plates form the upper and lower surfaces of the second bracket 73, the two side plates are respectively located at two sides of the second camera 22, the back plate is provided with a circular hole, the bottom surface of the second bracket 73 is fixed on the carrying table 70 by a fastener such as a bolt, the second camera 22 is fixed on the second bracket 73 by a fastener such as a bolt, and the second light beam 212 is converged to the second camera 22 through the circular hole.

Referring to fig. 1, in some embodiments, the optical imaging apparatus 100 includes a third bracket 74 fixed on the stage 70, and the third camera 23 is fixed on the third bracket 74.

In this way, the third camera 23 can be fixed on the carrying platform 70 by the third bracket 74, so as to reduce the shake of the third camera 23 and improve the imaging stability of the optical imaging device 100.

Specifically, the third bracket 74 may be composed of two flat plates, two side plates and a back plate, where the side plates, the flat plates and the back plate are in a rectangular plate structure, the third bracket 74 is set towards the opening of the third camera 23, the two flat plates form the upper and lower surfaces of the third bracket 74, the two side plates are respectively located at two sides of the third camera 23, the back plate is provided with a circular hole, the bottom surface of the third bracket 74 is fixed on the bearing table 70 by fasteners such as bolts, the third camera 23 is fixed on the third bracket 74 by fasteners such as bolts, and the third light beam 213 is converged to the third camera 23 through the circular hole.

Referring to fig. 1, in some embodiments, the optical imaging apparatus 100 includes a fourth bracket 75 fixed on the first support 71, and the fourth camera 24 is fixed on the fourth bracket 75.

In this way, the fourth camera 24 can be fixed on the first support base 71 by the fourth bracket 75, so as to reduce the shake of the fourth camera 24 and improve the imaging stability of the optical imaging device 100.

Specifically, the fourth bracket 75 may be composed of two flat plates, two side plates and a back plate, where the side plates, the flat plates and the back plate are in a rectangular plate structure, the fourth bracket 75 is disposed towards the opening of the fourth camera 24, the two flat plates form the upper and lower surfaces of the fourth bracket 75, the two side plates are respectively located at two sides of the fourth camera 24, the back plate is provided with a circular hole, the bottom surface of the fourth bracket 75 is fixed on the first supporting seat 71 by a fastener such as a bolt, the fourth camera 24 is fixed on the fourth bracket 75 by a fastener such as a bolt, and the fourth light beam 214 is converged to the fourth camera 24 through the circular hole.

Referring to fig. 5-7, in some embodiments, the light splitting mechanism 20 includes a first light splitting mechanism 221, a second light splitting mechanism 222, a third light splitting mechanism 223, and a fourth light splitting mechanism 224, where the first light splitting mechanism 221 splits the light from the objective lens 10 to form a first mixed light beam 231 and a fourth light beam 214, the first light splitting mechanism 221 is further configured to split a portion of the first mixed light beam 231 to form a first light beam 211, and to converge the first light beam 211 to the first camera 21, another portion of the first mixed light beam 231 is transmitted as a second mixed light beam 232 to the second light splitting mechanism 222, the second light splitting mechanism 222 is configured to receive the second mixed light beam 232, split the second mixed light beam 232 to form a second light beam 212 and a third light beam 213, and to converge the second light beam 212 to the second camera 22, and the third light beam 213 is transmitted to the third light splitting mechanism 223, the third light splitting mechanism 223 is configured to converge the third light beam 213 to the third camera 23, and the fourth light splitting mechanism 224 receives the fourth light beam 214 formed by the first light splitting mechanism 221 and to converge the fourth light beam 214 to the fourth camera 24.

The light from the objective lens 10 is split by the beam splitting mechanism 20 to form a plurality of light beams, and the light beams are converged to the corresponding cameras for imaging, so that different light rays emitted by the sample to be detected are detected.

Specifically, the beam splitting structure can split the light from the objective lens 10 into two beams, three beams, four beams, and the like, and by arranging the beam splitting mechanism 20, the direction of the light beam can be changed, so that the camera can be located at different positions, the space utilization rate of the optical imaging device 100 is improved, and the structure of the optical imaging device 100 is compact.

Referring to fig. 1 and 5-9, in some embodiments, the optical imaging apparatus 100 includes a second support 76 mounted on the stage 70, the second support 76 is disposed in parallel with the first support 71, the first beam splitting mechanism 221 includes a first dichroic mirror 225 and a second dichroic mirror 226, the first dichroic mirror 225 is disposed on the optical axis 15 of the objective lens 10 and mounted on the second support 76, and is disposed obliquely with respect to the optical axis 15 of the objective lens 10, the first dichroic mirror 225 is configured to reflect light from the objective lens 10 to form a first mixed beam 231 and transmit light from the objective lens 10 to form a fourth beam 214, the second dichroic mirror 226 is mounted on the stage 70 and spaced apart from the first dichroic mirror 225, and the second dichroic mirror 226 is configured to reflect a portion of the first mixed beam 231 to form a first beam 211, direct the first beam 211 to the first camera 21, and transmit another portion of the first beam 211 to form a second mixed beam 232.

As such, by mounting the first dichroic mirror 225 on the second support 76, the first dichroic mirror 225 can be fixed so that the first dichroic mirror 225 can stably split light from the objective lens 10 to form the first mixed light beam 231 and the fourth light beam 214, while by mounting the second dichroic mirror 226 on the stage 70, the second dichroic mirror 226 can be fixed so that the second dichroic mirror 226 can stably split the first mixed light beam 231 to form the first light beam 211 and the second mixed light beam 232, reducing the loss of light beams in the first light splitting mechanism 221, increasing the utilization ratio of light beams, thereby improving the imaging effect of the optical imaging apparatus 100. In addition, the mounting of the first dichroic mirror 225 and the second dichroic mirror 226 on the stage 70 can realize the steering of light and the propagation in the horizontal and vertical directions, so that the first camera 21 and the fourth camera 24 can be disposed at different positions of the stage 70, thereby improving the space utilization of the optical imaging apparatus 100, and making the structure of the optical imaging apparatus 100 more compact.

Specifically, the second support 76 may be a rectangular parallelepiped housing, the second support 76 extends upward from the table surface 701 of the carrying table 70, the bottom surface of the second support 76 is fixed to the carrying table 70 by a fastener such as a bolt, the first dichroic mirror 225 is disposed in the housing of the second support 76 and is fixed to a side wall of the housing by a fastener such as a bolt, and the second dichroic mirror 226 may be fixed to the carrying table 70 by a fastener such as a bolt.

In some embodiments, and referring to fig. 5, first dichroic mirror 225 is disposed at a 45 ° angle with respect to optical axis 15 of objective lens 10, and first dichroic mirror 225 may split light from objective lens 10 to form a first mixed light beam 231 and a fourth light beam 214, first mixed light beam 231 being perpendicular to light from objective lens 10, and fourth light beam 214 being in the same direction as light from objective lens 10. First dichroic mirror 225 and second dichroic mirror 226 are disposed vertically, and second dichroic mirror 226 splits first mixed light beam 231 into a second mixed light beam 232 and a first light beam 211, second mixed light beam 232 being in the same direction as first mixed light beam 231, first light beam 211 being perpendicular to first mixed light beam 231.

Referring to fig. 1 and 5, in some embodiments, the first light splitting mechanism 221 further includes a first mirror 227 mounted on the carrier 70, where the first mirror 227 is located on one side of the first support 71 and spaced apart from the second dichroic mirror 226, and the first mirror 227 is configured to reflect a portion of the first light beam 211 from the second dichroic mirror 226 toward the first camera 21.

In this way, the first mirror 227 may be fixed by mounting the first mirror 227 on the carrier 70, so that the first mirror 227 may stably reflect the first light beam 211 to the first camera 21, thereby improving the imaging stability of the first camera 21. Meanwhile, the first reflecting mirror 227 is mounted on the bearing table 70, so that the first light beam 211 can be turned and spread on the table surface 701, the space utilization rate of the optical imaging device 100 in the horizontal direction is improved, and the structure of the optical imaging device 100 is more compact.

Specifically, the first mirror 227 may be fixed to the stage 70 by a fastener such as a bolt, and in some embodiments, the first mirror 227 may be disposed in parallel with the second dichroic mirror 226.

Referring to fig. 9 and 10, in some embodiments, the second dichroic mirror 226 is at least partially disposed within the first support 71, and the first support 71 is formed with a first light channel 711 between the second dichroic mirror 226 and the first mirror 227.

In this way, the first light beam 211 formed by the second dichroic mirror 226 reflecting the first mixed light beam 231 may propagate to the first mirror 227 through the first light channel 711, and then be reflected by the first mirror 227 to the first camera 21 for imaging.

Specifically, the first support 71 has a first through hole 712 formed near a side plate of the first mirror 227, and the first light beam 211 may propagate from the second dichroic mirror 226 to the first mirror 227 through the first through hole 712.

Referring to fig. 5 and 9, in some embodiments, the second dichroic mechanism 222 includes a third dichroic mirror 228 mounted on the stage 70, the third dichroic mirror 228 is disposed at a distance from the second dichroic mirror 226, the third dichroic mirror 228 is disposed on a side of the second dichroic mirror 226 facing away from the first dichroic mirror 225, the third dichroic mirror 228 is configured to reflect a portion of the second mixed light beam 232 to form the second light beam 212, direct the second light beam 212 toward the second camera 22, and transmit another portion of the second mixed light beam 232 to form the third light beam 213.

In this way, by mounting the third dichroic mirror 228 on the stage 70, the third dichroic mirror 228 may be fixed, so that the third dichroic mirror 228 may stably split the second mixed light beam 232 into the second light beam 212 and the third light beam 213, while the third dichroic mirror 228 may be mounted on the stage 70, so as to achieve the splitting of the first mixed light beam 231 and make the split light beam propagate on the stage 701, and further, the second camera 22 and the third camera 23 may be disposed at different positions of the stage 70, thereby improving the space utilization of the optical imaging apparatus 100 in the horizontal direction, and further making the structure of the optical imaging apparatus 100 more compact.

Specifically, the third dichroic mirror 228 may be fixed to the stage 70 by a fastener such as a bolt, and in some embodiments, the third dichroic mirror 228 is disposed perpendicular to the second dichroic mirror 226 and parallel to the first dichroic mirror 225.

Referring to fig. 9 and 11, in some embodiments, the third dichroic mirror 228 is disposed within the first support 71, and the first support 71 is formed with a second light channel 713 between the third dichroic mirror 228 and the second camera 22.

In this manner, second light beam 212, formed by third dichroic mirror 228 reflecting second mixed light beam 232, may propagate through second light channel 713 to second camera 22 for imaging.

Specifically, a second through hole 714 is formed in the side plate of the first support 71 away from the first mirror 227, and the second light beam 212 may propagate from the third dichroic mirror 228 to the second camera 22 through the second through hole 714.

Referring to fig. 5 and 9, in some embodiments, the third light splitting mechanism 223 includes a second mirror 229 mounted on the stage 70, the second mirror 229 being spaced apart from the third dichroic mirror 228, the second mirror 229 being disposed on a side of the third dichroic mirror 228 facing away from the second dichroic mirror 226, the second mirror 229 being configured to reflect the third light beam 213 toward the third camera 23.

In this way, the second mirror 229 can be fixed by mounting the second mirror 229 on the stage 70, so that the second mirror 229 can stably reflect the third light beam 213 toward the third camera 23, improving the imaging stability of the third camera 23. Meanwhile, the second reflecting mirror 229 is mounted on the carrying platform 70, so that the third light beam 213 can be turned and spread on the table 701, the space utilization rate of the optical imaging device 100 in the horizontal direction is improved, and the structure of the optical imaging device 100 is more compact.

Specifically, the second mirror 229 may be fixed to the carrier 70 by a fastener such as a bolt, and in some embodiments, the second mirror 229 is disposed parallel to the third dichroic mirror 228, the second light beam 212 and the third light beam 213 are in the same direction, and the second camera 22 and the third camera 23 are located on the same side of the first support 71; in other embodiments, the second mirror 229 is disposed perpendicular to the third dichroic mirror 228, and the second light beam 212 and the third light beam 213 are opposite in direction, and the second camera 22 and the third camera 23 are respectively located at two sides of the first support 71.

Referring to fig. 10, in some embodiments, the second mirror 229 is disposed in the first support base 71, and the first support base 71 is formed with a third optical channel 715 between the second mirror 229 and the third camera 23.

In this way, the third light beam 213 may propagate to the third camera 23 for imaging through the third light channel 715 after being reflected by the second mirror 229.

Specifically, the side plate of the first support 71 away from the first mirror 227 is provided with a third through hole 716, and the third light beam 213 may propagate from the second mirror 229 to the third camera 23 through the third through hole 716.

Referring to fig. 1, 5 and 6, in some embodiments, the fourth light splitting mechanism 224 includes a third mirror 230 mounted on the second support 76, the third mirror 230 being disposed on the optical axis 15 of the objective lens 10 and inclined with respect to the optical axis 15 of the objective lens 10, the third mirror 230 being located on a side of the first dichroic mirror 225 away from the objective lens 10, the third mirror 230 being configured to reflect the fourth light beam 214 toward the fourth camera 24.

In this way, the third mirror 230 may be fixed by mounting the third mirror 230 on the second support 76, so that the third mirror 230 may stably reflect the fourth light beam 214 toward the fourth camera 24, thereby improving the imaging stability of the fourth camera 24. Meanwhile, the third reflecting mirror 230 is mounted on the second supporting seat 76, so that the propagation of the fourth light beam 214 in the vertical direction can be changed into the propagation in the horizontal direction, the fourth camera 24 can be arranged in the vertical direction relative to the bearing table 70, the space required by the optical imaging device 100 in the horizontal direction is reduced, the space utilization rate of the optical imaging device 100 in the horizontal direction is improved, and the structure of the optical imaging device 100 is more compact.

Specifically, the third mirror 230 may be fixed to the upper surface of the housing of the second support 76 by a fastener such as a bolt, and in some embodiments, the third mirror 230 is disposed parallel or perpendicular to the first dichroic mirror 225.

Referring to fig. 1 and 5, in some embodiments, the optical imaging apparatus 100 includes a first barrel 31, a second barrel 32, a third barrel 33, and a fourth barrel 34, where the first barrel 31, the second barrel 32, and the third barrel 33 are all mounted on the stage 70, the first barrel 31 is disposed on the object side of the first camera 21 and is used to converge the first light beam 211 on the first camera 21, the second barrel 32 is disposed on the object side of the second camera 22 and is used to converge the second light beam 212 on the second camera 22, the third barrel 33 is disposed on the object side of the third camera 23 and is used to converge the third light beam 213 on the third camera 23, the fourth barrel 34 is mounted on the first support 71, and the fourth barrel 34 is disposed on the object side of the fourth camera 24 and is used to converge the fourth light beam 214 on the fourth camera 24.

As such, the first barrel 31 may cooperate with the objective lens 10 to image the first light beam 211 onto the first camera 21 located on the stage 70, the second barrel 32 may cooperate with the objective lens 10 to image the second light beam 212 onto the second camera 22 located on the stage 70, the third barrel 33 may cooperate with the objective lens 10 to image the third light beam 213 onto the third camera 23 located on the stage 70, and the fourth barrel 34 may cooperate with the objective lens 10 to image the fourth light beam 214 onto the fourth camera 24 located on the first support 71.

Specifically, in some embodiments, the first mounting member 702, the second mounting member 703 and the third mounting member 704 are disposed on the carrying platform 70, the fourth mounting member 705 is disposed on the first supporting seat 71, the first mounting member 702, the second mounting member 703, the third mounting member 704 and the fourth mounting member 705 may be formed by a base and an upper cover, the base is fixed on the carrying platform 70 by fastening members such as bolts, the upper cover is fixed on the base by fastening members such as bolts, the base and the upper cover are formed with a circular accommodating space, and the first barrel mirror 31, the second barrel mirror 32, the third barrel mirror 33 and the fourth barrel mirror 34 are respectively placed in the accommodating spaces of the first mounting member 702, the second mounting member 703, the third mounting member 704 and the fourth mounting member 705. The focal lengths of the first barrel 31, the second barrel 32, the third barrel 33, and the fourth barrel 34 may be set according to imaging requirements.

Referring to fig. 5, in some embodiments, the optical imaging apparatus 100 includes a first filter 41, a second filter 42, a third filter 43, and a fourth filter 44, where the first filter 41 is disposed on a side of the first barrel 31 facing away from the first camera 21, and is used to filter the first light beam 211 entering the first barrel 31; a second filter 42 is disposed on a side of the second barrel 32 facing away from the second camera 22 and is configured to filter the second light beam 212 entering the second barrel 32; the third filter 43 is disposed on a side of the third barrel lens 33 facing away from the third camera 23, and is configured to filter the third light beam 213 entering the third barrel lens 33; the fourth filter 44 is disposed on a side of the fourth third barrel 34 facing away from the fourth camera 24 and is configured to filter the fourth light beam 214 entering the fourth third barrel 34. Therefore, the optical filter can filter light, allow fluorescence wave bands to penetrate, cut off unnecessary laser wave bands, and accordingly improve imaging quality of the camera.

Specifically, the first filter 41 and the first barrel mirror 31 are vertically disposed on the optical path of the first light beam 211 reflected by the first mirror 227, respectively, and the first light beam 211 is filtered by the first filter 41 and then converged to the first camera 21 by the first barrel mirror 31.

The second filter 42 and the second barrel lens 32 are vertically disposed on the optical path of the second light beam 212 reflected by the third dichroic mirror 228, and the second light beam 212 is filtered by the second filter 42 and then converged by the second barrel lens 32 to the second camera 22.

The third filter 43 and the third cylindrical mirror 33 are vertically disposed on the optical path of the third light beam 213 reflected by the second mirror 229, respectively, and the third light beam 213 is filtered by the third filter 43 and then converged by the third cylindrical mirror 33 to the third camera 23.

The fourth filter 44 and the fourth third cylindrical mirror 34 are vertically disposed on the optical path of the fourth light beam 214 reflected by the third mirror 230, and the fourth light beam 214 is filtered by the fourth filter 44 and then converged by the fourth third cylindrical mirror 34 to the fourth camera 24.

Referring to fig. 3 and 12, in some embodiments, the optical imaging apparatus 100 includes a second support 76 and a light source assembly 50, the second support 76 is mounted on the carrier 70, the light source assembly 50 is mounted on the second support 76 and disposed on the optical axis 15 side of the objective lens 10, and the light source assembly 50 is used to emit an excitation light beam toward a sample to be measured through the objective lens 10.

In this way, the light source assembly 50 can be fixed to the optical axis 15 side of the objective lens 10 by the second support 76, so that the light source assembly 50 can stably emit the excitation light beam while reducing the distance between the light source assembly 50 and the objective lens 10, resulting in a compact structure of the optical imaging apparatus 100.

Specifically, the light source assembly 50 is fixed to the side of the housing of the second support 76 by fasteners such as bolts. Referring to fig. 5, in some embodiments, the light source assembly 50 includes a first light source 51 and a first lens 52 disposed on an illumination path of the first light source 51, the first lens 52 for controlling a beam aperture of the first light source 51 into the objective lens 10. The first lens 52 is adjusted to control the aperture of the first light source 51 entering the objective lens 10, so that the structure of the optical imaging system is simplified, the debugging difficulty of the optical imaging system is reduced, the light transmittance is improved, the illumination light beam can uniformly illuminate the whole view field, and the excitation efficiency and the imaging quality are improved. The first light source 51 is configured to emit an illumination beam, and when the illumination beam irradiates the sample to be measured, the sample to be measured is excited to generate fluorescence. The first light source 51 emits a light beam of at least one wavelength to excite fluorophores on the plurality of bases to effect sequencing of the plurality of bases.

A first lens 52 may be provided on the illumination light path of the first light source 51, the first lens 52 controlling the beam aperture of the illumination beam entering the objective lens 10. Specifically, the distance between the first lens 52 and the objective 10130 can be adjusted to control the beam caliber of the illumination beam entering the objective lens 10, so that the illumination beam completely enters the objective lens 10 and is projected on the sample to be measured through the objective lens 10, and the sample to be measured is excited to generate fluorescence. In some embodiments, the first lens 52 is an aspheric lens.

Referring to fig. 5, in some embodiments, a bandpass filter 53 is disposed between the first lens 52 and the objective lens 10, and the bandpass filter 53 is used to bandpass filter the light passing through the first lens 52.

The illumination light beam enters the objective lens 10 after bandpass filtering the light source passing through the first lens 52 through the bandpass filter 53 arranged between the first lens 52 and the objective lens 10, thereby removing stray light in the illumination light, and being beneficial to further improving the excitation efficiency and the imaging quality. In some embodiments, the bandpass filter 53 may be a dual bandpass laser filter.

Referring to fig. 5, in some embodiments, a second lens 61 is disposed between the bandpass filter 53 and the objective lens 10, the second lens 61 is disposed on the optical axis 15 of the objective lens 10 and is disposed obliquely with respect to the optical axis 15 of the objective lens 10, and the second lens 61 is configured to reflect the light passing through the bandpass filter 53 to the objective lens 10 and to transmit the light from the objective lens 10 into the spectroscopic mechanism 20.

The second lens 61 reflects the illumination beam emitted by the first light source 51 passing through the band-pass filter 53 to the objective lens 10, so that the illumination beam emitted by the first light source 51 irradiates the sample to be measured after passing through the objective lens 10, and excites the sample to be measured to generate fluorescence with multiple wavelengths.

Specifically, the second lens 61 is disposed on the optical axis 15 of the objective lens 10 and is disposed at an angle of 45 ° with respect to the optical axis 15 of the objective lens 10, and an angle of 90 ° is formed between the second lens 61 and the first dichroic mirror 225, so that the objective lens 10 and the first light source 51 are disposed on the same side of the second lens 61, and the first light source 51 is incident on the objective lens 10. In some embodiments, the second lens 61 is a dichroic mirror.

Referring to fig. 3 and 5, in some embodiments, the optical imaging apparatus 100 further includes a fifth stand 77 and an auto-focusing device 60 mounted on the fifth stand 77, the fifth stand 77 is mounted on the carrying platform 70, the auto-focusing device 60 includes a second light source 62 and a focusing sensor 63, and the second light source 62 is used for projecting a light beam emitted by the second light source 62 onto a sample to be measured through the objective lens 10; the focusing sensor 63 is used for receiving the light beam reflected from the sample to be measured and collimated by the objective lens 10, and converting the light signal into an electrical signal, so that the objective lens 10 moves according to the electrical signal, and the plane of the sample to be measured is located at the focal plane of the objective lens 10.

The automatic focusing device 60 is mainly used for marking the object distance after the objective lens 10 finishes focusing, monitoring the change amount of the object distance in real time in the gene sequencing process, correcting the change amount, and fixing the automatic focusing device 60 through the fifth bracket 77 to ensure that the objective lens 10 is always focused clearly, ensure the accuracy of the acquired image of the detection camera, and finally obtain a clear image within the focal depth range.

Specifically, the fifth bracket 77 may have an "i" shape and a hollow structure, and the bottom of the fifth bracket 77 is fixed to the carrying platform 70 by a fastener such as a bolt, and the autofocus device 60 is fixed to the surface of the fifth bracket 77 by a fastener such as a bolt.

The focusing sensor 63 is configured to receive a light beam reflected from a sample to be measured and collimated by the objective lens 10, determine a distance between a plane of the sample to be measured and a focal plane of the objective lens 10 according to the light beam, and convert an optical signal into an electrical signal, where the electrical signal is used to characterize the distance between the plane of the sample to be measured and the focal plane of the objective lens 10, and adjust the plane of the sample to be measured by using the electrical signal, so that the plane of the sample to be measured is located at the focal plane position of the objective lens 10.

Referring to fig. 5, in some embodiments, the auto-focusing device 60 further includes a fourth dichroic mirror 64, the fourth dichroic mirror 64 is disposed on the optical axis 15 of the objective lens 10, the fourth dichroic mirror 64 is configured to reflect the light beam emitted by the second light source 62, so that the light beam emitted by the second light source 62 sequentially passes through the objective lens 10 to be projected onto the sample to be measured, and reflects the light beam reflected from the sample to be measured to the focusing sensor 63, and the fourth dichroic mirror 64 is further configured to transmit the fourth light beam 214.

In this way, the light paths are arranged by fully utilizing the reflectivity and the light transmittance of the fourth dichroic mirror 64, so that the space utilization rate is improved, and the structure is more compact.

Specifically, the fourth dichroic mirror 64 is disposed on the optical axis 15 of the objective lens 10, and the fourth dichroic mirror 64 is disposed in parallel with the first dichroic mirror 225. The light beam emitted by the second light source 62 in the automatic focusing device 60 is reflected to the first dichroic mirror 225 through the fourth dichroic mirror 64, transmitted to the second lens 61 through the first dichroic mirror 225, transmitted to the objective lens 10 through the second lens 61, converged on the gene sequencing fluorescent biochip through the objective lens 10, and returned to the automatic focusing device 60 according to the original light path.

The genetic sequencing apparatus of the embodiment of the present utility model includes an optical imaging device 100.

In this way, the optical imaging device 100 adopts a double-layer structure through horizontal layout, so that the space utilization rate of the optical imaging device 100 in the horizontal direction is higher, the structure of the optical imaging device 100 is more compact, and the structure of the gene sequencing equipment is more compact.

The sequencing device of the present utility model includes, but is not limited to, devices having a sequencing function such as a gene sequencing device, a nucleic acid sequencing device, etc., and other systems or devices manufactured using the same principle are also applicable to the present utility model.

In the description of the present specification, reference to the terms "one embodiment," "certain embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the utility model. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiments or examples. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

While embodiments of the present utility model have been shown and described, it will be understood by those of ordinary skill in the art that: many changes, modifications, substitutions and variations may be made to the embodiments without departing from the spirit and principles of the utility model, the scope of which is defined by the claims and their equivalents.

Claims (17)

1. An optical imaging apparatus, comprising:

A carrying platform;

An objective lens mounted on the stage;

A beam splitting mechanism mounted on the stage, the beam splitting mechanism for splitting light from the objective lens into different first, second, third and fourth beams; and

A first camera, a second camera, a third camera and a fourth camera, wherein the first camera is positioned on the optical path of the first light beam and is used for receiving the first light beam and forming an image, the second camera is positioned on the optical path of the second light beam and is used for receiving the second light beam and forming an image, the third camera is positioned on the optical path of the third light beam and is used for receiving the third light beam and forming an image, and the fourth camera is positioned on the optical path of the fourth light beam and is used for receiving the fourth light beam and forming an image;

the first camera, the second camera and the third camera are mounted on the bearing table;

The first supporting seat is installed on the bearing table, the fourth camera is installed on the first supporting seat, the distance between any one of the first camera, the second camera and the third camera and the bearing table is a first distance, the distance between the fourth camera and the bearing table is a second distance, and the second distance is larger than the first distance.

2. The optical imaging apparatus of claim 1, wherein a table top of the stage is perpendicular to an optical axis of the objective lens.

3. The optical imaging apparatus of claim 1, wherein the first camera and the second camera are located on opposite sides of the first mount, respectively.

4. The optical imaging apparatus of claim 1, wherein the optical imaging apparatus comprises a first mount fixed to the stage, the first camera being fixed to the first mount; and/or the number of the groups of groups,

The optical imaging device comprises a second bracket fixed on the bearing table, and the second camera is fixed on the second bracket; and/or the number of the groups of groups,

The optical imaging device comprises a third bracket fixed on the bearing table, and the third camera is fixed on the third bracket; and/or the number of the groups of groups,

The optical imaging device comprises a fourth bracket fixed on the first supporting seat, and the fourth camera is fixed on the fourth bracket.

5. The optical imaging apparatus of claim 1, wherein the light splitting mechanism comprises a first light splitting mechanism, a second light splitting mechanism, a third light splitting mechanism, and a fourth light splitting mechanism, wherein,

The first light splitting mechanism splits the light from the objective lens to form a first mixed light beam and the fourth light beam, and is further used for splitting a part of the first mixed light beam to form the first light beam, converging the first light beam to the first camera, and transmitting the other part of the first mixed light beam as a second mixed light beam to the second light splitting mechanism;

The second light splitting mechanism is used for receiving the second mixed light beam, splitting the second mixed light beam to form the second light beam and the third light beam, converging the second light beam to the second camera, and transmitting the third light beam to the third light splitting mechanism;

the third light splitting mechanism is used for converging the third light beam to the third camera;

The fourth light splitting mechanism receives the fourth light beam formed by the first light splitting mechanism and converges the fourth light beam to the fourth camera.

6. The optical imaging apparatus according to claim 5, comprising a second support mounted on the stage, the second support being juxtaposed with the first support, the first light splitting mechanism comprising a first dichroic mirror disposed on the optical axis of the objective lens and mounted on the second support and disposed obliquely with respect to the optical axis of the objective lens, and a second dichroic mirror for reflecting light from the objective lens to form the first mixed light beam and transmitting light from the objective lens to form the fourth light beam;

The second dichroic mirror is arranged on the bearing table and is arranged at a distance from the first dichroic mirror, and the second dichroic mirror is used for reflecting a part of the first mixed light beam to form the first light beam and directing the first light beam to the first camera; and transmits another portion of the first beam to form the second mixed beam.

7. The optical imaging arrangement of claim 6, wherein the first light splitting mechanism further comprises a first mirror mounted on the stage, the first mirror being located on one side of the first support and spaced from the second dichroic mirror, the first mirror being configured to reflect a portion of the first light beam from the second dichroic mirror toward the first camera.

8. The optical imaging arrangement of claim 7, wherein the second dichroic mirror is at least partially disposed within the first support, the first support being formed with a first light channel between the second dichroic mirror and the first mirror.

9. The optical imaging arrangement of claim 8, wherein the second dichroic mechanism includes a third dichroic mirror mounted on the stage, the third dichroic mirror being spaced apart from the second dichroic mirror, the third dichroic mirror being disposed on a side of the second dichroic mirror facing away from the first dichroic mirror, the third dichroic mirror being configured to reflect a portion of the second mixed light beam to form the second light beam, direct the second light beam to the second camera, and transmit another portion of the second mixed light beam to form the third light beam.

10. The optical imaging arrangement of claim 9, wherein the third dichroic mirror is disposed within the first mount, the first mount being formed with a second light channel between the third dichroic mirror and the second camera.

11. The optical imaging arrangement according to claim 9, characterized in that the third light splitting mechanism comprises a second mirror mounted on the carrier, the second mirror being arranged at a distance from the third dichroic mirror, the second mirror being arranged at a side of the third dichroic mirror facing away from the second dichroic mirror, the second mirror being arranged to reflect the third light beam towards the third camera.

12. The optical imaging arrangement of claim 11, wherein the second mirror is disposed within the first mount, the first mount being formed with a third optical channel between the second mirror and the third camera.

13. The optical imaging apparatus according to claim 6, wherein the fourth light-splitting mechanism includes a third mirror mounted on the second support, the third mirror being disposed on the optical axis of the objective lens and inclined with respect to the optical axis of the objective lens, the third mirror being located on a side of the first dichroic mirror away from the objective lens, the third mirror being for reflecting the fourth light beam toward the fourth camera.

14. The optical imaging apparatus according to claim 1, wherein the optical imaging apparatus includes a first barrel lens, a second barrel lens, a third barrel lens, and a fourth barrel lens, each of which is mounted on the mount, the first barrel lens being disposed on an object side of the first camera and configured to converge the first light beam onto the first camera, the second barrel lens being disposed on an object side of the second camera and configured to converge the second light beam onto the second camera, the third barrel lens being disposed on an object side of the third camera and configured to converge the third light beam onto the third camera, the fourth barrel lens being mounted on the first mount, the fourth barrel lens being disposed on an object side of the fourth camera and configured to converge the fourth light beam onto the fourth camera.

15. The optical imaging apparatus according to claim 1, wherein the optical imaging apparatus comprises a second support base mounted on the carrying table, and a light source assembly mounted on the second support base and disposed on a side of an optical axis of the objective lens, the light source assembly being configured to emit an excitation light beam toward a sample to be measured through the objective lens.

16. The optical imaging apparatus according to claim 1, further comprising a fifth mount mounted on the stage and an autofocus device mounted on the fifth mount, the autofocus device comprising a second light source for projecting a light beam emitted by the second light source onto a sample to be measured via the objective lens and a focus sensor; the focusing sensor is used for receiving the light beam reflected from the sample to be detected and collimated by the objective lens, and converting the light signal into an electric signal so that the objective lens moves according to the electric signal, and the plane of the sample to be detected is positioned at the focal plane of the objective lens.

17. A genetic sequencing apparatus comprising the optical imaging device of any one of claims 1-16.