CN220907460U - Cell mixed solution sample adding device - Google Patents
Cell mixed solution sample adding device Download PDFInfo
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- CN220907460U CN220907460U CN202322144752.2U CN202322144752U CN220907460U CN 220907460 U CN220907460 U CN 220907460U CN 202322144752 U CN202322144752 U CN 202322144752U CN 220907460 U CN220907460 U CN 220907460U
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- conical
- cell mixed
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- 239000011259 mixed solution Substances 0.000 title description 29
- 239000011521 glass Substances 0.000 claims abstract description 24
- 239000000203 mixture Substances 0.000 claims description 38
- 239000007788 liquid Substances 0.000 abstract description 11
- 238000009826 distribution Methods 0.000 abstract description 7
- 238000007599 discharging Methods 0.000 abstract description 6
- 230000002311 subsequent effect Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 79
- 238000012360 testing method Methods 0.000 description 19
- 230000000694 effects Effects 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 6
- 238000013461 design Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 210000003855 cell nucleus Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000007480 spreading Effects 0.000 description 2
- 238000003892 spreading Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 210000004766 cell nucleus structure Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to the technical field of biology and discloses a cell mixed liquid sample feeder which comprises a sample feeding part and a sample discharging part, wherein the sample feeding part comprises a vertical end and a conical end which are fixedly connected, one end of the conical end far away from the vertical end is connected with the sample discharging part, an allocation block is further arranged in the conical end, the allocation block is conical, and a sample feeding gap exists between the side wall of the allocation block and the side wall of the conical end. The utility model has the advantages of reducing the flow speed of the cell mixed liquid during sample adding, enabling the cell mixed liquid to uniformly flow and sample adding, and enabling the distribution of cells in the multilayer liquid on the glass slide to be more uniform, thereby improving the subsequent effect of observing the cells.
Description
Technical Field
The utility model relates to the technical field of biology, in particular to a cell mixed liquid sample injector.
Background
Before the cell mixture is loaded onto a glass slide, in order to make the cells adhere to the glass slide more easily, and further facilitate the subsequent observation test, the viscosity of the cell surface lipid needs to be enhanced, the cell mixture is generally obtained by mixing a cell surface viscosity agent in the cell mixture, but when the cell mixture is loaded onto the glass slide later, the cell mixture is generally dropped onto the glass slide by an experienced operator directly through a dropper, a multilayer structure is gradually formed after the cell mixture drops are dropped onto the glass slide, if the flow rate of the mixture is uneven when the cell mixture drops are dropped onto the glass slide, the cell number distribution among different layers is uneven, and therefore, when the whole imaging is blurred when the cell mixture is observed by a microscope, the cell nucleus structure is not clearly seen, and finally the whole test progress is influenced, so that the technical requirements of the operator on the preparation of the sample are very high. Although the operation success rate of the experienced operators is high, a certain failure risk exists, and if the operation fails, the test is interrupted, so that the overall test progress is uncontrollable.
If the sample is applied by a person with little experience or just touching the sample, the sample is easily dropped onto the slide glass unevenly, which results in uneven distribution of cell numbers between different layers and failure of sample preparation. The cell mixture used in the test needs to spend a lot of time and effort to cultivate before the test is carried out, thus the cost of the whole test sample is higher, a plurality of slide samples are generally manufactured for comparison observation in a single test, if a plurality of samples are caused to be failed in manufacturing at the same time, the test cost is obviously increased, and the sustainable development of the test is not facilitated.
Therefore, aiming at the difficulties and pain points of the existing cell mixed solution in preparing samples, it is very necessary to design a tool for improving the uniformity of the cell mixed solution in sample adding, so that not only can the technical threshold of sample preparation operation be reduced, but also the success rate of sample preparation can be greatly improved, thereby reducing the loss of test samples and improving the efficiency of test development.
Disclosure of utility model
The utility model aims to provide a cell mixed solution sample adding device which is used for improving the uniformity of cell mixed solution sample adding.
In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a cell mixed solution application of sample ware, includes application of sample portion and play appearance portion, application of sample portion includes fixed connection's vertical end and conical end, the conical end keep away from the one end of vertical end with it is connected to go out appearance portion, the inside of conical end still is equipped with the piece of sharing, the piece of sharing is conical, the lateral wall of piece of sharing with there is the application of sample space between the lateral wall of conical end.
The principle and the advantages of the scheme are as follows: when the sample cell mixed solution is applied in practice, firstly, the prepared cell mixed solution to be detected and observed and the cell surface viscosity agent are mixed and then poured into the sample adding part, then the cell mixed solution drops on the split block, and the cell mixed solution respectively diffuses to the periphery from the split block and flows downwards, so that the cell mixed solution uniformly enters the sample outlet part, and the cell mixed solution uniformly drops on a glass slide to complete the preparation of a sample.
Compared with the prior art, the scheme can control the uniformity degree of the cell mixed solution during sample loading through the action of the split blocks, so that cells can not be accumulated in a large amount due to non-uniformity when forming multiple layers on a glass slide, and the cell nucleus is not clearly observed, thereby effectively improving the success rate of sample preparation, saving the test cost and also improving the feasibility of follow-up research operation. More importantly, the technical difficulty of the sample manufacturing process can be greatly reduced by the design of the scheme, so that the manufacturing of the sample is not dependent on the operation experience of operators, the sample manufacturing process is more suitable for the general public, the sample manufacturing efficiency can be effectively improved, and the efficiency of the whole test link is further improved.
Preferably, as a modification, the inner wall of the conical end is provided with a buffer structure for reducing the flow rate of the cell mixture. Through buffer structure, can reduce the velocity of flow of cell mixed solution, avoid its flow too fast to take place to pile up at the appearance portion of going out to influence the homogeneity degree of cell mixed solution.
Preferably, as a modification, the buffer structure is a stepped structure or a wavy structure.
Preferably, as a modification, the surface of the block includes a smooth surface and a concave-convex surface.
Preferably, as an improvement, a supporting structure for supporting the split blocks is arranged on the side wall of the split blocks.
Preferably, as an improvement, the supporting structure comprises three supporting rods which are uniformly distributed along the side surfaces of the allocation blocks, and the tail ends of the supporting rods are provided with anti-skid blocks. Through bearing structure, can make the amortization piece separate with the application of sample ware to both guaranteed the application of sample effect, do not influence other uses of application of sample ware again.
Preferably, as a modification, the material of the sample injector is glass or plastic.
Preferably, as a modification, the inclination angle of the conical end is 45-60 degrees. By limiting the inclination angle, the uniformity of the cell mixture during sample addition can be ensured, and the effect of controlling the flow rate of the cell mixture can be achieved.
Preferably, as a modification, one end of the spreading block away from the sample outlet portion is arc-shaped. By the arrangement, the cell mixture can be prevented from being accumulated on the split blocks when the split blocks act at the beginning, so that the change of the uniformity of cell distribution in the cell mixture is avoided.
Drawings
FIG. 1 is a schematic diagram of a cell mixture applicator according to an embodiment of the present utility model.
FIG. 2 is a schematic diagram of a block of an embodiment of a cell mixture applicator according to the present utility model.
FIG. 3 is a schematic view showing the inclination angle of a conical end of a cell mixture applicator according to an embodiment of the present utility model.
FIG. 4 is a schematic diagram of a second buffer structure of an embodiment of a cell mixture applicator according to the present utility model.
FIG. 5 is a schematic view of the upper end of a three-piece segment of an embodiment of a cell mixture applicator according to the present utility model.
Detailed Description
The following is a further detailed description of the embodiments:
The labels in the drawings of this specification include: sample adding part 1, vertical end 2, conical end 3, sample discharging part 4, sharing block 5, sample adding gap 6, supporting structure 7, supporting rod 8, non-slip block 9, inclination 10, buffer structure 11.
Embodiment one:
This embodiment is basically as shown in fig. 1 and fig. 2: the utility model provides a cell mixed solution sample injector, includes sample addition portion 1 and play appearance portion 4, and sample addition portion 1 includes integrated into one piece's vertical end 2 and conical end 3, and conical end 3's lower extreme is connected with play appearance portion 4, and both integrated into one piece, and play appearance portion 4 is hollow pipe, and sample addition portion 1 upper end is hollow pipe, and the lower extreme is hollow conical structure; a conical allocation block 5 is also arranged in the conical end 3, and a sample adding gap 6 is also arranged between the side wall of the allocation block 5 and the side wall of the conical end 3.
The side wall of the split block 5 is also provided with a supporting structure 7 for supporting the split block 5, the supporting structure comprises three supporting rods 8 which are uniformly distributed along the side face of the split block 5, the tail end of each supporting rod 8 is also provided with an anti-skid block 9, when the cell mixed solution is added, the split block can be directly and integrally placed at the conical end of the sample adding device to improve the uniformity degree of the cell mixed solution, and when the sample is not added, the split block can be integrally taken out, so that the sample adding device is convenient to use for completing the filling of other cell mixed solutions or other liquids, and the utilization rate of the sample adding device is effectively improved.
As shown in figure 3, the sample injector is made of glass or plastic, the inclined angle 10 of the conical end 3 is 45-60 degrees, and the surface of the allocation block 5 is a smooth surface or a concave-convex surface.
Specifically, in this embodiment, the sample injector is made of glass, and as shown in fig. 3, the inclination angle 10 of the conical end 3 is set to 60 degrees, and the surface of the spreading block 5 is a smooth surface.
The specific implementation process of this embodiment is as follows:
When the prepared cell mixture is to be loaded onto a glass slide for observation and research, the prepared cell mixture is firstly loaded into a container, then a sample loading device is placed right above the glass slide, the outlet of a sample discharging part 4 faces the glass slide, then the cell mixture is poured into the sample loading device from the container, the position of the container is controlled right above a conical end 3, the cell mixture is poured onto the conical end 3, then uniformly flows down along the side wall of the conical end 3 until the cell mixture enters the sample discharging part 4, and finally flows out of the outlet of the sample discharging part 4 and drops on the glass slide to finish the sample loading of the cell mixture.
Compared with the prior art that the cell mixed liquid directly utilizes the dropper to drop on the glass slide, the design of the sample adding device, particularly the conical end 3, can enable the cell mixed liquid to uniformly flow along the side wall of the conical end 3, and finally uniformly drop on the glass slide through the sample outlet part 4, and after the cell mixed liquid drops on the glass slide, the cell quantity distribution among different layers is more uniform when the cell mixed liquid drops are formed into a multilayer structure, a large number of cells are not accumulated at a certain position, so that the cell nuclei are not clearly seen when the microscope is utilized for observation, the test requirement is met, the success rate of test sample manufacturing is further ensured, the test loss is reduced, and the test cost is saved. Meanwhile, the sample injector in the scheme can replace the mode of using a dropper before, so that the technical difficulty of sample manufacture is reduced, the sample injector is not dependent on operation experience, beginners can master the sample injector more easily, and the culture of related talents is facilitated.
Embodiment two:
This embodiment is basically the same as embodiment one, except that:
As shown in fig. 4, the inner wall of the conical end 3 is also provided with a buffer structure 11 for reducing the flow rate of the cell mixture, and the buffer structure 11 is in a stepped structure or a wavy structure.
Specifically, the wave-shaped structure is selected in this embodiment.
Through this kind of setting, can increase the route that its flows when the cell mixture flows along the 3 lateral walls of cone end, and make the route more crooked to control its velocity of flow, avoid its quick flow to drip on the slide glass, lead to the cell inhomogeneous in the cell mixture's distribution, influence subsequent experimental effect.
Embodiment III:
This embodiment is basically the same as embodiment one, except that:
As shown in figure 5, the upper end of the split block 5 is arc-shaped, when the cell mixed solution is added, the cell mixed solution begins to drop on the split block from the right above the split block, then the cell mixed solution gradually flows downwards on the split block, and the sample addition of the cell mixed solution is completed.
Through this kind of setting, can make the cell mixed liquor when just beginning to contact the amortization piece, can not pile up on the amortization piece owing to the plane at amortization piece top, but through the guide effect of curved upper end, evenly flow downwards gradually to a plurality of directions, make the flow effect of cell mixed liquor more even, therefore cell distribution is also more even, avoid cell mixed liquid drop to form behind the multilayer structure to take place that cell piles up in a large number on the slide glass to it is unclear to lead to the nuclear observation, finally ensured experimental effect.
Embodiment four:
This embodiment is basically the same as embodiment one, except that:
The exit of the container of splendid attire cell mixed solution is provided with the valve, and when the cell mixed solution carried out the application of sample through the application of sample ware, the supply of utilizing valve intermittent type control cell mixed solution avoided cell mixed solution to continuously pour into the application of sample ware in, take place to pile up in the application of sample ware to influence the homogeneity degree when its application of sample, finally lead to cell mixed solution to appear the condition that the cell was piled up when forming multilayer structure on the slide glass, lead to the cell test failure.
The foregoing is merely an embodiment of the present utility model, and a specific structure and characteristics of common knowledge in the art, which are well known in the scheme, are not described herein, so that a person of ordinary skill in the art knows all the prior art in the application date or before the priority date, can know all the prior art in the field, and has the capability of applying the conventional experimental means before the date, and a person of ordinary skill in the art can complete and implement the present embodiment in combination with his own capability in the light of the present utility model, and some typical known structures or known methods should not be an obstacle for a person of ordinary skill in the art to implement the present utility model. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present utility model, and these should also be considered as the scope of the present utility model, which does not affect the effect of the implementation of the present utility model and the utility of the patent. The protection scope of the present utility model is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (9)
1. A cell mixed liquor sample injector, which is characterized in that: including application of sample portion and play appearance portion, application of sample portion includes fixed connection's vertical end and circular cone end, the circular cone end keep away from the one end of vertical end with it is connected to go out appearance portion, the inside of circular cone end still is equipped with the piece of sharing, the piece of sharing is conical, the lateral wall of piece of sharing with there is the application of sample space between the lateral wall of circular cone end.
2. The cell mixture applicator of claim 1, wherein: the inner wall of the conical end is provided with a buffer structure for reducing the flow velocity of the cell mixture.
3. A cell mixture applicator according to claim 2, wherein: the buffer structure is of a stepped structure or a wavy structure.
4. The cell mixture applicator of claim 1, wherein: the surface of the block includes a smooth surface and a concave-convex surface.
5. The cell mixture applicator of claim 1, wherein: and a supporting structure for supporting the split blocks is arranged on the side wall of the split blocks.
6. The cell mixture applicator of claim 5, wherein: the supporting structure comprises three supporting rods which are uniformly distributed along the side surfaces of the allocation blocks, and anti-skid blocks are arranged at the tail ends of the supporting rods.
7. The cell mixture applicator of claim 1, wherein: the sample injector is made of glass or plastic.
8. The cell mixture applicator of claim 1, wherein: the inclination angle of the conical end is 45-60 degrees.
9. The cell mixture applicator of claim 1, wherein: and one end of the apportioning block, which is far away from the sample outlet part, is arc-shaped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322144752.2U CN220907460U (en) | 2023-08-09 | 2023-08-09 | Cell mixed solution sample adding device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322144752.2U CN220907460U (en) | 2023-08-09 | 2023-08-09 | Cell mixed solution sample adding device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220907460U true CN220907460U (en) | 2024-05-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322144752.2U Active CN220907460U (en) | 2023-08-09 | 2023-08-09 | Cell mixed solution sample adding device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220907460U (en) |
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2023
- 2023-08-09 CN CN202322144752.2U patent/CN220907460U/en active Active
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