CN220812447U - Visual inspection device for cell growth state - Google Patents

Abstract

The present specification provides a visual inspection apparatus for cell growth status. The device comprises at least: a cell culture vessel, a light source, and a base structure; wherein the cell culture vessel is arranged at the upper position of the base structure; the cell culture vessel is a transparent cell culture vessel; the light source is arranged at the upper position of the cell culture vessel; the base structure at least comprises a displacement table and a microscopic imaging component; wherein, the microscopic imaging component is connected with the displacement table; an observation window is also arranged at the position where the base structure is connected with the cell culture vessel; the microscopic imaging component is arranged at the lower position of the observation window; the displacement platform at least comprises an optical axis movement module; the base structure is also connected with the processor; the processor is connected with the displacement table and the microscopic imaging component; based on the device, the automatic focusing before the use of the visual detection device for the cell growth state can be efficiently and accurately realized, the operation of a user side is effectively simplified, and the operation threshold of the user is reduced.

Description

Visual inspection device for cell growth state

Technical Field

The specification belongs to cell culture equipment technical field, especially relates to cell growth state visual detection equipment.

Background

During cell culture, it is often necessary to observe the growth state of cells at random.

Based on the existing detection equipment, most users need to manually perform focusing operation before using the detection equipment; after the focusing operation is completed, the user can observe the growth state of the cells by using the detection device normally. However, the manual focusing process is relatively complex and cumbersome; and a great deal of dependence on personal experience of the user and familiarity of the person with the detection device are required, so that a certain operation threshold is provided for many users, and many users often cannot efficiently and accurately complete focusing of the detection device.

Aiming at the problems, the existing cell growth state detection equipment at present cannot be effectively solved.

Disclosure of utility model

The specification provides a cell growth state visual inspection device, can high-efficient, accurately realize the automatic focus before cell growth state visual inspection device uses, simplify user side operation effectively, reduce user's operation threshold.

The present specification provides a visual inspection apparatus for cell growth status, comprising at least: a cell culture vessel, a light source, and a base structure; wherein the cell culture vessel is arranged at the upper position of the base structure; the cell culture vessel is a transparent cell culture vessel; the light source is arranged at the upper position of the cell culture vessel; the cell culture vessel is used for containing cells to be cultured;

The base structure at least comprises a displacement table and a microscopic imaging component; wherein the microscopic imaging component is connected with the displacement table; an observation window is also arranged at the position where the base structure is connected with the cell culture vessel; the microscopic imaging component is arranged at the lower position of the observation window;

the displacement table at least comprises an optical axis movement module; the base structure is also connected with the processor; the optical axis movement module is used for driving the microscopic imaging component to move along the optical axis direction;

The processor is connected with the displacement table and the microscopic imaging component; the processor is used for sending corresponding control instructions to the displacement table and the microscopic imaging assembly to realize automatic focusing of the visual cell growth state detection equipment.

In one embodiment, further comprising: a light source controller; wherein, the light source controller is respectively connected with the light source and the processor.

In one embodiment, a light sensor is provided adjacent to the microimaging assembly; the light sensor is connected with the processor.

In one embodiment, a range finder is also provided adjacent to the microscopic imaging assembly; the distance meter is connected with the processor.

In one embodiment, the displacement table is provided with a connection plate; the microscopic imaging component is arranged at the upper position of the connecting plate; and a telescopic component is further arranged between the microscopic imaging component and the connecting plate.

In one embodiment, the device further comprises a light source support; the light source is arranged at the upper position of the cell culture vessel through the light source support.

In one embodiment, the light source holder comprises at least a telescopic rod.

In one embodiment, the light source support further comprises a telescoping control motor; the telescopic control motor is electrically connected with the telescopic rod and the light source controller respectively.

In one embodiment, the optical axis movement module includes at least: an optical axis motor, a positioning shaft and an output shaft connecting block; wherein, the go out axle connecting block with microscopic imaging subassembly links to each other.

In one embodiment, the microimaging assembly comprises at least: a micro lens and a camera; wherein, the microscope lens and the camera are connected through threads.

The cell growth state visual inspection device provided based on the specification at least comprises: a cell culture vessel, a light source, and a base structure; wherein the cell culture vessel is arranged at the upper position of the base structure; the cell culture vessel is a transparent cell culture vessel; the light source is arranged at the upper position of the cell culture vessel; the base structure at least comprises a displacement table and a microscopic imaging component; wherein, the microscopic imaging component is connected with the displacement table; an observation window is also arranged at the position where the base structure is connected with the cell culture vessel; the microscopic imaging component is arranged at the lower position of the observation window; the displacement platform at least comprises an optical axis movement module; the base structure is also connected with the processor; the processor is connected with the displacement table and the microscopic imaging component; based on the device, the automatic focusing of the visual detection device for the cell growth state can be accurately and efficiently realized. In the implementation, the processor can send corresponding motion control instructions to the displacement platform, so that the displacement platform can automatically drive a microscopic imaging assembly comprising a microscopic lens and a camera to move along the optical axis direction through the optical axis motion module; and a corresponding acquisition control instruction is sent to the microscopic imaging assembly, so that the microscopic imaging assembly automatically acquires corresponding test images at different positions along the optical axis direction; the processor receives a plurality of test images which are transmitted by the microscopic imaging component and are acquired at different positions; and the processor is used for realizing the automatic focusing of the visual detection equipment for the cell growth state by finding out the image acquisition position which meets the requirements and is suitable for image acquisition according to the plurality of test images.

Drawings

In order to more clearly illustrate the embodiments of the present disclosure, the drawings that are required for the embodiments will be briefly described below, and the drawings described below are only some embodiments described in the present disclosure, and other drawings may be obtained according to these drawings without inventive effort for a person of ordinary skill in the art.

FIG. 1 is a schematic structural view of a visual inspection apparatus for cell growth status according to one embodiment of the present disclosure;

FIG. 2 is a schematic structural view of a visual inspection apparatus for cell growth status according to another embodiment of the present disclosure;

FIG. 3 is a schematic structural view of a visual inspection apparatus for cell growth status according to still another embodiment of the present disclosure;

fig. 4 is a schematic diagram showing the partial structural composition of a base structure in a visual inspection apparatus for cell growth status according to an embodiment of the present disclosure.

Detailed Description

In order to make the technical solutions in the present specification better understood by those skilled in the art, the technical solutions in the embodiments of the present specification will be clearly and completely described below with reference to the drawings in the embodiments of the present specification, and it is obvious that the described embodiments are only some embodiments of the present specification, not all embodiments. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are intended to be within the scope of the present disclosure.

Referring to fig. 1, an embodiment of the present disclosure provides a visual inspection apparatus for cell growth status, at least including: a cell culture vessel, a light source, and a base structure; wherein the cell culture vessel is arranged at the upper position of the base structure; the cell culture vessel is a transparent cell culture vessel; the light source is arranged at the upper position of the cell culture vessel; the cell culture vessel is used for containing cells to be cultured;

The base structure may include at least a displacement table, a microimaging assembly, etc.; the micro lens is connected with the camera and then connected with the displacement table; an observation window is also arranged at the position where the base structure is connected with the cell culture vessel; the microscope lens and the camera are arranged at the lower position of the observation window; the microscopic imaging assembly may specifically include an associated microscope lens and camera.

The displacement table at least comprises an optical axis movement module; the base structure may also be coupled to the processor; the optical axis movement module is used for driving the microscopic imaging component to move along the optical axis direction; wherein, the optical axis operation module at least comprises an optical axis motor.

The processor is connected with the displacement table and the microscopic imaging component; the processor is used for sending corresponding control instructions to the displacement table and the microscopic imaging assembly to realize automatic focusing; and/or visual detection of the growth status of cells in the cell culture vessel.

Based on the cell growth state visual detection equipment, when the device is implemented, the processor can enable the optical axis movement module in the displacement platform to automatically drive the microscopic imaging component to move along the optical axis (for example, Z axis) direction by sending a corresponding movement control instruction to the displacement platform; the corresponding acquisition control instruction is sent to the microscopic imaging assembly, so that the microscopic imaging assembly automatically acquires a plurality of test images at different positions along the optical axis direction; the processor receives a plurality of test images which are transmitted by the microscopic imaging component and are collected at different positions; and the processor determines the image acquisition position meeting the requirements according to the plurality of test images, and realizes the automatic focusing of the visual detection equipment for the cell growth state.

In addition, the processor can also send a corresponding acquisition control instruction to the microscopic imaging assembly at a corresponding time point, so that the microscopic imaging assembly can acquire a cell image containing cells in the cell culture vessel through the transparent cell culture vessel based on the image acquisition position determined by the automatic focusing before on the premise of not opening the cell culture vessel at the corresponding time point; the processor receives the cell image acquired by the microscopic imaging component; and visual detection of the growth state of cells in the cell culture vessel is realized according to the cell image.

Wherein, the cell culture vessel can be made of transparent material. Specifically, the cell culture vessel may be a T-flask, a circular culture vessel, or a culture vessel with other suitable shapes such as a well plate culture vessel.

The optical axis movement module (e.g., Z-axis movement module) supports movement in the optical axis (e.g., Z-axis) direction.

In some embodiments, the microimaging assembly can include at least: microscope lens, camera, etc.; wherein, the microscope lens and the camera are connected through threads.

Specifically, an internal thread is arranged on the outer side of the interface of the microscope lens; and matched external threads are arranged on the inner side of the camera interface. When the micro imaging assembly is specifically connected, the interface of the micro lens can be screwed into the camera interface based on the matched threads so as to connect the micro lens with the camera to obtain the corresponding micro imaging assembly.

Furthermore, when the microscope lens is connected with the camera, the distance between the microscope lens and the camera can be adjusted by controlling the screw thread quantity of the microscope lens interface screwed into the camera interface according to specific conditions and observation requirements, so as to obtain an observation field of view meeting the requirements of diversified users.

In some embodiments, the displacement table is provided with a connection plate; the microscopic imaging assembly may be specifically disposed at an upper position of the connection plate. Correspondingly, the displacement platform can drive the connecting plate to move along the optical axis direction through the optical axis movement module, so as to drive the microscopic imaging component arranged on the connecting plate to move correspondingly.

In some embodiments, the viewing window may be made of a transparent material (e.g., high-transmittance glass). Correspondingly, the light signals emitted by the light source can sequentially pass through the cell culture vessel and the observation window to reach the microscope lens and the camera, so that the microscope lens and the camera can acquire the cell image based on the microscopic level.

In some embodiments, the cell growth status visual inspection apparatus may specifically further include: a light source controller; wherein, the light source controller is respectively connected with the light source and the processor.

In particular implementations, the processor may generate and send light source adjustment control instructions to the light source controller. The light source controller receives and responds to the light source adjustment control instruction to correspondingly adjust the light source.

In some embodiments, a light sensor is also provided adjacent to the microscope lens; the light sensor is connected with the processor.

In the implementation, referring to fig. 1, a light sensor may be disposed on the connection board at a position adjacent to the microscope lens. The light sensor can monitor the brightness signal of the light source in real time and send the brightness signal of the light source to the processor. The processor determines that the current light source is darker when determining that the brightness signal is smaller than or equal to a preset brightness signal threshold value according to the brightness signal of the light source; at this time, a light source adjustment control instruction regarding increasing the brightness of the light source may be generated and transmitted to the light source controller. Correspondingly, the light source controller receives and responds to the light source adjustment control instruction, and automatically adjusts the light source to improve the light source brightness.

Conversely, when the brightness signal is determined to be larger than the preset brightness signal threshold value, the current light source is determined to be brighter according to the brightness signal of the light source; at this time, a light source adjustment control instruction regarding lowering the brightness of the light source may be generated and transmitted to the light source controller. Correspondingly, the light source controller receives and responds to the light source adjustment control instruction, and adjusts the light source to reduce the light source brightness. Therefore, different bright environments can be intelligently identified and distinguished, and the control and adjustment of the brightness of the light source can be automatically realized, so that the acquired cell image is ensured to have better definition and higher image quality.

In some embodiments, referring to fig. 2, a range finder is further provided adjacent to the microscopic imaging assembly; the distance meter is connected with the processor. The distance meter can be a laser distance meter or an ultrasonic distance meter.

When the processor sends a corresponding motion control instruction to the displacement platform, so that an optical axis motion module in the displacement platform can automatically drive the microscopic imaging component to move to a corresponding target position along the direction of an optical axis (for example, a Z axis), and the processor can acquire the relative distance between the current actual position and the observation window by controlling the range finder; determining the actual position of the current microscopic imaging assembly along the optical axis direction according to the relative distance; and comparing the actual position with the target position to determine whether the deviation value between the current actual position and the expected target position is larger than a preset error tolerance threshold.

Under the condition that the deviation value between the current actual position and the target position is larger than the preset error tolerance threshold value, the processor generates and sends a corresponding fine adjustment control instruction to the displacement platform, so that an optical axis movement module in the displacement platform can receive and respond to the fine adjustment control instruction, and automatically drives the microscopic imaging assembly to perform relatively tiny adjustment movement along the optical axis direction, so that the microscopic imaging assembly can accurately reach the target position, and automatic focusing can be completed more accurately.

In some embodiments, the displacement table is provided with a connection plate; the microscopic imaging component is arranged at the upper position of the connecting plate; and a telescopic component is further arranged between the microscopic imaging component and the connecting plate.

The telescopic component can be electrically connected with the optical axis motor.

After the automatic focusing is completed and the proper image acquisition position is determined, when the processor sends a corresponding motion control instruction to the displacement platform, the optical axis motion module in the displacement platform can automatically drive the microscopic imaging assembly to move to the corresponding image position along the optical axis direction, and the processor can also generate and send a micro telescopic instruction to the optical axis motor; the optical axis motor receives and responds to the micro telescopic instruction, and the telescopic component is controlled to stretch and retract in a small extent, so that the microscopic imaging component is driven to move in a small extent along the optical axis direction based on the current image acquisition position, the micro adjustment of the microscopic imaging component along the optical axis direction is realized, the image acquisition position with relatively better and more accurate effect can be found, and the cell image is acquired.

In some embodiments, referring to fig. 2, the visual inspection apparatus for cell growth status may specifically further include a light source bracket; the light source is arranged at the upper position of the cell culture vessel through the light source support.

In a specific implementation, the light source may be specifically disposed at an upper position of the cell culture vessel by a light source support. Specifically, the light source bracket supports up-and-down displacement adjustment; correspondingly, the relative distance between the light source and the cell culture vessel can be adjusted along the vertical direction by adjusting the light source bracket. In addition, the light source bracket also supports the adjustment of left-right front-back displacement; correspondingly, the left-right relative distance and/or the front-back relative distance between the light source and the microscopic imaging component can be adjusted in the same plane by adjusting the light source bracket.

In some embodiments, the light source holder may include at least a telescoping rod. Correspondingly, the relative distance between the light source and the cell culture vessel can be adjusted by controlling the telescopic state of the telescopic rod of the light source bracket.

In some embodiments, the light source support may specifically further include a telescoping control motor; the telescopic control motor can be specifically and respectively electrically connected with the telescopic rod and the light source controller.

In the implementation, the light source controller can automatically adjust the relative distance between the light source and the cell culture vessel along the vertical direction by the telescopic control motor according to specific environment conditions or specific requirements of users, so that the acquired cell image has higher image quality.

In some embodiments, referring to fig. 3, the base structure is further provided with a communication interface; correspondingly, the displacement table, the camera and the micro lens are connected with the processor through the communication interface. The processor may be a computer device.

Specifically, a communication interface can be provided on a side wall of the housing of the base structure, so as to facilitate electrical connection between the processor and the displacement table inside the base structure, and between the camera and the microscope lens in the microscopic imaging assembly.

In some embodiments, the processor may be further specifically coupled to a display; the processor is also internally provided with an image acquisition card and a motion control card. Correspondingly, the processor can generate and send corresponding acquisition control instructions to the microscopic imaging component based on the image acquisition card; the processor may generate and send corresponding motion control instructions to the displacement station based on the motion control card described above. The display can be used for displaying the cell images acquired by the microscope lens and the camera to a user so as to display an interactive interface for the operation of the user.

Specifically, for example, when the visual inspection device performs auto-focusing based on the growth state, the processor may first send an initial acquisition control instruction to the microscope lens and the camera; and receiving initial images acquired and fed back by the microscope lens and the camera in response to the initial acquisition control instruction. The processor may identify and determine a cell type of the cells in the cell culture vessel based on the initial image; and screening out a target focusing strategy matched with the cell type from a preset focusing strategy set. The target focusing strategy specifically may include a plurality of reference positions and matched target evaluation rules. Wherein different reference positions are spaced apart from the target cell by different distances along the optical axis direction.

Correspondingly, the processor can determine a plurality of reference positions according to a target focusing strategy and generate a motion control instruction aiming at the optical axis motion module of the displacement platform; wherein the motion control instruction carries at least one reference position. And the optical axis movement module receives and responds to the movement control instruction, drives the micro lens and the camera to move to the corresponding reference positions along the optical axis direction, and sends confirmation information of movement completion to the processing module. The processor receives and responds to the confirmation information, and generates and sends corresponding test acquisition control instructions to the camera and the microscope lens. The microscope lens and the camera receive and respond to the test acquisition control instruction, and acquire a cell image containing cells in the cell culture vessel based on the current reference position as a test image; and sends the test image to the processor.

The processor receives a plurality of test images; wherein each test image corresponds to a reference position; the processor evaluates the plurality of test images according to the target evaluation rule to obtain a plurality of evaluation results; and determining an image acquisition position meeting the requirements from the multiple reference positions according to the evaluation result and the multiple reference positions, and realizing automatic focusing of the visual detection equipment for the cell growth state.

In some embodiments, the displacement stage includes at least an optical axis movement module. Specifically, referring to fig. 4, the optical axis movement module may at least include: optical axis motor, positioning shaft, output shaft connecting block, etc.; wherein, the go out axle connecting block with microscopic imaging subassembly links to each other.

Specifically, referring to fig. 4, the output shaft connection module may be specifically connected to a camera mounting board; the camera mounting plate is connected with the connecting plate, and the microscopic imaging component is specifically and fixedly arranged on the connecting plate.

When the optical axis movement module specifically operates, the optical axis motor is started, and the output shaft connecting block can be driven to reciprocate along the optical axis direction based on the positioning shaft; the output shaft connecting block drives the connecting plate to move together through the camera mounting plate while moving, and drives the microscopic imaging component arranged on the connecting plate to do reciprocating motion along the optical axis direction. Thereby can drive the microscopic imaging subassembly that microscope lens and camera on the connecting plate constitute and realize along the removal of optical axis direction

In particular, as shown in fig. 4, the optical axis motor may be specifically disposed on the motor mounting plate and connected to the output shaft connecting block through a related structure such as a positioning shaft connecting plate.

In some embodiments, the displacement stage may be a displacement stage supporting three-axis precision motion.

Specifically, the displacement table may further include a transverse axis movement module (e.g., an X-axis movement module) in addition to the optical axis movement module; wherein, the horizontal axis motion module at least includes: the device comprises a transverse motor, a transverse driving wheel, a first transverse driven wheel, a second transverse driven wheel, a first transverse driven shaft, a second transverse driven shaft and a transverse positioning shaft.

Wherein the lateral axis motion module supports movement in a lateral axis (e.g., X-axis) direction on a plane.

Specifically, the transverse axis movement module can drive the micro lens and the camera on the connecting plate to move along the transverse axis direction on the plane through combining the structures of the transverse axis motor, the transverse axis driving wheel, the first transverse axis driven wheel, the second transverse axis driven wheel, the first transverse axis driven shaft, the second transverse axis driven shaft, the transverse axis positioning shaft and the like.

Specifically, the displacement table may further include a longitudinal axis movement module; wherein the longitudinal axis movement module is arranged at the upper position of the transverse axis movement module; the longitudinal axis motion module comprises at least: the device comprises a longitudinal shaft motor, a longitudinal shaft driving wheel, a longitudinal shaft positioning shaft and a longitudinal shaft driven wheel.

Wherein the longitudinal axis motion module supports movement in a longitudinal axis (e.g., Y-axis) direction in a plane.

Specifically, the transverse axis movement module can drive the microscope lens and the camera on the connecting plate to move along the longitudinal axis direction on the plane by combining the structures of the longitudinal axis motor, the longitudinal axis driving wheel, the longitudinal axis positioning shaft, the longitudinal axis driven wheel and the like.

In some embodiments, a limit portion of the cell culture vessel may be further provided at a designated position of the observation window.

Specifically, the limiting part can be a right-angle positioning seat, and the limiting part can be well adapted to a plurality of different cell culture vessels and is used for limiting the placing position of the cell culture vessels on the observation window.

Further, the above-mentioned limiting portion may be specifically disposed at a corner position of the observation window, where the corner position may specifically be a zero point position of a horizontal axis and a vertical axis on a plane.

Specifically, inside the base structure, the stator portion of the transverse axis motion module may be specifically installed in the base structure, and specifically includes: the transverse motor, the transverse driving wheel, the transverse first driven shaft, the transverse positioning shaft, the transverse second driven wheel and the transverse second driven shaft. Wherein, each driving wheel and each driven wheel can be driven by a conveyor belt, and the transverse shaft motor is connected with the processor and has closed loop feedback of position and speed. The layout structure can effectively reduce the overall size of the transverse axis motion module and reduce the occupation of the internal space of the base structure.

The stator part of the vertical axis movement module can be specifically arranged on the horizontal axis movement module, and correspondingly, the vertical axis movement module can move along the horizontal axis direction along with the horizontal axis movement module; while providing power drive in the longitudinal direction, including in particular: the device comprises a longitudinal shaft motor, a longitudinal shaft driving wheel, a longitudinal shaft positioning shaft and a longitudinal shaft driven wheel. The longitudinal axis motor is connected with the processor.

The stator part of the optical axis movement module can be fixedly arranged on the longitudinal axis movement module through a motor mounting plate, and correspondingly, the optical axis movement module can move along the longitudinal axis direction along with the longitudinal axis movement module; while providing power drive in the direction of the optical axis. The optical axis motor is connected with the processor.

Finally, a connection plate provided with a micro lens and a camera can be arranged on the optical axis movement module. Therefore, the microscope lens and the camera can be driven by the optical axis movement module, the transverse axis movement module and the longitudinal axis movement module to move along the optical axis, the transverse axis and the longitudinal axis.

Specifically, a microscopic imaging component consisting of a microscopic lens and a camera can move along the directions of a transverse axis and a longitudinal axis along with the longitudinal axis movement module and the transverse axis movement module, so that mobile scanning based on a two-dimensional plane is realized, and cell images in various visual fields are acquired; meanwhile, the micro lens and the camera can also move along the optical axis direction along with the optical axis movement module, so as to realize automatic focusing and automatic focusing.

In some embodiments, the cell growth status visual inspection apparatus may specifically further include an auxiliary light source. The auxiliary light source can be arranged at a position where the light source bracket is adjacent to the light source.

Further, the auxiliary light source may be specifically connected to a light source controller. In the implementation, under the condition that the specific brightness of the light environment provided by the light source is not in accordance with the requirement, the light source controller can control the auxiliary light source to start so as to cooperate with the original light source to provide the light environment which is in accordance with the requirement for the growth state visual detection equipment.

Specifically, the processor can receive and determine the view position coordinate which the user wants to acquire according to the cell image acquisition requirement input by the user; and generating a corresponding movement control instruction, wherein the movement control instruction can carry the visual field position coordinates. The processor sends the movement control instruction to the horizontal axis movement module and the vertical axis movement module. The transverse axis movement module and the longitudinal axis movement module receive and respond to the movement control instruction to drive the microscope lens and the camera to move to the corresponding visual field positions; acknowledgement of the completion of the movement is generated and sent to the processor. And after receiving the confirmation information, the processor generates and sends an acquisition control instruction to the microscope lens and the camera. The microscope lens and the camera receive and respond to the acquisition control instruction to acquire a cell image based on the visual field; and sends the cell image to a processor. The processor receives the cell image and presents the cell image to a user via the display. In addition, the processor can also determine the growth state data of the cells, such as the cell growth stage, the cell survival rate, the cell density, the cell morphology and the like, by processing the cell image according to a built-in image processing algorithm.

Specifically, the processor may further receive and determine information such as a coordinate of a view position to be scanned, a number of views to be scanned, and the like according to user-defined scanning area information input by a user; and then a corresponding movement control instruction can be generated according to the information, and the movement control instruction is sent to the displacement platform. And the displacement platform receives and drives the microscope lens and the camera to move to corresponding visual field position coordinates through the corresponding motion module according to the movement control instruction, and acquires the cell image of the corresponding visual field. By performing the field scan in the above manner, the processor may receive a plurality of cell images, e.g., 4, 9, 30, etc., for the field acquired by the microscope lens and the camera at a plurality of different field position coordinates. The processor may stitch the plurality of cell images according to the corresponding view coordinate positions to obtain a cell image including a plurality of views at the same time, for example, a cell image including 4 views, a cell image including 9 views, and the like.

In addition, the displacement platform can also receive and drive the microscope lens and the camera to move to different view position coordinates through the corresponding motion module according to the full-view scanning control instruction sent by the processor; a plurality of cell images capable of covering all the fields of view are acquired by a microscope lens and a camera. The processor then splices the plurality of cell images to obtain a cell image which comprises 30 fields of view and covers all fields of view, so that full-field scanning of cells in the cell culture vessel is realized.

In this specification, each embodiment is described in a progressive manner, and identical and similar parts of each embodiment are all referred to each other, and each embodiment mainly describes differences from other embodiments. In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the embodiments of the present specification. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.

The foregoing is merely an example of an embodiment of the present disclosure and is not intended to limit the embodiment of the present disclosure. Various modifications and variations of the illustrative embodiments will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, or the like, which is within the spirit and principles of the embodiments of the present specification, should be included in the scope of the claims of the embodiments of the present specification.

Claims (10)

1. A visual inspection apparatus for cell growth status, comprising at least: a cell culture vessel, a light source, and a base structure; wherein the cell culture vessel is arranged at the upper position of the base structure; the cell culture vessel is a transparent cell culture vessel; the light source is arranged at the upper position of the cell culture vessel; the cell culture vessel is used for containing cells to be cultured;

The base structure at least comprises a displacement table and a microscopic imaging component; wherein the microscopic imaging component is connected with the displacement table; an observation window is also arranged at the position where the base structure is connected with the cell culture vessel; the microscopic imaging component is arranged at the lower position of the observation window;

the displacement table at least comprises an optical axis movement module; the base structure is also connected with the processor; the optical axis movement module is used for driving the microscopic imaging component to move along the optical axis direction;

The processor is connected with the displacement table and the microscopic imaging component; the processor is used for sending corresponding control instructions to the displacement table and the microscopic imaging assembly to realize automatic focusing of the visual cell growth state detection equipment.

2. The visual inspection apparatus for a cell growth state according to claim 1, further comprising: a light source controller; wherein, the light source controller is respectively connected with the light source and the processor.

3. The visual inspection apparatus for cell growth status according to claim 2, wherein a light sensor is provided at a position adjacent to the microscopic imaging assembly; the light sensor is connected with the processor.

4. A visual inspection apparatus for cell growth status according to claim 3, further comprising a range finder positioned adjacent to the microscopic imaging assembly; the distance meter is connected with the processor.

5. The visual inspection apparatus for cell growth status according to claim 4, wherein the displacement table is provided with a connection plate; the microscopic imaging component is arranged at the upper position of the connecting plate; and a telescopic component is further arranged between the microscopic imaging component and the connecting plate.

6. The visual inspection apparatus for cell growth status according to claim 3, further comprising a light source holder; the light source is arranged at the upper position of the cell culture vessel through the light source support.

7. The visual inspection apparatus for cell growth status according to claim 6, wherein the light source holder comprises at least a telescopic rod.

8. The visual inspection apparatus for cell growth status according to claim 7, wherein the light source holder further comprises a telescopic control motor; the telescopic control motor is electrically connected with the telescopic rod and the light source controller respectively.

9. The visual inspection apparatus for cell growth status according to claim 1, wherein the optical axis movement module comprises at least: an optical axis motor, a positioning shaft and an output shaft connecting block; wherein, the go out axle connecting block with microscopic imaging subassembly links to each other.

10. The visual inspection apparatus for cell growth status according to claim 1, wherein said microscopic imaging assembly comprises at least: a micro lens and a camera; wherein, the microscope lens and the camera are connected through threads.