CN220671324U - Detachable microfluidic three-way chip for detecting heavy metal lead ions in water - Google Patents
Detachable microfluidic three-way chip for detecting heavy metal lead ions in water Download PDFInfo
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- CN220671324U CN220671324U CN202321923330.9U CN202321923330U CN220671324U CN 220671324 U CN220671324 U CN 220671324U CN 202321923330 U CN202321923330 U CN 202321923330U CN 220671324 U CN220671324 U CN 220671324U
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- Prior art keywords
- glass slide
- electrode
- printed
- heavy metal
- detachable
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- 150000002500 ions Chemical class 0.000 title claims abstract description 30
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 13
- 239000011521 glass Substances 0.000 claims abstract description 33
- 238000001514 detection method Methods 0.000 claims abstract description 17
- KRHYYFGTRYWZRS-UHFFFAOYSA-N Fluorane Chemical compound F KRHYYFGTRYWZRS-UHFFFAOYSA-N 0.000 claims abstract description 8
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 13
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 claims description 7
- YOCUPQPZWBBYIX-UHFFFAOYSA-N copper nickel Chemical compound [Ni].[Cu] YOCUPQPZWBBYIX-UHFFFAOYSA-N 0.000 claims description 7
- 230000007547 defect Effects 0.000 claims description 7
- 229910052697 platinum Inorganic materials 0.000 claims description 6
- 238000009396 hybridization Methods 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 229920001940 conductive polymer Polymers 0.000 claims description 4
- 108091008104 nucleic acid aptamers Proteins 0.000 claims description 4
- 229910021607 Silver chloride Inorganic materials 0.000 claims description 3
- 229910052709 silver Inorganic materials 0.000 claims description 3
- 239000004332 silver Substances 0.000 claims description 3
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 claims description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 238000011897 real-time detection Methods 0.000 description 2
- 238000011896 sensitive detection Methods 0.000 description 2
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- UYJXRRSPUVSSMN-UHFFFAOYSA-P ammonium sulfide Chemical compound [NH4+].[NH4+].[S-2] UYJXRRSPUVSSMN-UHFFFAOYSA-P 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- XTVVROIMIGLXTD-UHFFFAOYSA-N copper(II) nitrate Chemical compound [Cu+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O XTVVROIMIGLXTD-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- RVPVRDXYQKGNMQ-UHFFFAOYSA-N lead(2+) Chemical compound [Pb+2] RVPVRDXYQKGNMQ-UHFFFAOYSA-N 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- KBJMLQFLOWQJNF-UHFFFAOYSA-N nickel(ii) nitrate Chemical compound [Ni+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O KBJMLQFLOWQJNF-UHFFFAOYSA-N 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
- 239000012279 sodium borohydride Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model discloses a detachable microfluidic three-way chip for detecting heavy metal lead ions in water, which relates to the technical field of environmental heavy metal ion detection and comprises a first glass slide and a second glass slide detachably connected to the first glass slide, wherein a rectangular detachable pool groove is etched on the first glass slide by hydrofluoric acid, a double-pass pool is printed on the first glass slide, and a reference electrode and a counter electrode are respectively printed in an electrode groove in the double-pass pool; the second glass slide is detachably connected in the tooth slot, a working motor electrolytic cell is printed on the second glass slide, and a working electrode is printed in the cell. The utility model provides a detachable microfluidic three-way chip for detecting heavy metal lead ions in water, which adopts a detachable working electrode plate, has the advantages of low cost, easy operation, repeated use and stable result, and can realize real-time online rapid detection. Adding a sample to be tested on the surface of the working electrode; the lead-out terminal is connected with the electrochemiluminescence workstation to finish detection.
Description
Technical Field
The utility model relates to the technical field of environmental heavy metal ion detection, in particular to a detachable microfluidic three-way chip for detecting heavy metal lead ions in water.
Background
Heavy metal lead ions are common environmental pollutants, and widely exist as industrial pollutants, and lead ions can influence functions of a human nervous system, a cardiovascular system, a skeletal system, a reproductive system and an immune system and easily cause diseases of gastrointestinal tracts, livers, kidneys and brains. Therefore, it is important to realize high-sensitivity detection of lead ions.
Through retrieval, a heavy metal ion detection chip disclosed in Chinese patent grant publication No. CN205941377U comprises a hydrophilic substrate, wherein the surface of the substrate is covered with a hydrophobic layer, the hydrophobic layer can prevent heavy metal ions from entering the substrate and can allow organic solvents to permeate, and a color reagent capable of carrying out color reaction with at least one heavy metal ion is adsorbed on the surface of the hydrophobic layer. In the method for detecting heavy metal ions of the microfluidic paper sheet, various heavy metal ions react with specific substances in a color development manner, and the detection sensitivity of the heavy metal ions is greatly improved through surface hydrophobization treatment.
However, the detection chip cannot realize the functions of repeated use, easy operation, stable structure and real-time online rapid detection; therefore, a detachable microfluidic three-way chip for detecting heavy metal lead ions in water is designed.
Disclosure of Invention
The utility model aims to provide a detachable microfluidic three-way chip for detecting heavy metal lead ions in water, so as to solve the problems in the background technology.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
the detachable microfluidic three-way chip comprises a first glass slide and a second glass slide detachably connected to the first glass slide, wherein a rectangular detachable pool groove is etched on the first glass slide by using hydrofluoric acid, a double-pass pool is printed on the first glass slide, and a reference electrode and a counter electrode are respectively printed in an electrode groove in the double-pass pool;
the second glass slide is detachably connected in the tooth slot, a working motor electrolytic cell is printed on the second glass slide, a working electrode is printed in the cell, and the two-way cell is communicated with the working motor electrolytic cell through a three-way cell;
three electrode leads and three electrode contacts are also printed on the first glass slide;
preparing a platinum hybridization defect-rich nickel-copper bimetallic sulfide layer and a nucleic acid aptamer chain capable of specifically recognizing lead ions on the surface of the working electrode; adding a sample to be tested on the surface of the working electrode; the lead-out terminal is connected with an electrochemical workstation to finish detection.
As a further improvement of the above, the reference electrode is made of silver/silver chloride.
As a further improvement of the above scheme, the counter electrode, the working electrode and the three-way Chi Junyou graphite powder are made.
As a further improvement of the above, the electrode leads and the electrode contacts are made of a conductive polymer.
Compared with the prior art, the utility model has the beneficial effects that:
1. the utility model provides a detachable microfluidic three-way chip for detecting heavy metal lead ions in water, which adopts a detachable working electrode plate, has the advantages of low cost, easy operation, repeated use and stable result, and can realize real-time online rapid detection. Adding a sample to be tested on the surface of the working electrode; the lead-out terminal is connected with the electrochemiluminescence workstation to finish detection.
2. The utility model designs the microfluidic chip which has high flux, low cost, convenience and quickness and can realize real-time detection, and realizes sensitive detection of lead ions.
Drawings
FIG. 1 is a schematic view of a first embodiment of a slide according to the present utility model.
FIG. 2 is a schematic view of a second embodiment of the slide according to the present utility model.
Fig. 3 is a schematic overall structure of the present utility model.
In the figure: 1. a first glass slide; 2. a second glass slide; 11. a tank; 12. a double-pass pool; 13. a reference electrode; 14. a counter electrode; 15. an electrode lead; 16. an electrode contact; 17. a three-way pool; 21. a working electrode.
Detailed Description
The technical scheme of the patent is further described in detail below with reference to the specific embodiments.
1-3, a detachable microfluidic three-way chip for detecting heavy metal lead ions in water comprises a first glass slide 1 and a second glass slide 2 detachably connected to the first glass slide 1, wherein a rectangular detachable pool groove 11 is etched on the first glass slide 1 by using hydrofluoric acid, a double-pass pool 12 is printed on the first glass slide, and a reference electrode 13 made of silver/silver chloride and a counter electrode 14 made of graphite powder are respectively printed in an electrode groove in the double-pass pool 12;
the second glass slide 2 is detachably connected in the tooth slot, a working motor electrolytic cell is printed on the second glass slide 2, a working electrode 21 made of graphite powder is printed in the cell, and the double-pass cell 12 is communicated with the working motor electrolytic cell through a three-pass cell 17;
wherein, three electrode leads 15 made of conductive polymers and three electrode contacts 16 made of conductive polymers are also printed on the first glass slide 1;
wherein, a platinum hybridization defect-rich nickel-copper bimetallic sulfide layer and a nucleic acid aptamer chain capable of specifically recognizing lead ions are prepared on the surface of the working electrode 21; adding a sample to be tested on the surface of the working electrode 21; the lead-out terminal is connected with an electrochemical workstation to finish detection.
The preparation method of the platinum hybridization defect-rich nickel-copper bimetallic sulfide comprises the following steps:
adding raw materials of nickel nitrate, copper nitrate, ammonium sulfide and thiourea into a reaction kettle according to the mass ratio of 1:1:0.5:0.3, and reacting at 160 ℃ for 72 hours to obtain a defect-rich nickel-copper bimetallic sulfide V-NiCuSx; and (3) placing chloroplatinic acid, sodium borohydride, sodium citrate and V-NiCuSx into a three-neck flask according to the mass ratio of 1:0.5:0.2:1, and refluxing at high temperature for 24 hours to obtain the platinum hybridization defect-rich nickel-copper bimetallic sulfide Pt@V-NiCuSx.
The working principle of the utility model is as follows: a rectangular detachable well 11 is etched on the first slide 1, and a double-pass well 12, electrode leads 15, electrode contacts 16, and the like are printed. While the working electrode 21 pool and corresponding leads are printed on the other removable slide 2. And preparing the detachable microfluidic three-way detection chip. Then sequentially modifying platinum hybridized defect-rich nickel-copper bimetallic sulfide on a detachable working electrode 21, wherein the nucleic acid aptamer chain can specifically identify lead ions; the lead ion detection chip of the detachable microfluidic tee joint is prepared, and a sample to be detected is added on the surface of a working electrode 21; the lead-out terminal is connected with the electrochemiluminescence workstation to finish detection. The utility model designs the microfluidic chip which has high flux, low cost, convenience and quickness and can realize real-time detection, and realizes sensitive detection of lead ions.
Claims (4)
1. The detachable microfluidic three-way chip for detecting heavy metal lead ions in water comprises a first glass slide (1) and a second glass slide (2) detachably connected to the first glass slide (1), and is characterized in that a rectangular detachable tank groove (11) is etched on the first glass slide (1) by using hydrofluoric acid, a double-pass tank (12) is printed on the first glass slide, and a reference electrode (13) and a counter electrode (14) are respectively printed in an electrode groove in the double-pass tank (12);
the second glass slide (2) is detachably connected in the tank, a working motor electrolytic tank is printed on the second glass slide (2), a working electrode (21) is printed in the tank, and the two-way tank (12) is communicated with the working motor electrolytic tank through a three-way tank (17);
three electrode leads (15) and three electrode contacts (16) are also printed on the first glass slide (1);
preparing a platinum hybridization defect-rich nickel-copper bimetallic sulfide layer and a nucleic acid aptamer chain capable of specifically recognizing lead ions on the surface of the working electrode (21); adding a sample to be tested on the surface of a working electrode (21); the lead-out terminal is connected with an electrochemical workstation to finish detection.
2. A detachable microfluidic three-way chip for detecting heavy metal lead ions in water according to claim 1, wherein the reference electrode (13) is made of silver/silver chloride.
3. The detachable microfluidic three-way chip for detecting heavy metal lead ions in water according to claim 1, wherein the counter electrode (14), the working electrode (21) and the three-way cell (17) are all made of graphite powder.
4. A detachable microfluidic three-way chip for detecting heavy metal lead ions in water according to claim 1, wherein the electrode leads (15) and the electrode contacts (16) are made of conductive polymers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321923330.9U CN220671324U (en) | 2023-07-20 | 2023-07-20 | Detachable microfluidic three-way chip for detecting heavy metal lead ions in water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321923330.9U CN220671324U (en) | 2023-07-20 | 2023-07-20 | Detachable microfluidic three-way chip for detecting heavy metal lead ions in water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220671324U true CN220671324U (en) | 2024-03-26 |
Family
ID=90329739
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321923330.9U Active CN220671324U (en) | 2023-07-20 | 2023-07-20 | Detachable microfluidic three-way chip for detecting heavy metal lead ions in water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220671324U (en) |
-
2023
- 2023-07-20 CN CN202321923330.9U patent/CN220671324U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant |