CN220665348U - Protoplast culture auxiliary device - Google Patents
Protoplast culture auxiliary device Download PDFInfo
- Publication number
- CN220665348U CN220665348U CN202322268032.7U CN202322268032U CN220665348U CN 220665348 U CN220665348 U CN 220665348U CN 202322268032 U CN202322268032 U CN 202322268032U CN 220665348 U CN220665348 U CN 220665348U
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- CN
- China
- Prior art keywords
- mounting frame
- culture
- culture tank
- auxiliary device
- annular mounting
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Links
- 210000001938 protoplast Anatomy 0.000 title claims abstract description 33
- 238000007789 sealing Methods 0.000 claims abstract description 20
- 230000000712 assembly Effects 0.000 claims abstract description 17
- 238000000429 assembly Methods 0.000 claims abstract description 17
- 239000000853 adhesive Substances 0.000 claims description 8
- 230000001070 adhesive effect Effects 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims 1
- 210000000170 cell membrane Anatomy 0.000 abstract description 7
- 210000004027 cell Anatomy 0.000 description 9
- 230000005389 magnetism Effects 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229920000297 Rayon Polymers 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present utility model provides a protoplast culture auxiliary device comprising: the culture tank comprises a culture tank body, wherein a placement assembly is arranged in the culture tank body and comprises an annular mounting frame, and a film is arranged on the inner wall of the annular mounting frame; the number of the pulling assemblies is two, the two pulling assemblies are symmetrically arranged on the surface of the annular mounting frame, the pulling assemblies comprise pulling rods, one ends of the pulling rods are connected with the mounting frame, and one ends of the mounting frame are connected with the annular mounting frame; the sealing assemblies are arranged at two ends of the culture tank body respectively. According to the protoplast culture auxiliary device provided by the utility model, the pulling rods with the mounting frames are symmetrically arranged on the surface of the annular mounting frame and are matched with the films, so that after cell membranes are cultured, operators can conveniently take out the films at the central position inside the culture tank main body.
Description
Technical Field
The utility model relates to the field of protoplast culture, in particular to a protoplast culture auxiliary device.
Background
Protoplasts are the generic term for structures within the cell wall, and also form a morphological unit of structure that constitutes a cell, where various metabolic activities in living cells occur, including cell membranes, cytoplasm, nuclei, organelles, and the like.
At present, in the culture of protoplast, can cultivate the cell diaphragm, but the patent application of publication number CN218755809U that proposes among the prior art, it is through regard film as the support, can cultivate two kinds of different cells at the surface of support both sides simultaneously, pour into first cell suspension into in the first cavity through first opening, pour into second cell suspension into in the second cavity through the second opening, the cell diaphragm that two kinds of different cell suspension cultures obtained is pasted and is located the surface of support, after the cell diaphragm ripens, need not to carry out extra processing and can directly take out the film, so as to obtain whole cell diaphragm support material, easy and simple to handle, the cell diaphragm that obtains from this need not mechanical stack can realize different functions, but the current film is installed in the inside central point of culture vessel, after cultivateing the cell diaphragm, be difficult to take out the film at the inside central point of culture vessel.
Therefore, there is a need to provide a protoplast culture assistance device that solves the above-described problems.
Disclosure of Invention
The utility model provides a protoplast culture auxiliary device, which solves the problem that the film is difficult to take out from the central position inside a culture vessel after a cell membrane is cultured after the film is arranged at the central position inside the culture vessel.
In order to solve the above technical problems, the present utility model provides a protoplast culture auxiliary device, comprising:
the culture tank comprises a culture tank body, wherein a placement assembly is arranged in the culture tank body and comprises an annular mounting frame, and a film is arranged on the inner wall of the annular mounting frame;
the number of the pulling assemblies is two, the two pulling assemblies are symmetrically arranged on the surface of the annular mounting frame, the pulling assemblies comprise pulling rods, one ends of the pulling rods are connected with the mounting frame, and one ends of the mounting frame are connected with the annular mounting frame;
the sealing assemblies are arranged at two ends of the culture tank body respectively.
Preferably, the surface of the film is connected with a positioning block, a positioning groove matched with the positioning block is formed in the surface of the annular mounting frame, and a fixing bolt is arranged in the positioning block.
Preferably, the sealing assembly comprises a sealing cover, one side of the sealing cover is connected with a first magnetic attraction ring, and the surface of the culture tank main body is provided with a second magnetic attraction ring matched with the first magnetic attraction ring.
Preferably, the surface of the culture tank main body is connected with a plurality of air outlet pipes.
Preferably, one side of the sealing cover is connected with a connecting pipe.
Preferably, the surface of culture tank main part is provided with supporting component, supporting component includes the connecting plate, the both sides on connecting plate surface all are connected with the arc mounting bracket.
Preferably, the two sides of the bottom of the connecting plate are connected with supporting frames, and the bottoms of the supporting frames are adhered with adhesive.
Compared with the related art, the protoplast culture auxiliary device provided by the utility model has the following beneficial effects:
the utility model provides a protoplast culture auxiliary device, wherein pull rods with mounting frames are symmetrically arranged on the surface of an annular mounting frame and are matched with a film for operation, so that after cell membranes are cultured, an operator can conveniently take out the film at the central position inside a culture tank main body.
Drawings
FIG. 1 is a schematic view of a first embodiment of a protoplast culture auxiliary device according to the utility model;
FIG. 2 is an enlarged schematic view of portion A shown in FIG. 1;
FIG. 3 is a schematic perspective view of the entire apparatus shown in FIG. 1;
fig. 4 is a schematic structural diagram of a second embodiment of a protoplast culture auxiliary device according to the utility model.
Reference numerals in the drawings: 1. culture tank main part, 2, place the subassembly, 21, annular mounting bracket, 22, film, 23, locating piece, 24, constant head tank, 25, gim peg, 3, pulling subassembly, 31, pulling rod, 32, mounting bracket, 4, sealing subassembly, 41, sealed lid, 42, first magnetism inhale the ring, 43, second magnetism inhale the ring, 5, outlet duct, 6, connecting pipe, 7, supporting component, 71, connecting plate, 72, arc mounting bracket, 73, support frame, 74, viscose.
Detailed Description
The utility model will be further described with reference to the drawings and embodiments.
First embodiment
Referring to fig. 1, fig. 2 and fig. 3 in combination, fig. 1 is a schematic structural diagram of a first embodiment of a protoplast culture auxiliary device according to the utility model; FIG. 2 is an enlarged schematic view of portion A shown in FIG. 1; fig. 3 is a schematic perspective view of the whole device shown in fig. 1. A protoplast culture assistance device comprising:
a culture tank main body 1, wherein a placement component 2 is arranged inside the culture tank main body 1, the placement component 2 comprises an annular mounting frame 21, and a film 22 is arranged on the inner wall of the annular mounting frame 21;
the number of the pulling assemblies 3 is two, the two pulling assemblies 3 are symmetrically arranged on the surface of the annular mounting frame 21, the pulling assemblies 3 comprise pulling rods 31, one ends of the pulling rods 31 are connected with mounting frames 32, and one ends of the mounting frames 32 are connected with the annular mounting frame 21;
the sealing assemblies 4 are two in number, and the two sealing assemblies 4 are respectively arranged at two ends of the culture tank body 1.
The surface of the film 22 is connected with a positioning block 23, a positioning groove 24 matched with the positioning block 23 is formed in the surface of the annular mounting frame 21, and a fixing bolt 25 is arranged in the positioning block 23.
The use of locating piece 23 can install the surface at annular mounting bracket 21 to film 22, installs annular blocking piece in one side of culture tank main part 1 inner wall, and annular blocking piece's use can install annular mounting bracket 21 in the inside central point of culture tank main part 1 put, and first magnetism is inhaled the ring 42 and second magnetism is inhaled the ring 43 and can be fixed sealed lid 41 at the tip of culture tank main part 1, and first magnetism is inhaled the ring 42 and second magnetism is inhaled the ring 43 and is the principle that opposite phase is inhaled.
The sealing assembly 4 comprises a sealing cover 41, a first magnetic suction ring 42 is connected to one side of the sealing cover 41, and a second magnetic suction ring 43 matched with the first magnetic suction ring 42 is arranged on the surface of the culture tank main body 1.
The surface of the culture tank main body 1 is connected with a plurality of air outlet pipes 5.
The air outlet pipe 5 plays a role of exhausting air, and the embryo can be placed on the film 22 by using the connecting pipe 6.
A connection pipe 6 is connected to one side of the sealing cover 41.
The working principle of the protoplast culture auxiliary device provided by the utility model is as follows:
when in use, when the film 22 is taken, the sealing cover 41 is firstly taken out, after the sealing cover 41 is taken out, the pulling rod 31 is pulled to drive the mounting frame 32 to move, the annular mounting frame 21 is driven to move in the culture tank main body 1 when the mounting frame 32 moves, the film 22 is driven to move towards the outer side of the culture tank main body 1 when the annular mounting frame 21 moves, after the film 22 moves to the outer side of the culture tank main body 1, the fixing bolt 25 on the surface of the positioning block 23 is taken out, and the film 22 can be taken out in the annular mounting frame 21.
Compared with the related art, the protoplast culture auxiliary device provided by the utility model has the following beneficial effects:
the utility model provides a protoplast culture auxiliary device, wherein pull rods 31 with mounting frames 32 are symmetrically arranged on the surface of an annular mounting frame 21 and matched with a film 22, so that after cell membranes are cultured, operators can conveniently take out the film 22 at the central position inside a culture tank main body 1.
Second embodiment
Referring to fig. 4 in combination, another protoplast culture assistance device is provided according to a second embodiment of the present application. The second embodiment is merely a preferred manner of the first embodiment, and implementation of the second embodiment does not affect the implementation of the first embodiment alone.
Specifically, the second embodiment of the present application provides a protoplast culture auxiliary device, which is characterized in that a protoplast culture auxiliary device, the surface of the culture tank body 1 is provided with a supporting component 7, the supporting component 7 comprises a connecting plate 71, and both sides of the surface of the connecting plate 71 are connected with arc-shaped mounting frames 72.
The two sides of the bottom of the connecting plate 71 are connected with a supporting frame 73, and the bottom of the supporting frame 73 is adhered with an adhesive 74.
The adhesive 74 may be used to secure the entire device, and the adhesive 74 is a nano-sized double sided adhesive.
The working principle of the protoplast culture auxiliary device provided by the utility model is as follows:
when in use, when the cell membrane is cultured, the whole device is fixed at a designated position through the adhesive 74 at the bottom of the supporting frame 73, and then the culture tank body 1 is placed on the surface of the arc-shaped mounting frame 72 on the surface of the connecting plate 71.
Compared with the related art, the protoplast culture auxiliary device provided by the utility model has the following beneficial effects:
the utility model provides a protoplast culture auxiliary device, which is characterized in that a connecting plate 71 with an arc-shaped mounting frame 72 and a supporting frame 73 with an adhesive 74 are arranged on the surface of a culture tank main body 1 to cooperate with each other, so that the culture tank main body 1 can be placed when cell membranes are cultured.
The foregoing description is only illustrative of the present utility model and is not intended to limit the scope of the utility model, and all equivalent structures or equivalent processes or direct or indirect application in other related technical fields are included in the scope of the present utility model.
Claims (7)
1. A protoplast culture assistance device, comprising:
the culture tank comprises a culture tank body, wherein a placement assembly is arranged in the culture tank body and comprises an annular mounting frame, and a film is arranged on the inner wall of the annular mounting frame;
the number of the pulling assemblies is two, the two pulling assemblies are symmetrically arranged on the surface of the annular mounting frame, the pulling assemblies comprise pulling rods, one ends of the pulling rods are connected with the mounting frame, and one ends of the mounting frame are connected with the annular mounting frame;
the sealing assemblies are arranged at two ends of the culture tank body respectively.
2. The protoplast culture auxiliary device as claimed in claim 1, wherein a positioning block is connected to the surface of the membrane, a positioning groove adapted to the positioning block is provided on the surface of the annular mounting frame, and a fixing bolt is provided in the positioning block.
3. The protoplast culture auxiliary device as in claim 1, wherein the sealing assembly comprises a sealing cover, a first magnetic ring is connected to one side of the sealing cover, and a second magnetic ring adapted to the first magnetic ring is arranged on the surface of the culture tank body.
4. The protoplast culture auxiliary device as claimed in claim 1, wherein a plurality of air outlet pipes are connected to a surface of the culture tank body.
5. A protoplast culture auxiliary device as claimed in claim 3, wherein a connection tube is connected to one side of the sealing cover.
6. The protoplast culture auxiliary device as claimed in claim 1, wherein a support assembly is provided on a surface of the culture tank body, the support assembly comprises a connection plate, and arc-shaped mounting frames are connected to both sides of the surface of the connection plate.
7. The protoplast culture auxiliary device as claimed in claim 6, wherein the two sides of the bottom of the connecting plate are connected with supporting frames, and the bottoms of the supporting frames are adhered with adhesive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322268032.7U CN220665348U (en) | 2023-08-23 | 2023-08-23 | Protoplast culture auxiliary device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322268032.7U CN220665348U (en) | 2023-08-23 | 2023-08-23 | Protoplast culture auxiliary device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220665348U true CN220665348U (en) | 2024-03-26 |
Family
ID=90326632
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322268032.7U Active CN220665348U (en) | 2023-08-23 | 2023-08-23 | Protoplast culture auxiliary device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220665348U (en) |
-
2023
- 2023-08-23 CN CN202322268032.7U patent/CN220665348U/en active Active
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant |