CN220759250U - Horizontal self-stirring in-vitro protein synthesis reactor - Google Patents
Horizontal self-stirring in-vitro protein synthesis reactor Download PDFInfo
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- CN220759250U CN220759250U CN202322387904.1U CN202322387904U CN220759250U CN 220759250 U CN220759250 U CN 220759250U CN 202322387904 U CN202322387904 U CN 202322387904U CN 220759250 U CN220759250 U CN 220759250U
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- synthesis reactor
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- 238000003756 stirring Methods 0.000 title claims abstract description 57
- 230000014616 translation Effects 0.000 title claims abstract description 29
- 238000001243 protein synthesis Methods 0.000 title claims abstract description 27
- 238000000338 in vitro Methods 0.000 title claims description 16
- 238000006243 chemical reaction Methods 0.000 claims abstract description 43
- 239000000428 dust Substances 0.000 claims abstract description 38
- 230000000903 blocking effect Effects 0.000 claims abstract description 8
- 230000008093 supporting effect Effects 0.000 claims description 11
- 238000007789 sealing Methods 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 238000003786 synthesis reaction Methods 0.000 claims description 3
- 125000003003 spiro group Chemical group 0.000 claims description 2
- 239000000376 reactant Substances 0.000 abstract description 16
- 238000000034 method Methods 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000000843 powder Substances 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 239000012295 chemical reaction liquid Substances 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241001330002 Bambuseae Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/141—Feedstock
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Abstract
The utility model discloses a horizontal self-stirring external protein synthesis reactor, which relates to the technical field of protein synthesis reactors, and aims at the problems that a large amount of dust can fly out in the process of adding powder raw materials to stir a tank body device of the existing horizontal self-stirring external protein synthesis reactor, the production environment is seriously influenced, and fresh air which cannot be uniformly conveyed into the tank is not uniformly conveyed. According to the utility model, dust in the horizontal reaction tank body can be effectively prevented from drifting out through the dust blocking component, and external dust can be effectively prevented from entering the horizontal reaction tank body, so that the reactant is prevented from being polluted, fresh air is uniformly provided for organisms through the air supply component, and the efficiency of biological reaction is ensured.
Description
Technical Field
The utility model relates to the technical field of protein synthesis reactors, in particular to a horizontal self-stirring in-vitro protein synthesis reactor.
Background
Current methods of protein synthesis can be divided into two classes: the method is characterized in that the method takes exogenous target DNA as a protein synthesis template, and substances such as substrates required by protein synthesis, auxiliary factors for transcription and translation related proteins and the like are supplemented by manual control, so that the synthesis of the target protein can be realized. The in vitro translation system can express protein without plasmid construction, transformation, cell culture, cell collection and disruption, and is a relatively rapid, time-saving and convenient protein expression mode.
The prior art can refer to the Chinese patent with the publication number of CN114307940A, and discloses a tank device of a horizontal self-stirring external protein synthesis reactor, which comprises a horizontal tank body, wherein a self-stirring blade assembly is arranged on the inner wall of the horizontal tank body, and is suitable for stirring a protein synthesis reaction liquid in the horizontal tank body in the rotation process of the horizontal tank body, but the external protein synthesis reactor still has the defects when in use:
firstly, a large amount of dust flows out in the process of adding powder raw materials for stirring, the production environment is seriously affected, and external dust also enters a tank body to pollute the reaction liquid;
secondly, fresh air which cannot be uniformly conveyed into the tank can not be conveyed, so that the biological reaction efficiency is seriously affected.
Thus, a horizontal self-stirring in vitro protein synthesis reactor is now provided.
Disclosure of Invention
Aiming at the defects of the prior art, the utility model provides the horizontal self-stirring external protein synthesis reactor, dust in the horizontal reaction tank body can be effectively prevented from drifting out through the dust blocking component, external dust can be effectively prevented from entering the horizontal reaction tank body, the reactant is prevented from being polluted, fresh air is uniformly provided for organisms through the air feeding component, so that the efficiency of biological reaction is ensured, the defects of the prior art are overcome, the problems that a large amount of dust can fly out in the process of adding powder raw materials into the tank body device of the conventional horizontal self-stirring external protein synthesis reactor, the production environment is seriously influenced, the external dust can enter the tank body to pollute the reaction liquid and the fresh air which cannot be uniformly conveyed into the tank, and the biological reaction efficiency is seriously influenced are effectively solved.
In order to achieve the above purpose, the present utility model adopts the following technical scheme:
the utility model provides a horizontal from external albumen synthesis reactor of stirring, includes the fixed plate and installs the horizontal retort body on the fixed plate, the lower terminal surface four corners department of fixed plate all is fixed with the landing leg, the top of the horizontal retort body is provided with the feeding pipe, and the spiro union has the top cap on the feeding pipe, the through-hole has been seted up to one side of the horizontal retort body, the dust blocking subassembly is installed to through-hole department, the rotary drum is installed through the bearing to the opposite side of the horizontal retort body, and the rotary drum is located the outside one end of the horizontal retort body and is sealing state and be connected with actuating mechanism, the rotary drum is located the inside outer wall of the horizontal retort body and installs the promotion stirring subassembly, the inside of rotary drum is provided with the subassembly of supplying air, the unloading subassembly is installed to bottom one side of the horizontal retort body.
Through foretell scheme, can effectually prevent through the dust that hinders in the horizontal retort body that dust subassembly that sets up from wafing out, and can effectually prevent that external dust also can enter into the horizontal retort body, prevent that the reactant from being polluted, realize providing fresh air for biological even through the subassembly of supplying air that sets up to guarantee the efficiency of biological reaction.
Preferably, the dust blocking component comprises a fixing sleeve and an inner supporting ring which are in threaded connection with the through hole, a dust filtering net is arranged between the outer wall of the supporting ring and the inner wall of the fixing sleeve, and spokes are further connected between the supporting ring and the fixing sleeve.
Through foretell scheme, can effectually prevent through the dust filter screen that sets up that dust in the horizontal retort body from wafing out, and can effectually prevent that external dust also can enter into in the horizontal retort body.
Preferably, the driving mechanism comprises a servo motor arranged on the side of the upper end of the fixing plate, the output end of the servo motor and the end part of the rotary cylinder are connected with belt pulleys, and the two belt pulleys are connected through a belt.
Through foretell scheme, drive the belt pulley through servo motor and rotate, then utilize the belt to drive the rotary drum and rotate.
Preferably, the promotion stirring subassembly includes three arc stirring board, and the both ends of every arc stirring board are fixed with the connecting rod, and the one end that the arc stirring board was kept away from to the connecting rod is connected with the outer wall of revolving drum, the lateral wall of arc stirring board and the lateral wall sliding contact of horizontal retort body.
Through foretell scheme, drive connecting rod and arc stirring board rotation when rotatory section of thick bamboo rotates, utilize the arc stirring board to promote the reactant and remove and turn in the horizontal retort body, realize the stirring to the reactant.
Preferably, the air supply assemblyIncludedThe air supply pipe is inserted into the rotary cylinder, one end of the air supply pipe extends to the outside of the horizontal reaction tank body and is connected with the air pump, and air outlet holes are formed in the bottom of the air supply pipe, which is positioned in the rotary cylinder, at equal distance.
Through the scheme, the air is pumped by the air pump and is discharged from the air outlet hole at the bottom of the air supply pipe, so that fresh air is supplied to the living beings.
Preferably, the rotary cylinder is uniformly provided with exhaust holes, and the support ring is sleeved on the air supply pipe in a sliding manner.
Through the scheme, when the rotary cylinder rotates, air discharged from the air outlet hole of the air supply pipe flows through the air outlet hole to be contacted with reactants, and the supporting ring can provide supporting effect for the air supply pipe.
Preferably, the unloading subassembly is including rotating the closing plate of installation horizontal retort body bottom one side and fixing the connecting plate between two landing legs, and one side rotation of connecting plate installs electric putter, and electric putter's upper end is connected with the bottom rotation of closing plate.
Through foretell scheme, drive closing plate through electric putter shrink and rotate downwards, simultaneously, make the arc stirring board reverse rotation through servo motor's reverse drive to the protein with reaction generation discharges horizontal retort body fast.
The beneficial effects of the utility model are as follows:
1. dust in the horizontal reaction tank body can be effectively prevented from drifting out through the dust filtering net arranged, and external dust can be effectively prevented from entering the horizontal reaction tank body, so that the reactant is prevented from being polluted.
2. Air pumped by the air pump is discharged from the air outlet hole at the bottom of the air supply pipe, and the air discharged from the air outlet hole of the air supply pipe flows through the air outlet hole to be contacted with the reactant, so that fresh air is provided for biological uniformity, and the efficiency of biological reaction is ensured.
In summary, dust in the horizontal reaction tank body can be effectively prevented from drifting out through the dust blocking component, and external dust can be effectively prevented from entering the horizontal reaction tank body, so that the reactant is prevented from being polluted, fresh air is uniformly provided for organisms through the air feeding component, and the efficiency of biological reaction is ensured.
Drawings
Fig. 1 is a schematic view of a first perspective three-dimensional structure of a horizontal self-stirring in-vitro protein synthesis reactor according to the present utility model.
Fig. 2 is a schematic view of a second perspective view of a horizontal self-stirring in-vitro protein synthesis reactor according to the present utility model.
Fig. 3 is a schematic diagram of a pushing and stirring assembly of a horizontal self-stirring in-vitro protein synthesis reactor according to the present utility model.
Fig. 4 is an enlarged schematic view of the structure of a horizontal self-stirring in-vitro protein synthesis reactor at a position a in fig. 1.
In the figure: 1. a fixing plate; 2. a horizontal reaction tank body; 3. a through hole; 4. an air supply pipe; 5. a fixed sleeve; 6. a support leg; 7. a servo motor; 8. a belt pulley; 9. a sealing plate; 10. an electric push rod; 11. a connecting plate; 12. a rotary drum; 13. a connecting rod; 14. an arc stirring plate; 15. an exhaust hole; 16. a support ring; 17. a dust filtering net; 18. a feeding pipe; 19. and a top cover.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.
1, referring to fig. 1-4, a horizontal self-stirring in-vitro protein synthesis reactor comprises a fixed plate 1 and a horizontal reaction tank body 2 arranged on the fixed plate 1, supporting legs 6 are fixed at four corners of the lower end surface of the fixed plate 1, a feeding pipe 18 is arranged at the top end of the horizontal reaction tank body 2, a top cover 19 is screwed on the feeding pipe 18, a through hole 3 is formed in one side of the horizontal reaction tank body 2, a dust blocking component is arranged at the through hole 3 and comprises a fixed sleeve 5 and an inner supporting ring 16 which are screwed at the through hole 3, a dust filtering net 17 is arranged between the outer wall of the supporting ring 16 and the inner wall of the fixed sleeve 5, and spokes are further connected between the supporting ring 16 and the fixed sleeve 5;
the dust filter net 17 can effectively prevent dust in the horizontal reaction tank body 2 from drifting out, and can effectively prevent external dust from entering the horizontal reaction tank body 2;
the other side of the horizontal reaction tank body 2 is provided with a rotary drum 12 through a bearing, one end of the rotary drum 12 positioned outside the horizontal reaction tank body 2 is in a sealing state and is connected with a driving mechanism, the driving mechanism comprises a servo motor 7 arranged on the side of the upper end of a fixed plate 1, the output end of the servo motor 7 and the end part of the rotary drum 12 are both connected with belt pulleys 8, and the two belt pulleys 8 are connected through a belt;
the belt pulley 8 is driven to rotate by the servo motor 7, and then the rotary drum 12 is driven to rotate by a belt;
the outer wall of the rotary cylinder 12, which is positioned in the horizontal reaction tank body 2, is provided with a pushing and stirring assembly, the pushing and stirring assembly comprises three arc stirring plates 14, two ends of each arc stirring plate 14 are fixedly provided with connecting rods 13, one end, away from the arc stirring plates 14, of each connecting rod 13 is connected with the outer wall of the rotary cylinder 12, and the side wall of the arc stirring plate 14 is in sliding contact with the side wall of the horizontal reaction tank body 2;
when the rotary cylinder 12 rotates, the connecting rod 13 and the arc stirring plate 14 are driven to rotate, and reactants are pushed to move and turn in the horizontal reaction tank body 2 by the arc stirring plate 14, so that the reactants are stirred;
an air supply assembly is arranged in the rotary cylinder 12, and the air supply assemblyIncludedThe air supply pipe 4 is inserted into the rotary cylinder 12, one end of the air supply pipe 4 extends to the outside of the horizontal reaction tank body 2 and is connected with the air pump, and the bottom of the air supply pipe 4 in the rotary cylinder 12 is provided with air outlet holes at equal distance;
air is pumped by an air pump and discharged from an air outlet hole at the bottom of the air supply pipe 4, so that fresh air is supplied to organisms;
the rotary cylinder 12 is uniformly provided with the exhaust holes 15, the support ring 16 is sleeved on the air supply pipe 4 in a sliding manner, and when the rotary cylinder 12 rotates, air discharged from the air outlet holes of the air supply pipe 4 flows through the exhaust holes 15 to be contacted with reactants, and the support ring 16 can provide a supporting effect for the air supply pipe 4;
the blanking assembly is installed to the bottom one side of the horizontal reaction tank body 2, and the blanking assembly is including rotating the closing plate 9 of installing horizontal reaction tank body 2 bottom one side and fixing the connecting plate 11 between two landing legs 6, and the electric putter 10 is installed in the rotation of one side of connecting plate 11, and electric putter 10's upper end is connected with the bottom rotation of closing plate 9.
The sealing plate 9 is driven to rotate downwards by the contraction of the electric push rod 10, and meanwhile, the arc stirring plate 14 is reversely rotated by the reverse driving of the servo motor 7, so that protein generated by reaction is rapidly discharged out of the horizontal reaction tank body 2.
Working principle: the material is added into the horizontal reaction tank body 2 from the feeding pipe 18, the belt pulley 8 is driven to rotate through the servo motor 7, the rotary drum 12 is driven to rotate through the belt, the connecting rod 13 and the arc stirring plate 14 are driven to rotate when the rotary drum 12 rotates, reactants are pushed to move and flip in the horizontal reaction tank body 2 through the arc stirring plate 14, stirring of the reactants is achieved, dust in the horizontal reaction tank body 2 can be effectively prevented from drifting out through the dust filtering net 17, external dust can be effectively prevented from entering the horizontal reaction tank body 2, the reactants are prevented from being polluted, meanwhile, air pumped through the air pump is discharged from the air outlet hole at the bottom of the air supply pipe 4, the air discharged from the air outlet hole of the air supply pipe 4 is contacted with the reactants through the air outlet hole 15, fresh air is provided for the organisms, the efficiency of biological reaction is guaranteed, the sealing plate 9 is driven to rotate downwards through shrinkage of the electric push rod 10, and meanwhile, the arc stirring plate 14 is driven to reverse through reverse driving of the servo motor 7, and the protein generated by the reaction is discharged out of the horizontal reaction tank body 2 rapidly.
The present utility model is not limited to the above-mentioned embodiments, and any person skilled in the art, based on the technical solution of the present utility model and the inventive concept thereof, can be replaced or changed within the scope of the present utility model.
Claims (7)
1. The utility model provides a horizontal type is from external albumen synthesis reactor of stirring, includes fixed plate (1) and installs horizontal retort body (2) on fixed plate (1), a serial communication port, the lower terminal surface four corners department of fixed plate (1) all is fixed with landing leg (6), the top of horizontal retort body (2) is provided with feeding pipe (18), and the spiro union has top cap (19) on feeding pipe (18), through-hole (3) have been seted up to one side of horizontal retort body (2), dust blocking subassembly is installed to through-hole (3) department, rotary drum (12) are installed through the bearing to the opposite side of horizontal retort body (2), and rotary drum (12) are located the outside one end of horizontal retort body (2) and are sealing state and be connected with actuating mechanism, rotary drum (12) are located the inside outer wall of horizontal retort body (2) and install promotion stirring subassembly, the inside of rotary drum (12) is provided with the air feed subassembly, the unloading subassembly is installed to one side of the bottom of horizontal retort body (2).
2. The horizontal self-stirring in-vitro protein synthesis reactor according to claim 1, wherein the dust blocking component comprises a fixed sleeve (5) and an inner support ring (16) which are in threaded connection with the through hole (3), a dust filtering net (17) is arranged between the outer wall of the support ring (16) and the inner wall of the fixed sleeve (5), and spokes are further connected between the support ring (16) and the fixed sleeve (5).
3. The horizontal self-stirring in-vitro protein synthesis reactor according to claim 1, wherein the driving mechanism comprises a servo motor (7) arranged on the side of the upper end of the fixed plate (1), the output end of the servo motor (7) and the end part of the rotary cylinder (12) are connected with belt pulleys (8), and the two belt pulleys (8) are connected through a belt.
4. The horizontal self-stirring in-vitro protein synthesis reactor according to claim 2, wherein the pushing stirring assembly comprises three arc stirring plates (14), connecting rods (13) are fixed at two ends of each arc stirring plate (14), one end of each connecting rod (13) away from the arc stirring plate (14) is connected with the outer wall of the rotary cylinder (12), and the side wall of the arc stirring plate (14) is in sliding contact with the side wall of the horizontal reaction tank (2).
5. The horizontal self-stirring in-vitro protein synthesis reactor according to claim 2, wherein the air supply assembly comprises an air supply pipe (4) inserted into the rotary drum (12), one end of the air supply pipe (4) extends to the outside of the horizontal reaction tank body (2) and is connected with an air pump, and air outlet holes are formed in the bottom of the air supply pipe (4) in the rotary drum (12) at equal distance.
6. The horizontal self-stirring in-vitro protein synthesis reactor according to claim 5, wherein the rotary cylinder (12) is uniformly provided with exhaust holes (15), and the support ring (16) is in sliding sleeve connection with the air supply pipe (4).
7. The horizontal self-stirring in-vitro protein synthesis reactor according to claim 1, wherein the blanking component comprises a sealing plate (9) rotatably installed on one side of the bottom of the horizontal reaction tank body (2) and a connecting plate (11) fixed between two supporting legs (6), an electric push rod (10) is rotatably installed on one side of the connecting plate (11), and the upper end of the electric push rod (10) is rotatably connected with the bottom of the sealing plate (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322387904.1U CN220759250U (en) | 2023-09-04 | 2023-09-04 | Horizontal self-stirring in-vitro protein synthesis reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322387904.1U CN220759250U (en) | 2023-09-04 | 2023-09-04 | Horizontal self-stirring in-vitro protein synthesis reactor |
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| Publication Number | Publication Date |
|---|---|
| CN220759250U true CN220759250U (en) | 2024-04-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322387904.1U Active CN220759250U (en) | 2023-09-04 | 2023-09-04 | Horizontal self-stirring in-vitro protein synthesis reactor |
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| Country | Link |
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| CN (1) | CN220759250U (en) |
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- 2023-09-04 CN CN202322387904.1U patent/CN220759250U/en active Active
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