CN220786681U - Kit for detecting tumor exosomes - Google Patents

Abstract

The utility model discloses a kit for detecting tumor exosomes, and relates to the technical field of exosome extraction. The kit comprises an upper box body and a cover body arranged on the upper surface of the upper box body, wherein the cover body comprises an upper cover and a lower cover, the lower cover can rotate on the lower end face of the upper cover, material taking openings are formed in the upper surfaces of the upper cover and the lower cover, and a handle is fixedly connected to the upper surface of the lower cover. According to the device, two layers of cover bodies capable of rotating relatively are arranged, and the upper surfaces of the cover bodies are provided with slotted holes with the same size, so that the internal reagent can be taken without opening the cover plate, all the reagents are prevented from being exposed to the outside, and the heat preservation effect is enhanced; the lifting mechanism is arranged, so that a single reagent bottle can be ejected out of the inside of the kit body to be taken, and a hand does not need to be stretched into the inside of the kit body, so that the kit is very convenient and fast; the movable baffle is arranged, so that the rest reagents in the interior can be isolated from the outside when the reagents are taken, the heat preservation effect is further enhanced, and the structure is simple.

Description

Kit for detecting tumor exosomes

Technical Field

The utility model relates to the technical field of exosome extraction, in particular to a kit for detecting tumor exosomes.

Background

Exosomes refer to small vesicles (30-150 nm) containing complex RNAs and proteins, which nowadays refer specifically to disc vesicles with diameters of 40-100nm, and various cells can secrete exosomes under normal and pathological conditions, mainly derived from multivesicles formed by the invagination of intracellular lysosome particles, and released into extracellular matrix after fusion of the outer membrane of the multivesicles with cell membranes. The separation method for exosomes mainly includes an ultracentrifugation method, a reagent method and a magnetic bead method. Among them, the ultracentrifugation method is the most classical exosome separation method, but the ultracentrifuge used is expensive and complicated in operation, and compared with the ultracentrifugation method, the reagent method and the magnetic bead method are easy to operate, and thus are widely welcomed. However, the reagent method and the magnetic bead method have certain problems in operation, and it is necessary to incubate the reagent or the magnetic beads with the sample for a certain period of time, and in order to avoid degradation or denaturation of exosomes and their contents, it is generally required to maintain a low temperature condition during incubation.

In the sample incubation process, reagents have a sinking trend under the action of gravity, so that the density of the reagents at different positions in the tube is inconsistent, the density of the lower layer is high, the density of the upper layer is low, and the final exosome separation effect is influenced. If a manual intermittent mixing method is adopted, the repeated opening of the refrigerator can influence the preservation of other reagents or objects in the refrigerator, and the long-term effect on the performance of the refrigerator can be caused.

The utility model relates to an exosome extraction kit, which comprises an exosome, an inner frame, and an outer box, wherein the exosome consists of a box body, a box cover and a box buckle, the box body is of a box-shaped structure with an open upper end and a cavity inside, the box cover is covered at the open upper end of the box body, one side of the box cover is hinged with one side of the open upper end of the box body, the other side of the open upper end of the box body is fixedly provided with the box buckle, the other side of the box cover is buckled and connected with the box body through the box buckle, the box body and the box cover are both made of polyethylene plastic plates, the interiors of the box body and the box cover are respectively provided with a polyvinyl alcohol cold storage agent layer injected in advance, the inner frame is arranged and distributed on the inner frame, the left side and the right side of the inner frame are fixedly connected with rotary shafts, the rotary shafts are of an L-shaped structure, and the left side and the upper edge of the box body are respectively provided with grooves; the utility model provides low temperature conditions for incubation, does not affect the preservation of other reagents or articles, does not affect a refrigerator, and can reduce the deposition of the reagents or magnetic beads so as not to affect the separation efficiency of exosomes; but this scheme still needs to open whole lid when the use of taking in practice, exposes whole reagent to the external world easily to reduce the heat preservation effect to need stretch into the box body with the hand and take during the use, it is comparatively inconvenient.

For this purpose, a kit for detecting tumor exosomes is proposed.

Disclosure of Invention

The utility model aims to provide a kit for detecting tumor exosomes, which can solve the problems of inconvenient taking and easiness in exposing more reagents to the outside in the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the kit for detecting the tumor exosome comprises an upper box body and a cover body arranged on the upper surface of the upper box body, wherein the cover body comprises an upper cover and a lower cover, the lower cover can rotate on the lower end surface of the upper cover, material taking openings are formed in the upper surfaces of the upper cover and the lower cover, a handle is fixedly connected to the upper surface of the lower cover, an arc-shaped groove is formed in the upper surface of the upper cover, and the handle penetrates through the arc-shaped groove; when the surface of the lower cover covers the surface of the material taking opening of the upper cover, the handle is positioned at the first limit position of the arc-shaped groove; when the material taking opening of the lower cover is overlapped with the material taking opening of the upper cover, the handle is positioned at the second limit position of the arc-shaped groove; the inside of going up the box body is provided with rotatable carousel, the surface of carousel is connected with a plurality of groups and places the mechanism, and the reagent bottle is placed in the placing end of placing the mechanism, the carousel drive the reagent bottle is stopped in the corresponding position of getting the material mouth.

As a further optimization scheme of the utility model, the upper box body is a hollow plate body, the plate body is made of polypropylene, and the hollow plate body is internally filled with a polyvinyl alcohol efficient cold storage agent.

As a further optimization scheme of the utility model, the lower end of the upper box body is provided with the lower box body, the interior of the lower box body is fixedly connected with a first motor, a driving shaft of the first motor penetrates into the upper box body and is connected with the turntable, and the first motor drives the turntable to rotate.

As a further optimization scheme of the utility model, the surface of the bottom plate of the upper box body is connected with a support column, the upper end surface of the support column is contacted with the lower end surface of the turntable, and the driving shaft of the first motor penetrates through the support column.

As a further optimization scheme of the utility model, the placing mechanism comprises a supporting plate and a shell, one end of the supporting plate is hinged with the surface of the turntable, the other end of the supporting plate is hinged with the surface of the shell, and the reagent bottle is placed in the shell.

As a further optimization scheme of the utility model, a lifting mechanism is arranged in the upper box body, and the moving end of the lifting mechanism drives the shell to rotate around the joint of the supporting plate and the turntable to the position of the material taking opening.

As a further optimization scheme of the utility model, the lifting mechanism comprises a second motor arranged in the upper box body, a screw is arranged at the output end of the second motor, a vertical sliding groove is further arranged in the upper box body, a sliding block is connected with the side surface of the screw in a threaded manner, a connecting block is arranged on the side surface of the sliding block, one end of the connecting block is inserted into the sliding groove and matched with the sliding groove, a jacking block is fixedly connected with the side surface of the sliding block, and the jacking block is positioned under the shell.

As a further optimization scheme of the utility model, a baffle plate capable of moving up and down is arranged in the upper box body, the heights of two side plates of the baffle plate are the same as the height of the upper box body, the baffle plate is U-shaped, the spacing between the positions of the openings is larger than that between the positions of the closed openings, and when the baffle plate is positioned at the lowest point, a placement mechanism corresponding to the material taking opening is positioned at the middle position of the baffle plate; the outer side edges of the two side plates are tightly contacted with the inner side surface of the upper box body, the turntable is provided with a plurality of square grooves, the section width of each square groove is the same as that of the two side plates of the baffle, and the baffle can be matched in the square grooves to move up and down.

As a further optimization scheme of the utility model, the surfaces of the upper cover and the lower cover are provided with inverted U-shaped grooves, the cross section of the baffle is similar to that of an inverted U, the cross section and the size of the inverted U-shaped grooves are the same as those of the baffle, the inverted U-shaped grooves of the upper cover contain the arc-shaped grooves and the material taking openings in the U-shaped parts, and the inverted U-shaped grooves of the lower cover do not contain handles and the material taking openings; when the baffle is located at the highest point, the baffle penetrates through the inverted U-shaped grooves on the surfaces of the upper cover and the lower cover, and the lowest point of the baffle is higher than the highest point of the placement mechanism.

As a further optimization scheme of the utility model, a through groove is formed in the surface of the lower cover, a rotating piece is fixedly connected to the surface of the baffle, a clamping rod is connected to the rotating end of the rotating piece, a protruding column is arranged on the surface of the upper cover, and when the clamping rod moves to the highest position along with the baffle, the rotating piece can be matched with the protruding column to fix the baffle.

Compared with the prior art, the utility model has the beneficial effects that:

according to the heat preservation device, the two layers of cover bodies capable of rotating relatively are arranged, the slotted holes with the same size are formed in the upper surface of the cover body, so that the internal reagent can be taken without opening the cover plate, all the reagents are prevented from being exposed to the outside, and the heat preservation effect is enhanced;

according to the reagent bottle lifting device, the lifting mechanism is arranged, so that a single reagent bottle can be ejected out of the box body to be taken, hands do not need to extend into the box body, and the reagent bottle lifting device is very convenient and fast;

this application can be when taking reagent through setting up mobilizable baffle, keeps apart inside other reagent and external world, further strengthens heat preservation effect, simple structure.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is an overall structural view of the present utility model;

FIG. 2 is a full cross-sectional view of the present utility model;

FIG. 3 is a schematic view of the internal structure of the present utility model;

FIG. 4 is a schematic view of a lifting mechanism according to the present utility model;

FIG. 5 is a schematic view of a top plate structure according to the present utility model;

FIG. 6 is a schematic view of a lower plate structure of the present utility model;

FIG. 7 is a second state diagram of a full cross-sectional view of the present utility model;

fig. 8 is a partial enlarged view at a of fig. 7.

Reference numerals illustrate:

in the figure: 1. an upper case; 2. a lower case; 3. an upper cover; 4. a lower cover; 5. a material taking port; 6. an arc-shaped groove; 7. a handle; 8. a first motor; 9. a support column; 10. a turntable; 11. a square groove; 12. a baffle; 13. a reverse U-shaped groove; 14. a clamping rod; 15. a rotating member; 16. a protruding column; 17. a connecting block; 18. a support plate; 19. a second motor; 20. a housing; 21. a reagent bottle; 22. a top block; 23. a slide block; 24. a screw; 25. and a sliding groove.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

Example 1

Referring to the partial structure of fig. 1, a kit for detecting tumor exosomes comprises an upper box body 1 and a cover body arranged on the upper surface of the upper box body 1, wherein the cover body comprises an upper cover 3 and a lower cover 4, and particularly referring to the partial structures of fig. 2, 5, 6 and 8, the lower cover 4 can rotate on the lower end surface of the upper cover 3, material taking openings 5 are formed on the upper surfaces of the upper cover 3 and the lower cover 4, a handle 7 is fixedly connected on the upper surface of the lower cover 4, an arc-shaped groove 6 is formed on the upper surface of the upper cover 3, and the handle 7 penetrates through the arc-shaped groove 6; when the surface of the lower cover 4 covers the surface of the material taking opening 5 of the upper cover 3, the handle 7 is positioned at the first limit position of the arc-shaped groove 6; when the material taking opening 5 of the lower cover 4 is overlapped with the material taking opening 5 of the upper cover 3, the handle 7 is positioned at the second limit position of the arc-shaped groove 6; referring to fig. 4, the inside of going up box body 1 is provided with rotatable carousel 10, and the surface of carousel 10 is connected with a plurality of groups and places the mechanism, and the quantity of placing the mechanism is a plurality of, and a plurality of circumference form evenly distributed is in disc 10 outside surface, and reagent bottle 21 is placed in the placing end of placing the mechanism, and carousel 10 drive reagent bottle 21 is stopped in the corresponding position of taking out the mouth 5, and upper cover 3 is fixed with last box body 1, and lower cover 4 joint is between upper cover 3 and last box body 1 up end, and lower cover 4 can rotate with last box body 1 relative upper cover 3.

The upper box body 1 is a hollow plate body, the plate body is made of polypropylene, the hollow plate body is internally filled with a polyvinyl alcohol efficient cold storage agent, a low-temperature environment is created, the lower end of the upper box body 1 is provided with a lower box body 2, the lower box body 2 is fixedly connected with a motor I8, a driving shaft of the motor I8 penetrates into the upper box body 1 and is connected with a rotary table 10, the motor I8 drives the rotary table 10 to rotate, a placing mechanism driving the outer side surface rotates, the surface of a bottom plate of the upper box body 1 is connected with a supporting column 9, the upper end face of the supporting column 9 is in contact with the lower end face of the rotary table 10, and a driving shaft of the motor I8 penetrates through the supporting column 9.

In the use of this embodiment, the upper case 1 is a low-temperature thermal insulation case, and by providing an upper cover and a lower cover that can move relatively, the taking-along and taking-along can be realized, and only the reagent bottle 21 of the corresponding taking-out port 5 is exposed and taken away, and in this embodiment, the placement mechanism is any structure capable of bearing the reagent bottle 21.

Example 2

With reference to the part of the structures shown in fig. 1 to 8, further limitation is made on the basis of embodiment 1, the placement mechanism comprises a supporting plate 18 and a shell 20, one end of the supporting plate 18 is hinged to the surface of the turntable 10, the other end of the supporting plate 18 is hinged to the surface of the shell 20, the reagent bottle 21 is placed in the shell 20, the reagent bottle is prevented from falling off, a lifting mechanism is arranged in the upper box body 1, the moving end of the lifting mechanism drives the shell 20 to rotate around the joint of the supporting plate 18 and the turntable 10 to the position of the material taking opening 5, the lifting mechanism comprises a second motor 26 arranged in the upper box body 1, the output end of the second motor 26 is provided with a screw 24, a vertical chute 25 is also arranged in the upper box body 1, the side surface of the screw 24 is in threaded connection with a sliding block 23, the side surface of the sliding block 23 is provided with a connecting block 17, one end of the connecting block 17 is inserted into the chute 25 and is matched with the chute 25, rotation of the sliding block 23 is limited, the sliding block 23 can be controlled by the rotating screw 24, the sliding block 23 is moved, a top block 22 is fixedly connected with the side surface of the sliding block 23, and the top block 22 is located under the shell 20.

In the present embodiment, the housing 20 is lifted by the lifting mechanism, that is, the reagent bottle 21 in the housing 20 is sent out of the material outlet 5.

Example 3

Based on the above embodiment 2, this embodiment refers to fig. 1 to 8, and provides a kit, which further includes the following arrangement structure on the basis of the above embodiment 2: the upper box body 1 is internally provided with a baffle plate 12 capable of moving up and down, the heights of two side plates of the baffle plate 12 are the same as the height of the upper box body, the baffle plate 12 is U-shaped, the spacing between the positions of the openings is larger than that between the positions of the closed openings, and when the baffle plate 12 is positioned at the lowest point, a placement mechanism corresponding to the material taking opening 5 is positioned at the middle position of the baffle plate 12; the outer side edges of the two side plates are tightly contacted with the inner side surface of the upper box body 1, the turntable 10 is provided with a plurality of square grooves 11, the section width of each square groove 11 is the same as that of the two side plates of the baffle 12, and the baffle 12 can be matched in the square grooves 11 to move up and down.

Further, the surfaces of the upper cover 3 and the lower cover 4 are provided with inverted U-shaped grooves 13, the section shape of the baffle 12 is similar to an inverted U shape, the section shape and the size of the inverted U-shaped grooves 13 are the same as the section shape and the size of the baffle 12, the inverted U-shaped grooves 13 of the upper cover 3 contain the arc-shaped grooves 6 and the material taking openings 5 in the U shape, and the inverted U-shaped grooves 13 formed in the lower cover 4 do not contain the handles 7 and the material taking openings 5; when the baffle 12 is located at the highest point, the baffle 12 penetrates through the inverted U-shaped grooves 13 on the surfaces of the upper cover 3 and the lower cover 4, the lowest point of the baffle 12 is higher than the highest point of the placement mechanism, the through groove is formed in the surface of the lower cover 4, a rotating piece 15 is fixedly connected to the surface of the baffle 12, the rotating end of the rotating piece 15 is connected with a clamping rod 14, a protruding column 16 is arranged on the surface of the upper cover 3, and when the clamping rod 14 moves to the highest position along with the baffle 12, the rotating piece 15 can be matched with the protruding column 16 to fix the baffle 12.

Working principle: when a reagent needs to be taken out of the reagent kit, the first motor 8 is started to drive the upper circular disc 10 to rotate, the first motor 8 is stopped after the reagent needs to be taken out is rotated to the position below the material taking opening 5, the handle 14 is held by a hand to slide the baffle 12 downwards to be overlapped with the square groove 11, then the handle 7 is held to rotate to the other side along with the arc groove 6, the lower cover 4 is driven to rotate to the position overlapped with the material taking opening 5, the second motor 19 is started to drive the screw 24 to rotate, then the sliding block 23 drives the top block 22 to move upwards along the sliding groove 25, the reagent bottle 21 is pushed to the position of the material outlet 5, the second motor 19 is reversed after the reagent is taken out, the sliding block 23 is kept to be fixed, the top block 22 moves downwards along the sliding groove 25, the shell 20 is tightly attached to the top block 22 under the action of gravity, when the limit position of the hinging device is reached, the shell 20 stops moving, the second motor 19 is closed, the handle 7 is held to rotate to the initial position along with the arc groove 6, the material outlet 5 is closed, then the clamping rod 14 is held by the hand to pull the baffle 12 upwards, and the handle 14 is hung to the protruding column 16 to fix the baffle 12 after the rotating piece 15.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and not for limiting the same; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the utility model.

Claims (10)

1. The utility model provides a kit, includes box body (1) to and install the lid at box body (1) upper surface, its characterized in that, the lid includes upper cover (3) and lower cover (4), lower cover (4) can rotate in the lower terminal surface of upper cover (3), material taking out mouth (5) have all been seted up to upper cover (3) and lower cover (4) upper surface, lower cover (4) upper surface fixedly connected with handle (7), arc wall (6) have been seted up to upper cover (3) upper surface, handle (7) run through arc wall (6); when the surface of the lower cover (4) covers the surface of the material taking opening (5) of the upper cover (3), the handle (7) is positioned at a first limit position of the arc-shaped groove (6); when the material taking opening (5) of the lower cover (4) is overlapped with the material taking opening (5) of the upper cover (3), the handle (7) is positioned at the second limit position of the arc-shaped groove (6); the inside of going up box body (1) is provided with rotatable carousel (10), the surface of carousel (10) is connected with a plurality of groups and places the mechanism, and reagent bottle (21) are placed in the placing end of placing the mechanism, carousel (10) drive reagent bottle (21) stop in material taking out mouth (5) corresponding position.

2. A kit according to claim 1, wherein: the lower box body (2) is installed to the lower extreme of going up box body (1), the inside fixedly connected with motor one (8) of lower box body (2), the drive shaft of motor one (8) runs through to in going up box body (1), with carousel (10) are connected, motor one (8) drive carousel (10) rotate.

3. A kit according to claim 2, wherein: the bottom plate surface of last box body (1) is connected with support column (9), the up end of support column (9) with the lower terminal surface contact of carousel (10), the drive shaft of motor one (8) runs through support column (9).

4. A kit according to claim 1, wherein: the placing mechanism comprises a supporting plate (18) and a shell (20), one end of the supporting plate (18) is hinged to the surface of the rotary table (10), the other end of the supporting plate (18) is hinged to the surface of the shell (20), and the reagent bottle (21) is placed in the shell (20).

5. A kit according to claim 4, wherein: the lifting mechanism is arranged in the upper box body (1), and the moving end of the lifting mechanism drives the shell (20) to rotate to the position of the material taking opening (5) around the joint of the supporting plate (18) and the rotary table (10).

6. A kit according to claim 5, wherein: elevating system is including setting up in the inside motor two (26) of last box body (1), screw rod (24) are installed to motor two (26) output, still be provided with vertical spout (25) in going up box body (1), screw rod (24) side surface threaded connection has slider (23), slider (23) side-mounting has connecting block (17), connecting block (17) one end insert in spout (25), with spout (25) cooperation, the side fixedly connected with kicking block (22) of slider (23), kicking block (22) are located under casing (20).

7. A kit according to claim 6, wherein: the upper box body (1) is internally provided with a baffle plate (12) capable of moving up and down, the heights of two side plates of the baffle plate (12) are the same as the height of the upper box body, the baffle plate (12) is U-shaped, the spacing between the positions of the openings is larger than that between the positions of the closed openings, and when the baffle plate (12) is positioned at the lowest point, a placement mechanism corresponding to the material taking opening (5) is positioned at the middle position of the baffle plate (12); the outer side edges of the two side plates are tightly contacted with the inner side face of the upper box body (1), the rotary disc (10) is provided with a plurality of square grooves (11), the section width of each square groove (11) is the same as that of the two side plates of the baffle plate (12), and the baffle plate (12) can be matched in the square grooves (11) to move up and down.

8. A kit according to claim 7, wherein: the surface of the upper cover (3) and the surface of the lower cover (4) are respectively provided with an inverted U-shaped groove (13), the cross section of the baffle (12) is similar to the inverted U-shaped, the cross section and the size of the inverted U-shaped groove (13) are the same as those of the baffle (12), the inverted U-shaped groove (13) of the upper cover (3) comprises an arc-shaped groove (6) and a material taking opening (5) in the U-shaped part, and the inverted U-shaped groove (13) formed in the lower cover (4) does not comprise a handle (7) and the material taking opening (5); when the baffle (12) is positioned at the highest point, the baffle (12) penetrates through the inverted U-shaped grooves (13) on the surfaces of the upper cover (3) and the lower cover (4), and the lowest point of the baffle (12) is higher than the highest point of the placement mechanism.

9. A kit according to claim 8, wherein: the surface of lower cover (4) has seted up wears the groove, the fixed surface of baffle (12) is connected with rotating member (15), the rotating end of rotating member (15) is connected with draw-in lever (14), the surface of upper cover (3) is provided with protruding post (16), draw-in lever (14) follow when baffle (12) remove to the highest position, rotate rotating member (15) can cooperate protruding post (16) to fix baffle (12).

10. A kit for detecting a tumor exosome comprising the kit of any one of claims 1-9, wherein: the upper box body (1) is a hollow plate body, the plate body is made of polypropylene, and the hollow plate body is internally filled with a polyvinyl alcohol efficient cold storage agent.