CN105861309A - Super-hydrophobic micro-pit array chip and preparation method and application thereof - Google Patents
Super-hydrophobic micro-pit array chip and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a super-hydrophobic micro-pit array chip and a preparation method and application thereof. The super-hydrophobic micro-pit array chip comprises a micro-pit array layer, and the surface, except micro-pits or the bottoms of the micro-pits, of the micro-pit array layer is the super-hydrophobic surface. The super-hydrophobic micro-pit array chip can be characterized in that the surface except the micro-pits is the super-hydrophobic surface, and the super-hydrophobic micro-pit array chip comprises the micro-pit array layer and a super-hydrophobic layer attached to the surface of the micro-pit array layer; the surface except the bottoms of the micro-pits is the super-hydrophobic surface, the micro-pit array layer comprises a base layer and a micropore array layer attached to the surface of the base, and micropore array layer is made of a super-hydrophobic material. The super-hydrophobic micro-pit array chip can achieve complete isolation among cell microarrays at a low cost in the cell microarray construction process, cross contamination of the cell arrays is avoided, good biocompatibility is guaranteed, and the super-hydrophobic micro-pit array chip has no obvious influence on normal growth of various cells and is suitable for high-throughput cell detection and analysis.
Description
Technical field
The present invention relates to a kind of super-hydrophobic micro-pit array chip and preparation method and application.
Background technology
Cell experiment is one of most basic research means of biological study.A lot of biological studies, such as high flux medicine
Thing screens, and stem cell combination type screening etc. relates to substantial amounts of cytologic experiment, and by substantial amounts of, process is the most extremely
Similar cell processing procedure realizes the optimization of some condition or result；Mostly traditional experiment operation is by manually many
Completing these cell experiments in orifice plate, waste time and energy, reagent consumption is very big, and research cost is higher.
Cell microarray (cell microarray) is the microcell array formed by chemistry, physical separation mode.
Such cell microarray is because flux is high, and the advantages such as reagent consumption is little, and cost is relatively low are more come in biological study
More come into one's own.Being chemically separated cell to be mostly to be modified by surface to realize, modifying by surface being done differentiation,
The cell dot matrix making cell can only grow at specific array position and to be formed.Physical separation mode can be by being formed
The form in micro-hole, intercepts cell and opens, how by injection mo(u)lding, or the mode of soft lithographic (soft lithography)
Preparation.Also there is the cell microarray platform simultaneously using Chemical Physics method to separate.
Cell microarray and realize the cell microarray platform of cell microarray high-flux medicaments sifting, cell transfecting,
The research such as stem cell differentiation has important function.
(1) high-flux medicaments sifting
Drug screening is the concept of a broad sense, contains a lot of aspects of drug screening.First, new in traditional sense
The screening of type drug molecule.Due to the progress of synthetic method, and natural organic molecules is constantly found, drug molecule
Storehouse is constantly expanding always, is administered target spot and also gets more and more, and the workload of drug screening increases sharp.Utilize high pass
It is the least that amount cell microarray carries out high-flux medicaments sifting compound molecule consumption, greatly reduces the one-tenth of drug screening
This.Second, drug screening also includes the individualized treatment option screening for individual.Individual for each, the state of an illness
Development and applicable medicine be not quite similar, in order to improve the effect of clinical treatment further, and timely and effectively
The containment state of an illness, it would be desirable to do preliminary drug screening for individual situation.This personalized therapy program is in cancer
Or the treatment of other difficult miscellaneous diseases has very important significance.Under normal circumstances, the patient that we can obtain is former
Being very limited amount of for cell, now, the meaning of high-flux cell array will highlight.Because if can use very
A small amount of cell forms high flux microarray, then we just can utilize a small amount of primary cell to carry out high-throughout medicine
Thing screens.So in this case, the effect of high-flux cell microarray platform almost can not be substituted.
(2) research cytogene and the function associating, exploring gene of phenotype is the important content of cytology research, leads to
Crossing and raise, lower or knock out certain gene, the phenotype of observation of cell, we are possibly able to determine some gene
Function.High-throughout cell microarray is that such research provides good platform.High flux reverses dye, slow virus
Transcribe research and all it turned out cell microarray great potential in gene functional research.
(3) stem cell is due to the unique totipotency of its own and proliferative, has very in terms of clinical regenerative medicine
Important potential value.But owing to the differentiation regulation of stem cell is the process of a multiple-factor impact, determining of stem cell
It is the key obstacle that stem cell moves towards clinical treatment to differentiation and proliferation.The influence factor of stem cell differentiation includes cellular matrix
Material, elasticity, solubility/insolubility factor, substrate pattern etc..In order to effectively decouple these influence factors, visit
The microenvironment of rope stem cell directional differentiation, high-throughout cell microarray is important platform.Stem cell, especially embryo
Tire stem cell, similar with primary cell, the amount usually obtained is little, and this microarray formats grinds actual
There is in studying carefully the biggest captivation.By regulation, combining all kinds of factor of influence, we can be at cell microarray platform
On realize high-throughout screening, be effectively facilitated stem cell differentiation research.
(4) high-flux cell microarray also has important meaning in terms of research cell secreta.It is at screening secretion pin
The cell of the monoclonal antibody of specific antigen is had obviously advantage, relative to traditional limiting dilution assay, high
Throughput cell microarray can significantly improve screening efficiency.
(5) in optical ultra-discrimination imaging field, random optical reconstruct microscope (STORM) be current resolution
One of high optical microscope.Biological research tool is of great significance by super-resolution imaging.A lot of sub-thin
Born of the same parents' structure is all at micron to nanoscale, and the existence of diffraction limit limits us and uses these lifes of observation by light microscope
Thing sample.The skelemin microfilament of such as cell is the most intensive, and under fluorescence microscope, its image is the fuzzyyest, it is impossible to
See details, and the resolution of ultramicroscope can reach about 1nm, most clearly present cytoskeleton
Details.But ultramicroscope is little to the sample labored, specificity does not has fluorescence microscope good yet.Super-resolution
Rate fluorescence microscopy is the most full-fledged, but operating procedure the most more redundant and complicated, and detection efficiency is the highest,
High flux super-resolution imaging is realized for accelerating the research and development in this field hence with high-flux cell microarray
Have very important significance.
In a word, high-flux cell Microarray Experiments platform is the research platform with important value, either grinds on basis
Study carefully and have very important value and significance the most in actual applications.
High-flux cell microarray technology platform includes that 384 or 1536 orifice plates (include corresponding automated machine at present
Platform), high flux micro-fluidic biological microreactor, PDMS (Polydimethylsiloxane)/PEG (Polyethylene
Glycol) micro-hole (and various other materials prepare micro-hole), and surface micro-patterning process after the cell that formed micro-
Array etc., they are primarily present following problem:
(1) use 384/1536 orifice plate to carry out physical separation, formation cell microarray detects for high throughput analysis,
It is typically necessary the supporting mechanical hand of employing or automation operating system, and the cost of this operating system is much more expensive,
Company is the most unable undertakes for common laboratory or small drug research and development.Which greatly limits on microwell plate
The use of high throughput analysis detection platform.
(2) in recent years, the cell detection platform developing into exploitation novel low-cost of microflow control technique have accumulated technology base
Plinth.Designed by complicated pump valve, can form cell microarray, and can by control the switch of pump valve will not
Same drug molecule specifically adds different cell dot matrix, thus realizes high-throughout pharmaceutical analysis.But it is this micro-
The structure of stream control platform is relative complex, is unsuitable for using in biological study field large-scale promotion.
(3) micro-hole prepared by PDMS micropore, PEG micropore or other various materials etc. are due to its preparation method letter
Single, the attention the most gradually causing people with low cost, become important Cytometric Analysis platform.But due to all
Micropore be submerged in same culture medium, the microenvironment of cellular array cannot independently control, such as, at high flux
In drug screening, water soluble molecules can occur to dissolve diffusion, thus is difficult to avoid cross-contamination, limits this kind of platform
Use.Therefore, Many researchers is devoted to solve this difficult problem, but result is unsatisfactory, because solving intersection
Other problem will be caused again while pollution problem.Such as, researcher is had to use the mode of oil sealing to completely cut off micro-hole, though
So can solve cross-contamination to a certain extent, but bring the most again the problems such as operation inconvenience.Also has research
The mode that person uses microtrabeculae to shut micro-hole solves cross-contamination, and this will cause the problem that aeration is not enough, hypoventilation again
Cell growth state will be made to be deteriorated, or make cell phenotype generation ANOMALOUS VARIATIONS.Also has research by micro-contact print
The mode of brush realizes dot matrixed modification in two-dimensional surface so that cell is merely able on the dot matrix of modified growth, thus
Form cell microarray, but this mode is not also due to growing environment is independently difficult to the intersection avoided between dot matrix
Pollute, and owing to micro-contact printing modifies less stable, along with cell incubation time increases, originally formed is thin
Can overlap between born of the same parents' array.
Additionally, the various micro element bio-compatible carrying out high-flux cell cultivation, detecting and analyze developed at present
Property is the most poor, it is impossible to ensure normal cell growth state, limits these micro elements in high-flux cell biology
Application in experiment.Such as, the doubling time of cell micro element cultivated often is far longer than cell in porous plate and increases
The speed grown；Cell on micro element is after cultivation after a while, and cell phenotype generation ANOMALOUS VARIATIONS, as cannot
Peculiar albumen expressing this cell etc..Therefore, most of micro element cannot be carried out environment highstrung former
For cell, the cultivation of stem cell and analysis, and exactly primary cell, stem cell high flux microarray are in personalized medicine,
The research and probe aspect of regenerative medicine has very important significance.
In terms of super-resolution imaging, STORM imaging is extremely complex to the processing procedure of sample, by high-flux cell
Microarray Experiments platform, can individually control the freestanding environment in each micro-hole, carries out large-scale STORM sample
Preparation, prepares compared to traditional STORM sample, and improved efficiency is notable, greatly improves super-resolution imaging letter
Breath density, reduces experimental cost.Meanwhile, the most still there is no to be effective to the platform of high flux high-resolution imaging,
The many high-resolution imagings carrying out multiple sample by the way of sample treatment one by one of experiment in the past, waste time and energy, efficiency
Lowly, the development of high-resolution imaging is largely limited.
Finally, the operation of current various high-flux cell microarray devices is the most complicated, such as, need the most micro-hole
Carrying out being loaded or change liquid operation, also the application on these cell microarray devices causes the biggest impact.
Summary of the invention
It is an object of the invention to provide a kind of super-hydrophobic micro-pit array chip and preparation method and application, this is super-hydrophobic super
Micro-pit array chip low cost, simple to operate, can avoid well in the case of cell normal growth state handing over ensureing
Fork pollutes, and can ensure good biocompatibility simultaneously, significantly reduces the cost that high-flux cell is analyzed, goes forward side by side
One step has promoted promoting the use of of high-flux cell detection technique, becomes in high-flux medicaments sifting, high flux super-resolution
The application such as picture all play an important role.
The one super-hydrophobic micro-pit array chip that the present invention provides, it includes micro-pit array layer, on described micro-pit array layer
Being provided with on the surface in micro-hole the surface in addition to bottom micro-hole or micro-hole is super hydrophobic surface.Super-hydrophobic table in the present invention
Face refers to that the contact angle with water (or aqueous solution) is more than 150 °, the roll angle surface less than 10 °.
The present invention super-hydrophobic micro-pit array chip, due to self special super-hydrophobic modification, can automatically form aqueous solution
Microlayer model array, thus ensure that microarray exists physical isolation, it is to avoid the cross-contamination between array.
In above-mentioned super-hydrophobic micro-pit array chip, shape, size, the degree of depth and the processing mode of described micro-pit array layer
Deng the most unrestricted.The experiment flux of the present invention super-hydrophobic micro-pit array chip is high, in common slide size
(76mm*26mm) more than 1000 droplet arrays can be built on chip；Reagent consumption is minimum, Mei Gewei
The volume in hole is about 50nL, greatly reduces the cost that high throughput testing is analyzed.
Described super-hydrophobic micro-pit array chip can be following (A) or (B):
(A) surface beyond described micro-hole is super hydrophobic surface, and described super-hydrophobic micro-pit array chip includes micro-hole battle array
Row layer and the super-hydrophobic layer being fitted on described micro-pit array layer surface.
Preferably, the thickness of described super-hydrophobic layer can be 10～150 microns (such as 100 microns).
The material of described super-hydrophobic layer, preparation method are unrestricted, and the such as present invention lists following example: by composition
Following super-hydrophobic pre-polymer solution: 24wt% propylacrylate methyl ester, 16wt% Ethylene glycol dimethacrylate,
60wt% 1-decanol (1-decanol) and 1wt% 2,2-dimethoxy-2-phenyl acetophenone inject two panels silicon after being sufficiently mixed
Between the slide of alkanisation, expose through uviol lamp, i.e. can get described super-hydrophobic layer；The method being fitted in basal layer includes
It is not limited to method of the present invention.
The material of described micro-pit array layer includes but not limited to polydimethylsiloxane (PDMS), poly-methyl methacrylate
Ester (PMMA, Polymethyl methacrylate), Merlon (PC, Polycarbonate), rustless steel, glass
Glass etc..
In order to meet the unique need of super-resolution imaging, described micro-pit array layer includes basal layer and is fitted in described substrate
The microwell array layer on surface, described basal layer can be super-resolution imaging special optics slide.This super-resolution imaging is special
100 times of object lens operating distances when can meet super-resolution imaging with super-hydrophobic micro-pit array chip, and be capable of automatically
Change high flux imaging.
(B) surface beyond bottom described micro-hole is super-hydrophobic, and described micro-pit array layer includes basal layer and laminating
At the microwell array layer of described substrate surface, described microwell array layer is made by super hydrophobic material.In the present invention
Super hydrophobic material refers to can be made into any material of super hydrophobic surface.
The material preparing described basal layer includes but not limited to polydimethylsiloxane (PDMS), poly-methyl methacrylate
Ester (PMMA, Polymethyl methacrylate), Merlon (PC, Polycarbonate), rustless steel, glass
Glass etc..
Invention further provides the manufacture method of a kind of above-mentioned super-hydrophobic micro-pit array chip, it includes described micro-
On the array layer of hole, surface in addition to bottom micro-hole or micro-hole is fabricated to the step of super hydrophobic surface.
Described method can be following method 1) or method 2):
1) surface in addition to micro-hole on described micro-pit array layer being fabricated to super hydrophobic surface, it comprises the steps
As follows:
In described micro-pit array layer, 1-a) it is provided with one layer of glue of surface adhesion in micro-hole；
1-b) the micro-pit array layer by adhesive gel is pressed against the surface of super-hydrophobic layer, takes micro-pit array layer off, i.e. after standing
The micro-pit array chip that surface is super hydrophobic surface beyond available above-mentioned micro-hole；
Specifically, in order to meet the unique need of super-resolution imaging, the method preparing described micro-pit array layer includes as follows
Step: at one layer of glue of surface adhesion of microwell array layer, be then fitted on the surface of basal layer, i.e. available described
Super-resolution imaging special micro-pit array chip.
The method can realize being minimal to the modification that becomes more meticulous of 200 microns, and method of modifying goes for variously-shaped
Patterning is modified, and this method of modifying hole micro-for substrate chip material is almost without selectivity, goes for
The super-hydrophobic modification of various materials micro-hole chip.
2) surface in addition to bottom micro-hole on described micro-pit array layer being fabricated to super hydrophobic surface, it includes as follows
Step:
2-a) in substrate, set up micro-pillar array；Described micro-pillar array concretely bears glue micro-pillar array, aobvious by exposure
Shadow.
2-b) take another substrate, with step 2-a) described in micro-pillar array align and be fixed together；Two substrates
Middle space irrigates super-hydrophobic prepolymer, cured rear separation, i.e. can get above-mentioned table in addition to bottom micro-hole
Face is the super-hydrophobic micro-pit array chip of super hydrophobic surface.
Above-mentioned super-hydrophobic micro-pit array chip is following 1)-8) any one of in application or have following 1 in preparation)
-8) application in the product of the function according to any one of, the most within the scope of the present invention.
1) high-flux cell microarray is built；
2) high-flux cell is cultivated；
3) high-flux cell analysis detection；
4) high-flux cell transfection；
5) high-flux medicaments sifting；
6) the combination type screening of high flux stem cell microenvironment；
7) high flux super-resolution imaging；
8) prepared by high flux STORM super-resolution imaging sample.
The present invention is on the basis of above-mentioned application, it is further provided a kind of utilize above-mentioned super-hydrophobic micro-pit array chip
The method cultivating cell, it comprises the steps a)-step c) at least one:
A) liquid addition steps, it comprise the steps: by solution above described super-hydrophobic micro-pit array chip unsettled dropwise
Drip, described liquid addition steps can be completed；
B) adding the step of cell, it is following b-1) or b-2):
B-1) after the micro-hole in described super-hydrophobic micro-pit array chip is full of by drop, by described super-hydrophobic micro-pit array
Chip submergence, is subsequently adding cell suspension, can will complete after cell natural subsidence described in add the step of cell；
B-2) in the super-hydrophobic micro-hole forming droplet array, add cell one by one, got final product place and stated the step adding cell
Suddenly；
C) changing the step of liquid, it comprises the steps:
C-1) described super-hydrophobic micro-pit array chip will add super-hydrophobic micro-pit array described in fresh culture fluid submergence
Chip, completes the exchange of new and old culture medium by standing；
C-2) take unnecessary culture fluid away, to re-forming droplet array, can complete described in change the step of liquid.
There is advantages that
(1) due to the super-hydrophobic modification of self uniqueness of described chip, super-hydrophobic micro-pit array can shape in micro-hole automatically
Becoming microlayer model array, this to facilitate by the way of mechanical arm hole-specifically adds cell relative in 384 traditional orifice plates
Many.Meanwhile, between the drop on this micro-pit array, it is entirely physical isolation, cross-contamination can be avoided well
Occur, be therefore highly suitable for the high flux screening of the soluble factor that tradition high flux platform is difficulty with, the highest
The screening of flux water soluble drug molecule.
(2) in the super-hydrophobic micro-pit array of the present invention, cultivation and the maintenance of cell microarray are very convenient, and the most prominent advantage exists
The simplest in changing liquid, it is only necessary to original droplet array to be immersed in fresh culture standing, waits that it fully expands
Interspersion can again form droplet array after changing and further cultivated and observe, and without conventional holes plate technique one by one
Carry out changing the operation of liquid.
(3) present invention super-hydrophobic micro-pit array biocompatibility is fabulous, it is entirely avoided high-flux cell microdevice before
Various problems: the cell microarray on the chip of super-hydrophobic micro-hole can be performed for more than six days drop formulas and cultivate, and before
Micro element on cell cultivate at most can only continue 96 hours, the overwhelming majority is only capable of maintaining less than 24 hours；Super
The growth rate that on the chip of hydrophobic micro-hole, cell microarray drop is cultivated is basically identical with common 24 orifice plates, and just keeps
Normal phenotype, the cell proliferation rate that micro element before is cultivated often is substantially less than cell proliferation speed in common orifice plate
Degree；Primary cell and the structure of stem cell microarray and cultivation is may be used on super-hydrophobic micro-pit array, and can be fine
Ground maintains the original character of these cells, and this is impossible to realize, at these on existing most of micro element
On micro element, cell may be unable to maintain that original phenotype so that the cell detection results on micro element loses credibility；
On super-hydrophobic micro-pit array, after the drop formula cultivation of 72h hour, survival rate is higher than 95%, with submergence for cell microarray
The result that formula is cultivated is close, and this shows that the present invention super-hydrophobic micro-pit array chip is cultivated cell growth and survived without obvious shadow
Ring.Additionally, difference in functionality molecule can be added by the way of point sample is loaded in different micro-holes.High-flux cell transfects
And stem cell microenvironment combination type analysis all proves that super-hydrophobic micro-hole is very suitable for various high throughput testing analysis.
In a word, the sharpest edges of the present invention super-hydrophobic micro-hole chip structure cell microarray are that it is building the micro-battle array of cell
Completely without the expensive robot device used in 384 traditional hole technology during row, therefore it can be with very
Low cost realizes the isolation completely between cell microarray, it is to avoid the generation of cross-contamination between cellular array, and with
It also assures that good biocompatibility simultaneously for this, (includes various cells than more sensitive primary cell and does thin
Born of the same parents) normal growth the most significantly affect, thus be extremely suitable for high-flux cell detection and analyze.
Accompanying drawing explanation
Fig. 1 is the structural representation of super-hydrophobic micro-pit array chip in embodiment 1.In Fig. 1, each labelling is as follows: 1 is micro-
Hole array layer, 2 super-hydrophobic layer.
Fig. 2 is the flow chart that in embodiment 1, micrografting prepares super-hydrophobic micro-pit array chip.
Fig. 3 is the photo of super-hydrophobic micro-pit array chip, the micro-hole SEM photograph prepared in embodiment 1 and connects
Feeler photo, wherein Fig. 3 (a) is the photo of super-hydrophobic micro-pit array chip, and Fig. 3 (b) is side in Fig. 3 (a)
Horizontal SEM photograph in frame region, Fig. 3 (c) is that the contact angle of super-hydrophobic layer in boxed area in Fig. 3 (b) shines
Sheet, Fig. 3 (d) is the longitudinal SEM photograph in Fig. 3 (b) in boxed area.
Fig. 4 is that the SEM of the super-hydrophobic micro-pit array chip of the variously-shaped and size prepared in embodiment 1 shines
Sheet.
Fig. 5 is the structural representation of super-hydrophobic micro-pit array chip in embodiment 2.In Fig. 5, each labelling is as follows: 1 base
Bottom, 2 Super-hydrophobic micropore array layers.
Fig. 6 is the flow chart of the synthetically prepared super-hydrophobic micro-pit array chip of embodiment 2 situ.
Fig. 7 is the photo of the super-hydrophobic micro-pit array chip prepared in embodiment 2.
Fig. 8 is the flow chart preparing the special super-hydrophobic micro-pit array chip of super-resolution imaging in embodiment 3.
Fig. 9 is formation droplet array spontaneous in the present invention super-hydrophobic micro-pit array chip.
Figure 10 is the liquid before and after the schematic diagram and process using and being coated with the most inswept super-hydrophobic chip liquid drop array surface of glue stick
The experimental result of drop volume variance and the SEM photograph of correspondence, wherein Figure 10 (a) is for using painting glue stick the most inswept super
The schematic diagram of hydrophobic chip liquid drop array surface, Figure 10 (b) be process before and after droplet size variance experimental result and
Corresponding SEM photograph.
Figure 11 carries out high-flux cell microarray cultivation and detection analysis for utilizing the present invention super-hydrophobic micro-pit array chip
Schematic diagram.
Figure 12 is to utilize the present invention super-hydrophobic micro-pit array chip to carry out used pressing down when high-flux cell microarray is cultivated
The device of drop processed volatilization, wherein, Figure 12 a is the structure of the device of control chip humidity when cultivating in incubator
Schematic diagram, Figure 12 b is the photo in kind of Figure 12 a.
Figure 13 is the experimental result utilizing the present invention super-hydrophobic micro-pit array chip to carry out drop formula cultivation, wherein Figure 13 a
For hamster kidney cell BHK-21, human umbilical vein epithelial cell HUVEC and the former leukaemia of the chronic marrow of people
After K562 cultivates the microphotograph behind 48 hours, 72 hours and 120 hours and Calcein AM/PI dyeing respectively
Microphotograph, Figure 13 (b) is hamster kidney cell BHK-21, human umbilical vein epithelial cell HUVEC enters respectively
Row drop formula cultivates the survival rate after cultivating with immersion, and Figure 13 (c) is hamster kidney cell BHK-21, people's umbilicus is quiet
Arteries and veins vascular endothelial HUVEC carries out immersion cultivation respectively, drop formula is cultivated and trains in common 24 orifice plates
The cell proliferation rate supported.
Figure 14 is the immunofluorescence dye after utilizing the super-hydrophobic micro-pit array of the present invention to cultivate HUVEC 72 hours
Color microphotograph and the result of one-tenth vascularization detection, wherein 14a is the result of CD 31 protein immunization fluorescence staining,
14b is the result of VE-cadherin protein immunization fluorescence staining, and 14c is into the result of vascularization detection.
Figure 15 is by adding different luminescent dye molecules and carry out height in the spray super-hydrophobic micro-pit array of sample present invention
Flux distribution and the experimental result of detection, wherein Figure 15 a is the photo after adding different luminescent dye molecule, Figure 15 b
For adding the photo of the luminescent dye molecule of variable concentrations, Figure 15 c is fluorescence intensity corresponding for Figure 15 b and fluorescent dye
The linear relationship chart of the concentration of molecule.
Detailed description of the invention
Experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, the most commercially obtain.
The composition of the hydrophobic polymer pre-polymer solution in following embodiment includes: 24wt% propylacrylate methyl ester
(butyl methacrylate, BMA), 16wt% Ethylene glycol dimethacrylate (ethylene dimethacrylate,
EDMA), 60wt% 1-decanol (1-decanol) and 1wt% 2,2-dimethoxy-2-phenyl acetophenone are (relative to monomer
For quality summation) (2,2-dimethoxy-2-phenylacetophenone, DMPAP) (be all purchased from
Sigma-Aldrich).Preparation method be the raw material of said ratio is sufficiently mixed 1h on Syrup-homogenizing instrument after standby.
Slide in following embodiment is the most all carried out and alkylation, and cleaning step is: use 98% is dense
Sulphuric acid and 30% hydrogen peroxide mixture (volume ratio=7:3) clean slide 30min, then clean slide three with deionized water
Secondary, nitrogen thoroughly dries up standby.The step that alkylation is modified is: use 20% (volume ratio) 3-(trimethoxy is silica-based)
Propylacrylate methyl ester (3-(trimethoxysilyl) propyl methacrylate) ethanol solution is to the glass after above-mentioned cleaning
Sheet carries out submergence can complete silylation modification, cleans three times with acetone after 1h, and nitrogen dries up standby.
Embodiment 1, micrografting prepare super-hydrophobic micro-pit array chip
The structural representation of super-hydrophobic micro-pit array chip is as it is shown in figure 1, it includes micro-pit array layer 1 and is fitted in micro-
Super-hydrophobic layer 2 on array layer surface, hole, wherein, the thickness of super-hydrophobic layer 2 is 100 microns.
As it is shown in figure 1, utilize micrografting technology to prepare super-hydrophobic micro-pit array chip, specifically comprise the following steps that
A () spin coating: as a example by Dow Corning 3140, the rotating speed spin coating 30s with 7000rpm can be at PMMA
The thinnest one layer viscose is obtained on layer.
B prepared by () PDMS micro-hole chip: use the silicon chip SU-8 mould processed (by Bo Ao Biochip company
Customization processing), after wrapping with tinfoil, the container as reverse mould is standby.Preparation PDMS pre-polymer solution, monomer
Being 10:1 (purchased from Dow Corning company, catalog number (Cat.No.) SylgardR 184) with the volume ratio of catalyst, Glass rod fills
Bubble during evacuation 30min removes solution in vacuum drying oven is put into after point stirring and evenly mixing.Again pre-polymer solution is poured on
On SU-8 mould, put into 80 DEG C of baking oven polymerization more than 2h and take off standby again from mould.
C () takes off i.e. after micro-for above-mentioned PDMS hole chip is pressed lightly on the thin layer viscose obtained in step (a) again
One layer of thin glue can be adhered at the upper surface of micro-hole chip.
D () takes two panels silanised glass slides, the double-sided tape conduct after the two ends of a piece of slide stick 150 microns
Spacer, then another sheet slide is adhered on above-mentioned slide, the super-hydrophobic pre-polymer solution configured slowly is injected two panels
In the gap of slide, then it is placed under uviol lamp (302nm, CL-1000Crosslinker, UVP) exposure 15min.
E () pries open two panels slide with blade can obtain the super-hydrophobic layer of one layer of 100 μm on lower floor's slide.Should
Super-hydrophobic layer submergence is cleaned three times in ethanol, standby after drying up.
(f) transfer super-hydrophobic layer: the micro-hole chip having adhered to thin glue step (c) obtained is pressed against step (e)
In the super-hydrophobic layer obtained, taking micro-hole chip after standing more than 4h off can surpass the upper strata of micro-hole chip transfer one layer
Hydrophobic layer, obtains super-hydrophobic micro-hole battle array chip.
The photo of super-hydrophobic micro-pit array chip, micro-hole SEM photograph and the contact angle photo that the present embodiment prepares,
As shown in Figure 3.
In the present embodiment, super-hydrophobic micro-pit array-processing method based on micrografting technology can realize being minimal to 200 microns
The modification that becomes more meticulous, method of modifying goes for variously-shaped patterning and modifies (as shown in Figure 4), and this
Method of modifying hole micro-for substrate chip material, almost without selectivity, goes for the super of various material micro-hole chip
Hydrophobic modification.
Embodiment 2, fabricated in situ prepare super-hydrophobic micro-pit array chip
The structural representation (side) of super-hydrophobic micro-pit array chip is as it is shown in figure 5, it includes basal layer 1 and laminating
Super-hydrophobic micropore array layer 2 (microwell array layer 2 is made by super hydrophobic material) at substrate surface.
As shown in Figure 6, utilize situ synthesis techniques to prepare super-hydrophobic micro-pit array chip, specifically comprise the following steps that
A prepared by () negative glue micro-pillar array: on the slide after this silanization, even 100 μ m-thick bear glue, and exposure imaging obtains
It is 100 μm to height, a diameter of 500 μm, the micro-pillar array of distance of center circle 1000 μm.
(b) super-hydrophobic layer in-situ polymerization: use after the slide of the slide and another silanization with micro-pit array is alignd
Clamp, slowly injects the super-hydrophobic pre-polymer solution configured in the micro-pillar array space between two slides, then
It is placed under uviol lamp (302nm, CL-1000 Crosslinker, UVP) exposure 15min, finally pries open two panels with blade
Slide can be without obtaining the 100 deep micro-pit arrays of μm on the slide of micro-pillar array, finally three times can be standby with ethanol purge
With, obtain super-hydrophobic micro-pit array chip.
The photo of the super-hydrophobic micro-pit array chip that the present embodiment prepares is as shown in Figure 7.
Embodiment 3, the special super-hydrophobic micro-pit array chip of super-resolution imaging
The present embodiment is unique need based on super-resolution imaging, and array preparation method at the bottom of micro-pit foundation has been carried out corresponding tune
Whole, the structural representation of super-hydrophobic micro-pit array chip is identical with Fig. 1, including micro-pit array layer 1 be fitted in micro-hole
Super-hydrophobic layer 2 on array layer surface, is only that micro-pit array layer 1 is by basal layer (super-resolution imaging special optics glass
Sheet) and it is fitted in this suprabasil microwell array layer composition.
Making programme as shown in Figure 8, specifically comprises the following steps that
A () spin coating: as a example by Dow Corning 3140, the rotating speed spin coating 30s with 7000rpm can be at PMMA
The thinnest one layer viscose is obtained on layer.
Prepared by (b) photoresist dry film microarray: with 100 microns of thick photoresist dry films through front baking, exposure, after bake,
It is standby that the processes such as development obtain microwell array film.
C photoresist microwell array film is pressed lightly on that take off after this thin layer viscose can be in micropore battle array again by () and (d)
One layer of thin glue is adhered on row film.
E () attaches microwell array film: the microwell array film having adhered to thin glue is pressed against the special optics of super-resolution imaging
On slide, this film can be attached on optics slide after standing more than 4h.
F () and (g) prepares super-hydrophobic layer: preparation process is with the step (c) in embodiment 1 and (d).
H () spin coating again: as a example by Dow Corning 3140, can be with the rotating speed spin coating 30s of 7000rpm
The thinnest one layer viscose is obtained on PMMA layer.The photoresist microwell array film attached is pressed lightly on and sticks at this thin layer
Take off again after glue and can adhere to one layer of thin glue on microwell array film.
I the preparation of () micro-pit array chip: be pressed against in super-hydrophobic layer by the microwell array having adhered to thin glue, stands 4h
Below take micro-hole chip afterwards off and can shift one layer of super-hydrophobic layer on the upper strata of microwell array, obtain super-hydrophobic micro-pit array
Chip.
The effect of embodiment 4, the present invention super-hydrophobic micro-pit array chip and purposes
One, the homogeneity of droplet array and drop is spontaneously formed
Owing to the super-hydrophobic layer in the present invention super-hydrophobic micro-pit array chip has strong repulsive interaction to aqueous solution, because of
This can spontaneously form droplet array.Generation type is as follows: first will utilize weight by the drop that dropwise drips that dangles
Power impulsive force makes aqueous solution enter micropore can form droplet array (as shown in Figure 9) in super-hydrophobic micro-pit array；
Once formed after drop can by whole chip submergence, then take unnecessary aqueous solution away again can be spontaneous in micro-pit array
Forming droplet array, this process can be carried out with iterative cycles.Therefore can be easily on this super-hydrophobic chip
Form high-flux cell microarray, add cell without hole the most micro-by the way of mechanical arm point sample and form cell
Array, ensures complete physical isolation between micro-pit array simultaneously, substantially avoid cross-contamination.
Use and be coated with the most inswept super-hydrophobic chip liquid drop array surface of glue stick, the homogeneity of droplet array can be significantly improved,
As shown in Figure 10, the variance of droplet size is reduced to 1% from 11%.It is fixed that this illustrates that super-hydrophobic micro-pit array can be carried out
Amount detection is analyzed.Additionally, the droplet size of the super-hydrophobic micro-pit array of the present invention is at about 50nL, many relative to tradition
In orifice plate, the volume of micro updating significantly reduces the consumption of reagent, greatly reduces the one-tenth that high-flux cell detection is analyzed
This.
Two, the present invention super-hydrophobic micro-pit array chip application in the formation, cultivation of high-flux cell microarray
(1) experiment material
DMEM basic (1 ×) culture medium (article No.: C11995500B7), FBS (Fetal Bovine Serum, tire cattle
Serum, article No.: 10099-141), dual anti-(penicillin penicillin and streptomycin streptomycin) (above-mentioned all purchases
From Thermal Fisher).PBS (phosphate buffer saline, phosphate buffer) pH 7.4basic (1 ×) (purchases
From Gibco, Life technologies, article No.: C10010500BT), (calcein, purchased from Thermal for Calcein AM
Fisher, article No. C3100MP), PI (bromination the third pyridine, purchased from Sigma Aldrich, article No. 25535), formalin is molten
Liquid (purchased from Sigma Aldrich, article No. 252549), Triton-X 100 is (purchased from Sigma Aldrich, article No.
X100-500ML), BSA (bovine serum albumin, Bovine Serum Albumin, purchased from AMRESCO, article No.:
0332-100g), mouse-anti people's CD31 monoclonal antibody (purchased from Sigma, article No. SAB4700463-100 μ g), mouse-anti people
VE-cadherin monoclonal antibody (purchased from Santa Cruz Biotechnology, article No.: SC-9989), the anti-(Alexa of mountain sheep anti mouse two
Fluor 488 goat anti-mouse antibody, purchased from Life Technologies, article No.: A10667), Tween 20,
DAPI (mentioned reagent is purchased from Sigma Aldrich), Matrigel (purchased from BD company, article No. 356231), pancreatin
(HyClone Trypsin 0.25% (1 ×) solution, purchased from GE Healthcare Life Sciences, article No.:
SH30042.01), hamster kidney cell BHK-21 (purchased from English luxuriant industry bio tech ltd), human umbilical vein
Epithelial cell HUVEC is (purchased from bio tech ltd of Nanjing section one hundred, brand ATCC, catalog number
And the former K562 Leukaemia of the chronic marrow of people is (purchased from bio tech ltd of Nanjing section one hundred, brand CBP60340)
ATCC, catalog number CBP60529).
Fluorescence inverted microscope (IX83, Olympus), vacuum pump (Haimen City its woods Bel instrument manufacturing company limited,
GL-802B type), incubator (HERAcell 150i, purchased from Thermal Scientific)
(2) experimental procedure:
A, drop formula of the present invention are cultivated
As shown in figure 11, the present invention super-hydrophobic micro-pit array chip is utilized to realize cultivation and the inspection of high-flux cell microarray
Cls analysis, the operation of whole cell microarray mainly includes super-hydrophobic micro-hole liquid feeding, adds cell, forms drop, drop formula
Cultivate, change multiple steps such as liquid, specific as follows:
1) super-hydrophobic micro-hole liquid feeding: owing to the super-hydrophobic layer of grafting has strong repulsive interaction to aqueous solution, the most carefully
Born of the same parents' culture fluid can not add according to the mode of conventional liquid feeding, and the mode that the present invention uses hanging drop to pound adds in micro-hole
Liquid, (HyClone DMEM high sugar liquors culture medium, is purchased from i.e. to draw DMEM solution with the pipette of 10mL
Sai Mo flies your biochemistry Products Co., Ltd of generation) after, above chip, unsettled 20cm dropwise drips, and drop passes through
Action of gravity overcomes the repulsion of super-hydrophobic layer to enter micro-hole.
2) cell is added: have two ways to add cell in micro-hole at present.First kind of way be when in all micro-holes all by
After drop is full of, by whole chip submergence, be subsequently adding cell suspension, shake even after make cell natural subsidence, can be by
Cell adds in each micro-hole.The second way is in super-hydrophobic micro-hole after formation droplet array, by hand-held liquid feeding
Cell number needed for ground, rifle the most micro-hole adds cell, such Adding Way is considerably less, can be expected to obtain
The high throughput testing of the precious cell sample that quantity is few plays an important role.
3) immersion cultivate: by above-mentioned cell solution dot matrix again with culture medium (DMEM high glucose medium, 10%
FBS, 100U/mL penicillin, and 100 μ g/mL streptomycins) submergence, hole-specifically take pictures and determine initial cell number,
Then start drop formula to cultivate.
4) droplet formation: the unnecessary culture medium of submergence chip is taken away completely and just can spontaneously form droplet array.
5) drop formula is cultivated: in order to suppress evaporation when high-flux cell microarray is cultivated in incubator, uses suit
Super-hydrophobic chip (such as Figure 12 a, shown in 12b) placed by culture dish, and adds aquesterilisa in the culture dish of outer layer, it is ensured that
The air humidity of droplet array microenvironment reaches saturated.
6) change liquid: after 12h drop formula is cultivated, add the super-hydrophobic chip of fresh culture submergence, stand 10min,
Make fresh culture can fully diffuse in super-hydrophobic micro-hole, complete the exchange of new and old culture medium.Liquid is changed in submergence
While hole-specifically cell is taken pictures, add up cell number, with cell relative to the Ln value of proliferation times as vertical coordinate,
The growth curve of cell is drawn with generation time for abscissa.Finally more unnecessary culture medium is taken away, can shape again
Become droplet array, in order to carry out further drop and cultivate propagation.This process can move in circles and carry out, therefore originally
Invent super-hydrophobic micro-pit array chip and can carry out the cultivation of high-flux cell microarray long drop formula.
7) cell survival rate analysis: Cell sap drip is cultivated after terminating, and adds 5mL PBS solution submergence cell drop
Array, is simultaneously introduced 50 μ L Calcein AM (100 ×) solution and 5 μ L PI solution (1000 ×), 37 after mixing
30min is hatched under the conditions of DEG C.Hole-specifically take pictures under microscope, observe the survival rate after statistics Cell sap drip is cultivated.
Calcein AM can be hydrolyzed to calcein under the effect of living cells endoenzyme, sends green fluorescence, therefore green fluorescence
Passage show for living cells；PI can penetrate the cell membrane of dead cell or apoptotic cell, with endonuclear DNA
Double-strand combines and sends red fluorescence, so red fluorescence channel display dead cell or apoptotic cell.
8) immunofluorescence dyeing experiment: HUVEC cell 72h drop formula is cultivated after terminating, and needs to carry out immunofluorescence
Coloration experiment verifies whether they can two kinds of albumen of specific expressed CD31 and VE Cadherin.First train at drop
Support and fix HUVEC cell on super-hydrophobic chip 10 minutes with formalin solution after terminating, then formalin is inhaled
Go, wash three times (formalin solution is poisonous, should be collected in waste liquid bottle) with PBS.With confining liquid (PBS+0.05%
Triton-X 100+2% BSA) close 2 hours.Suck confining liquid, with confining liquid, anti-human CD31 antibody is diluted
100 times, adding 200uL on micropore, 4 degree of overnight incubation (around add some liquid, seal up with sealed membrane, prevent
Evaporation becomes dry).Wash three times with cleaning buffer solution (PBS+0.05% Tween 20), each 5 minutes, slowly shake.
Again with confining liquid by two anti-dilution 100 times, add 200uL on micropore, incubated at room 2 hours, note lucifuge.Clearly
Wash buffer is washed three times, each 5 minutes.Add DAPI dyeing liquor 200uL in tissue, hatch 10 minutes, PBS
Wash three times.Note lucifuge.Fluorescence microscope.The dyeing course of VE Cadherin is similar to.
9) vascularization detection is become: 72h drop formula is cultivated after terminating, and with PBS solution submergence droplet array, fully expands
Scattered exchange cleans up the culture medium in micropore, is then taken away by PBS, adds trypsin solution and covers microwell array,
After cell dissociation 1min, trypsin solution is taken away, add culture medium by chip submergence, blow the most piecewise with liquid-transfering gun
Beat and cell is blown out, then cell solution is transferred in centrifuge tube, after being centrifuged, take the unnecessary supernatant away so that
Whole cell substantially concentration is 1-3 × 10^5 cell.Meanwhile, get out the culture dish that Matrigel processes, will
The HUVEC cell that digestion obtains is added in the culture dish that Matrigel processes, and continues to cultivate and observes, verifies HUVEC
Cell the most still has into the potentiality of blood vessel.
Chronic to hamster kidney cell BHK-21, human umbilical vein epithelial cell HUVEC and people according to above-mentioned steps
The former K562 Leukaemia of marrow carries out drop formula cultivation, and condition of culture is as follows: 37 DEG C, 5% CO2Incubator.
B, comparison one: immersion is cultivated
As comparison, above-mentioned cell being carried out immersion cultivation, step is as follows simultaneously:
1) super-hydrophobic micro-hole liquid feeding: owing to the super-hydrophobic layer of grafting has strong repulsive interaction to aqueous solution, therefore
Cell culture fluid can not add according to the mode of conventional liquid feeding, and we use mode that hanging drop pounds in micro-hole here
Liquid feeding, (HyClone DMEM high sugar liquors culture medium, purchases i.e. to draw DMEM solution with the pipette of 10mL
From match Mo Feishier biochemistry Products Co., Ltd) after, above chip, unsettled 20cm dropwise drips, and drop leads to
Crossing action of gravity overcomes the repulsion of super-hydrophobic layer to enter micro-hole.
2) cell is added: the first adds cellular modalities is direct submergence super-hydrophobic micro-hole chip, then adds in chip culture dish
Enter 46 × 10^4 cell solution.After mixing, standing sedimentation 10min takes unwanted cell solution away, can be formed thin
Cell lysis liquid dot matrix.It is in super-hydrophobic micro-hole after formation droplet array that the second adds cellular modalities, by hand-held liquid feeding rifle
Cell number needed for ground, the most micro-hole adds cell, such Adding Way is considerably less, can be expected to obtain number
Measure in the high throughput testing of few precious cell sample and play an important role.
3) immersion is cultivated: above-mentioned cell solution dot matrix is used culture medium submergence again, hole-specifically takes pictures and determine initiator cell
Number, then starts immersion and cultivates, condition of culture 37 DEG C, 5% CO2Incubator.
4) liquid is changed: after 12h immersion is cultivated, take original culture medium away, add fresh culture submergence
Super-hydrophobic chip, the most hole-specifically takes pictures to cell, adds up cell number, with cell relative to the Ln of proliferation times
Value is vertical coordinate, draws the growth curve of cell with generation time for abscissa.Proceed immersion to cultivate, every
12h changes a not good liquor.
5) cell survival rate analysis: Cell sap drip is cultivated after terminating, and adds 5mL PBS solution submergence cell drop
Array, is simultaneously introduced 50 μ L Calcein AM (100 ×) solution and 5 μ L PI solution (1000 ×), 37 after mixing
30min is hatched under the conditions of DEG C.Hole-specifically take pictures under microscope, observe the survival rate after statistics Cell sap drip is cultivated.
Calcein AM can be hydrolyzed to calcein under the effect of living cells endoenzyme, sends green fluorescence, therefore green fluorescence
Passage show for living cells；PI can penetrate the cell membrane of dead cell or apoptotic cell, with endonuclear DNA
Double-strand combines and sends red fluorescence, so red fluorescence channel display dead cell or apoptotic cell.
C, comparison two: common 24 orifice plates are cultivated
As comparison, being cultivated by above-mentioned cell in common 24 orifice plates, step is as follows simultaneously:
1) cell is added: directly add in cell solution, cell initial density and Super-hydrophobic micropore array in 24 orifice plates
Cell density is suitable.
2) cultivate: 24 orifice plates are placed in 37 DEG C, 5% CO2Incubator is cultivated 12h.
3) counting: after 12h cultivates, takes 6 hole statistics cell densities, with cell relative to proliferation times simultaneously
Ln value be vertical coordinate, with generation time be abscissa draw cell growth curve.
4) liquid is changed: except other cell types of K562 extracellular are both needed to carry out change liquid operation, in addition to counting hole, by it
The culture fluid in remaining each hole is taken away, adds the fresh culture of isodose, continues at 37 DEG C, 5% CO2Incubator is trained
Support 12h, take 6 holes statistics cell densities the most again, with cell relative to the Ln value of proliferation times as vertical coordinate, with
Generation time is the growth curve that abscissa draws cell.
(3) experimental result:
Cultivation results is as shown in figure 13.The biocompatibility of the super-hydrophobic micro-pit array of the present invention is the best, can surpass
The high-flux cell microarray of spending 6 days is cultivated, and can carry out the high flux suspension cell that most of platform is difficulty with micro-
Array is cultivated.The super-hydrophobic micro-pit array of the present invention can carry out primary cell, the high flux microarray formula of stem cell
Long-time cultivation (shown in Figure 13 a)；After the drop formulas of tens of hours are cultivated, cell also is able to keep higher depositing
Motility rate, the only cell less than 5% can occur apoptosis or death, result quite (Figure 13 b institute cultivated with immersion
Show)；The cell proliferation rate that cell microarray on the chip of super-hydrophobic micro-hole is cultivated and the propagation speed in common 24 orifice plates
Spend basically identical (shown in Figure 13 c).
Primary cell microarray on the super-hydrophobic micro-pit array of the present invention, as a example by HUVEC, trains through 72h drop formula
It also is able to the most specific expressed CD31, VE-cadherin both albumen after Yanging, also maintains into blood vessel simultaneously
The function (as shown in figure 14) changed.
Three, the super-hydrophobic micro-array chip of the present invention is in the application analyzed in detection of high-flux cell microarray
The super-hydrophobic micro-pit array of the present invention, owing to achieving the physical isolation of droplet array, therefore can pass through easily
The luminescent dye molecule (rhodamine B and Fluorescein isothiocyanate) of two kinds of colors red, green is added every row by spray sample technology
Add in different micro-holes (as shown in fig. 15 a), thus realize the high throughput testing to cell microarray and analysis.Figure
15a is shown in micro-hole that the dyestuff of different colours can be added different lines by spray sample, no cross contamination between micro-hole
Occur.It is also possible to add gfp molecule Fluorescein isothiocyanate with realizing quantification, it is achieved right
The Concentraton gradient of compound molecule adds (shown in Figure 15 b and Figure 15 c).
Claims (9)
1. a super-hydrophobic micro-pit array chip, it includes micro-pit array layer, it is characterised in that: described micro-pit array
It is super hydrophobic surface that layer is provided with on the surface in micro-hole the surface in addition to bottom micro-hole or micro-hole.
Super-hydrophobic micro-pit array chip the most according to claim 1, it is characterised in that: beyond described micro-hole
Surface is super hydrophobic surface, and described super-hydrophobic micro-pit array chip includes micro-pit array layer and is fitted in described micro-pit array
Super-hydrophobic layer on layer surface.
Super-hydrophobic micro-pit array chip the most according to claim 2, it is characterised in that: described super-hydrophobic layer
Thickness is 10～200 μm.
4. according to the super-hydrophobic micro-pit array chip described in Claims 2 or 3, it is characterised in that: described micro-hole battle array
Row layer includes basal layer and is fitted in the microwell array layer of described substrate surface, and described basal layer is that super-resolution imaging is special
Use optics slide.
Super-hydrophobic micro-pit array chip the most according to claim 1, it is characterised in that: bottom described micro-hole with
Outer surface is super-hydrophobic, and described micro-pit array layer includes basal layer and is fitted in the microwell array of described substrate surface
Layer, described microwell array layer is made by super hydrophobic material.
6. the manufacture method of the super-hydrophobic micro-pit array chip according to any one of claim 1-5, it is characterised in that:
It includes the step that surface in addition to bottom micro-hole or micro-hole on described micro-pit array layer is fabricated to super hydrophobic surface.
Manufacture method the most according to claim 6, it is characterised in that: described method is following 1) or 2):
1) surface in addition to micro-hole on described micro-pit array layer being fabricated to super hydrophobic surface, step is as follows:
In described micro-pit array layer, 1-a) it is provided with one layer of glue of surface adhesion in micro-hole；1-b) by micro-hole battle array of adhesive gel
Row layer is pressed against the surface of super-hydrophobic layer, takes micro-pit array layer off after standing, and i.e. can get in claim 2-4 and appoints
One described micro-pit array chip；
2) surface in addition to bottom micro-hole on described micro-pit array layer being fabricated to super hydrophobic surface, step is as follows:
2-a) in substrate, set up micro-pillar array；2-b) take another substrate, with step 2-a) described in micro-pillar array pair
Neat and be fixed together；Space in the middle of two substrates irrigates super-hydrophobic prepolymer, cured rear separation, to obtain final product
Super-hydrophobic micro-pit array chip described in claim 5.
8. the super-hydrophobic micro-pit array chip according to any one of claim 1-5 is following 1)-8) any one of or
Preparation have following 1)-8) and any one of function product in application:
1) high-flux cell microarray is built；
2) high-flux cell is cultivated；
3) high-flux cell analysis detection；
4) high-flux cell transfection；
5) high-flux medicaments sifting；
6) the combination type screening of high flux stem cell microenvironment；
7) high flux super-resolution imaging；
8) prepared by high flux STORM super-resolution imaging sample.
9. the super-hydrophobic micro-pit array chip utilized according to any one of claim 1-5 carries out the side of cell cultivation
Method, it includes following a)-c) at least one:
A) liquid addition steps, it comprise the steps: by solution above described super-hydrophobic micro-pit array chip unsettled dropwise
Drip, described liquid addition steps can be completed；
B) adding the step of cell, it is following b-1) or b-2):
B-1) after the micro-hole in described super-hydrophobic micro-pit array chip is full of by drop, by described super-hydrophobic micro-pit array
Chip submergence, is subsequently adding cell suspension, can will complete after cell natural subsidence described in add the step of cell；
B-2) in the super-hydrophobic micro-hole forming droplet array, add cell one by one, got final product place and stated the step adding cell
Suddenly；
C) changing the step of liquid, it comprises the steps:
C-1) described super-hydrophobic micro-pit array chip will add super-hydrophobic micro-pit array described in fresh culture fluid submergence
Chip, completes the exchange of new and old culture medium by standing；
C-2) take unnecessary culture fluid away to re-forming droplet array, can complete described in change the step of liquid.
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| PCT/CN2017/078946 WO2017177839A1 (en) | 2016-04-14 | 2017-03-31 | Super-hydrophobic micro-pit array chip, preparation method therefor and applications thereof |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| WO2017177839A1 (en) * | 2016-04-14 | 2017-10-19 | 清华大学 | Super-hydrophobic micro-pit array chip, preparation method therefor and applications thereof |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1434296A (en) * | 2003-03-04 | 2003-08-06 | 成都夸常科技有限公司 | Biological chip with minimized reactor isolation structure height and its preparation method |
| CN1858593A (en) * | 2006-03-23 | 2006-11-08 | 厦门大学 | Hydrophilic and phydrophobic mode sheet base special for biological chip |
| CN101481081A (en) * | 2009-01-21 | 2009-07-15 | 重庆大学 | Preparation of composite structure super-hydrophobic film |
| CN102439717A (en) * | 2009-03-24 | 2012-05-02 | 芝加哥大学 | Slip chip device and method |
| US20120302467A1 (en) * | 2010-11-29 | 2012-11-29 | Karlsruher Institut Fur Technologie | Patterned substrates for cell applications |
| CN103335984A (en) * | 2013-06-09 | 2013-10-02 | 清华大学 | Microarray chip without solid wall based on LSPR (Localized Surface Plasmon Resonance) and application thereof |
| CN103753984A (en) * | 2014-01-25 | 2014-04-30 | 深圳清华大学研究院 | Stamp, manufacturing method of stamp and manufacturing method of drop array |
| CN104762207A (en) * | 2015-04-24 | 2015-07-08 | 何向锋 | Special culture membrane for single cell cloning separation and single cell cloning separation method |
| CN104774763A (en) * | 2015-04-24 | 2015-07-15 | 何向锋 | Microchip type single cell cloning separation culture plate and single cell cloning separation method |
| CN105463075A (en) * | 2015-11-19 | 2016-04-06 | 北京科技大学 | Method for detecting miRNA based on super-hydrophilic micro-well sensing interface |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010107497A2 (en) * | 2009-03-18 | 2010-09-23 | The Regents Of The University Of California | Honeycomb shrink wells for stem cell culture |
| KR101188011B1 (en) * | 2011-06-03 | 2012-10-08 | 삼성전기주식회사 | Bio chip |
| US20130029422A1 (en) * | 2011-07-26 | 2013-01-31 | Vasiliy Nikolaevich Goral | Composite Substrate for 3D Cell Culture |
| CN202322856U (en) * | 2011-11-14 | 2012-07-11 | 江苏百奥特医疗仪器科技有限公司 | Cell culture device for high-flux high-resolution immunofluorescent staining |
| CN105331535B (en) * | 2015-11-05 | 2017-08-25 | 中国科学院电子学研究所 | Micro-fluidic chip and its application method for rebuilding osteocyte microenvironment |
| CN105861309B (en) * | 2016-04-14 | 2018-05-11 | 清华大学 | A kind of super-hydrophobic micro-pit array chip and preparation method and application |
-
2016
- 2016-04-14 CN CN201610232372.6A patent/CN105861309B/en active Active
-
2017
- 2017-03-31 WO PCT/CN2017/078946 patent/WO2017177839A1/en active Application Filing
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1434296A (en) * | 2003-03-04 | 2003-08-06 | 成都夸常科技有限公司 | Biological chip with minimized reactor isolation structure height and its preparation method |
| CN1858593A (en) * | 2006-03-23 | 2006-11-08 | 厦门大学 | Hydrophilic and phydrophobic mode sheet base special for biological chip |
| CN101481081A (en) * | 2009-01-21 | 2009-07-15 | 重庆大学 | Preparation of composite structure super-hydrophobic film |
| CN102439717A (en) * | 2009-03-24 | 2012-05-02 | 芝加哥大学 | Slip chip device and method |
| US20120302467A1 (en) * | 2010-11-29 | 2012-11-29 | Karlsruher Institut Fur Technologie | Patterned substrates for cell applications |
| CN103335984A (en) * | 2013-06-09 | 2013-10-02 | 清华大学 | Microarray chip without solid wall based on LSPR (Localized Surface Plasmon Resonance) and application thereof |
| CN103753984A (en) * | 2014-01-25 | 2014-04-30 | 深圳清华大学研究院 | Stamp, manufacturing method of stamp and manufacturing method of drop array |
| CN104762207A (en) * | 2015-04-24 | 2015-07-08 | 何向锋 | Special culture membrane for single cell cloning separation and single cell cloning separation method |
| CN104774763A (en) * | 2015-04-24 | 2015-07-15 | 何向锋 | Microchip type single cell cloning separation culture plate and single cell cloning separation method |
| CN105463075A (en) * | 2015-11-19 | 2016-04-06 | 北京科技大学 | Method for detecting miRNA based on super-hydrophilic micro-well sensing interface |
Non-Patent Citations (4)
| Title |
|---|
| DAVID K. WOOD等: "Single cell trapping and DNA damage analysis using microwell arrays", 《PNAS》 * |
| PENGFEI ZHANG等: "High-throughput superhydrophobic microwell arrays for investigating multifactorial stem cell niches", 《LAB ON A CHIP》 * |
| 林炳承著: "《微流控芯片实验室》", 31 December 2006 * |
| 王喆垚编著: "《信息、控制与系统技术丛书 微系统设计与制造》", 31 December 2015 * |
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