CN220729585U - Calibrating device of multifunctional biological aerosol sampler - Google Patents
Calibrating device of multifunctional biological aerosol sampler Download PDFInfo
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- CN220729585U CN220729585U CN202322201014.7U CN202322201014U CN220729585U CN 220729585 U CN220729585 U CN 220729585U CN 202322201014 U CN202322201014 U CN 202322201014U CN 220729585 U CN220729585 U CN 220729585U
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- 238000004891 communication Methods 0.000 claims description 12
- 238000005259 measurement Methods 0.000 claims description 9
- 238000012360 testing method Methods 0.000 abstract description 6
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Abstract
The utility model discloses a calibrating device of a multifunctional biological aerosol sampler, which comprises a central control mechanism, an air tightness detection mechanism, a noise detection mechanism, a flow calibrating mechanism and a reference sampling mechanism. The utility model integrates multiple functions and can realize more convenient calibration of the bioaerosol sampler. Specifically, through the central control mechanism, each functional module can be controlled, and the measured pressure value, noise value, standard flow indication value and the like can be read, recorded and stored in real time. The noise value can be accurately read in real time by utilizing the noise detection module, and the calibration test requirement is met. The flow meter module can be used for accurately calibrating the flow of the measured sampler. In addition, through integrating the reference sampling mechanism, the complicated process of independently testing the standard sampler is saved, and the test is more convenient and quicker.
Description
Technical Field
The utility model relates to the technical field of calibration of biological aerosol samplers, in particular to a calibration device of a multifunctional biological aerosol sampler.
Background
The air microorganism is an important biological component of an ecological system, and the microorganism aerosol refers to a colloid system formed by microorganisms and byproducts thereof in the air, and has various composition types including bacteria, fungi, viruses, runners, endotoxin, mycotoxin and the like. Airborne microorganisms can cause environmental pollution and human diseases, and their content is an important parameter of airborne environmental pollution. The exposure evaluation of air microorganisms is more and more paid attention to, a sampling technology is the basis of the exposure evaluation of air microorganisms, the sampling technology is realized through a bioaerosol sampler, and the collection efficiency of the sampler is directly related to the accuracy of the final result and evaluation.
The accuracy and efficiency of the bioaerosol sampler as an instrument for evaluating the microorganism content and the type of the ambient air are critical to the sampling effect. The common evaluation indexes of the biological aerosol sampler include indexes such as flow, air tightness, noise, sampling efficiency and the like according to the standard GB/T39990-2021 technical condition of the biological aerosol sampler, and the calibration of each index corresponds to a calibration instrument. For convenience of use and simplicity of operation, it is necessary to develop a calibration device for a multifunctional bioaerosol sampler integrating a plurality of index calibrations and standard flow sampling, so as to realize rapid and efficient calibration of the bioaerosol sampler.
Disclosure of Invention
Aiming at the defects of the prior art, the utility model aims to provide a calibration device of a multifunctional biological aerosol sampler.
In order to achieve the above purpose, the present utility model adopts the following technical scheme:
the calibrating device of the multifunctional biological aerosol sampler comprises a central control mechanism, an air tightness detection mechanism, a noise detection mechanism, a flow calibrating mechanism and a reference sampling mechanism;
the air tightness detection mechanism comprises a first pipeline, a second pipeline and a third pipeline, wherein the first pipeline, the second pipeline and the third pipeline are communicated with each other; the first pipeline is connected with a vacuum pressure gauge, the second pipeline is connected with an air inlet of the biological aerosol sampler to be tested, the third pipeline is connected with a ball valve, and the ball valve can be opened and closed manually; the vacuum pressure gauge is in communication connection with the central control mechanism;
the noise detection mechanism comprises a noise detection module, and the noise detection module is in communication connection with the central control mechanism;
the flow calibration mechanism comprises a flow meter module which is in communication connection with the central control mechanism and is used for connecting with an air inlet of the sampler to be tested;
the reference sampling mechanism comprises a vacuum pump, a flowmeter and a sampling head, wherein the vacuum pump, the flowmeter and the sampling head are sequentially connected, and the central control mechanism is respectively connected with the vacuum pump and the flowmeter in a communication mode.
As a preferable scheme, the sampling head adopts an Andersen sampling head or an AGI-30 sampling head.
As a preferable scheme, the noise detection module adopts a high-sensitivity noise detection module, the measurement range of the noise detection module is 20-140dB/10Hz-25Hz, the uncertainty u=0.3 dB, and k=2.
As a preferable scheme, the flow meter module adopts a high-precision flow meter module, the flow rate range is 6-260L/min, the resolution is 0.01L/min, and the maximum allowable error is +/-1.0%.
As a preferable scheme, the central control mechanism adopts a singlechip.
The utility model has the beneficial effects that: the utility model integrates multiple functions and can realize more convenient calibration of the bioaerosol sampler. Specifically, through the central control mechanism, each functional module can be controlled, and the measured pressure value, noise value, standard flow indication value and the like can be read, recorded and stored in real time. The noise value can be accurately read in real time by utilizing the noise detection module, and the calibration test requirement is met. The flow meter module can be used for accurately calibrating the flow of the measured sampler. In addition, through integrating the reference sampling mechanism, the complicated process of independently testing the standard sampler is saved, and the test is more convenient and quicker.
Drawings
Fig. 1 is a schematic diagram of a calibration device according to an embodiment of the present utility model.
Detailed Description
The present utility model will be further described with reference to the accompanying drawings, and it should be noted that, while the present embodiment provides a detailed implementation and a specific operation process on the premise of the present technical solution, the protection scope of the present utility model is not limited to the present embodiment.
The embodiment provides a calibration device of a multifunctional biological aerosol sampler, which is shown in fig. 1 and comprises a central control mechanism 1, an air tightness detection mechanism 2, a noise detection mechanism 3, a flow calibration mechanism 4 and a reference sampling mechanism 5;
the air tightness detection mechanism 2 comprises a first pipeline, a second pipeline and a third pipeline, wherein the first pipeline, the second pipeline and the third pipeline are communicated with each other; the first pipeline is connected with a vacuum pressure gauge, the second pipeline is connected with an air inlet of the biological aerosol sampler to be tested, the third pipeline is connected with a ball valve, and the ball valve can be opened and closed manually; the vacuum pressure gauge is in communication connection with the central control mechanism. The central control mechanism can read and record the measured value of the vacuum pressure gauge on line and display the measured value in real time through the display screen.
The noise detection mechanism 3 comprises a noise detection module 31, and the noise detection module 31 is in communication connection with the central control mechanism 1. The central control mechanism can read and store the noise value detected by the noise detection module 31 on line and display the noise value in real time through a display screen. Specifically, in the present embodiment, the measurement range of the noise detection module is 20-140dB/10Hz-25Hz, and the uncertainty u=0.3 dB (k=2).
The flow calibration mechanism 4 comprises a flow meter module 41, wherein the flow meter module 41 is in communication connection with the central control mechanism and is used for connecting with an air inlet of the sampler to be tested. The central control mechanism can read and record the readings of the flowmeter module on line and display the readings in real time through the display screen. Specifically, in this embodiment, the flow rate range of the flow meter module is 6 to 260L/min, the resolution is 0.01L/min, and the maximum allowable error is ±1.0%.
The reference sampling mechanism 5 comprises a vacuum pump 51, a flowmeter 52 and a sampling head 53 (an Andersen/AGI-30 sampling head), wherein the vacuum pump, the flowmeter and the sampling head are sequentially connected, and the central control mechanism is respectively connected with the vacuum pump and the flowmeter in a communication way. The central control mechanism can read and record the measured value of the flowmeter on line, and adjust the power of the vacuum pump according to the measured value of the flowmeter so that the sampling flow reaches the target flow value. Specifically, when the anderson sampling head is used, the sampling flow rate may be set to 28.3L/min, and when the AGI-30 sampling head is used, the sampling flow rate may be set to 12.5L/min.
In this embodiment, the central control mechanism uses a single-chip microcomputer.
The working principle of the calibrating device is as follows:
when the air tightness detection is carried out, an air inlet of the biological aerosol sampler to be detected is connected to a second pipeline of the air tightness detection mechanism, the biological aerosol sampler to be detected is started, an air pump of the biological aerosol sampler starts to pump air, and air enters the biological aerosol sampler to be detected through a ball valve, a third pipeline, the second pipeline and the air inlet in sequence; then slowly closing the ball valve, closing the power supply of the biological aerosol sampler to be detected when the vacuum pressure measured by the vacuum pressure meter rises above 5kpa, simultaneously completely closing the ball valve and rapidly clamping the inlet pipe of the air suction pump of the biological aerosol sampler to be detected, recording the vacuum pressure value measured by the vacuum pressure meter after 1min, and ensuring that the pressure change in the biological aerosol sampler to be detected is not more than 0.15kpa within 1 min.
When noise detection is performed, the ambient (background) noise should be below 50dB (a). After the measured biological aerosol sampler is provided with the sampling medium, the measured biological aerosol sampler is started and set to be the maximum sampling flow. After the biological aerosol sampler works stably, the calibrating device is placed on the distances of the center positions of the front face, the rear face, the left face and the right face of the biological aerosol sampler to be measured, the center position is vertical to the distance by 1m, the noise value measured by the noise detecting module is obtained by the central control mechanism, and the maximum noise value is taken as the detection result of the working noise of the biological aerosol sampler to be measured. The operating noise of the bioaerosol sampler should be no greater than 62dB.
When the flow calibration is carried out, after the measured biological aerosol sampler is provided with the sampling medium, the measured biological aerosol sampler is started, and the sampling flow is set. After the measured biological aerosol sampler works stably, an air inlet of the measured biological aerosol sampler is connected with a flowmeter module of the calibrating device, the flow value measured by the flowmeter module is recorded, the measurement is repeated for 3 times, and the sampling flow indication error of the measured biological aerosol sampler is not more than +/-5%.
It should be noted that, the error of the sampling flow indication value can be calculated according to the following formula:
wherein:
Δq—sample flow error,%;
q v -a sampler flow indication or a sampler nominal sampling flow value, L/min;
-the arithmetic mean of 3 readings of the flow meter module of the calibration device, L/min.
The flow repeatability of the measured bioaerosol sampler should be no more than 2%. Specifically, the flow rate was repeatedly measured 10 times using the flow meter module, and the flow rate repeatability was calculated as follows:
wherein:
S γ -sample flow repeatability of the sampler;
q i -calibrating the i-th measured flow measurement of the device, L/min;
-means of the n flow measurements measured by the calibration device, L/min;
n-number of flow measurements.
The flow stability of the bioaerosol sampler to be measured should not exceed 5%, in particular, the flow q is measured using the flow meter module of the calibration device 0 And starting timing, reading for 5min every lmin, and reading 6 values, wherein the maximum value is recorded as q max And the smallest value is denoted as q min Flow stability was calculated as follows:
wherein:
delta-sample flow stability;
q 0 -initial standard flow value, L/min;
q max -maximum flow measurement, L/min;
q min -flow minimum measurement, L/min.
When the relative survival rate of microorganisms is tested by using the reference sampling mechanism, the tested biological aerosol sampler and the calibration device are placed into the experiment cabin together, biological aerosol is generated in the cabin, and the tested biological aerosol sampler and the vacuum pump of the reference sampling mechanism are started to collect the biological aerosol in the cabin together. In the reference sampling mechanism, the biological aerosol enters the sampling head through the vacuum pump, and the flowmeter is used for detecting the flow of the biological aerosol. And after the sampling is finished, respectively culturing and counting samples acquired by the measured biological aerosol sampler and the reference sampling mechanism by using a plate culture counting method. According to the measurement method and the requirements in the technical condition of a standard GB/T39990-2021 particle biological aerosol sampler, the relative microorganism survival rate of the detected biological aerosol sampler is the ratio of the microorganism survival rate of the detected biological aerosol sampler to the microorganism survival rate of a reference standard sampler multiplied by 100%, and the result is not less than 70%.
Various modifications and variations of the present utility model will be apparent to those skilled in the art in light of the foregoing teachings and are intended to be included within the scope of the following claims.
Claims (5)
1. The calibrating device of the multifunctional biological aerosol sampler is characterized by comprising a central control mechanism, an air tightness detection mechanism, a noise detection mechanism, a flow calibrating mechanism and a reference sampling mechanism;
the air tightness detection mechanism comprises a first pipeline, a second pipeline and a third pipeline, wherein the first pipeline, the second pipeline and the third pipeline are communicated with each other; the first pipeline is connected with a vacuum pressure gauge, the second pipeline is connected with an air inlet of the biological aerosol sampler to be tested, the third pipeline is connected with a ball valve, and the ball valve can be opened and closed manually; the vacuum pressure gauge is in communication connection with the central control mechanism;
the noise detection mechanism comprises a noise detection module, and the noise detection module is in communication connection with the central control mechanism;
the flow calibration mechanism comprises a flow meter module which is in communication connection with the central control mechanism and is used for connecting with an air inlet of the sampler to be tested;
the reference sampling mechanism comprises a vacuum pump, a flowmeter and a sampling head, wherein the vacuum pump, the flowmeter and the sampling head are sequentially connected, and the central control mechanism is respectively connected with the vacuum pump and the flowmeter in a communication mode.
2. The calibration device of a multifunctional bioaerosol sampler of claim 1, wherein the sampling head is an anderson sampling head or an AGI-30 sampling head.
3. The calibration device of the multifunctional bioaerosol sampler according to claim 1, wherein the noise detection module is a high-sensitivity noise detection module, the measurement range of which is 20-140dB/10Hz-25Hz, and the uncertainty u=0.3 dB, k=2.
4. The device for calibrating a multifunctional bioaerosol sampler according to claim 1, wherein the flow meter module is a high-precision flow meter module, the flow rate range is 6-260L/min, the resolution is 0.01L/min, and the maximum allowable error is +/-1.0%.
5. The calibration device of a multifunctional bioaerosol sampler of claim 1, wherein the central control mechanism employs a single-chip microcomputer.
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| CN202322201014.7U CN220729585U (en) | 2023-08-16 | 2023-08-16 | Calibrating device of multifunctional biological aerosol sampler |
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| CN202322201014.7U CN220729585U (en) | 2023-08-16 | 2023-08-16 | Calibrating device of multifunctional biological aerosol sampler |
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