CN220724200U - Cell culture feed supplement device - Google Patents
Cell culture feed supplement device Download PDFInfo
- Publication number
- CN220724200U CN220724200U CN202321893879.8U CN202321893879U CN220724200U CN 220724200 U CN220724200 U CN 220724200U CN 202321893879 U CN202321893879 U CN 202321893879U CN 220724200 U CN220724200 U CN 220724200U
- Authority
- CN
- China
- Prior art keywords
- fixedly connected
- cell culture
- rotary drum
- belt pulley
- feeding device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 22
- 239000006052 feed supplement Substances 0.000 title claims description 10
- 230000003028 elevating effect Effects 0.000 claims description 14
- 230000005540 biological transmission Effects 0.000 claims description 5
- 230000003044 adaptive effect Effects 0.000 claims description 2
- 239000007921 spray Substances 0.000 abstract description 8
- 230000004083 survival effect Effects 0.000 abstract description 5
- 230000001464 adherent effect Effects 0.000 abstract description 4
- 239000012531 culture fluid Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 238000003915 air pollution Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019577 caloric intake Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000009957 hemming Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture feeding device which comprises a base, wherein the upper end of the base is fixedly provided with hydraulic cylinders at the front side and the rear side close to the right end, the output end of the upper end of each hydraulic cylinder is fixedly connected with a lifting table, the middle part of each lifting table is rotatably provided with a rotary drum through a bearing, a feeding pipe is arranged in each rotary drum, the lower end of each rotary drum is fixedly connected with a shunt pipe at the lower side of an upper cover, the left end and the right end of each shunt pipe are fixedly connected with annular pipes, six groups of evenly distributed side spray heads are arranged on the outer sides of the annular pipes, and four groups of evenly distributed lower spray heads are arranged at the downward oblique angles of the annular pipes. According to the cell culture feeding device, the adherent cells can be washed more uniformly, so that the adherent cells fall off, meanwhile, the culture solution and the cells can be uniformly and fully mixed and contacted, the survival rate of the cells is improved, in addition, manual holding operation is not needed, and the labor capacity of staff is reduced.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a cell culture feeding device.
Background
In the process of cell culture, all substances which can enter cells and are utilized by the cells, participate in cell metabolic activity and maintain cell survival belong to nutrient substances, when the culture fluid is added, the traditional method mostly uses a suction pipe to carry out repeated suction and dripping operations, the adherence phenomenon of the cells needs to be relieved before and after the culture fluid is added, and meanwhile, a culture dish is shaken, so that the cells and the culture fluid can be uniformly mixed, the normal energy intake of the cells is ensured, but the process is complicated, the workload of staff is improved, and meanwhile, the falling risk exists when the cells are shaken by hand, and therefore, the cell culture feeding device is provided for solving the problems.
Disclosure of Invention
The utility model mainly aims to provide a cell culture material supplementing device which can effectively solve the problems in the background technology.
In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows: the utility model provides a cell culture feed supplement device, includes the base, the upper end of base is in the front and back both sides department of being close to the right-hand member equal fixed mounting has the pneumatic cylinder, the upper end output fixedly connected with elevating platform of pneumatic cylinder, the centre department of elevating platform is through the bearing rotation install the rotary drum, the internally mounted of rotary drum has the feed supplement pipe, the outer wall of rotary drum is being close to downside department fixedly connected with upper cover, the lower extreme of rotary drum is being close to downside department fixedly connected with shunt tubes of upper cover, the left and right sides fixedly connected with ring pipe of shunt tubes, be linked together between ring pipe and the shunt tubes, the outside of ring pipe is provided with six sets of evenly distributed's side shower nozzle, the decurrent oblique angle position of ring pipe is provided with four sets of evenly distributed's lower shower nozzle.
Preferably, the middle part of the upper end of the base is provided with a groove, an adaptive culture dish is placed in the groove, the outer wall of the culture dish is provided with a curled edge, and the lower end of the curled edge is attached to the upper end face of the base.
Preferably, the upper cover is matched with the culture dish.
Preferably, the front end and the rear end of the annular tube are fixedly connected with the upper cover through fixing pieces.
Preferably, the upper end of elevating platform is being close to right side centre department fixedly connected with three-phase motor, the lower extreme output of three-phase motor has fixedly cup jointed main belt pulley, the outer wall of rotary drum has fixedly cup jointed from the belt pulley in the upside department of elevating platform, be provided with driving belt between from belt pulley and the main belt pulley, from the belt pulley through driving belt and main belt pulley between transmission connection.
Preferably, the lifting platform penetrates through and is connected with the guide rod in a sliding manner at the middle position close to the left end and the right end, and the guide rod is fixedly connected with the base.
Compared with the prior art, the utility model has the following beneficial effects:
can control the switch of elevating platform drive upper cover to the culture dish through the pneumatic cylinder, provide sealed environment when the feed supplement, can avoid splashing and receive outside air pollution's risk, can rotate when spraying the culture solution through starting three-phase motor and finally can drive side shower nozzle and lower shower nozzle, and then can be more even wash away the adherence cell, make it drop, can make culture solution and cell misce bene fully contact simultaneously, the survival rate of cell has been improved, need not manual handheld operation in addition, staff's the amount of labour has been reduced, and the risk that the culture dish probably falls from the hand has been avoided.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a cell culture feeding device according to the present utility model;
FIG. 2 is a schematic view showing a partial sectional structure of a cell culture feeding device according to the present utility model;
FIG. 3 is an enlarged schematic view of the cell culture feeding device according to the present utility model at A in FIG. 2.
In the figure: 1. a base; 2. a groove; 3. a culture dish; 4. hemming; 5. a hydraulic cylinder; 6. a lifting table; 7. a bearing; 8. a rotating drum; 9. a material supplementing pipe; 10. an upper cover; 11. a shunt; 12. an annular tube; 13. a side nozzle; 14. a lower nozzle; 15. a fixing member; 16. a three-phase motor; 17. a main pulley; 18. a slave pulley; 19. a drive belt; 20. a guide rod.
Detailed Description
The utility model is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the utility model easy to understand.
As shown in fig. 1-3, a cell culture feeding device comprises a base 1, wherein the upper end of the base 1 is fixedly provided with a hydraulic cylinder 5 at the front side and the rear side close to the right end, the output end of the upper end of the hydraulic cylinder 5 is fixedly connected with a lifting table 6, the middle part of the lifting table 6 is rotatably provided with a rotary drum 8 through a bearing 7, the inside of the rotary drum 8 is provided with a feeding pipe 9, the outer wall of the rotary drum 8 is fixedly connected with an upper cover 10 close to the lower side, the lower end of the rotary drum 8 is fixedly connected with a split pipe 11 at the lower side of the upper cover 10, the left end and the right end of the split pipe 11 are fixedly connected with annular pipes 12, the annular pipes 12 are communicated with the split pipe 11, six groups of evenly distributed side spray heads 13 are arranged at the outer sides of the annular pipes 12, and four groups of evenly distributed lower spray heads 14 are arranged at downward oblique angles of the annular pipes 12;
a groove 2 is formed in the middle of the upper end of the base 1, a culture dish 3 which is matched with the groove 2 is arranged in the groove, a curled edge 4 is arranged on the outer wall of the culture dish 3, the lower end of the curled edge 4 is attached to the upper end face of the base 1, and the upper cover 10 is matched with the culture dish 3;
the culture dish 3 for cell culture is put in recess 2 after opening, can avoid culture dish 3 to drop the risk of being broken, drive elevating platform 6 through starting pneumatic cylinder 5 and descend, can drive the rotary drum 8 and upper cover 10 etc. decline through elevating platform 6 decline, stop when until upper cover 10 covers and laminate with turn-up 4 on culture dish 3, thereby make things convenient for subsequent culture solution to add the operation, can avoid splashing and receive the risk of outside air pollution, it is worth noting that this mechanism all need to disinfect and sterilize before the use, the inside of culture dish 3 is entered into to ring pipe 12 at this moment, thereby through with the feed supplement pipe 9 with pumping device link to each other can directly be in inside sterile environment's culture solution direct suction shunt tubes 11, and then through side shower nozzle 13 and lower shower nozzle 14 blowout after entering into the inside of ring pipe 12, realize the feed supplement operation, can carry out suitable washing to the inner wall of culture dish 3 through the spun nutrient solution simultaneously.
The front end and the rear end of the annular tube 12 are fixedly connected with the upper cover 10 through the fixing pieces 15, so that the structural strength of the annular tube 12 is enhanced, and deformation, fracture and other conditions are avoided.
The upper end of the lifting table 6 is fixedly connected with a three-phase motor 16 near the middle of the right side, the output end of the lower end of the three-phase motor 16 is fixedly sleeved with a main belt pulley 17, the outer wall of the rotary drum 8 is fixedly sleeved with a secondary belt pulley 18 at the upper side of the lifting table 6, a transmission belt 19 is arranged between the secondary belt pulley 18 and the main belt pulley 17, and the secondary belt pulley 18 is in transmission connection with the main belt pulley 17 through the transmission belt 19;
further, the main belt pulley 17 is driven to rotate by starting the three-phase motor 16, the driving belt 19 is driven to rotate by the main belt pulley 17, the rotary drum 8 can be driven to rotate by the rotation of the auxiliary belt pulley 18 in cooperation with the bearing 7, and the annular tube 12 can be driven to rotate finally, so that the side spray head 13 and the lower spray head 14 can rotate while spraying culture solution, further, adherent cells can be washed more uniformly, the adherent cells are enabled to fall off, meanwhile, the culture solution and the cells are uniformly and fully mixed and contacted, the survival rate of the cells is improved, manual holding operation is not needed, the labor capacity of workers is reduced, and the risk that the culture dish 3 can slide from hands is avoided.
The elevating platform 6 runs through and is connected with the guide rod 20 in a sliding manner at the middle position close to the left end and the right end, the guide rod 20 is fixedly connected with the base 1, and the fiber constraint is carried out on the elevating platform 6 through the guide rod 20, so that the stability and the level of the elevating platform 6 are ensured.
Working principle:
when in use, the culture dish 3 for cell culture is placed in the groove 2 after being opened, the risk that the culture dish 3 falls off and is broken can be avoided, the lifting table 6 is driven to descend by starting the hydraulic cylinder 5, the rotary drum 8, the upper cover 10 and the like can be driven to descend by the lifting table 6 until the upper cover 10 is covered on the culture dish 3 and is stopped when being attached to the curled edge 4, thus the subsequent culture solution adding operation is convenient, the risk of splashing and pollution by external air can be avoided, and the mechanism is notable in that disinfection and sterilization treatment are required before use, at the moment, the annular tube 12 enters the culture dish 3, the culture solution in an internal sterile environment can be directly pumped into the shunt tube 11 by connecting the feed tube 9 with the pumping device, and then is sprayed out through the side spray head 13 and the lower spray head 14 after entering the interior of the annular tube 12, realize the feed supplement operation, can carry out suitable washing away to the inner wall of culture dish 3 through spun nutrient solution simultaneously, further, drive main belt pulley 17 through starting three-phase motor 16 and rotate, make drive belt 19 drive from belt pulley 18 rotate through main belt pulley 17 rotation, can drive rotary drum 8 through from belt pulley 18 normal running fit bearing 7 and rotate, thereby finally can drive ring pipe 12 and rotate, make side shower nozzle 13 and lower shower nozzle 14 can rotate when spraying the culture solution, and then can more even wash away the adherence cell, make it drop, can make culture solution and cell misce bene fully contact simultaneously, the survival rate of cell has been improved, need not artifical handheld operation in addition, staff's amount of labour has been reduced, and the risk of the possible follow hand landing of culture dish 3 has been avoided.
The foregoing has shown and described the basic principles and main features of the present utility model and the advantages of the present utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made without departing from the spirit and scope of the utility model, which is defined in the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (6)
1. Cell culture feed supplement device, including base (1), its characterized in that: the upper end of base (1) is in the equal fixed mounting of front and back both sides department that is close to the right-hand member pneumatic cylinder (5), the upper end output fixedly connected with elevating platform (6) of pneumatic cylinder (5), the centre department of elevating platform (6) is rotated through bearing (7) and is installed rotary drum (8), internally mounted of rotary drum (8) has feed supplement pipe (9), the outer wall of rotary drum (8) is being close to downside department fixedly connected with upper cover (10), the lower extreme of rotary drum (8) is in downside department fixedly connected with shunt tubes (11) of upper cover (10), both ends fixedly connected with ring pipe (12) about shunt tubes (11), be linked together between ring pipe (12) and shunt tubes (11), the outside of ring pipe (12) is provided with six sets of evenly distributed's side shower nozzle (13), ring pipe (12) decurrent oblique angle position is provided with four sets of evenly distributed's lower shower nozzle (14).
2. A cell culture feeding device according to claim 1, wherein: the culture dish is characterized in that a groove (2) is formed in the middle of the upper end of the base (1), an adaptive culture dish (3) is placed in the groove (2), a curled edge (4) is arranged on the outer wall of the culture dish (3), and the lower end of the curled edge (4) is attached to the upper end face of the base (1).
3. A cell culture feeding device according to claim 1, wherein: the upper cover (10) is matched with the culture dish (3).
4. A cell culture feeding device according to claim 1, wherein: the front end and the rear end of the annular tube (12) are fixedly connected with the upper cover (10) through fixing pieces (15).
5. A cell culture feeding device according to claim 1, wherein: the upper end of elevating platform (6) is being close to right side centre department fixedly connected with three-phase motor (16), main belt pulley (17) have been cup jointed to the lower extreme output of three-phase motor (16), the outer wall of rotary drum (8) is fixed cup jointing from belt pulley (18) in the upside department of elevating platform (6), be provided with driving belt (19) between from belt pulley (18) and main belt pulley (17), from belt pulley (18) through driving belt (19) and main belt pulley (17) between the transmission be connected.
6. A cell culture feeding device according to claim 1, wherein: the lifting table (6) penetrates through the middle positions close to the left end and the right end and is connected with a guide rod (20) in a sliding mode, and the guide rod (20) is fixedly connected with the base (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321893879.8U CN220724200U (en) | 2023-07-18 | 2023-07-18 | Cell culture feed supplement device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321893879.8U CN220724200U (en) | 2023-07-18 | 2023-07-18 | Cell culture feed supplement device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220724200U true CN220724200U (en) | 2024-04-05 |
Family
ID=90501025
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321893879.8U Active CN220724200U (en) | 2023-07-18 | 2023-07-18 | Cell culture feed supplement device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220724200U (en) |
-
2023
- 2023-07-18 CN CN202321893879.8U patent/CN220724200U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN211587680U (en) | Breed and use disinfection brushing device | |
| CN220724200U (en) | Cell culture feed supplement device | |
| CN209420563U (en) | A kind of automatic spraying device for fruits and vegetables sprinkling irrigation | |
| CN212118655U (en) | Portable sheep hurdle epidemic prevention sterilizer | |
| CN215611482U (en) | Stirring reaction kettle for synthesizing 2, 3-difluoronitrobenzene | |
| CN206855512U (en) | A kind of vegetables clean automatically and cutter device | |
| CN215500591U (en) | Novel cultivation of chinese-medicinal material device | |
| CN213463287U (en) | Novel modern garden irrigation equipment with excellent regulation and control performance | |
| CN212944503U (en) | Belt cleaning device of trough is used to pig | |
| CN213463277U (en) | Spray type irrigation equipment for vegetable planting | |
| CN208844107U (en) | A kind of cell culture apparatus for laboratory | |
| CN212699779U (en) | Atomizing sterilizer | |
| CN212087172U (en) | Afforestation is with circulation water conservation formula irrigation equipment | |
| CN212416433U (en) | Public environment sanitation degassing unit | |
| CN210545823U (en) | Anticorrosive paint spraying device for water pump processing | |
| CN210504448U (en) | Conveyor is used in facial mask production | |
| CN221179058U (en) | Fruit tree greenhouse laxative machine | |
| CN211914731U (en) | Multifunctional medical instrument cleaning device | |
| CN212374855U (en) | Hair care cream production filling equipment | |
| CN216601397U (en) | Municipal afforestation maintenance sprinkler | |
| CN216017672U (en) | Container for sulfuric acid delinting of cotton seeds | |
| CN220493799U (en) | A spray moisturizing device for glossy ganoderma is planted | |
| CN221141697U (en) | Quick fermenting installation of domestic fungus liquid bacterial | |
| CN212733425U (en) | Fermentation cylinder that food processing used | |
| CN220003628U (en) | Disinfectant preparing machine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |