CN219512087U - Multi-wavelength liquid detection device based on light splitting technology - Google Patents

Multi-wavelength liquid detection device based on light splitting technology Download PDF

Info

Publication number
CN219512087U
CN219512087U CN202320837851.6U CN202320837851U CN219512087U CN 219512087 U CN219512087 U CN 219512087U CN 202320837851 U CN202320837851 U CN 202320837851U CN 219512087 U CN219512087 U CN 219512087U
Authority
CN
China
Prior art keywords
light
detection
spectroscopic
optical fiber
unit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202320837851.6U
Other languages
Chinese (zh)
Inventor
陈思
胡军荣
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hangzhou Zhunxin Biotechnology Co ltd
Original Assignee
Hangzhou Zhunxin Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hangzhou Zhunxin Biotechnology Co ltd filed Critical Hangzhou Zhunxin Biotechnology Co ltd
Priority to CN202320837851.6U priority Critical patent/CN219512087U/en
Application granted granted Critical
Publication of CN219512087U publication Critical patent/CN219512087U/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A20/00Water conservation; Efficient water supply; Efficient water use
    • Y02A20/20Controlling water pollution; Waste water treatment

Landscapes

  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The utility model provides a multi-wavelength liquid detection device based on a spectroscopic technology, and relates to the technical field of PCR detection. The utility model provides a multi-wavelength liquid detection device based on a light splitting technology, which comprises a light source, a light combining unit, a detection pool and a light splitting detection unit, wherein the light combining unit is arranged on the light source; the light source comprises a plurality of light sources, the light sources are used for emitting light beams with different central wavelengths, the light beams emitted by the light sources are combined into combined light through the light combining unit, the combined light irradiates sample solution in the detection pool to be stimulated to form measuring light, and the measuring light is incident into the light splitting detection unit to carry out liquid detection. The multi-wavelength liquid detection device based on the light splitting technology is simple in structure, the production and detection cost of the device is reduced, the detection process is stable and reliable, and the reliability of the device is improved.

Description

Multi-wavelength liquid detection device based on light splitting technology
Technical Field
The utility model relates to the technical field of PCR detection, in particular to a multi-wavelength liquid detection device based on a spectroscopic technology.
Background
In genetic engineering, the working principle of a PCR detection device is that reactants are subjected to temperature circulation between a specific denaturation temperature, a specific renaturation temperature and a specific extension temperature, and target DNA is amplified in millions; meanwhile, the test tube is irradiated by using excitation light with different wavelengths (multi-excitation) or excitation light with a single wavelength (single excitation), and when the reagent in the test tube is excited to emit fluorescence, a fluorescence intensity signal is acquired through the optical sensor and is transmitted to a computer for real-time data analysis.
In the existing PCR detection technology, a CCD camera system is often adopted for detection, and the fluorescence intensity of each PCR reaction is subjected to image processing, but the detection mode can cause quite complex processing process due to the change of the fluorescence intensity and the difference of brightness of images, and meanwhile, the instrument is also quite expensive; in addition, there is also a method of scanning detection by rotating the PCR tube and then using a stationary fluorescence detection system, but since the reactant in the PCR tube is centrifuged back at a high speed, the emitted fluorescence becomes very complicated and unstable in a dynamic state. Therefore, a detection device with simple structure, stability, reliability and low cost is needed to solve the above-mentioned problems.
Disclosure of Invention
The utility model provides a multi-wavelength liquid detection device based on a light splitting technology, and aims to solve the problems of complex detection process, poor stability and higher cost of the existing detection device in the prior art.
Embodiments of the present utility model are implemented as follows:
in one aspect of the embodiments of the present utility model, a multi-wavelength liquid detection device based on a spectroscopic technique is provided Comprises a light source, a photosynthetic beam unit, a detection pool and a spectroscopic detection unit;
the light source comprises a plurality of light sources, the light sources are used for emitting light beams with different central wavelengths, the light beams emitted by the light sources are combined into combined light through the light combining unit, the combined light irradiates sample solution in the detection pool to be stimulated to form measuring light, and the measuring light is incident into the light splitting detection unit to carry out liquid detection.
Optionally, the light combining unit includes a first focusing lens, a first optical filter device, and a light combining lens; the first focusing lens is used for focusing the light beams emitted by the light source, the first filter device is used for filtering the light beams with non-central wavelengths, and the light converging lens is used for converging different light beams into combined light; the first focusing lens, the first optical filter device and the light converging lens are sequentially arranged along the emergent direction of the light beam.
Optionally, the beam splitting detection unit comprises a plurality of beam splitting detection modules, the beam splitting detection modules comprise beam splitting mirrors, a second optical filter device and a detector, and the beam splitting mirrors are used for transmitting the measuring light emitted by the detection pool; the second filter device is used for filtering out measuring light with different wavelengths, and the detector is used for receiving the measuring light.
Optionally, the spectroscopic detection module further includes a second converging lens disposed between the second filter device and the detector, so as to converge the measurement light filtered by the second filter device and inject the measurement light into the detector.
Optionally, the beam splitter employs a transflector and/or a reflector.
Optionally, the multi-wavelength liquid detection device based on the spectroscopic technology further comprises a first optical fiber and a second optical fiber;
the first optical fiber is arranged between the light combining unit and the detection pool so that the combined light is coupled into the first optical fiber and transmitted to the detection pool; the second optical fiber is arranged between the detection cell and the spectroscopic detection unit, so that the measurement light is coupled into the second optical fiber and transmitted to the spectroscopic detection unit.
Optionally, the multi-wavelength liquid detection device based on the spectroscopic technique further comprises a third converging lens and a fourth converging lens;
the third converging lens is arranged between the light combining unit and the first optical fiber so that the combined light passes through the third converging lens and is coupled into the first optical fiber; the fourth converging lens is arranged between the second optical fiber and the light splitting detection unit, so that the measuring light passes through the fourth converging lens and then is emitted into the light splitting detection unit.
Optionally, a reflection enhancing film is plated on the surface of the beam splitter.
Optionally, the number of the detection cells is two or more, and the number of the spectroscopic detection units is correspondingly equal to the number of the detection cells.
Optionally, the light source is an LED.
The beneficial effects of the embodiment of the utility model include: the embodiment of the utility model provides a multi-wavelength liquid detection device based on a light splitting technology Comprises a light source, a photosynthetic beam unit, a detection pool and a spectroscopic detection unit; the light source comprises a plurality of light sources, the light sources are used for emitting a plurality of light beams with different central wavelengths and combining the light beams into combined light through the light combining unit, the light combining unit can filter out the light with non-central wavelengths of the light beams and combine the light with different wavelengths into combined light, the requirement of actual detection on the light with various wavelengths is met through simple operation, and the detection reliability is improved; the beam combination light irradiates the detection pond, fluorescence excitation is carried out on the sample solution in the detection pond, measuring light with different wavelengths is excited, the measuring light is incident into the light splitting detection unit, the measuring light with different wavelengths is filtered out in sequence, liquid detection is realized, the detection process is simple and convenient, meanwhile, the measuring light with different wavelengths is not affected, and the detection accuracy and reliability are improved. The multi-wavelength liquid detection device based on the light splitting technology is simple in structure, the production and detection cost of the device is reduced, the detection process is stable and reliable, and the reliability of the device is improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a multi-wavelength liquid detection device based on spectroscopic techniques according to an embodiment of the present utility model;
FIG. 2 is a schematic diagram of a photosynthetic beam unit provided by an embodiment of the present utility model;
FIG. 3 is a schematic diagram of a spectroscopic detection unit according to an embodiment of the present utility model;
FIG. 4 is a schematic diagram of a plurality of detection cells according to an embodiment of the present utility model.
Icon: 100-a multi-wavelength liquid detection device based on a spectroscopic technique; 110-a light source; a 120-photosynthetic beam unit; 121-a light combining module; 1211-a first converging lens; 1212-a first filter device; 130-a light combining mirror; 140-a detection cell; 150-a spectroscopic detection unit; 151-spectroscopic detection module; 1511-beam splitters; 1512-a second filter device; 153-detector; 160-a first optical fiber; 170-a second optical fiber; 180-a third converging lens; 190-fourth converging lens.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some, but not all, embodiments of the present utility model. The components of the embodiments of the present utility model generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
In the description of the present utility model, it should be understood that the terms "orientation" or "positional relationship" are based on the orientation or positional relationship shown in the drawings, and are merely for convenience of description and to simplify the description, rather than to indicate or imply that the apparatus or elements referred to must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as limiting the utility model.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
Referring to fig. 1, in one aspect of the present utility model, a multi-wavelength liquid detection device 100 based on a spectroscopic technique is provided Comprises a light source 110, a photosynthetic beam unit 120, a detection cell 140 and a spectroscopic detection unit 150;
the light sources 110 include a plurality of light sources 110 for emitting light beams with different center wavelengths, and the light beams are combined into combined light by the beam combining unit 120, and the combined light irradiates the sample solution in the detection cell 140 to be stimulated to form measurement light, and the measurement light is arranged in the beam splitting detection unit 150 to perform liquid detection.
Specifically, in a preferred scheme of the present embodiment, as shown in fig. 1, four LEDs are adopted for the light sources 110, and central wavelengths of light beams emitted by the four light sources 110 are different; the device further comprises a light combining unit 120, after the light sources 110 emitted by the light sources 110 enter the light combining unit 120, the light sources 110 with non-central wavelengths are filtered by the light combining unit 120 and combined into combined light with four different central wavelengths, the combined light irradiates the detection cell 140, and excites sample solution in the cell, so that the liquid is excited to generate four fluorescent measurement lights which have different central wavelengths respectively and are incident into the light splitting detection unit 150, and the light beams with the four different central wavelengths in the measurement lights are sequentially filtered and split, and are received and detected.
It should be noted that, in the first embodiment of the present utility model, as shown in fig. 2, the light combining unit 120 includes a plurality of first focusing lenses 1211, a plurality of first filtering devices 1212 and a plurality of light combining lenses 130, each of the first focusing lenses 1211, the first filtering devices 1212 and the light combining lenses 130 respectively form a light combining module 121, so that the light combining unit includes a plurality of light combining modules 121, each of the light combining modules 121 individually corresponds to a light beam emitted by one of the light sources 110, and respectively processes the corresponding light beams, and finally combines the light beams into a combined light beam; by the arrangement, all light beams in the detection process are not interfered with each other, and the reliability of the detection process is improved.
In the second embodiment of the present utility model, as shown in fig. 3, the spectroscopic detection unit 150 includes a plurality of spectroscopic detection modules 151, and the measurement light includes a plurality of light beams with different wavelengths, and after the light beams enter the spectroscopic detection modules 151, the plurality of spectroscopic detection modules 151 respectively correspond to the measurement light with one single wavelength filtered by the measurement light, and are respectively received and detected. The arrangement can enable the beam splitting treatment in the detection process to be free from interference and influence, and improves the reliability and accuracy of the detection process.
The multi-wavelength liquid detection device based on the light splitting technology provided by the embodiment of the utility model comprises a light source, a light combining unit, a detection pool and a light splitting detection unit; the light source comprises a plurality of light sources, the light sources are used for emitting a plurality of light beams with different central wavelengths and combining the light beams into combined light through the light combining unit, the light combining unit can filter out the light with non-central wavelengths of the light beams and combine the light with different wavelengths into combined light, the requirement of actual detection on the light with various wavelengths is met through simple operation, and the detection reliability is improved; the beam combination light irradiates the detection pond, fluorescence excitation is carried out on the sample solution in the detection pond, measuring light with different wavelengths is excited, the measuring light is incident into the light splitting detection unit, the measuring light with different wavelengths is filtered out in sequence, liquid detection is realized, the detection process is simple and convenient, meanwhile, the measuring light with different wavelengths is not affected, and the detection accuracy and reliability are improved. The multi-wavelength liquid detection device based on the light splitting technology is simple in structure, the production and detection cost of the device is reduced, the detection process is stable and reliable, and the reliability of the device is improved.
In one implementation of the present utility model, as shown in fig. 1, the light combining unit 120 includes a first condensing lens 1211, a first filter device 1212, and a light combining lens 130; the first focusing lens 1211 is used for focusing the light beam emitted by the light source 110, the first filter 1212 is used for filtering the light beam with non-central wavelength, and the light combiner 130 is used for combining different light beams into combined light; the first condensing lens 1211, the first filter device 1212, and the combiner 130 are sequentially disposed along the outgoing direction of the light beam.
Specifically, as shown in fig. 2, the light combining unit 120 has a plurality of first condensing lenses 1211, a plurality of first light filtering devices 1212, and a plurality of light combining lenses 130; each of the first focusing lens 1211, the first filter and the light combining lens 130 respectively form a light combining module 121, so that the light combining unit includes a plurality of light combining modules 121, and the plurality of light combining modules 121 are arranged in parallel and respectively correspond to the plurality of light sources 110. When the light sources 110 emit light beams with different wavelengths, each light beam is respectively emitted into a corresponding light combining module 121 in the light combining unit 120, and sequentially emitted into the first converging lens 1211, the first optical filter 1212 and the light combining lens 130 and emitted out of the light combining unit 120, wherein the first converging lens 1211 is an aspheric lens; in a preferred embodiment of the present utility model, the light converging mirror 130 in the first light converging module 121 uses a transparent mirror and a reflecting mirror to reflect the light beam, and the reflecting surfaces of the transparent mirror and the reflecting mirror are coated with an antireflection film, and the other surface opposite to the reflecting surface is coated with an antireflection film.
By setting the light combining unit 120 as a plurality of light combining modules 121, each light beam in the detection process is not interfered with each other, and the reliability of the detection process is improved; the arrangement of the first condensing lens 1211 can improve the light receiving efficiency of the device; the reflection enhancing film and the reflection enhancing film on the reflection mirror can better transmit and reflect light beams so as to combine a plurality of light beams with different wavelengths into combined light beams, thereby improving the utilization rate of the light beams and enabling the detection process to be more efficient and reliable.
In one implementation of the present utility model, as shown in fig. 3, the spectroscopic detection unit 150 includes a plurality of spectroscopic detection modules 151, where the spectroscopic detection modules 151 include a beam splitter 1511, a second filter 1512, and a detector 153, and the beam splitter 1511 is used to transmit the measurement light emitted from the detection cell 140; the second filter 1512 is configured to filter out measurement light of different wavelengths, and the detector 153 is configured to receive the measurement light.
Specifically, the spectroscopic detection unit 150 is composed of a plurality of spectroscopic detection modules 151, and the plurality of spectroscopic detection modules 151 are arranged in parallel; when the detection cell 140 is excited to emit a plurality of fluorescent measurement lights, the measurement lights with different center wavelengths are incident on the spectroscopic detection unit 150 and sequentially enter the spectroscopic detection module 151; the beam-splitting detection module 151 comprises a beam-splitting mirror 1511, a second optical filter 1512 and a detector 153, and the measuring light sequentially enters the beam-splitting detection module 151 and enters the beam-splitting mirror 1511, wherein the beam-splitting mirror 1511 adopts a transparent mirror, the fourth beam-splitting detection module 151 adopts a reflecting mirror, a reflection enhancing film is plated on the reflecting surface of the fourth beam-splitting detection module, and an anti-reflection film is plated on the other surface opposite to the reflecting surface. The light beams enter the second filter after passing through the beam splitter 1511 to filter out the measurement light of different wavelengths, and converge it to the detector 153 to receive and detect the light beams.
By arranging the light splitting detection unit 150, each light beam in the detection process can be split, the influence of mutual interference is avoided, and the reliability and the accuracy of the detection process are improved; the arrangement of the antireflection film and the antireflection film on the reflection mirror can better transmit and reflect light beams, so that the utilization rate of the light beams is improved, and the detection process is more efficient and reliable.
As illustrated in fig. 3, the spectroscopic detection module 151 further includes a second condensing lens disposed between the second filter 1512 and the detector 153 to condense the measurement light filtered by the second filter 1512 and incident into the detector 153.
Specifically, the spectroscopic detection module 151 further includes a second converging lens, where the second converging lens is disposed between the second filter 1512 and the detector 153, and when the measurement light passes through the second filter 1512, the measurement light enters the second converging lens first, and is converged by the second converging lens and enters the detector 153.
By arranging the second converging lens, the light receiving efficiency of the device can be improved, and further the reliability and the detection efficiency of the device are improved.
Illustratively, beam splitter 1511 employs a transflector and/or a mirror.
Specifically, the beam splitter 1511 is disposed in the beam splitter detection module 151, where the beam splitter detection module 151 includes a plurality of beam splitters 1511, and the number of beam splitters 1511 corresponds to the number of light sources 110. In one embodiment of the present utility model, the light source 110 includes four LEDs, and thus the beam splitter 151 and the beam splitter 1511 correspondingly include four LEDs. Wherein the first spectroscopic detection module 151, the second spectroscopic detection module 151 and the third spectroscopic detection module 151 are all set as a transflective mirror, and the fourth spectroscopic detection module 151 is a reflective mirror.
By the arrangement of the beam splitter 1511, the manufacturing cost of the device can be reduced, and the device is convenient for production and assembly; the different arrangement of the transparent mirror and the reflecting mirror can be combined with the arrangement position to further improve the transmission and reflection of the light beam so as to improve the reliability of the device and the high efficiency of detection.
Illustratively, as shown in fig. 1, the multi-wavelength liquid detection device 100 based on the spectroscopic technique further includes a first optical fiber 160 and a second optical fiber 170;
the first optical fiber 160 is disposed between the light combining unit 120 and the detection cell 140 such that the combined light is coupled into the first optical fiber 160 and transmitted to the detection cell 140; the second optical fiber 170 is disposed between the detection cell 140 and the spectroscopic detection unit 150, so that the measurement light is coupled into the second optical fiber 170 and transmitted to the spectroscopic detection unit 150.
Specifically, the multi-wavelength liquid detection device 100 based on the spectroscopic technique further has a first optical fiber 160 and a second optical fiber 170; the first optical fiber 160 is disposed between the light combining unit 120 and the detection cell 140, and when the light combining unit 120 combines the light beams with different center wavelengths into a combined light beam, the combined light beam is coupled by the first optical fiber 160 and is transmitted into the detection cell 140; the second optical fiber 170 is disposed between the detection cell 140 and the spectroscopic detection unit 150, and when the detection cell 140 is excited to locate the fluorescence measurement light, the measurement light is coupled by the second optical fiber 170 and transmitted to the spectroscopic detection unit 150 for performing the subsequent spectroscopic detection operation.
By the action of the first optical fiber 160 and the second optical fiber 170, the transmission efficiency of the light beam can be improved, and the stability and reliability of the device can be further improved.
In one implementation of the present utility model, as shown in fig. 1, the multi-wavelength liquid detection apparatus 100 based on the spectroscopic technique further includes a third condensing lens 180 and a fourth condensing lens 190;
the third converging lens 180 is disposed between the light combining unit 120 and the first optical fiber 160, so that the combined light is coupled into the first optical fiber 160 after passing through the third converging lens 180; the fourth condensing lens 190 is disposed between the second optical fiber 170 and the spectroscopic detection unit 150 such that the measurement light is incident into the spectroscopic detection unit 150 after passing through the fourth condensing lens 190.
Specifically, the multi-wavelength liquid detection apparatus 100 based on the spectroscopic technique further has a third condenser lens 180 and a fourth condenser lens 190, and the third condenser lens 180 and the fourth condenser lens 190 are aspherical lenses. The third converging lens 180 is disposed between the light combining unit 120 and the first optical fiber 160, and the combined light emitted from the light combining unit 120 is converged by the third converging lens 180, and then coupled by the first optical fiber 160 and transmitted into the detection cell 140; the fourth converging lens 190 is disposed between the second optical fiber 170 and the spectroscopic detection unit 150, and when the detection cell 140 is excited to locate the fluorescence measurement light, the measurement light is coupled to the fourth converging lens 190 through the second optical fiber 170, and then converged by the fourth converging lens 190, and enters the spectroscopic detection unit 150 for subsequent spectroscopic detection.
By arranging the third condensing lens 180 and the fourth condensing lens 190, the light receiving efficiency can be further improved, so that coupling and transmission are realized by matching the first optical fiber 160 and the second optical fiber 170, and the stability and reliability of the device are further improved.
In one implementation of the present utility model, the surface of beam splitter 1511 is coated with an antireflection film and an antireflection film.
Specifically, the beam splitter 1511 adopts two forms of a reflecting mirror and a reflecting mirror, wherein the reflecting surfaces of the reflecting mirror and the reflecting mirror are plated with an antireflection film, and one surface away from the reflecting surface is plated with an antireflection film. Through the arrangement of the antireflection film and the antireflection film, light beams can be better transmitted and reflected, so that the reliability of the device and the high efficiency of detection are improved.
As illustrated in fig. 4, the number of detection cells 140 is two or more, and the number of spectroscopic detection units 150 is equal to the number of detection cells 140.
Specifically, in another embodiment of the present utility model, the detecting cells 140 include two detecting cells 140, and each detecting cell 140 corresponds to one spectroscopic detecting unit 150, so that the spectroscopic detecting units 150 have two detecting units; the spectrum detection module 151 in the spectrum detection unit 150 is correspondingly divided into eight units; at this time, the light combining unit and the two detecting tanks 140 are both provided with optical fibers. For example, the two detecting tanks 140 are respectively a first detecting tank 140 and a second detecting tank 140, and a first optical fiber 160 is arranged between the light combining unit and the first detecting tank 140, and a third optical fiber is arranged between the light combining unit and the second detecting tank 140; correspondingly, optical fibers are also arranged between the two detection cells 140 and the spectroscopic detection unit 150, namely, a second optical fiber 170 is arranged between the first detection cell 140 and the first spectroscopic detection unit 150, and a fourth optical fiber is arranged between the second detection cell 140 and the second spectroscopic detection unit 150. When the number of the detection cells 140 is two or more, the optical fibers and the spectroscopic detection units 150 are correspondingly increased and cooperatively arranged.
Through the arrangement, simultaneous detection of different samples can be realized, so that the detection time is saved, and the detection efficiency and reliability are improved.
In one implementation of the present embodiment, the light source 110 is an LED.
Specifically, the light source 110 provided by the present device is an LED. The LED can provide stable light beams, and meanwhile, the cost is lower, so that the device can be reduced and the stability of the device can be improved.
The detection process of the multi-wavelength liquid detection device 100 based on the spectroscopic technique provided by the utility model is realized as follows:
the four light sources 110 respectively emit light beams and emit the light beams into the light combining unit 120, the four light beams respectively filter the light with non-central wavelength in the light combining unit 120, the light beams with four different wavelengths are combined into combined light by utilizing the light combining lens 130, and the combined light is coupled into the first optical fiber 160 through the third converging lens 180 and is transmitted to the detection pool 140 along with the first optical fiber 160;
the detection tank 140 stores detection liquid, the detection liquid is excited to have fluorescence measurement light with different wavelengths under the irradiation of the combined beam light, the measurement light is coupled into the second optical fiber 170, and the measurement light is transmitted to the spectroscopic detection unit 150 along with the second optical fiber 170 through the fourth converging lens 190;
the spectroscopic detection unit 150 has four spectroscopic detection modules 151 therein, and measurement light sequentially enters the four spectroscopic detection modules 151, and filters out measurement light of different wavelengths, and is received and detected by the corresponding detectors 153.
The above description is only an example of the present utility model and is not intended to limit the scope of the present utility model, and various modifications and variations will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims (10)

1. The multi-wavelength liquid detection device based on the light splitting technology is characterized by comprising a light source, a light combining unit, a detection pool and a light splitting detection unit;
the light sources comprise a plurality of light sources, and the light sources are used for emitting light beams with different center wavelengths; the light beams emitted by the light sources pass through the light combining unit to be combined into combined light, the combined light irradiates the sample solution in the detection cell to be stimulated to form measuring light, and the measuring light is incident into the light splitting detection unit to carry out liquid detection.
2. The spectroscopic-based multi-wavelength liquid detection device of claim 1, wherein the light combining unit comprises a first converging lens, a first light filtering device, and a light combining lens; the first focusing lens is used for focusing the light beams emitted by the light source, the first filter device is used for filtering the light beams with non-central wavelength, and the light combining lens is used for combining different light beams into combined light; the first focusing lens, the first optical filter device and the light converging lens are sequentially arranged along the emergent direction of the light beam.
3. The spectroscopic technique-based multi-wavelength liquid detection apparatus according to claim 1, wherein the spectroscopic detection unit includes a plurality of spectroscopic detection modules including a beam splitter for transmitting the measurement light emitted from the detection cell, a second filter device, and a detector; the second filter device is used for filtering out the measuring light with different wavelengths, and the detector is used for receiving the measuring light.
4. A spectroscopic-based multi-wavelength liquid detection device as claimed in claim 3, wherein the spectroscopic detection module further comprises a second converging lens disposed between the second filter device and the detector to converge the measurement light filtered by the second filter device and incident into the detector.
5. A multi-wavelength liquid detection apparatus based on spectroscopic technique according to claim 3, wherein the beam splitter employs a transflector and/or a reflector.
6. The spectroscopic-based multi-wavelength liquid detection device of claim 1, further comprising a first optical fiber and a second optical fiber;
the first optical fiber is arranged between the light combining unit and the detection cell so that the combined light is coupled into the first optical fiber and transmitted to the detection cell; the second optical fiber is arranged between the detection cell and the spectroscopic detection unit, so that the measurement light is coupled into the second optical fiber and transmitted to the spectroscopic detection unit.
7. The spectroscopic-based multi-wavelength liquid detection device of claim 6, further comprising a third converging lens and a fourth converging lens;
the third converging lens is arranged between the light combining unit and the first optical fiber so that the combined light passes through the third converging lens and is coupled into the first optical fiber; the fourth converging lens is arranged between the second optical fiber and the light splitting detection unit, so that the measuring light passes through the fourth converging lens and then is emitted into the light splitting detection unit.
8. The spectroscopic-technology-based multi-wavelength liquid detection device according to claim 5, wherein a reflection enhancing film is plated on the surface of the beam splitter.
9. The spectroscopic technique-based multi-wavelength liquid detection device according to claim 1, wherein the number of the detection cells is two or more, and the number of the spectroscopic detection units is correspondingly equal to the number of the detection cells.
10. The spectroscopic-based multi-wavelength liquid detection device of claim 1, wherein the light source is an LED.
CN202320837851.6U 2023-04-07 2023-04-07 Multi-wavelength liquid detection device based on light splitting technology Active CN219512087U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202320837851.6U CN219512087U (en) 2023-04-07 2023-04-07 Multi-wavelength liquid detection device based on light splitting technology

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202320837851.6U CN219512087U (en) 2023-04-07 2023-04-07 Multi-wavelength liquid detection device based on light splitting technology

Publications (1)

Publication Number Publication Date
CN219512087U true CN219512087U (en) 2023-08-11

Family

ID=87532303

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202320837851.6U Active CN219512087U (en) 2023-04-07 2023-04-07 Multi-wavelength liquid detection device based on light splitting technology

Country Status (1)

Country Link
CN (1) CN219512087U (en)

Similar Documents

Publication Publication Date Title
CN111239093A (en) Planar miniature multi-channel fluorescence detection optical system
CN205616889U (en) Gene sequencing optical devices
CN112414992A (en) Raman spectrum excitation enhancement module
CN102253014A (en) System and method for surface plasmon resonance sensing detection
CA2494478A1 (en) Fluorescence correlation spectroscopy instrument
US12099178B2 (en) Kinematic imaging system
CN1629618A (en) Multifunctional photoexcited surface plasma resonant imager
CN102253005A (en) Surface plasmon resonance sensing detection system and method
US11874453B2 (en) Optical system, method for calibrating optical system, and sequencing system
CN219512087U (en) Multi-wavelength liquid detection device based on light splitting technology
CN209759461U (en) optical system and sequencing system
CN113008786A (en) Blood cell analyzer
US11674877B2 (en) Apparatus and method for cyclic flow cytometry using particularized cell identification
CN101396765A (en) Laser machining device
US20080030721A1 (en) Optical apparatus and methods for chemical analysis
CN116699858B (en) Dodging device and gene sequencer
CN106399075B (en) A kind of detection system of the quantitative fluorescent PCR based on reflecting mirror
US20050285020A1 (en) Optical unit, optical sensor, multichannel optical sensing apparatus, and method for manufacturing optical unit
CN211627376U (en) Planar miniature multi-channel fluorescence detection optical system
CN213986200U (en) Raman spectrum excitation enhancement module
CN212674774U (en) Cell detection device based on multi-wavelength fluorescence technology
US20080007840A1 (en) Optical apparatus and methods for chemical analysis
CN2522855Y (en) Fluorescent detection scanning device for biological chip with filter piece
CN214097163U (en) PCR all-in-one machine and optical detection device thereof
CN221485213U (en) Detection device for multicolor fluorescence detection

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant