CN220643095U - Anti-pollution formula culture dish - Google Patents

Anti-pollution formula culture dish Download PDF

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Publication number
CN220643095U
CN220643095U CN202321910249.7U CN202321910249U CN220643095U CN 220643095 U CN220643095 U CN 220643095U CN 202321910249 U CN202321910249 U CN 202321910249U CN 220643095 U CN220643095 U CN 220643095U
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China
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fixedly connected
cover body
pipe
dish
liquid adding
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CN202321910249.7U
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Chinese (zh)
Inventor
包雅丽
孙湛
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Xinjiang Medical University
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Xinjiang Medical University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model belongs to the technical field of cell culture devices, and particularly relates to an anti-pollution culture dish which comprises a dish body and a cover body, wherein the cover body is arranged at the upper end of the dish body, an external thread ring is fixedly connected inside the bottom end of the cover body, a liquid adding pipe is fixedly connected to one side of the upper end of the cover body, a sealing valve mouth I is fixedly connected to the upper end inside the liquid adding pipe, and a sealing valve mouth II is fixedly connected to the middle position inside the liquid adding pipe; the culture dish is characterized in that a branch pipe is fixedly connected to the upper end of the cover body, a spring is movably connected to the inside of the branch pipe, the bottom end of the spring is fixedly connected with the cover body, the upper end of the spring is fixedly connected with a sliding block which is movably connected with the branch pipe, a sliding rod is arranged at the upper end of the sliding block and is movably connected with the branch pipe.

Description

Anti-pollution formula culture dish
Technical Field
The utility model belongs to the technical field of cell culture devices, and particularly relates to an anti-pollution culture dish.
Background
The culture dish is a laboratory vessel for culturing microorganisms or cells, can conveniently carry out the evolution of lecture organisms and the like, for example, the myocardial cells at present, and has very important significance in the research of heart diseases and the treatment of nerve tissues.
When carrying out cell culture, need carry out the interpolation or the multiple addition of culture solution, at present when adding the culture solution, need to open the culture dish lid half or open completely, then add the culture solution into this culture dish through the pipettor, and open the in-process of adding the culture solution at the culture dish, external pollutant gets into in the culture dish easily, causes the influence to the culture of cell, also can cause the influence to experimental accuracy afterwards.
For this purpose, we have an anti-contamination type dish to which the culture solution can be added without opening the dish, to solve the above-mentioned problems.
Disclosure of Invention
In order to solve the problems in the prior art, the utility model provides an anti-pollution culture dish, when a culture solution is added, a dish body and a cover body are not required to be opened, so that external pollutants can be prevented from entering the culture dish to cause internal pollution to a large extent, the culture dish is convenient for culturing and using cells, and the use is better.
In order to achieve the above purpose, the present utility model provides the following technical solutions: an anti-pollution culture dish comprises a dish body and a cover body, wherein the cover body is arranged at the upper end of the dish body, an external thread ring is fixedly connected inside the bottom end of the cover body, a liquid adding pipe is fixedly connected to one side of the upper end of the cover body, a sealing valve mouth I is fixedly connected to the upper end of the inside of the liquid adding pipe, and a sealing valve mouth II is fixedly connected to the middle position of the inside of the liquid adding pipe;
the upper end fixedly connected with branch pipe of lid, the inside swing joint of branch pipe has the spring, the bottom and the lid fixed connection of spring, the upper end fixedly connected with slider and branch pipe swing joint of spring, the upper end of slider be provided with the slide bar just slide bar and branch pipe swing joint, fixed mounting has the pivot between slide bar and the slider, the upper end one side fixedly connected with connecting rod of slide bar, one side fixedly connected with T shape sealing plug of connecting rod, just T shape sealing plug is connected with the upper end block of liquid feeding pipe.
As an optimal technical scheme of the anti-pollution culture dish, the periphery of the outer part of the bottom end of the dish body is fixedly connected with a seat ring.
As an optimal technical scheme of the anti-pollution culture dish, the periphery of the upper end of the dish body is fixedly connected with an inner sealing gasket.
As an optimal technical scheme of the anti-pollution culture dish, the periphery of the outer wall of the bottom end of the dish body is fixedly connected with the first outer sealing ring, and the periphery of the outer wall of the bottom end of the cover body is fixedly connected with the second outer sealing ring.
As an optimal technical scheme of the anti-pollution culture dish, the T-shaped sealing plug is of a convex structure, an inner sealing sleeve is fixedly connected to the periphery of the outer part of the bottom end of the T-shaped sealing plug, and the inner sealing sleeve is extruded and attached to the periphery of the inner wall of the liquid adding pipe.
As an optimal technical scheme of the anti-pollution culture dish, the periphery of the upper end of the liquid adding pipe is fixedly connected with the outer sealing gasket, and the periphery of the bottom end of the T-shaped sealing plug is extruded and attached with the outer sealing gasket.
Compared with the prior art, the utility model has the beneficial effects that:
1. when the culture solution is added into the dish body, the cover body is not required to be opened, so that a large amount of external pollutants can be prevented from entering the dish body, the liquid adding pipe on the cover body is only required to be inserted into the suction nozzle of the liquid adding device, the first sealing valve mouth and the second sealing valve mouth in the liquid adding pipe can be opened by the suction nozzle of the liquid adding device and tightly wrap the periphery of the outer wall of the suction nozzle, the external pollutants can be prevented from entering the dish body through the liquid adding pipe in the process of adding the culture solution, then a proper amount of culture solution is injected into the dish body through the liquid adding device, the suction nozzle of the liquid adding device is pulled out, and the first sealing valve mouth and the second sealing valve mouth in the liquid adding pipe can be reset and closed by themselves according to the deformation performance of the suction nozzle of the liquid adding pipe, so that the internal pipeline of the liquid adding pipe is prevented from entering the external impurities, the operation is simple, and the use is convenient.
2. In the use process, the upper pipe orifice of the liquid adding pipe after liquid adding can be covered by the T-shaped sealing plug, so that the liquid adding pipe can be completely sealed, the sealing performance is better, and the T-shaped sealing plug is convenient to pull through the matched arrangement of the branch pipe, the spring, the sliding block, the rotating shaft, the sliding rod and the connecting rod, so that the liquid adding pipe can be conveniently opened and closed.
Drawings
The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the embodiments of the utility model, serve to explain the utility model. In the drawings:
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a schematic view of the bottom structure of FIG. 1;
FIG. 3 is a schematic view of the open state structure of FIG. 1;
FIG. 4 is a schematic cross-sectional view of FIG. 3;
FIG. 5 is a schematic cross-sectional view of the cover of FIG. 4;
FIG. 6 is a schematic view showing the arrangement state of the first sealing valve nozzle and the second sealing valve nozzle in the liquid filling pipe.
In the figure: 1. a dish body; 2. a seat ring; 3. an inner sealing gasket; 4. an outer seal collar I; 5. a cover body; 6. an external threaded ring; 7. an outer sealing ferrule II; 8. a liquid adding tube; 9. sealing the valve mouth I; 10. sealing a valve mouth II; 11. an outer sealing gasket; 12. a branch pipe; 13. a spring; 14. a slide block; 15. a rotating shaft; 16. a slide bar; 17. a connecting rod; 18. a T-shaped sealing plug; 19. an inner sealing sleeve.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Examples
Referring to fig. 1-6, the present utility model provides the following technical solutions: the utility model provides an anti-pollution formula culture dish, including the disk body 1 and lid 5, the lid 5 sets up the upper end at the disk body 1, the inside fixedly connected with external screw thread ring 6 of bottom of lid 5, the upper end one side fixedly connected with liquid feeding pipe 8 of lid 5, the inside upper end fixedly connected with sealing valve mouth one 9 of liquid feeding pipe 8, the inside intermediate position fixedly connected with sealing valve mouth two 10 of liquid feeding pipe 8, wherein, disk body 1, lid 5, external screw thread ring 6 and liquid feeding pipe 8 are transparent plastic material, sealing valve mouth one 9 and sealing valve mouth two 10 are transparent silica gel material, so that the observation of cell culture state in this culture dish, external screw thread ring 6 is used for lid 5 to be connected with the rotation of disk body 1, and offer on the inner wall of disk body 1 with external screw thread assorted internal screw thread on the external wall of external screw thread ring 6, its screw thread structure is not specifically shown in the figure, liquid feeding pipe 8 communicates with each other with lid 5, so that the culture solution passes through lid 5 by liquid feeding pipe 8 and gets into in the body 1, sealing valve mouth one 9 and sealing valve mouth two 10 are by the transparent silica gel pad of sector.
In this embodiment, when the culture solution is added into the dish body 1, the cover body 5 is not required to be opened, so that a large amount of external pollutants can be prevented from entering the dish body 1, the liquid adding pipe 8 on the cover body 5 is only required to be inserted into the liquid adding pipe nozzle, the sealing valve nozzle I9 and the sealing valve nozzle II 10 in the liquid adding pipe 8 can be opened by the liquid adding pipe nozzle and tightly wrap the periphery of the outer wall of the nozzle, the external pollutants can be prevented from entering the dish body 1 through the liquid adding pipe 8 in the process of adding the culture solution, then a proper amount of culture solution is injected into the dish body 1 through the liquid adding pipe, and after the liquid adding pipe nozzle is pulled out, the sealing valve nozzle I9 and the sealing valve nozzle II 10 in the liquid adding pipe 8 can be reset and closed by themselves according to the deformation performance of the liquid adding pipe 8, so that the external impurities can be prevented from entering the pipeline inside the liquid adding pipe 8, and the culture solution is easy to operate and convenient to use.
Further, referring to fig. 1, 2, 3, 4, 5 and 6, the upper end of the cover 5 is fixedly connected with a branch pipe 12, the inside of the branch pipe 12 is movably connected with a spring 13, the bottom end of the spring 13 is fixedly connected with the cover 5, the upper end of the spring 13 is fixedly connected with a slide block 14, the slide block 14 is movably connected with the branch pipe 12, the upper end of the slide block 14 is provided with a slide bar 16, the slide bar 16 is movably connected with the branch pipe 12, a rotating shaft 15 is fixedly arranged between the slide bar 16 and the slide block 14, one side of the upper end of the slide bar 16 is fixedly connected with a connecting rod 17, one side of the connecting rod 17 is fixedly connected with a T-shaped sealing plug 18, the T-shaped sealing plug 18 is in clamping connection with the upper end of the liquid adding pipe 8, wherein the branch pipe 12, the slide block 14, the slide bar 16, the connecting rod 17 and the T-shaped sealing plug 18 are all made of transparent plastic materials, so that the observation of the cell culture state in the culture dish is convenient, when in use, the T-shaped sealing plug 18 is pulled out of the liquid adding pipe 8 through the connecting rod 17, the sliding rod 16, the rotating shaft 15, the sliding block 14 and the spring 13, then the T-shaped sealing plug 18, the connecting rod 17 and the sliding rod 16 can be rotated to one side and loosened through the rotating shaft 15 between the sliding rod 16 and the sliding block 14, the pipe orifice at the upper end of the sealing valve mouth 9 is exposed, after the culture dish is used, the T-shaped sealing plug 18 is lifted and rotated in the same way, the T-shaped sealing plug 18 is positioned above the liquid adding pipe 8 and then loosened, and under the elastic restoring force of the spring 13, the T-shaped sealing plug 18 can be clamped in the upper end of the liquid adding pipe 8 by itself, so that the pipe orifice at the upper end of the liquid adding pipe 8 is blocked, and external foreign matters can be prevented from entering.
In this embodiment, in the use process of the utility model, the upper end pipe orifice of the liquid adding pipe 8 after liquid adding can be further covered by the T-shaped sealing plug 18, so that the liquid adding pipe 8 can be completely sealed, the tightness is better, and the T-shaped sealing plug 18 is convenient to pull through the cooperation of the branch pipe 12, the spring 13, the sliding block 14, the rotating shaft 15, the sliding rod 16 and the connecting rod 17, so that the liquid adding pipe 8 can be conveniently opened and closed.
Further, referring to fig. 1, 2, 3, 4, 5 and 6, a seat ring 2 is fixedly connected around the bottom end of the dish body 1; the periphery of the upper end of the dish body 1 is fixedly connected with an inner sealing gasket 3, the periphery of the outer wall of the bottom end of the dish body 1 is fixedly connected with an outer sealing ferrule I4, and the periphery of the outer wall of the bottom end of the cover body 5 is fixedly connected with an outer sealing ferrule II 7; the T-shaped sealing plug 18 is of a convex structure, an inner sealing sleeve 19 is fixedly connected to the periphery of the outer part of the bottom end of the T-shaped sealing plug 18, and the inner sealing sleeve 19 is extruded and attached to the periphery of the inner wall of the liquid adding tube 8; the upper end of liquid feeding pipe 8 fixedly connected with external seal packing ring 11 all around, and T shape sealing plug 18's bottom all around with external seal packing ring 11 extrusion laminating, wherein, seat circle 2, interior seal packing ring 3, external seal lasso one 4, external seal lasso two 7, interior seal cover 19 and external seal packing ring 11 are transparent silica gel material, so that the observation of cell culture state in this culture dish, when using, through the setting of dish body 1 bottom seat circle 2, can carry out flexible protection all around to dish body 1's bottom, avoid colliding with and cause the damage between this culture dish bottom and the operation panel mesa, when using, through the bottom of lid 5 all around with the internal seal packing ring 3 extrusion laminating all around of dish body 1 upper end all around, can realize lid 5 and dish body 1 and the preliminary seal of junction, and through the external seal lasso two 7 all around outside the lid 5 and the external seal 4 extrusion laminating all around of dish body 1, can realize lid 5 and dish body 1 and the further seal after the connection of rotating, with reach inside and outside dual seal's effect, the leakproofness is better, when using, through T shape sealing plug 18 outer seal ring 8 and seal 8 all around with the sealing plug 8 of liquid feeding pipe 8, seal 8 all around and seal pipe 8 are laminated completely with the extrusion sealing plug 8, seal pipe 8 is realized all around in the end of sealing plug 8 and sealing pipe 8 is laminated.
The utility model has the following application flow and working principle: the screwing connection of the cover body 5 and the dish body 1 can be completed through the external thread ring 6 at the bottom end of the cover body 5, meanwhile, the preliminary sealing at the screwing connection position of the cover body 5 and the dish body 1 can be realized through the extrusion lamination of the periphery of the bottom end of the cover body 5 and the internal sealing gasket 3 at the periphery of the upper end of the dish body 1, and the further sealing after the screwing connection of the cover body 5 and the dish body 1 can be realized through the extrusion lamination of the external sealing ferrule II 7 at the periphery of the outer part of the cover body 5 and the external sealing ferrule I4 at the periphery of the outer part of the dish body 1, so that the effect of internal and external double sealing is achieved, and the sealing performance is better;
then lifting the T-shaped sealing plug 18, the sliding rod 16, the rotating shaft 15, the sliding block 14 and the spring 13 through the connecting rod 17, pulling the T-shaped sealing plug 18 out of the liquid adding pipe 8, and then rotating the T-shaped sealing plug 18, the connecting rod 17 and the sliding rod 16 to one side and loosening through the rotating shaft 15 between the sliding rod 16 and the sliding block 14, so that a pipe orifice at the upper end of the sealing valve mouth I9 is exposed;
then the suction nozzle of the liquid-transfering device which is used for sucking the culture solution is inserted into the liquid-transfering tube 8, the first sealing valve nozzle 9 and the second sealing valve nozzle 10 in the liquid-transfering tube 8 are propped up by the suction nozzle of the liquid-transfering device, and the periphery of the outer wall of the suction nozzle is tightly wrapped, so that external pollutants can be prevented from entering the dish body 1 from the liquid-transfering tube 8 in the process of adding the culture solution, then a proper amount of culture solution is injected into the dish body 1 from the liquid-transfering device, after the suction nozzle of the liquid-transfering device is pulled out, the first sealing valve nozzle 9 and the second sealing valve nozzle 10 in the liquid-transfering tube 8 can be automatically reset and closed according to the deformation property of the suction nozzle, so that the inner pipeline of the liquid-transfering tube 8 is blocked, and external foreign matters are prevented from entering;
finally, the T-shaped sealing plug 18 is lifted up and rotated in the same way, the T-shaped sealing plug 18 is positioned above the liquid adding pipe 8 and then loosened, under the elastic restoring force of the spring 13, the T-shaped sealing plug 18 can be clamped inside the upper end of the liquid adding pipe 8 to finish the plugging of the pipe orifice at the upper end of the liquid adding pipe 8, the inner sealing sleeve 19 outside the T-shaped sealing plug 18 is extruded and attached to the inner wall of the upper end of the liquid adding pipe 8, the periphery of the bottom end of the T-shaped sealing plug 18 is extruded and attached to the outer sealing gasket 11 at the upper end of the liquid adding pipe 8, the sealing effect after the T-shaped sealing plug 18 is clamped and connected with the liquid adding pipe 8 is improved, so that the complete sealing of the liquid adding pipe 8 can be realized, external foreign matters can be avoided entering the liquid adding culture dish.
In this embodiment, the details not described in detail are in the category of the prior art and the common general knowledge.
Finally, it should be noted that: the above is only a preferred embodiment of the present utility model, and the present utility model is not limited thereto, but it is to be understood that the present utility model is described in detail with reference to the foregoing embodiments, and modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims (6)

1. An anti-pollution culture dish, characterized in that: the novel water-filling bowl comprises a bowl body (1) and a cover body (5), wherein the cover body (5) is arranged at the upper end of the bowl body (1), an external thread ring (6) is fixedly connected inside the bottom end of the cover body (5), a liquid filling pipe (8) is fixedly connected to one side of the upper end of the cover body (5), a sealing valve mouth I (9) is fixedly connected to the upper end of the liquid filling pipe (8), and a sealing valve mouth II (10) is fixedly connected to the middle position inside the liquid filling pipe (8);
the upper end of the cover body (5) is fixedly connected with a branch pipe (12), the inside of the branch pipe (12) is movably connected with a spring (13), the bottom end of the spring (13) is fixedly connected with the cover body (5), the upper end of the spring (13) is fixedly connected with a sliding block (14) and the sliding block (14) is movably connected with the branch pipe (12), the upper end of the sliding block (14) is provided with a sliding rod (16) and the sliding rod (16) is movably connected with the branch pipe (12), a rotating shaft (15) is fixedly arranged between the sliding rod (16) and the sliding block (14), a connecting rod (17) is fixedly connected to one side of the upper end of the sliding rod (16), a T-shaped sealing plug (18) is fixedly connected to one side of the connecting rod (17), and the T-shaped sealing plug (18) is connected with the upper end of the liquid adding pipe (8) in a clamping mode.
2. The anti-contamination culture dish as claimed in claim 1, wherein: the outer periphery of the bottom end of the dish body (1) is fixedly connected with a seat ring (2).
3. An anti-contamination culture dish according to claim 2, wherein: an inner sealing gasket (3) is fixedly connected to the periphery of the upper end of the dish body (1).
4. The anti-contamination culture dish as claimed in claim 1, wherein: the outer wall of the bottom end of the dish body (1) is fixedly connected with an outer sealing ring I (4), and the outer wall of the bottom end of the cover body (5) is fixedly connected with an outer sealing ring II (7).
5. The anti-contamination culture dish as claimed in claim 1, wherein: the T-shaped sealing plug (18) is of a convex structure, an inner sealing sleeve (19) is fixedly connected to the periphery of the outer portion of the bottom end of the T-shaped sealing plug (18), and the inner sealing sleeve (19) is extruded and attached to the periphery of the inner wall of the liquid adding tube (8).
6. The anti-contamination culture dish according to claim 5, wherein: the upper end of the liquid adding pipe (8) is fixedly connected with an outer sealing gasket (11) around, and the periphery of the bottom end of the T-shaped sealing plug (18) is extruded and attached to the outer sealing gasket (11).
CN202321910249.7U 2023-07-20 2023-07-20 Anti-pollution formula culture dish Active CN220643095U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202321910249.7U CN220643095U (en) 2023-07-20 2023-07-20 Anti-pollution formula culture dish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202321910249.7U CN220643095U (en) 2023-07-20 2023-07-20 Anti-pollution formula culture dish

Publications (1)

Publication Number Publication Date
CN220643095U true CN220643095U (en) 2024-03-22

Family

ID=90287436

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202321910249.7U Active CN220643095U (en) 2023-07-20 2023-07-20 Anti-pollution formula culture dish

Country Status (1)

Country Link
CN (1) CN220643095U (en)

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