CN220685144U - Automatic sample loading device of nucleic acid isothermal amplification instrument - Google Patents
Automatic sample loading device of nucleic acid isothermal amplification instrument Download PDFInfo
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- CN220685144U CN220685144U CN202321902923.7U CN202321902923U CN220685144U CN 220685144 U CN220685144 U CN 220685144U CN 202321902923 U CN202321902923 U CN 202321902923U CN 220685144 U CN220685144 U CN 220685144U
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- nucleic acid
- sample storage
- storage box
- isothermal amplification
- loading device
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- 238000011901 isothermal amplification Methods 0.000 title claims abstract description 30
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 29
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 29
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 29
- 238000006243 chemical reaction Methods 0.000 claims description 18
- 238000009413 insulation Methods 0.000 claims description 7
- 230000003321 amplification Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses an automatic sample loading device of a nucleic acid isothermal amplification instrument, and relates to the technical field of nucleic acid isothermal amplification. The utility model comprises an upper opening sample storage box, wherein the lower end of a hydraulic rod connected with the lower side of a first hydraulic cylinder which is connected with the upper surface of the upper opening sample storage box at equal intervals is connected with the upper surface of a movable transverse plate, and the positions of pushing shafts which are connected with the lower surface of the movable transverse plate at equal intervals are in one-to-one correspondence with the positions of first through holes which are formed in the lower surface of the upper opening sample storage box at equal intervals; the connecting rod is arranged at the rear end of the hydraulic rod connected with the rear side of the second hydraulic cylinder symmetrically connected with the two sides of the upper opening sample storage box, and the push rod arranged on one side of the connecting rod, far away from the second hydraulic cylinder, penetrates through the rear side of the upper opening sample storage box. According to the utility model, the first hydraulic cylinder, the movable transverse plate, the pushing shaft and the first through hole are arranged, so that samples in a plurality of test tubes are automatically and simultaneously fed, and the second hydraulic cylinder, the connecting rod and the pushing rod are arranged, so that continuous and automatic sample feeding is facilitated.
Description
Technical Field
The utility model belongs to the technical field of isothermal amplification of nucleic acid, and particularly relates to an automatic sample loading device of a nucleic acid isothermal amplification instrument.
Background
The nucleic acid isothermal amplification technology is a nucleic acid in vitro amplification technology, the reaction process of which is always maintained at a constant temperature, and the purpose of rapid nucleic acid amplification is achieved by adding enzymes with different activities and respective specific primers, and at present, corresponding instruments are used in the nucleic acid isothermal amplification technology, such as: the nucleic acid isothermal amplification instrument mainly comprises the following operation processes: and placing the test tube filled with the sample in a placement groove on a nucleic acid isothermal amplification instrument, and then controlling the nucleic acid isothermal amplification instrument to work so as to detect the sample in the test tube.
Through searching, a portable constant-temperature amplification detector is disclosed in patent document with patent publication number of CN209923352U, and relates to the technical fields of biological science research and application. The portable isothermal amplification detector comprises a shell, a laser, a test tube rack, a detector, a heater and a processor, wherein the laser, the test tube rack, the detector, the heater and the processor are arranged in the shell; the laser, the test tube rack and the detector are sequentially arranged at intervals, and after the incident light rays emitted by the laser pass through the test tubes on the test tube rack, the transmitted light rays can be collected by the detector; the heater is arranged on the test tube rack, and the processor is electrically connected with the detector. The portable constant-temperature amplification detector can perform qualitative and quantitative detection on the detected nucleic acid sample in the solution by detecting the change of absorption light or fluorescent signals with time after the laser irradiates the solution.
However, the following drawbacks still exist in practical use:
1. in the use process of the conventional portable constant-temperature amplification detector, a worker is required to place the test tubes filled with the samples in the accommodating grooves one by one, so that a plurality of test tubes filled with the samples are not convenient to be placed in the accommodating grooves at the same time, the working steps of the worker are increased, and the sample feeding efficiency is reduced;
2. the existing portable isothermal amplification detector is inconvenient to continuously feed samples in the using process, and further influences the detection efficiency of the samples in the later period.
Therefore, the existing portable isothermal amplification detector cannot meet the requirements in practical use, so that there is an urgent need for improved technology in the market to solve the above problems.
Disclosure of Invention
The utility model aims to provide an automatic sample loading device of a nucleic acid isothermal amplification instrument, which solves the problems of low sample loading efficiency and low detection efficiency of the existing portable isothermal amplification detector by arranging a first hydraulic cylinder, a movable transverse plate, a pushing shaft, a first through hole, a second hydraulic cylinder, a connecting rod and a push rod.
In order to solve the technical problems, the utility model is realized by the following technical scheme:
the utility model relates to an automatic sample loading device of a nucleic acid isothermal amplification instrument, which comprises an upper opening sample storage box, wherein the lower end of a hydraulic rod connected with the lower side of a first hydraulic cylinder which is connected with the upper surface of the upper opening sample storage box at equal intervals is connected with the upper surface of a movable transverse plate; the rear end of a hydraulic rod connected with the rear side of a second hydraulic cylinder symmetrically connected with the two sides of the upper opening sample storage box is provided with a connecting rod, a push rod arranged on one side of the connecting rod, far away from the second hydraulic cylinder, penetrates through the rear side of the upper opening sample storage box, and a rectangular through groove is formed in the front side of the upper opening sample storage box.
Further, a first external thread protruding shaft fixedly connected with the rear end of the hydraulic rod connected with the rear side of the second hydraulic cylinder is in threaded connection with an internal thread hole groove formed in one side of the connecting rod, and a second external thread protruding shaft fixedly connected with the rear end of the push rod is in threaded connection with an internal thread hole groove formed in the other side of the connecting rod.
Further, the second through hole positions which are formed in the upper surface of the sample storage rack and are arranged in the upper opening sample storage box at equal intervals correspond to the first through hole positions one by one.
Further, a supporting transverse plate connected with the front surface of the upper opening sample storage box is positioned at the lower side of the rectangular through groove, and a vertical baffle plate is fixedly connected with the front surface of the supporting transverse plate.
Further, the anti-slip base is fixedly connected with the lower surfaces of the supporting legs symmetrically connected with the two sides of the upper opening sample storage box.
Further, the positions of the first through holes formed in the lower surface of the upper opening sample storage box at equal intervals are in one-to-one correspondence with the positions of the arranging hole grooves formed in the reaction tube rack connected to the upper surface of the base at equal intervals.
Further, the lower surface of the second heat insulation plate and the lower surface of the detector, which are sequentially arranged on the front side of the reaction tube rack from inside to outside, are connected to the upper surface of the base.
Further, the lower surface of the heater, the lower surface of the first heat insulation plate and the lower surface of the laser which are sequentially arranged on the rear side of the reaction tube rack from inside to outside are connected to the upper surface of the base.
The utility model has the following beneficial effects:
1. according to the utility model, the first hydraulic cylinder, the movable transverse plate, the pushing shaft and the first through hole are arranged, so that a plurality of test tubes can be conveniently and automatically extruded into the accommodating hole groove on the reaction tube rack at one time, the workload of sample loading in the test tubes is reduced, and the sample loading efficiency in the test tubes is accelerated.
2. According to the utility model, the second hydraulic cylinder, the connecting rod and the push rod are arranged, so that the positions of the sample storage frames can be conveniently and simultaneously adjusted, the positions of the test tubes can be conveniently and simultaneously adjusted, the continuous and simultaneous sample loading of the test tubes can be facilitated, and the detection efficiency of the nucleic acid isothermal amplification instrument on samples in the test tubes can be improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic perspective view of an automatic sample loading device of a nucleic acid isothermal amplification apparatus;
FIG. 2 is a schematic perspective view of an automatic sample loading device of a nucleic acid isothermal amplification apparatus;
FIG. 3 is a schematic diagram of the connection of the first hydraulic cylinder, the second hydraulic cylinder, the upper open magazine and the push shaft;
fig. 4 is a second schematic connection diagram of the first hydraulic cylinder, the second hydraulic cylinder, the upper open magazine and the pushing shaft.
In the drawings, the list of components represented by the various numbers is as follows:
1. an upper opening sample storage box; 11. rectangular through grooves; 12. a first through hole; 2. a movable cross plate; 21. pushing the shaft; 22. a U-shaped frame; 23. a first hydraulic cylinder; 3. a vertical baffle; 31. a supporting cross plate; 4. a base; 41. a reaction tube rack; 42. a heater; 43. a laser; 44. a first heat shield; 45. a detector; 46. a second heat shield; 5. a second hydraulic cylinder; 51. a connecting rod; 52. a first male threaded male shaft; 53. a second externally threaded male shaft; 54. a push rod; 6. a sample storage rack; 61. a second through hole; 7. supporting feet; 71. an anti-skid base.
Detailed Description
The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model.
Referring to fig. 1 to 4, the utility model discloses an automatic sample loading device of a nucleic acid isothermal amplification instrument, which comprises an upper opening sample storage box 1, wherein the lower end of a hydraulic rod connected with the lower side of a first hydraulic cylinder 23 which is connected with the lower surface of a U-shaped frame 22 connected with the upper surface of the upper opening sample storage box 1 at equal intervals is connected with the upper surface of a movable transverse plate 2, and the positions of push shafts 21 which are connected with the lower surface of the movable transverse plate 2 at equal intervals are in one-to-one correspondence with the positions of first through holes 12 which are formed in the lower surface of the upper opening sample storage box 1 at equal intervals; the rear end of a hydraulic rod connected with the rear side of a second hydraulic cylinder 5 symmetrically connected with the two sides of the upper opening sample storage box 1 is provided with a connecting rod 51, a push rod 54 arranged on one side of the connecting rod 51 far away from the second hydraulic cylinder 5 penetrates through the rear side of the upper opening sample storage box 1, and a rectangular through groove 11 is formed in the front side of the upper opening sample storage box 1.
As shown in fig. 2, a first external thread protruding shaft 52 fixedly connected with the rear end of a hydraulic rod connected with the rear side of the second hydraulic cylinder 5 is in threaded connection with an internal thread hole groove formed on one side of the connecting rod 51, and a second external thread protruding shaft 53 fixedly connected with the rear end of the push rod 54 is in threaded connection with an internal thread hole groove formed on the other side of the connecting rod 51;
specifically, rotating the push rod 54 clockwise drives the second male screw shaft 53 to rotate clockwise and gradually move away from the female screw hole groove on the connecting rod 51 until the second male screw shaft 53 is detached from the connecting rod 51, rotating the connecting rod 51 clockwise until the first male screw shaft 52 is separated from the female screw hole groove on the connecting rod 51, and detaching the second hydraulic cylinder 5, the connecting rod 51 and the push rod 54; on the contrary, the second hydraulic cylinder 5, the connecting rod 51 and the push rod 54 are connected.
As shown in fig. 3 and 4, the positions of the second through holes 61 formed on the upper surface of the sample storage rack 6 which is stored in the sample storage box 1 with the upper opening at equal intervals are in one-to-one correspondence with the positions of the first through holes 12; specifically, the test tube placed in the second through hole 61 passes through the first through hole 12 accurately, so that the feeding of the sample in the test tube is facilitated.
As shown in fig. 1, a supporting transverse plate 31 connected with the front surface of the upper opening sample storage box 1 is positioned at the lower side of the rectangular through groove 11, and a vertical baffle plate 3 is fixedly connected with the front surface of the supporting transverse plate 31;
specifically, the rack 6 discharged from the inside of the upper opening magazine 1 is moved onto the support rails 31, and the vertical barrier 3 defines the position of the rack 6 on the support rails 31, preventing the rack 6 on the support rails 31 from falling.
As shown in fig. 4, the lower surfaces of the supporting legs 7 symmetrically connected with the two sides of the upper opening sample storage box 1 are fixedly connected with anti-skid bases 71; specifically, the support legs 7 allow a moving distance between the lower surface of the upper open magazine 1 and the ground.
As shown in fig. 1 and 4, the positions of the first through holes 12 formed on the lower surface of the upper open sample storage box 1 at equal intervals are in one-to-one correspondence with the positions of the hole placement grooves formed on the reaction tube rack 41 connected on the upper surface of the base 4 at equal intervals; specifically, the test tube penetrating the first through hole 12 is allowed to enter the inside of the placement hole groove on the reaction tube rack 41 without fail.
Wherein as shown in FIG. 2, the lower surface of the second heat insulation plate 46 and the lower surface of the detector 45 which are sequentially arranged from inside to outside at the front side of the reaction tube rack 41 are connected to the upper surface of the base 4; specifically, the second heat insulation plate 46 is used for placing the detector 45 in supporting contact with the heated reaction tube rack 41, so that the detector 45 is prevented from being damaged by heating;
the lower surface of the heater 42, the lower surface of the first heat insulation plate 44 and the lower surface of the laser 43 which are sequentially arranged on the rear side of the reaction tube rack 41 from inside to outside are connected to the upper surface of the base 4; specifically, the first heat shield 44 prevents the laser 43 from directly contacting the heater 42, and thus the laser 43 is damaged by heat;
specifically, the automatic sample loading device of the nucleic acid isothermal amplification instrument comprises the following steps: starting the first hydraulic cylinder 23, extending a hydraulic rod connected to the first hydraulic cylinder 23, moving the movable transverse plate 2 downwards, and moving the pushing shaft 21 downwards to squeeze the test tube in the second through hole 61 into the accommodating hole groove in the reaction tube rack 41 until the test tube completely leaves the first through hole 12, wherein the height of a gap between the upper opening sample storage box 1 and the reaction tube rack 41 is larger than the height of the test tube; when the sample in the next batch of test tubes needs to be fed, firstly, the test tubes in the reaction tube rack 41 are taken out by hands, the second hydraulic cylinder 5 is started, the hydraulic rod on the second hydraulic cylinder 5 is contracted, the connecting rod 51 drives the push rod 54 to push the sample storage rack 6 to move towards the direction close to the rectangular through groove 11 until the next sample storage rack 6 moves to the lower side of the push shaft 21, the first sample storage rack 6 passes through the rectangular through groove 11 and moves to the upper side of the supporting transverse plate 31, the first hydraulic cylinder 23 is started, and the test tubes on the next sample storage rack 6 are extruded into the accommodating hole groove of the reaction tube rack 41.
The foregoing is only a preferred embodiment of the present utility model, and the present utility model is not limited thereto, and any modification, equivalent replacement, and improvement of some of the technical features described in the foregoing embodiments are all within the scope of the present utility model.
Claims (8)
1. Automatic sample loading device of nucleic acid isothermal amplification instrument, including upper shed sample storage box (1), its characterized in that: the lower ends of hydraulic rods connected with the lower sides of first hydraulic cylinders (23) which are connected with the upper surface of the upper opening sample storage box (1) at equal intervals are connected with the upper surface of the movable transverse plate (2), and the positions of pushing shafts (21) which are connected with the lower surface of the movable transverse plate (2) at equal intervals are in one-to-one correspondence with the positions of first through holes (12) which are formed in the lower surface of the upper opening sample storage box (1) at equal intervals;
the utility model discloses a sample box, including upper shed sample box (1), second pneumatic cylinder (5) rear end that upper shed sample box (1) both sides symmetry was connected is provided with connecting rod (51) at the hydraulic rod rear end that second pneumatic cylinder (5) rear side was connected, push rod (54) that second pneumatic cylinder (5) one side was kept away from to connecting rod (51) set up runs through upper shed sample box (1) rear side, rectangular logical groove (11) have been seted up to upper shed sample box (1) front side.
2. The automatic loading device of a nucleic acid isothermal amplification instrument according to claim 1, wherein: the first external thread protruding shaft (52) fixedly connected with the rear end of the hydraulic rod connected with the rear side of the second hydraulic cylinder (5) is in threaded connection with the internal thread hole groove formed in one side of the connecting rod (51), and the second external thread protruding shaft (53) fixedly connected with the rear end of the push rod (54) is in threaded connection with the internal thread hole groove formed in the other side of the connecting rod (51).
3. The automatic loading device of a nucleic acid isothermal amplification instrument according to claim 1, wherein: the positions of the second through holes (61) which are formed in the upper surface of the sample storage rack (6) and are arranged at equal intervals in the upper opening sample storage box (1) correspond to the positions of the first through holes (12) one by one.
4. The automatic loading device for a nucleic acid isothermal amplification apparatus according to claim 3, wherein: the front surface of the upper opening sample storage box (1) is connected with a supporting transverse plate (31) which is positioned at the lower side of the rectangular through groove (11), and the front surface of the supporting transverse plate (31) is fixedly connected with a vertical baffle plate (3).
5. The automatic loading device of a nucleic acid isothermal amplification apparatus according to claim 4, wherein: the anti-slip base (71) is fixedly connected to the lower surfaces of the supporting legs (7) symmetrically connected with the two sides of the upper opening sample storage box (1).
6. The automatic loading device of a nucleic acid isothermal amplification apparatus according to claim 5, wherein: the positions of the first through holes (12) formed in the lower surface of the upper opening sample storage box (1) at equal intervals are in one-to-one correspondence with the positions of the arranging hole grooves formed in the reaction tube rack (41) connected to the upper surface of the base (4) at equal intervals.
7. The automatic loading device of a nucleic acid isothermal amplification apparatus according to claim 6, wherein: the lower surface of a second heat insulation plate (46) and the lower surface of a detector (45) which are sequentially arranged on the front side of the reaction tube rack (41) from inside to outside are connected to the upper surface of the base (4).
8. The automatic loading device of a nucleic acid isothermal amplification apparatus according to claim 6, wherein: the lower surface of the heater (42), the lower surface of the first heat insulation plate (44) and the lower surface of the laser (43) which are sequentially arranged on the rear side of the reaction tube rack (41) from inside to outside are connected to the upper surface of the base (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321902923.7U CN220685144U (en) | 2023-07-19 | 2023-07-19 | Automatic sample loading device of nucleic acid isothermal amplification instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321902923.7U CN220685144U (en) | 2023-07-19 | 2023-07-19 | Automatic sample loading device of nucleic acid isothermal amplification instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220685144U true CN220685144U (en) | 2024-03-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321902923.7U Active CN220685144U (en) | 2023-07-19 | 2023-07-19 | Automatic sample loading device of nucleic acid isothermal amplification instrument |
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| Country | Link |
|---|---|
| CN (1) | CN220685144U (en) |
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2023
- 2023-07-19 CN CN202321902923.7U patent/CN220685144U/en active Active
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