CN220812460U

CN220812460U - Wall-attached cell eluting and harvesting device

Info

Publication number: CN220812460U
Application number: CN202322550677.XU
Authority: CN
Inventors: 朱羚; 朱翔
Original assignee: Nanjing Birui Biotechnology Co ltd
Current assignee: Nanjing Birui Biotechnology Co ltd
Priority date: 2023-09-19
Filing date: 2023-09-19
Publication date: 2024-04-19
Anticipated expiration: 2033-09-19

Abstract

The application relates to an adherent cell elution harvesting device, which relates to the technical field of cell culture and comprises a base, a turntable, a connecting rod, a supporting rod, a universal ball, a culture bottle seat and a fixing rod, wherein the turntable can rotate relative to the base, one end of the connecting rod is rotationally connected to the peripheral position of the turntable, one end of the supporting rod is rotationally connected with the other end of the connecting rod, the other end of the supporting rod penetrates through the center of the universal ball and is fixedly connected with the universal ball, one end, far away from the connecting rod, of the supporting rod is fixedly provided with the culture bottle seat, and the universal ball is arranged in a ball socket structure of the fixing rod. The application has the technical effect of reducing the damage to adherent cells during elution and harvest.

Description

Wall-attached cell eluting and harvesting device

Technical Field

The application relates to the technical field of cell culture, in particular to an adherent cell eluting and harvesting device.

Background

Cell culture refers to a method for simulating in vitro in vivo environment to enable living, growing, propagating and maintaining main structures and functions, and when cell culture is carried out, part of cells can attach and spread on the wall of a culture flask to grow to form adherent cells. When the cell culture in the cell culture flask is completed, the adherent cells need to be eluted and harvested.

When the adherent cells are harvested, the cells are often promoted to fall off by means of external force, usually, digestive juice is used for infiltrating the cells, then the culture flask is manually and gently beaten, or the cells are separated from the wall of the culture flask by stirring, vibration and other modes and fall into the culture solution, so that the cells are conveniently obtained from the cell culture solution.

In the existing adherent cell collection mode, the bottle body is manually beaten or rocked, standardized and unified operation cannot be achieved due to different personal operations and different forces, cells cannot fall off from the bottle wall due to too small forces, the collection of the cells is incomplete, and the cells are easily damaged due to too large forces; stirring has a certain shearing force, and is easy to damage cells; vibration can promote loosening of the interface, so that the risk of cell contamination is brought, and cells in the culture flask are easily infected by bacteria to lose the original bioactivity.

Disclosure of utility model

In order to solve the problem that adherent cells are easy to damage during elution and harvesting, the application provides an adherent cell elution and harvesting device.

The application provides an adherent cell eluting and harvesting device which adopts the following technical scheme:

The utility model provides an adherent cell elution harvesting apparatus, includes base, carousel, connecting rod, bracing piece, universal ball and dead lever, the carousel can be relative rotate, connecting rod one end is rotated and is connected on the carousel periphery position, bracing piece one end with the connecting rod other end is rotated and is connected, the bracing piece other end passes universal ball center just the bracing piece with universal ball fixed connection, the one end of dead lever is provided with the ball socket, the universal ball is installed in the ball socket and can rotate in the ball socket, the dead lever with base fixed connection.

By adopting the technical scheme, the supporting rod is fixedly connected to the universal ball, and the supporting rod can rotate and shake through the rotation of the universal ball in the ball socket; the rotary table, the connecting rod and the supporting rod are connected, so that the supporting rod can be driven to smoothly rotate through rotation of the rotary table.

In a specific embodiment, the device further comprises a motor, wherein the motor is fixed on the base and is in driving connection with the turntable.

Through adopting above-mentioned technical scheme, utilize the motor to be connected with the carousel, can alleviate the dependence to manual operation to make the rotation of carousel more steady.

In a specific embodiment, a turntable support rod is provided on the turntable, the turntable support rod being articulated to the connecting rod.

Through adopting above-mentioned technical scheme, utilize carousel branch and connecting rod to articulate, make the articulated more convenient between carousel and the connecting rod.

In a specific implementation mode, a disc waist-shaped hole is formed in the whole body part of the disc body, and the rotary disc supporting rod is fixedly connected to the disc body through the disc waist-shaped hole.

Through adopting above-mentioned technical scheme, utilize the carousel kidney-shaped hole that sets up on the disk body, can fix the radial position of difference on the carousel with carousel branch, form the different turning radius of carousel branch to adjust the amplitude of rocking of bracing piece.

In a specific implementation manner, two ends of the connecting rod are provided with hinging blocks, two mutually perpendicular hinging shafts are arranged on the hinging blocks, one hinging block is hinged with the connecting rod and the turntable respectively through the two hinging shafts, and the other hinging block is hinged with the connecting rod and the supporting rod respectively through the two hinging shafts.

Through adopting above-mentioned technical scheme, utilize bracing piece and connecting rod and carousel branch to be connected respectively perpendicularly on the hinge piece for each hinge structure homoenergetic produces the rotation of different directions, can make between bracing piece and connecting rod and the carousel branch obtain more degree of freedom of rotation, avoids appearing the problem of jamming between bracing piece and connecting rod and the carousel branch.

In a specific embodiment, a protrusion is provided at one end of the connection between the support rod and the universal ball.

Through adopting above-mentioned technical scheme, utilize the arch of setting in bracing piece and universal ball one end, can inject the fixed position of universal ball on the bracing piece to obtain different radius of rotation and rocking range.

In a specific embodiment, the device further comprises a support plate fixedly connected with the base, and the fixing rod is fixed on the support plate.

Through adopting above-mentioned technical scheme, utilize dead lever and backup pad fixed connection, backup pad and base fixed connection's setting for the dead lever passes through the backup pad to be fixed on the base, can improve the fixed strength of dead lever, makes the device structure more stable at the during operation.

In a specific implementation manner, the supporting plate comprises a main plate and wing plates, the wing plates are fixedly connected to two sides of the main plate, the wing plates and the main plate are located on different planes, main plate kidney-shaped holes are formed in the vertical direction of the main plate, and the fixing rods are fixedly arranged on the main plate through the main plate kidney-shaped holes.

By adopting the technical scheme, the structure of the supporting plate is more stable by utilizing the wing plates fixedly connected to the main plate; the fixing rod can be fixed on different heights of the main board by utilizing the waist-shaped holes of the main board, so that the supporting rod fixedly connected with the universal ball has different rotation radiuses and swing amplitudes.

In a specific implementation manner, the device support rod is fixedly connected with the base, a waist-shaped hole of the device support rod is formed in the vertical direction of the device support rod, and the fixing rod is fixed on the device support rod through the waist-shaped hole of the device support rod.

Through adopting above-mentioned technical scheme, utilize the device bracing piece that is provided with device bracing piece kidney-shaped hole, can guarantee to improve the fixed strength of dead lever, make the device structure more stable at the during operation.

In a specific implementation manner, the cell bottle seat further comprises a supporting seat, movable brackets, a fixing plate, a buckle and a torsion spring, wherein the supporting seat is fixedly connected with the supporting rod and far away from one end of the connecting rod, the movable brackets are rotationally connected to the supporting seat, each movable bracket is far away from one end of the supporting seat and is provided with the fixing plate, the buckle is rotationally connected to one side edge of the fixing plate, the other side of the buckle, which is connected with the fixing plate, is provided with a bayonet bulge, the edge of the other side of the fixing plate is radially provided with a plurality of clamping grooves, the bayonet bulge can be clamped with the clamping grooves, the torsion spring is arranged at the joint of the buckle and the fixing plate, and rubber gaskets are arranged on the supporting seat and on the inner side of the fixing plate.

By adopting the technical scheme, the cell bottle seat can form the fixation of different types of cell culture bottles by utilizing the movable bracket rotationally connected to the supporting seat and the buckle on the fixed plate; by utilizing the rubber gaskets on the supporting seat and the fixing plate, the acting force between the cell culture bottle and the cell bottle seat, which are fixed on the cell bottle seat during the operation of the device, can be buffered, so as to prevent damage to the cell culture bottle.

In summary, the present application includes at least one of the following beneficial technical effects:

1. The rotary table is driven to rotate through the motor, so that the supporting rod is driven to rotate, the degree of freedom of the movement of the supporting rod relative to the fixed rod can be improved through the universal ball which is arranged in the ball socket structure and connected with the supporting rod, and the rotation and the shaking of the cell culture bottle which is fixed on the cell bottle seat on the supporting rod are formed, so that adherent cells on the cell bottle wall are eluted.

2. Through the arrangement of the cell bottle seat, different types of cell culture bottles can be fixed at the end parts of the support rods; the rubber gaskets on the supporting seat and the fixing plate can buffer the impact force received by the cell culture bottle in the shaking process, so that the vibration of the cell culture bottle is slowed down, and the damage of the cell culture bottle is prevented;

3. The shaking frequency of the cell bottle seat fixed on the supporting rod can be controlled by controlling the rotating speed of the motor rotating shaft, so that the problem that the cell bottle mouth is loosened due to too fast shaking of the frequency, and cell contamination is caused; the end part of the supporting rod is driven to do circular motion by utilizing the rotation of the turntable, so that the culture bottle can shake uniformly, thereby forming the scouring of the culture solution to the wall of the culture bottle, and avoiding the damage to cells caused by the shearing force generated in the culture solution; the fixed position of the turntable support rod in the waist-shaped hole of the turntable and the position of the fixed rod fixed on the support plate are adjusted, so that the shaking radius of the end part of the support rod connected with the universal ball is changed, the shaking amplitude of the cell culture bottle arranged at the other end of the support rod is controlled, the flowing speed of the culture solution in the culture bottle is in a proper interval, and the damage to cells is avoided while the good elution effect on adherent cells is ensured.

Drawings

FIG. 1 is a schematic diagram of an embodiment of an adherent cell eluting device of the present application.

FIG. 2 is a partial enlarged view of the culture flask holder in an embodiment of the adherent cells eluting device of the present application.

FIG. 3 is a cross-sectional view showing the connection between the buckle and the fixing plate in an embodiment of the adherent cell eluting device of the present application.

FIG. 4 is a schematic diagram of an adherent cell eluting device according to another embodiment of the present application.

Reference numerals illustrate: 1. a base; 2. a turntable; 21. a turntable support rod; 22. waist-shaped holes of the turntable; 3. a connecting rod; 4. a support rod; 41. a protrusion; 5. a universal ball; 6. a culture flask seat; 61. a support base; 62. a movable bracket; 63. a fixing plate; 631. a clamping groove; 64. a buckle; 641. a snap-fit protrusion; 65. a torsion spring; 7. a fixed rod; 71. a ball and socket structure; 8. a motor; 9. a support plate; 91. a main board; 911. a main plate kidney-shaped hole; 92. a wing plate; 10. and a supporting rod is arranged.

Detailed Description

The following describes specific embodiments of the present application in detail with reference to the drawings. It should be understood that the detailed description and specific examples, while indicating and illustrating the application, are not intended to limit the application.

In the description of the present application, it should be noted that, unless explicitly stated and limited otherwise, the terms "disposed" and "connected" should be interpreted broadly, and for example, they may be fixedly connected, detachably connected, or integrally connected; either directly or indirectly via an intermediate medium, or in communication with each other or in interaction with each other. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances.

The embodiment of the application discloses an adherent cell eluting device.

Example 1

As shown in fig. 1, one embodiment of the adherent cell eluting device of the present application includes a base 1, a turntable 2, a link 3, a support bar 4, a universal ball 5, a flask holder 6, a fixing bar 7, a motor 8, and a support plate 9. The base 1 can be made of rectangular metal plates to form an installation foundation of the eluting device, a through hole is formed in the base 1, the motor 8 is fixed on the base 1 through bolts and located below the through hole, and a rotating shaft of the motor 8 is fixedly connected with the rotary table 2 arranged on the other side of the base 1 through the through hole in the base 1. The turntable 2 is a circular metal disk, a turntable waist-shaped hole 22 is arranged on the upper body part of the turntable 2, and the turntable waist-shaped hole 22 is arranged along the radial direction of the turntable 2. The turntable 2 is provided with a turntable support rod 21, the turntable support rod 21 is rod-shaped or tubular and made of metal materials, the lower end of the turntable support rod 21 is fixed in the turntable kidney-shaped hole 22 through a screw, and the fixed position of the turntable support rod 21 in the turntable kidney-shaped hole 22 can be adjusted, so that when the turntable 2 rotates, different rotation radiuses of the turntable support rod 21 can be formed.

The connecting rod 3 is rod-shaped or tubular and is made of metal, the two ends of the connecting rod 3 are provided with hinge blocks, the hinge blocks are made of metal, two hinge shafts are arranged on the hinge blocks, the two hinge shafts are mutually perpendicular, the hinge blocks at one end of the connecting rod 3 are respectively hinged with the turntable support rod 21 and the connecting rod 3 through the two hinge shafts, and the hinge blocks at the other end of the connecting rod 3 are respectively hinged with the connecting rod 3 and the supporting rod 4 through the two hinge shafts. The support rod 4 is rod-shaped or tubular and is typically made of a metallic material. The universal ball 5 can be a metal sphere, a through hole is arranged at the sphere center of the universal ball 5, one end of the supporting rod 4 far away from the hinge joint penetrates through the universal ball 5 through the through hole, the fixing position of the universal ball 5 on the supporting rod 4 is limited by utilizing the bulge 41 on the supporting rod 4, and the universal ball 5 is fixed on the supporting rod 4. The culture flask seat 6 is fixed at the end of the support rod 4 far away from one end of the base 1 and is used for installing and fixing a cell culture flask. The fixing rod 7 is rod-shaped or tubular and made of metal, one end of the fixing rod 7 is provided with a hollow ball socket structure 71 with openings at the upper end and the lower end, the universal ball 5 is arranged in the ball socket structure 71 and can rotate in the ball socket structure 71, and the other end of the fixing rod 7 is fixedly connected to the supporting plate 9 through a screw. The support plate 9 may be replaced by a support rod.

The backup pad 9 includes mainboard 91 and pterygoid lamina 92, and mainboard 91 can set to "L" metal sheet, and pterygoid lamina 92 sets up in the both sides of mainboard 91, and is in different planes with mainboard 91, and pterygoid lamina 92 preferably sets up in the vertical position of mainboard 91, sets up mainboard kidney-shaped hole 911 on the vertical direction of mainboard 91, and dead lever 7 can pass the different positions and mainboard 91 fixed connection of mainboard kidney-shaped hole 911 through the screw, backup pad 9 and base 1 fixed connection, can adopt welded, bonding or fastener connection etc. connection mode fixed connection between the two. As shown in fig. 2, the culture flask holder 6 includes a support holder 61, a movable support 62, a fixing plate 63, a buckle 64 and a torsion spring 65, the support holder 61 may be a circular metal block or a circular metal ring, one end of the support holder 61 is welded at the end of the support rod 4, the other end is provided with a rubber gasket, when the cell culture flask is fixed, the bottom of the cell culture flask is contacted with the rubber gasket, the damage to the cell culture flask caused by the support holder 61 during operation is prevented, the support holder 61 is rotatably connected with a plurality of movable supports 62, preferably three movable supports 62 are provided, each movable support 62 is provided with a fixing plate 63, the movable support 62 is fixed at the outer side of the fixing plate 63, and the rubber gasket is provided at the inner side of the fixing plate 63. The fixed plate 63 one side rotates and is connected with buckle 64, and torsional spring 65 sets up in the junction of fixed plate 63 and buckle 64, and torsional spring 65 one end supports and leans on fixed plate 63, and the other end supports and leans on buckle 64 to drive buckle 64 rotation and adjacent fixed plate 63 support and lean on, fixed plate 63 opposite side is provided with draw-in groove 631, and buckle 64 keeps away from the one end of being connected with fixed plate 63 rotation and is provided with buckle protruding 641, and buckle 64 can be connected on adjacent fixed plate 63 through the block of buckle protruding 641 and draw-in groove 631.

Example 2

As shown in fig. 4, this embodiment is different from embodiment 1 in that: through setting up device bracing piece 10, set up device bracing piece kidney-shaped hole on the device bracing piece 10, make dead lever 7 pass the different positions of device bracing piece kidney-shaped hole and device bracing piece 10 fixed connection through the screw to with base 1 fixed connection.

The implementation principle of the adherent cell elution harvesting device of the application is as follows: when the harvesting device is in operation, the cell culture flask is placed on the support seat 61 of the culture flask seat 6, the movable bracket 62 is connected with the support seat 61 in a rotating way, the clamping protrusion 641 on the clamping buckle 64 is clamped in the clamping groove 631 on the adjacent fixing plate 63, and the cell culture flask is surrounded and fixed in the culture flask seat 6. The elastic force of the torsion spring 65 to the fixing plate 63 and the buckle 64 is beneficial to maintaining the clamping state between the clamping groove 631 and the buckle protrusion 641. The snap projection 641 is engaged with a different snap groove 631, so that cell culture flasks of different sizes can be fixed. The starting motor 8 rotates to drive the turntable 2 to rotate, and when the turntable 2 rotates, the turntable support rod 21 is driven to rotate, the turntable support rod 21 is hinged with the connecting rod 3 to drive the connecting rod 3 to rotate, one end of the supporting rod is hinged with the supporting rod 4 to enable the other end of the supporting rod 4 to do circular motion, the supporting rod 4 is enabled to be provided with one end of the cell bottle seat to shake through rotation of the universal ball 5 in the ball socket structure 71, so that the cell culture bottle arranged on the culture bottle seat 6 rotates and shakes, the culture solution flows back and forth in the culture bottle, and cells growing on the wall of the bottle are washed, so that the cells fall into the culture solution, and the adherent cells in the culture solution are harvested. The rotating speed of the motor is changed, so that the rotating speed of the supporting rod 4 can be controlled, the shaking frequency of the cell culture bottle is in a proper interval, and the risk of cell contamination caused by loosening of a bottle opening due to high-frequency vibration of the cell culture bottle is prevented; by changing the position of the turntable support rod 21 fixed on the turntable 2 and the position of the fixing rod 7 fixed on the support plate 9 or the support rod 10 of the device, the shaking radius of the support rod 4 connected with the universal ball 5 is changed, the shaking amplitude of the cell culture bottle fixed on the support rod 4 in the culture bottle seat 6 is controlled, the flowing speed of the culture solution in the culture bottle is in a proper interval, and adherent cells on the wall of the cell culture bottle can be eluted and harvested under the condition of avoiding damage to the cells.

The above embodiments are not intended to limit the scope of the present application, so: all equivalent changes in structure, shape and principle of the application should be covered in the scope of protection of the application.

Claims

1. An adherent cell elution harvesting device, characterized in that: including base (1), carousel (2), connecting rod (3), bracing piece (4), universal ball (5) and dead lever (7), carousel (2) set up to can be relative base (1) rotates, connecting rod (3) one end with carousel (2) periphery articulates mutually, the other end with bracing piece (4) articulates mutually, bracing piece (4) other end passes universal ball (5) center with universal ball (5) fixed connection, the one end of dead lever (7) is provided with ball socket structure (71), universal ball (5) are installed in ball socket structure (71) and can rotate in ball socket structure (71), dead lever (7) with base (1) fixed connection.

2. The adherent cell eluting harvesting device of claim 1, wherein: the rotary table also comprises a motor (8), wherein the motor (8) is fixed on the base (1) and is in driving connection with the rotary table (2).

3. The adherent cell eluting harvesting device of claim 1, wherein: a turntable support rod (21) is arranged on the turntable (2), and the turntable support rod (21) is hinged with the connecting rod (3).

4. An adherent cell eluting harvesting device according to claim 3, wherein: the whole body part of the rotary table (2) is provided with a rotary table kidney-shaped hole (22), and the rotary table support rod (21) is fixedly connected to the rotary table (2) through the rotary table kidney-shaped hole (22).

5. The adherent cell eluting harvesting device of claim 1, wherein: two ends of the connecting rod (3) are respectively provided with a hinging block, two hinging shafts perpendicular to each other are arranged on the hinging blocks, one hinging block is hinged with the connecting rod (3) and the rotary table (2) respectively through the two hinging shafts, and the other hinging block is hinged with the connecting rod (3) and the supporting rod (4) respectively through the two hinging shafts.

6. The adherent cell eluting harvesting device of claim 1, wherein: one end of the joint of the supporting rod (4) and the universal ball (5) is provided with a bulge (41).

7. The adherent cell eluting harvesting device of claim 1, wherein: the novel portable electric power tool further comprises a supporting plate (9), wherein the supporting plate (9) is fixedly connected with the base (1), and the fixing rod (7) is fixed on the supporting plate (9).

8. The adherent cell eluting harvesting device of claim 7, wherein: the support plate (9) comprises a main plate (91) and wing plates (92), the wing plates (92) are fixedly connected to two sides of the main plate (91), the wing plates (92) and the main plate (91) are located on different planes, main plate kidney-shaped holes (911) are formed in the vertical direction of the main plate (91), and the fixing rods (7) are fixedly arranged on the main plate (91) through the main plate kidney-shaped holes (911).

9. The adherent cell eluting harvesting device of claim 8, wherein: the device support rod (10) is fixedly connected with the base (1), a device support rod kidney-shaped hole is formed in the vertical direction of the device support rod (10), and the fixing rod (7) is fixed on the device support rod (10) through the device support rod kidney-shaped hole.

10. The adherent cell eluting harvesting device of claim 1, wherein: still include blake bottle seat (6), blake bottle seat (6) include supporting seat (61), movable support (62), fixed plate (63), buckle (64) and torsional spring (65), supporting seat (61) fixed connection is in bracing piece (4) are kept away from connecting rod (3) one end, set up a plurality of on supporting seat (61) movable support (62), keep away from at every movable support (62) supporting seat (61) one end is provided with fixed plate (63), buckle (64) rotate connect fixed plate (63) one side edge position department buckle (64) go up with the opposite side that fixed plate (63) are connected is provided with buckle arch (641), fixed plate (63) opposite side edge radially is provided with a plurality of draw-in grooves (631), buckle arch (641) can with draw-in groove (631) looks block, torsional spring (65) set up buckle (64) with the junction of fixed plate (63), on supporting seat (61) with fixed plate (63) inboard is provided with gasket (63).